Restriction map for
pFN21AE5757
(Linear) (Six-base) MAPSORT of: KIEE5757.seq Check: 650 from: 1 to: 2277
KIEE5757.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1962 2277
Size: 1962 315
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1261 1435 1729 1982 2277
Size: 1261 174 294 253 295
Fragments arranged by size:
1261 295 294 253 174
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1117 1670 2277
Size: 1117 553 607
Fragments arranged by size:
1117 607 553
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 52 84 2277
Size: 52 32 2193
Fragments arranged by size:
2193 52 32
AlwNI CAG_nnn'CTG
Cuts at: 0 553 829 1466 1617 1843 2277
Size: 553 276 637 151 226 434
Fragments arranged by size:
637 553 434 276 226 151
ApaI G_GGCC'C
Cuts at: 0 183 2277
Size: 183 2094
ApoI r'AATT_y
Cuts at: 0 797 2277
Size: 797 1480
AseI AT'TA_AT
Cuts at: 0 346 2277
Size: 346 1931
AvaI C'yCGr_G
Cuts at: 0 1328 2277
Size: 1328 949
AvrII C'CTAG_G
Cuts at: 0 436 2277
Size: 436 1841
BamHI G'GATC_C
Cuts at: 0 1772 2277
Size: 1772 505
BanI G'GyrC_C
Cuts at: 0 1306 1899 2277
Size: 1306 593 378
Fragments arranged by size:
1306 593 378
BanII G_rGCy'C
Cuts at: 0 183 1930 2277
Size: 183 1747 347
Fragments arranged by size:
1747 347 183
BbsI GAAGACnn'nnnn_
Cuts at: 0 397 2277
Size: 397 1880
BclI T'GATC_A
Cuts at: 0 843 2277
Size: 843 1434
BglI GCCn_nnn'nGGC
Cuts at: 0 325 2277
Size: 325 1952
Bme1580I G_kGCm'C
Cuts at: 0 183 292 1904 2277
Size: 183 109 1612 373
Fragments arranged by size:
1612 373 183 109
BmrI ACTGGGnnnn_n'
Cuts at: 0 1137 2277
Size: 1137 1140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1175 2277
Size: 79 1096 1102
Fragments arranged by size:
1102 1096 79
Bpu10I CC'TnA_GC
Cuts at: 0 361 2162 2277
Size: 361 1801 115
Fragments arranged by size:
1801 361 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1139 1219 1414 2277
Size: 169 970 80 195 863
Fragments arranged by size:
970 863 195 169 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 813 1571 1707 2277
Size: 813 758 136 570
Fragments arranged by size:
813 758 570 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 52 82 2277
Size: 52 30 2195
Fragments arranged by size:
2195 52 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 868 943 2271 2277
Size: 868 75 1328 6
Fragments arranged by size:
1328 868 75 6
BseYI C'CCAG_C
Cuts at: 0 1348 2277
Size: 1348 929
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1456 2277
Size: 1456 821
BsiHKAI G_wGCw'C
Cuts at: 0 1649 2244 2277
Size: 1649 595 33
Fragments arranged by size:
1649 595 33
BsmI GAATG_Cn'
Cuts at: 0 1119 2277
Size: 1119 1158
Bsp1286I G_dGCh'C
Cuts at: 0 183 292 1649 1904 1930 2244 2277
Size: 183 109 1357 255 26 314 33
Fragments arranged by size:
1357 314 255 183 109 33 26
BspHI T'CATG_A
Cuts at: 0 1148 2277
Size: 1148 1129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 490 1962 2133 2277
Size: 490 1472 171 144
Fragments arranged by size:
1472 490 171 144
BsrFI r'CCGG_y
Cuts at: 0 4 84 1226 2277
Size: 4 80 1142 1051
Fragments arranged by size:
1142 1051 80 4
BssSI C'ACGA_G
Cuts at: 0 1648 2277
Size: 1648 629
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2248 2277
Size: 2248 29
BstEII G'GTnAC_C
Cuts at: 0 780 1251 2277
Size: 780 471 1026
Fragments arranged by size:
1026 780 471
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1072 2277
Size: 1072 1205
BstYI r'GATC_y
Cuts at: 0 1772 1798 2277
Size: 1772 26 479
Fragments arranged by size:
1772 479 26
BtgI C'CryG_G
Cuts at: 0 566 2277
Size: 566 1711
BtsI GCAGTG_nn'
Cuts at: 0 54 100 846 2277
Size: 54 46 746 1431
Fragments arranged by size:
1431 746 54 46
EaeI y'GGCC_r
Cuts at: 0 2 1224 1388 2277
Size: 2 1222 164 889
Fragments arranged by size:
1222 889 164 2
EarI CTCTTCn'nnn_
Cuts at: 0 668 680 749 755 799 964 1705 1909
Size: 668 12 69 6 44 165 741 204
Cuts at: 1909 1990 2277
Size: 81 287
Fragments arranged by size:
741 668 287 204 165 81 69 44
12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1660 2277
Size: 1660 617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1175 1261 1435 1729 1982 2277
Size: 79 1096 86 174 294 253 295
Fragments arranged by size:
1096 295 294 253 174 86 79
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2234 2277
Size: 2234 43
EcoO109I rG'GnC_Cy
Cuts at: 0 179 180 2007 2032 2227 2277
Size: 179 1 1827 25 195 50
Fragments arranged by size:
1827 195 179 50 25 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 114 1066 1260 2277
Size: 114 952 194 1017
Fragments arranged by size:
1017 952 194 114
FseI GG_CCGG'CC
Cuts at: 0 8 2277
Size: 8 2269
FspI TGC'GCA
Cuts at: 0 1552 2277
Size: 1552 725
FspAI rTGC'GCAy
Cuts at: 0 1552 2277
Size: 1552 725
HaeII r_GCGC'y
Cuts at: 0 2017 2277
Size: 2017 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 2131 2163 2277
Size: 580 32 1519 32 114
Fragments arranged by size:
1519 580 114 32 32
HincII GTy'rAC
Cuts at: 0 1242 2277
Size: 1242 1035
HindIII A'AGCT_T
Cuts at: 0 1273 2277
Size: 1273 1004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1089 2277
Size: 1089 1188
MscI TGG'CCA
Cuts at: 0 1390 2277
Size: 1390 887
MslI CAynn'nnrTG
Cuts at: 0 523 1652 2277
Size: 523 1129 625
Fragments arranged by size:
1129 625 523
MspA1I CmG'CkG
Cuts at: 0 57 2196 2277
Size: 57 2139 81
Fragments arranged by size:
2139 81 57
NaeI GCC'GGC
Cuts at: 0 6 1228 2277
Size: 6 1222 1049
Fragments arranged by size:
1222 1049 6
NgoMIV G'CCGG_C
Cuts at: 0 4 1226 2277
Size: 4 1222 1051
Fragments arranged by size:
1222 1051 4
NspI r_CATG'y
Cuts at: 0 131 1657 2277
Size: 131 1526 620
Fragments arranged by size:
1526 620 131
PasI CC'CwG_GG
Cuts at: 0 1617 2277
Size: 1617 660
PfoI T'CCnGG_A
Cuts at: 0 419 2277
Size: 419 1858
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 2277
Size: 52 32 2193
Fragments arranged by size:
2193 52 32
PpuMI rG'GwC_Cy
Cuts at: 0 2007 2227 2277
Size: 2007 220 50
Fragments arranged by size:
2007 220 50
PspOMI G'GGCC_C
Cuts at: 0 179 2277
Size: 179 2098
PstI C_TGCA'G
Cuts at: 0 1365 2277
Size: 1365 912
PvuII CAG'CTG
Cuts at: 0 57 2196 2277
Size: 57 2139 81
Fragments arranged by size:
2139 81 57
SfcI C'TryA_G
Cuts at: 0 608 1361 1490 2277
Size: 608 753 129 787
Fragments arranged by size:
787 753 608 129
SmlI C'TyrA_G
Cuts at: 0 184 1154 1234 1429 2277
Size: 184 970 80 195 848
Fragments arranged by size:
970 848 195 184 80
SpeI A'CTAG_T
Cuts at: 0 1531 2277
Size: 1531 746
SphI G_CATG'C
Cuts at: 0 1657 2277
Size: 1657 620
StyI C'CwwG_G
Cuts at: 0 436 1955 2277
Size: 436 1519 322
Fragments arranged by size:
1519 436 322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1962 2277
Size: 1962 315
XmnI GAAnn'nnTTC
Cuts at: 0 170 2277
Size: 170 2107
Enzymes that do cut:
AarI AcuI AlfI AloI AlwNI ApaI ApoI AseI
AvaI AvrII BamHI BanI BanII BbsI BclI BglI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI
BssSI BstAPI BstEII BstXI BstYI BtgI BtsI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII MmeI MscI MslI
MspA1I NaeI NgoMIV NspI PasI PfoI PpiI PpuMI
PspOMI PstI PvuII SfcI SmlI SpeI SphI StyI
XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaLI AscI AsiSI BaeI BbeI BbvCI
BcgI BciVI BfrBI BglII BlpI BmgBI BmtI BplI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI
BsrBI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI
EcoRV HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI ZraI