Restriction map for
pFN21AE5754
(Linear) (Six-base) MAPSORT of: KIEE5754.seq Check: 2846 from: 1 to: 2208
KIEE5754.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 2051 2208
Size: 877 1174 157
Fragments arranged by size:
1174 877 157
AflII C'TTAA_G
Cuts at: 0 432 2208
Size: 432 1776
AflIII A'CryG_T
Cuts at: 0 363 2208
Size: 363 1845
AhdI GACnn_n'nnGTC
Cuts at: 0 1837 2208
Size: 1837 371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 780 2208
Size: 780 1428
ApoI r'AATT_y
Cuts at: 0 284 469 475 623 882 966 1015 1046
Size: 284 185 6 148 259 84 49 31
Cuts at: 1046 1092 1222 1496 2181 2208
Size: 46 130 274 685 27
Fragments arranged by size:
685 284 274 259 185 148 130 84
49 46 31 27 6
AseI AT'TA_AT
Cuts at: 0 121 2208
Size: 121 2087
AvaI C'yCGr_G
Cuts at: 0 902 1545 2208
Size: 902 643 663
Fragments arranged by size:
902 663 643
BamHI G'GATC_C
Cuts at: 0 298 897 2208
Size: 298 599 1311
Fragments arranged by size:
1311 599 298
BciVI GTATCCnnnnn_n'
Cuts at: 0 785 2208
Size: 785 1423
BclI T'GATC_A
Cuts at: 0 99 2208
Size: 99 2109
BglII A'GATC_T
Cuts at: 0 373 543 2208
Size: 373 170 1665
Fragments arranged by size:
1665 373 170
BlpI GC'TnA_GC
Cuts at: 0 1688 2208
Size: 1688 520
BmgBI CAC'GTC
Cuts at: 0 697 2208
Size: 697 1511
BmrI ACTGGGnnnn_n'
Cuts at: 0 1245 2114 2208
Size: 1245 869 94
Fragments arranged by size:
1245 869 94
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1584 1619 2132 2208
Size: 1584 35 513 76
Fragments arranged by size:
1584 513 76 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1102 2208
Size: 1102 1106
BsaAI yAC'GTr
Cuts at: 0 974 1296 2208
Size: 974 322 912
Fragments arranged by size:
974 912 322
BsaBI GATnn'nnATC
Cuts at: 0 923 1535 2208
Size: 923 612 673
Fragments arranged by size:
923 673 612
BsaHI Gr'CG_yC
Cuts at: 0 128 2208
Size: 128 2080
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2081 2208
Size: 2081 127
BsmBI CGTCTCn'nnnn_
Cuts at: 0 545 2208
Size: 545 1663
BsrDI GCAATG_nn'
Cuts at: 0 752 2208
Size: 752 1456
BsrGI T'GTAC_A
Cuts at: 0 1397 2208
Size: 1397 811
BstAPI GCAn_nnn'nTGC
Cuts at: 0 636 2208
Size: 636 1572
BstYI r'GATC_y
Cuts at: 0 298 373 543 897 1933 2208
Size: 298 75 170 354 1036 275
Fragments arranged by size:
1036 354 298 275 170 75
BtsI GCAGTG_nn'
Cuts at: 0 2024 2208
Size: 2024 184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 347 382 2208
Size: 347 35 1826
Fragments arranged by size:
1826 347 35
DraI TTT'AAA
Cuts at: 0 1020 2208
Size: 1020 1188
DraIII CAC_nnn'GTG
Cuts at: 0 349 1723 1808 2208
Size: 349 1374 85 400
Fragments arranged by size:
1374 400 349 85
EaeI y'GGCC_r
Cuts at: 0 838 2208
Size: 838 1370
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1565 2208
Size: 1565 643
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 1584 1619 2051 2132 2208
Size: 877 707 35 432 81 76
Fragments arranged by size:
877 707 432 81 76 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1661 2208
Size: 1661 547
EcoO109I rG'GnC_Cy
Cuts at: 0 2016 2208
Size: 2016 192
EcoRI G'AATT_C
Cuts at: 0 966 2208
Size: 966 1242
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1363 1395 1823 1855 2128 2160 2187 2208
Size: 1363 32 428 32 273 32 27 21
Fragments arranged by size:
1363 428 273 32 32 32 27 21
HincII GTy'rAC
Cuts at: 0 43 1353 1773 2208
Size: 43 1310 420 435
Fragments arranged by size:
1310 435 420 43
MfeI C'AATT_G
Cuts at: 0 769 2208
Size: 769 1439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 1149 1182 2110 2208
Size: 734 415 33 928 98
Fragments arranged by size:
928 734 415 98 33
MscI TGG'CCA
Cuts at: 0 840 2208
Size: 840 1368
MslI CAynn'nnrTG
Cuts at: 0 416 1753 1892 2208
Size: 416 1337 139 316
Fragments arranged by size:
1337 416 316 139
NdeI CA'TA_TG
Cuts at: 0 1368 2208
Size: 1368 840
NspI r_CATG'y
Cuts at: 0 367 717 2208
Size: 367 350 1491
Fragments arranged by size:
1491 367 350
PasI CC'CwG_GG
Cuts at: 0 1261 1875 2208
Size: 1261 614 333
Fragments arranged by size:
1261 614 333
PciI A'CATG_T
Cuts at: 0 363 2208
Size: 363 1845
PflMI CCAn_nnn'nTGG
Cuts at: 0 180 1759 2208
Size: 180 1579 449
Fragments arranged by size:
1579 449 180
PpuMI rG'GwC_Cy
Cuts at: 0 2016 2208
Size: 2016 192
PsiI TTA'TAA
Cuts at: 0 466 2208
Size: 466 1742
PstI C_TGCA'G
Cuts at: 0 1494 2208
Size: 1494 714
SfcI C'TryA_G
Cuts at: 0 303 440 1490 1661 1778 2208
Size: 303 137 1050 171 117 430
Fragments arranged by size:
1050 430 303 171 137 117
SmlI C'TyrA_G
Cuts at: 0 432 1081 2208
Size: 432 649 1127
Fragments arranged by size:
1127 649 432
SnaBI TAC'GTA
Cuts at: 0 974 1296 2208
Size: 974 322 912
Fragments arranged by size:
974 912 322
StyI C'CwwG_G
Cuts at: 0 726 1311 1737 2208
Size: 726 585 426 471
Fragments arranged by size:
726 585 471 426
TatI w'GTAC_w
Cuts at: 0 315 1194 1397 2208
Size: 315 879 203 811
Fragments arranged by size:
879 811 315 203
XbaI T'CTAG_A
Cuts at: 0 1158 2208
Size: 1158 1050
XmnI GAAnn'nnTTC
Cuts at: 0 1049 1499 2208
Size: 1049 450 709
Fragments arranged by size:
1049 709 450
Enzymes that do cut:
AcuI AflII AflIII AhdI AlfI ApoI AseI AvaI
BamHI BciVI BclI BglII BlpI BmgBI BmrI BpmI
BpuEI BsaAI BsaBI BsaHI BseRI BsmBI BsrDI BsrGI
BstAPI BstYI BtsI CspCI DraI DraIII EaeI EciI
Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII MfeI MmeI
MscI MslI NdeI NspI PasI PciI PflMI PpuMI
PsiI PstI SfcI SmlI SnaBI StyI TatI XbaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AleI
AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI
BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglI
Bme1580I BmtI BplI Bpu10I BsaI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI DrdI EagI EarI
EcoICRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MluI MspA1I NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XcmI
XhoI XmaI ZraI