Restriction map for
pFN21AE5750
(Linear) (Six-base) MAPSORT of: KIEE5750.seq Check: 648 from: 1 to: 2076
KIEE5750.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 403 2076
Size: 403 1673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 1063 1573 2076
Size: 236 827 510 503
Fragments arranged by size:
827 510 503 236
AfeI AGC'GCT
Cuts at: 0 1058 1594 2076
Size: 1058 536 482
Fragments arranged by size:
1058 536 482
AlwNI CAG_nnn'CTG
Cuts at: 0 1562 1951 2076
Size: 1562 389 125
Fragments arranged by size:
1562 389 125
ApaI G_GGCC'C
Cuts at: 0 1005 1942 2076
Size: 1005 937 134
Fragments arranged by size:
1005 937 134
AvaI C'yCGr_G
Cuts at: 0 23 464 996 1252 2076
Size: 23 441 532 256 824
Fragments arranged by size:
824 532 441 256 23
BamHI G'GATC_C
Cuts at: 0 546 2076
Size: 546 1530
BanI G'GyrC_C
Cuts at: 0 79 677 882 1108 1410 1522 2076
Size: 79 598 205 226 302 112 554
Fragments arranged by size:
598 554 302 226 205 112 79
BanII G_rGCy'C
Cuts at: 0 24 230 472 1005 1668 1830 1942 1994
Size: 24 206 242 533 663 162 112 52
Cuts at: 1994 2076
Size: 82
Fragments arranged by size:
663 533 242 206 162 112 82 52
24
BbeI G_GCGC'C
Cuts at: 0 83 2076
Size: 83 1993
BbvCI CC'TCA_GC
Cuts at: 0 424 1762 2076
Size: 424 1338 314
Fragments arranged by size:
1338 424 314
BciVI GTATCCnnnnn_n'
Cuts at: 0 265 2076
Size: 265 1811
BclI T'GATC_A
Cuts at: 0 416 2076
Size: 416 1660
BglI GCCn_nnn'nGGC
Cuts at: 0 211 1798 2076
Size: 211 1587 278
Fragments arranged by size:
1587 278 211
BglII A'GATC_T
Cuts at: 0 494 2076
Size: 494 1582
Bme1580I G_kGCm'C
Cuts at: 0 1005 1111 1485 1942 2076
Size: 1005 106 374 457 134
Fragments arranged by size:
1005 457 374 134 106
BmrI ACTGGGnnnn_n'
Cuts at: 0 138 999 1472 1521 2076
Size: 138 861 473 49 555
Fragments arranged by size:
861 555 473 138 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 195 2076
Size: 195 1881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 187 229 262 799 1073 1225 1640
Size: 27 160 42 33 537 274 152 415
Cuts at: 1640 2076
Size: 436
Fragments arranged by size:
537 436 415 274 160 152 42 33
27
Bpu10I CC'TnA_GC
Cuts at: 0 424 1762 2076
Size: 424 1338 314
Fragments arranged by size:
1338 424 314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 2076
Size: 256 1820
BsaI GGTCTCn'nnnn_
Cuts at: 0 183 2076
Size: 183 1893
BsaAI yAC'GTr
Cuts at: 0 729 2076
Size: 729 1347
BsaHI Gr'CG_yC
Cuts at: 0 80 2076
Size: 80 1996
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 692 722 1198 1228 2076
Size: 692 30 476 30 848
Fragments arranged by size:
848 692 476 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 940 1104 1750 2076
Size: 184 756 164 646 326
Fragments arranged by size:
756 646 326 184 164
BseYI C'CCAG_C
Cuts at: 0 1181 1245 1387 1484 1661 1847 1993 2076
Size: 1181 64 142 97 177 186 146 83
Fragments arranged by size:
1181 186 177 146 142 97 83 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 1358 1741 1893 2076
Size: 375 983 383 152 183
Fragments arranged by size:
983 383 375 183 152
BsiHKAI G_wGCw'C
Cuts at: 0 24 230 1656 2076
Size: 24 206 1426 420
Fragments arranged by size:
1426 420 206 24
BsmI GAATG_Cn'
Cuts at: 0 438 1578 2076
Size: 438 1140 498
Fragments arranged by size:
1140 498 438
BsmBI CGTCTCn'nnnn_
Cuts at: 0 712 2076
Size: 712 1364
Bsp1286I G_dGCh'C
Cuts at: 0 24 230 472 1005 1111 1485 1656 1668
Size: 24 206 242 533 106 374 171 12
Cuts at: 1668 1830 1942 1994 2076
Size: 162 112 52 82
Fragments arranged by size:
533 374 242 206 171 162 112 106
82 52 24 12
BspHI T'CATG_A
Cuts at: 0 1986 2076
Size: 1986 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 403 1502 2076
Size: 403 1099 574
Fragments arranged by size:
1099 574 403
BsrBI CCG'CTC
Cuts at: 0 795 2076
Size: 795 1281
BsrDI GCAATG_nn'
Cuts at: 0 336 2076
Size: 336 1740
BsrFI r'CCGG_y
Cuts at: 0 174 772 888 1047 1792 2076
Size: 174 598 116 159 745 284
Fragments arranged by size:
745 598 284 174 159 116
BsrGI T'GTAC_A
Cuts at: 0 641 2076
Size: 641 1435
BssHII G'CGCG_C
Cuts at: 0 613 819 2076
Size: 613 206 1257
Fragments arranged by size:
1257 613 206
BssSI C'ACGA_G
Cuts at: 0 490 2076
Size: 490 1586
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1410 1562 2076
Size: 1410 152 514
Fragments arranged by size:
1410 514 152
BstXI CCAn_nnnn'nTGG
Cuts at: 0 729 1935 2076
Size: 729 1206 141
Fragments arranged by size:
1206 729 141
BstYI r'GATC_y
Cuts at: 0 494 546 2062 2076
Size: 494 52 1516 14
Fragments arranged by size:
1516 494 52 14
BtgI C'CryG_G
Cuts at: 0 1014 1073 1617 1928 2076
Size: 1014 59 544 311 148
Fragments arranged by size:
1014 544 311 148 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1854 2076
Size: 1854 222
BtsI GCAGTG_nn'
Cuts at: 0 1176 1219 1375 1881 2076
Size: 1176 43 156 506 195
Fragments arranged by size:
1176 506 195 156 43
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1606 1641 2076
Size: 1606 35 435
Fragments arranged by size:
1606 435 35
DraIII CAC_nnn'GTG
Cuts at: 0 1159 1410 1529 2076
Size: 1159 251 119 547
Fragments arranged by size:
1159 547 251 119
EaeI y'GGCC_r
Cuts at: 0 67 457 537 945 1305 1440 1751 2076
Size: 67 390 80 408 360 135 311 325
Fragments arranged by size:
408 390 360 325 311 135 80 67
EcoICRI GAG'CTC
Cuts at: 0 22 228 2076
Size: 22 206 1848
Fragments arranged by size:
1848 206 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 187 229 236 262 799 1063 1073
Size: 27 160 42 7 26 537 264 10
Cuts at: 1073 1225 1573 1640 2076
Size: 152 348 67 436
Fragments arranged by size:
537 436 348 264 160 152 67 42
27 26 10 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1161 2076
Size: 1161 915
EcoO109I rG'GnC_Cy
Cuts at: 0 11 29 132 620 1001 1231 1863 1939
Size: 11 18 103 488 381 230 632 76
Cuts at: 1939 2076
Size: 137
Fragments arranged by size:
632 488 381 230 137 103 76 18
11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1593 1707 2076
Size: 1593 114 369
Fragments arranged by size:
1593 369 114
FseI GG_CCGG'CC
Cuts at: 0 1796 2076
Size: 1796 280
HaeII r_GCGC'y
Cuts at: 0 83 769 848 895 1060 1563 1596 2076
Size: 83 686 79 47 165 503 33 480
Fragments arranged by size:
686 503 480 165 83 79 47 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2054 2076
Size: 2054 22
KasI G'GCGC_C
Cuts at: 0 79 2076
Size: 79 1997
MscI TGG'CCA
Cuts at: 0 69 459 539 947 1442 1753 2076
Size: 69 390 80 408 495 311 323
Fragments arranged by size:
495 408 390 323 311 80 69
MslI CAynn'nnrTG
Cuts at: 0 760 1436 2076
Size: 760 676 640
Fragments arranged by size:
760 676 640
MspA1I CmG'CkG
Cuts at: 0 513 816 1488 1847 2076
Size: 513 303 672 359 229
Fragments arranged by size:
672 513 359 303 229
NaeI GCC'GGC
Cuts at: 0 890 1049 1794 2076
Size: 890 159 745 282
Fragments arranged by size:
890 745 282 159
NarI GG'CG_CC
Cuts at: 0 80 2076
Size: 80 1996
NcoI C'CATG_G
Cuts at: 0 1073 1928 2076
Size: 1073 855 148
Fragments arranged by size:
1073 855 148
NgoMIV G'CCGG_C
Cuts at: 0 888 1047 1792 2076
Size: 888 159 745 284
Fragments arranged by size:
888 745 284 159
NspI r_CATG'y
Cuts at: 0 1196 2076
Size: 1196 880
PasI CC'CwG_GG
Cuts at: 0 106 1786 1911 2076
Size: 106 1680 125 165
Fragments arranged by size:
1680 165 125 106
PflMI CCAn_nnn'nTGG
Cuts at: 0 1074 1693 1911 2076
Size: 1074 619 218 165
Fragments arranged by size:
1074 619 218 165
PfoI T'CCnGG_A
Cuts at: 0 134 710 1580 2076
Size: 134 576 870 496
Fragments arranged by size:
870 576 496 134
PpuMI rG'GwC_Cy
Cuts at: 0 29 132 620 1863 2076
Size: 29 103 488 1243 213
Fragments arranged by size:
1243 488 213 103 29
PspOMI G'GGCC_C
Cuts at: 0 1001 1938 2076
Size: 1001 937 138
Fragments arranged by size:
1001 937 138
PstI C_TGCA'G
Cuts at: 0 1370 1567 1838 1858 2076
Size: 1370 197 271 20 218
Fragments arranged by size:
1370 271 218 197 20
PvuII CAG'CTG
Cuts at: 0 513 816 1488 1847 2076
Size: 513 303 672 359 229
Fragments arranged by size:
672 513 359 303 229
SacI G_AGCT'C
Cuts at: 0 24 230 2076
Size: 24 206 1846
Fragments arranged by size:
1846 206 24
SanDI GG'GwC_CC
Cuts at: 0 29 1863 2076
Size: 29 1834 213
Fragments arranged by size:
1834 213 29
SfcI C'TryA_G
Cuts at: 0 1366 1563 1834 1854 2076
Size: 1366 197 271 20 222
Fragments arranged by size:
1366 271 222 197 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1798 2076
Size: 1798 278
SfoI GGC'GCC
Cuts at: 0 81 2076
Size: 81 1995
SgrAI Cr'CCGG_yG
Cuts at: 0 888 2076
Size: 888 1188
SmaI CCC'GGG
Cuts at: 0 466 998 2076
Size: 466 532 1078
Fragments arranged by size:
1078 532 466
SmlI C'TyrA_G
Cuts at: 0 235 2076
Size: 235 1841
StuI AGG'CCT
Cuts at: 0 1258 2076
Size: 1258 818
StyI C'CwwG_G
Cuts at: 0 348 473 1073 1466 1928 2076
Size: 348 125 600 393 462 148
Fragments arranged by size:
600 462 393 348 148 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 805 1066 1259 1540 2076
Size: 805 261 193 281 536
Fragments arranged by size:
805 536 281 261 193
TatI w'GTAC_w
Cuts at: 0 641 2076
Size: 641 1435
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 866 898 1198 1230 1339 1371 1855 1887
Size: 866 32 300 32 109 32 484 32
Cuts at: 1887 2076
Size: 189
Fragments arranged by size:
866 484 300 189 109 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 547 1485 2076
Size: 547 938 591
Fragments arranged by size:
938 591 547
XmaI C'CCGG_G
Cuts at: 0 464 996 2076
Size: 464 532 1080
Fragments arranged by size:
1080 532 464
XmnI GAAnn'nnTTC
Cuts at: 0 907 1576 2076
Size: 907 669 500
Fragments arranged by size:
907 669 500
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII
EaeI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII
Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SanDI SfcI SfiI SfoI SgrAI SmaI
SmlI StuI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbsI BcgI BfrBI BlpI BmgBI BmtI
BsaBI BsaWI BsiEI BsiWI BspEI BstBI BstEII BstZ17I
Bsu36I ClaI DraI DrdI EagI EarI EciI EcoRI
EcoRV FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SgfI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI ZraI