Restriction map for
pFN21AE5744
(Linear) (Six-base) MAPSORT of: KIEE5744.seq Check: 5656 from: 1 to: 1878
KIEE5744.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1191 1512 1878
Size: 1191 321 366
Fragments arranged by size:
1191 366 321
AclI AA'CG_TT
Cuts at: 0 1357 1878
Size: 1357 521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1150 1759 1878
Size: 1006 144 609 119
Fragments arranged by size:
1006 609 144 119
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1171 1203 1878
Size: 1171 32 675
Fragments arranged by size:
1171 675 32
AlwNI CAG_nnn'CTG
Cuts at: 0 258 512 705 1206 1491 1878
Size: 258 254 193 501 285 387
Fragments arranged by size:
501 387 285 258 254 193
ApaLI G'TGCA_C
Cuts at: 0 580 1878
Size: 580 1298
ApoI r'AATT_y
Cuts at: 0 891 1783 1870 1878
Size: 891 892 87 8
Fragments arranged by size:
892 891 87 8
AvaI C'yCGr_G
Cuts at: 0 34 796 1439 1475 1562 1878
Size: 34 762 643 36 87 316
Fragments arranged by size:
762 643 316 87 36 34
BanI G'GyrC_C
Cuts at: 0 764 1878
Size: 764 1114
BanII G_rGCy'C
Cuts at: 0 215 272 473 791 821 1878
Size: 215 57 201 318 30 1057
Fragments arranged by size:
1057 318 215 201 57 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 244 920 1424 1878
Size: 244 676 504 454
Fragments arranged by size:
676 504 454 244
BbvCI CC'TCA_GC
Cuts at: 0 1156 1588 1878
Size: 1156 432 290
Fragments arranged by size:
1156 432 290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 84 118 411 445 532 566 1878
Size: 84 34 293 34 87 34 1312
Fragments arranged by size:
1312 293 87 84 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1057 1878
Size: 1057 821
BglI GCCn_nnn'nGGC
Cuts at: 0 562 1878
Size: 562 1316
Bme1580I G_kGCm'C
Cuts at: 0 584 1878
Size: 584 1294
BmgBI CAC'GTC
Cuts at: 0 1221 1878
Size: 1221 657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 487 1228 1261 1498 1878
Size: 187 300 741 33 237 380
Fragments arranged by size:
741 380 300 237 187 33
Bpu10I CC'TnA_GC
Cuts at: 0 1156 1588 1878
Size: 1156 432 290
Fragments arranged by size:
1156 432 290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 457 1878
Size: 199 258 1421
Fragments arranged by size:
1421 258 199
BsaI GGTCTCn'nnnn_
Cuts at: 0 964 1878
Size: 964 914
BsaHI Gr'CG_yC
Cuts at: 0 827 1123 1757 1878
Size: 827 296 634 121
Fragments arranged by size:
827 634 296 121
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1171 1201 1878
Size: 1171 30 677
Fragments arranged by size:
1171 677 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 247 427 511 1075 1351 1459 1878
Size: 85 162 180 84 564 276 108 419
Fragments arranged by size:
564 419 276 180 162 108 85 84
BseYI C'CCAG_C
Cuts at: 0 688 1401 1878
Size: 688 713 477
Fragments arranged by size:
713 688 477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 703 1878
Size: 76 627 1175
Fragments arranged by size:
1175 627 76
BsiEI CG_ry'CG
Cuts at: 0 374 1878
Size: 374 1504
BsiHKAI G_wGCw'C
Cuts at: 0 215 473 584 791 821 1878
Size: 215 258 111 207 30 1057
Fragments arranged by size:
1057 258 215 207 111 30
BsmI GAATG_Cn'
Cuts at: 0 95 1878
Size: 95 1783
BsmBI CGTCTCn'nnnn_
Cuts at: 0 37 1130 1878
Size: 37 1093 748
Fragments arranged by size:
1093 748 37
Bsp1286I G_dGCh'C
Cuts at: 0 215 272 473 584 791 821 1878
Size: 215 57 201 111 207 30 1057
Fragments arranged by size:
1057 215 207 201 111 57 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 554 1191 1463 1512 1878
Size: 554 637 272 49 366
Fragments arranged by size:
637 554 366 272 49
BsrBI CCG'CTC
Cuts at: 0 147 762 1878
Size: 147 615 1116
Fragments arranged by size:
1116 615 147
BssHII G'CGCG_C
Cuts at: 0 298 406 418 481 1878
Size: 298 108 12 63 1397
Fragments arranged by size:
1397 298 108 63 12
BssSI C'ACGA_G
Cuts at: 0 283 965 1878
Size: 283 682 913
Fragments arranged by size:
913 682 283
BstAPI GCAn_nnn'nTGC
Cuts at: 0 258 1878
Size: 258 1620
BstYI r'GATC_y
Cuts at: 0 461 1032 1878
Size: 461 571 846
Fragments arranged by size:
846 571 461
Bsu36I CC'TnA_GG
Cuts at: 0 1192 1878
Size: 1192 686
EaeI y'GGCC_r
Cuts at: 0 62 371 1878
Size: 62 309 1507
Fragments arranged by size:
1507 309 62
EagI C'GGCC_G
Cuts at: 0 371 1878
Size: 371 1507
EarI CTCTTCn'nnn_
Cuts at: 0 1655 1721 1878
Size: 1655 66 157
Fragments arranged by size:
1655 157 66
EcoICRI GAG'CTC
Cuts at: 0 213 471 789 819 1878
Size: 213 258 318 30 1059
Fragments arranged by size:
1059 318 258 213 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 487 1006 1150 1228 1261 1498 1759
Size: 187 300 519 144 78 33 237 261
Cuts at: 1759 1878
Size: 119
Fragments arranged by size:
519 300 261 237 187 144 119 78
33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1513 1878
Size: 1513 365
EcoO109I rG'GnC_Cy
Cuts at: 0 425 1397 1488 1878
Size: 425 972 91 390
Fragments arranged by size:
972 425 390 91
EcoRI G'AATT_C
Cuts at: 0 891 1783 1878
Size: 891 892 95
Fragments arranged by size:
892 891 95
HaeII r_GCGC'y
Cuts at: 0 259 394 444 505 1878
Size: 259 135 50 61 1373
Fragments arranged by size:
1373 259 135 61 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1219 1251 1324 1356 1802 1834 1878
Size: 1219 32 73 32 446 32 44
Fragments arranged by size:
1219 446 73 44 32 32 32
MscI TGG'CCA
Cuts at: 0 64 1878
Size: 64 1814
MspA1I CmG'CkG
Cuts at: 0 333 738 984 1470 1671 1737 1878
Size: 333 405 246 486 201 66 141
Fragments arranged by size:
486 405 333 246 201 141 66
NdeI CA'TA_TG
Cuts at: 0 842 1878
Size: 842 1036
NspI r_CATG'y
Cuts at: 0 519 1309 1878
Size: 519 790 569
Fragments arranged by size:
790 569 519
PflMI CCAn_nnn'nTGG
Cuts at: 0 1056 1878
Size: 1056 822
PfoI T'CCnGG_A
Cuts at: 0 464 830 1514 1878
Size: 464 366 684 364
Fragments arranged by size:
684 464 366 364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1171 1203 1878
Size: 1171 32 675
Fragments arranged by size:
1171 675 32
PpuMI rG'GwC_Cy
Cuts at: 0 1397 1878
Size: 1397 481
PstI C_TGCA'G
Cuts at: 0 155 254 332 455 716 1202 1878
Size: 155 99 78 123 261 486 676
Fragments arranged by size:
676 486 261 155 123 99 78
PvuII CAG'CTG
Cuts at: 0 738 984 1470 1671 1737 1878
Size: 738 246 486 201 66 141
Fragments arranged by size:
738 486 246 201 141 66
SacI G_AGCT'C
Cuts at: 0 215 473 791 821 1878
Size: 215 258 318 30 1057
Fragments arranged by size:
1057 318 258 215 30
SfcI C'TryA_G
Cuts at: 0 151 250 328 337 451 712 1198 1878
Size: 151 99 78 9 114 261 486 680
Fragments arranged by size:
680 486 261 151 114 99 78 9
SmaI CCC'GGG
Cuts at: 0 798 1477 1878
Size: 798 679 401
Fragments arranged by size:
798 679 401
SmlI C'TyrA_G
Cuts at: 0 214 472 1878
Size: 214 258 1406
Fragments arranged by size:
1406 258 214
SphI G_CATG'C
Cuts at: 0 519 1878
Size: 519 1359
StyI C'CwwG_G
Cuts at: 0 242 533 662 1760 1878
Size: 242 291 129 1098 118
Fragments arranged by size:
1098 291 242 129 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 988 1878
Size: 415 573 890
Fragments arranged by size:
890 573 415
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 633 665 1636 1668 1878
Size: 633 32 971 32 210
Fragments arranged by size:
971 633 210 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 379 1878
Size: 379 1499
XmaI C'CCGG_G
Cuts at: 0 796 1475 1878
Size: 796 679 403
Fragments arranged by size:
796 679 403
Enzymes that do cut:
AarI AclI AcuI AloI AlwNI ApaLI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BciVI BglI Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BssHII BssSI BstAPI BstYI Bsu36I EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
MscI MspA1I NdeI NspI PflMI PfoI PpiI PpuMI
PstI PvuII SacI SfcI SmaI SmlI SphI StyI
TaqII TstI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BclI BfrBI BglII BlpI BmrI BmtI
BplI BsaAI BsaBI BsaWI BsiWI BspEI BspHI BsrDI
BsrFI BsrGI BstBI BstEII BstXI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EciI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MslI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI TatI XbaI XcmI XhoI XmnI ZraI