Restriction map for
pFN21AE5742
(Linear) (Six-base) MAPSORT of: KIEE5742.seq Check: 2106 from: 1 to: 2034
KIEE5742.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 771 2034
Size: 771 1263
AccI GT'mk_AC
Cuts at: 0 422 1433 2034
Size: 422 1011 601
Fragments arranged by size:
1011 601 422
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 805 1472 1489 2034
Size: 718 87 667 17 545
Fragments arranged by size:
718 667 545 87 17
AflIII A'CryG_T
Cuts at: 0 578 1436 2034
Size: 578 858 598
Fragments arranged by size:
858 598 578
AleI CACnn'nnGTG
Cuts at: 0 1315 2034
Size: 1315 719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 581 1720 1930 2018 2034
Size: 581 1139 210 88 16
Fragments arranged by size:
1139 581 210 88 16
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 443 475 2034
Size: 443 32 1559
Fragments arranged by size:
1559 443 32
AlwNI CAG_nnn'CTG
Cuts at: 0 489 514 960 1176 1206 1522 1841 2034
Size: 489 25 446 216 30 316 319 193
Fragments arranged by size:
489 446 319 316 216 193 30 25
ApaI G_GGCC'C
Cuts at: 0 1200 2034
Size: 1200 834
ApaLI G'TGCA_C
Cuts at: 0 169 2034
Size: 169 1865
ApoI r'AATT_y
Cuts at: 0 754 2034
Size: 754 1280
AvaI C'yCGr_G
Cuts at: 0 92 340 1103 1415 2034
Size: 92 248 763 312 619
Fragments arranged by size:
763 619 312 248 92
BamHI G'GATC_C
Cuts at: 0 678 1776 1937 2034
Size: 678 1098 161 97
Fragments arranged by size:
1098 678 161 97
BanI G'GyrC_C
Cuts at: 0 79 119 284 1720 2034
Size: 79 40 165 1436 314
Fragments arranged by size:
1436 314 165 79 40
BanII G_rGCy'C
Cuts at: 0 365 1200 1373 2034
Size: 365 835 173 661
Fragments arranged by size:
835 661 365 173
BbsI GAAGACnn'nnnn_
Cuts at: 0 94 363 1791 2034
Size: 94 269 1428 243
Fragments arranged by size:
1428 269 243 94
BclI T'GATC_A
Cuts at: 0 29 1481 2034
Size: 29 1452 553
Fragments arranged by size:
1452 553 29
BglI GCCn_nnn'nGGC
Cuts at: 0 1021 2034
Size: 1021 1013
Bme1580I G_kGCm'C
Cuts at: 0 173 471 1200 1416 1723 1967 2034
Size: 173 298 729 216 307 244 67
Fragments arranged by size:
729 307 298 244 216 173 67
BmgBI CAC'GTC
Cuts at: 0 552 942 2034
Size: 552 390 1092
Fragments arranged by size:
1092 552 390
BmrI ACTGGGnnnn_n'
Cuts at: 0 1636 2034
Size: 1636 398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 445 751 1072 1144 1516 1755 1939
Size: 64 381 306 321 72 372 239 184
Cuts at: 1939 2034
Size: 95
Fragments arranged by size:
381 372 321 306 239 184 95 72
64
BsaI GGTCTCn'nnnn_
Cuts at: 0 499 1915 2034
Size: 499 1416 119
Fragments arranged by size:
1416 499 119
BsaAI yAC'GTr
Cuts at: 0 259 357 581 1653 2034
Size: 259 98 224 1072 381
Fragments arranged by size:
1072 381 259 224 98
BsaBI GATnn'nnATC
Cuts at: 0 926 2034
Size: 926 1108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 131 161 443 473 1130 1160 2034
Size: 25 106 30 282 30 657 30 874
Fragments arranged by size:
874 657 282 106 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 361 364 1339 2034
Size: 358 3 3 975 695
Fragments arranged by size:
975 695 358 3 3
BseYI C'CCAG_C
Cuts at: 0 182 489 614 814 1199 1216 1613 2034
Size: 182 307 125 200 385 17 397 421
Fragments arranged by size:
421 397 385 307 200 182 125 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 1549 1675 2034
Size: 288 1261 126 359
Fragments arranged by size:
1261 359 288 126
BsiHKAI G_wGCw'C
Cuts at: 0 173 356 365 629 941 1237 1652 1898
Size: 173 183 9 264 312 296 415 246
Cuts at: 1898 2034
Size: 136
Fragments arranged by size:
415 312 296 264 246 183 173 136
9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1492 2034
Size: 1492 542
Bsp1286I G_dGCh'C
Cuts at: 0 173 356 365 471 629 941 1200 1237
Size: 173 183 9 106 158 312 259 37
Cuts at: 1237 1373 1416 1652 1723 1898 1967 2034
Size: 136 43 236 71 175 69 67
Fragments arranged by size:
312 259 236 183 175 173 158 136
106 71 69 67 43 37 9
BspHI T'CATG_A
Cuts at: 0 434 2034
Size: 434 1600
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 302 771 1712 2034
Size: 302 469 941 322
Fragments arranged by size:
941 469 322 302
BsrDI GCAATG_nn'
Cuts at: 0 1344 1356 2034
Size: 1344 12 678
Fragments arranged by size:
1344 678 12
BsrFI r'CCGG_y
Cuts at: 0 974 2034
Size: 974 1060
BssSI C'ACGA_G
Cuts at: 0 1311 2034
Size: 1311 723
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1176 1522 1673 2034
Size: 1176 346 151 361
Fragments arranged by size:
1176 361 346 151
BstEII G'GTnAC_C
Cuts at: 0 177 2034
Size: 177 1857
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1317 2034
Size: 1317 717
BstYI r'GATC_y
Cuts at: 0 678 956 1067 1562 1569 1776 1937 2034
Size: 678 278 111 495 7 207 161 97
Fragments arranged by size:
678 495 278 207 161 111 97 7
BstZ17I GTA'TAC
Cuts at: 0 1434 2034
Size: 1434 600
Bsu36I CC'TnA_GG
Cuts at: 0 850 1928 2034
Size: 850 1078 106
Fragments arranged by size:
1078 850 106
BtgI C'CryG_G
Cuts at: 0 74 896 1391 1692 2034
Size: 74 822 495 301 342
Fragments arranged by size:
822 495 342 301 74
BtsI GCAGTG_nn'
Cuts at: 0 1523 2034
Size: 1523 511
DraIII CAC_nnn'GTG
Cuts at: 0 177 584 2034
Size: 177 407 1450
Fragments arranged by size:
1450 407 177
EaeI y'GGCC_r
Cuts at: 0 110 270 1307 2034
Size: 110 160 1037 727
Fragments arranged by size:
1037 727 160 110
EarI CTCTTCn'nnn_
Cuts at: 0 188 2034
Size: 188 1846
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1336 1950 2034
Size: 1336 614 84
Fragments arranged by size:
1336 614 84
EcoICRI GAG'CTC
Cuts at: 0 363 2034
Size: 363 1671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 445 718 751 805 1072 1144 1472
Size: 64 381 273 33 54 267 72 328
Cuts at: 1472 1489 1516 1755 1939 2034
Size: 17 27 239 184 95
Fragments arranged by size:
381 328 273 267 239 184 95 72
64 54 33 27 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1708 2034
Size: 1708 326
EcoO109I rG'GnC_Cy
Cuts at: 0 521 1173 2034
Size: 521 652 861
Fragments arranged by size:
861 652 521
HaeII r_GCGC'y
Cuts at: 0 858 975 1495 2034
Size: 858 117 520 539
Fragments arranged by size:
858 539 520 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1554 1586 1999 2031 2034
Size: 27 1527 32 413 32 3
Fragments arranged by size:
1527 413 32 32 27 3
HincII GTy'rAC
Cuts at: 0 162 2034
Size: 162 1872
MluI A'CGCG_T
Cuts at: 0 1436 2034
Size: 1436 598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1997 2034
Size: 1997 37
MscI TGG'CCA
Cuts at: 0 112 272 1309 2034
Size: 112 160 1037 725
Fragments arranged by size:
1037 725 160 112
MslI CAynn'nnrTG
Cuts at: 0 1240 1315 1349 1733 2034
Size: 1240 75 34 384 301
Fragments arranged by size:
1240 384 301 75 34
MspA1I CmG'CkG
Cuts at: 0 618 1617 1629 1832 2034
Size: 618 999 12 203 202
Fragments arranged by size:
999 618 203 202 12
NaeI GCC'GGC
Cuts at: 0 976 2034
Size: 976 1058
NcoI C'CATG_G
Cuts at: 0 1391 1692 2034
Size: 1391 301 342
Fragments arranged by size:
1391 342 301
NgoMIV G'CCGG_C
Cuts at: 0 974 2034
Size: 974 1060
NspI r_CATG'y
Cuts at: 0 210 1914 2034
Size: 210 1704 120
Fragments arranged by size:
1704 210 120
PflMI CCAn_nnn'nTGG
Cuts at: 0 262 489 514 1952 2034
Size: 262 227 25 1438 82
Fragments arranged by size:
1438 262 227 82 25
PfoI T'CCnGG_A
Cuts at: 0 1565 2034
Size: 1565 469
PmlI CAC'GTG
Cuts at: 0 259 357 581 1653 2034
Size: 259 98 224 1072 381
Fragments arranged by size:
1072 381 259 224 98
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 443 475 2034
Size: 443 32 1559
Fragments arranged by size:
1559 443 32
PspOMI G'GGCC_C
Cuts at: 0 1196 2034
Size: 1196 838
PstI C_TGCA'G
Cuts at: 0 323 560 623 920 1181 1577 1837 1865
Size: 323 237 63 297 261 396 260 28
Cuts at: 1865 1997 2034
Size: 132 37
Fragments arranged by size:
396 323 297 261 260 237 132 63
37 28
PvuII CAG'CTG
Cuts at: 0 618 1832 2034
Size: 618 1214 202
Fragments arranged by size:
1214 618 202
SacI G_AGCT'C
Cuts at: 0 365 2034
Size: 365 1669
SexAI A'CCwGG_T
Cuts at: 0 173 2034
Size: 173 1861
SfcI C'TryA_G
Cuts at: 0 319 556 619 916 1177 1573 1833 1861
Size: 319 237 63 297 261 396 260 28
Cuts at: 1861 1993 2034
Size: 132 41
Fragments arranged by size:
396 319 297 261 260 237 132 63
41 28
SmaI CCC'GGG
Cuts at: 0 94 1417 2034
Size: 94 1323 617
Fragments arranged by size:
1323 617 94
SphI G_CATG'C
Cuts at: 0 210 1914 2034
Size: 210 1704 120
Fragments arranged by size:
1704 210 120
SrfI GCCC'GGGC
Cuts at: 0 1417 2034
Size: 1417 617
StyI C'CwwG_G
Cuts at: 0 143 905 1391 1692 2034
Size: 143 762 486 301 342
Fragments arranged by size:
762 486 342 301 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1252 1738 2034
Size: 1252 486 296
Fragments arranged by size:
1252 486 296
TatI w'GTAC_w
Cuts at: 0 650 2034
Size: 650 1384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 131 163 568 600 1130 1162 2034
Size: 131 32 405 32 530 32 872
Fragments arranged by size:
872 530 405 131 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 418 886 2034
Size: 418 468 1148
Fragments arranged by size:
1148 468 418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 682 1207 1779 2034
Size: 682 525 572 255
Fragments arranged by size:
682 572 525 255
XmaI C'CCGG_G
Cuts at: 0 92 1415 2034
Size: 92 1323 619
Fragments arranged by size:
1323 619 92
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BclI BglI Bme1580I BmgBI BmrI BpmI BsaI BsaAI
BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BssSI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
MluI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PflMI PfoI PmlI PpiI PspOMI PstI PvuII
SacI SexAI SfcI SmaI SphI SrfI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI
BfrBI BglII BlpI BmtI BplI Bpu10I BpuEI BsaHI
BsaWI BsiEI BsiWI BsmI BspEI BsrBI BsrGI BssHII
BstBI BtgZI ClaI CspCI DraI DrdI EagI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SspI StuI SwaI XbaI XhoI XmnI ZraI