Restriction map for
pFN21AE5741
(Linear) (Six-base) MAPSORT of: KIEE5741.seq Check: 6871 from: 1 to: 1869
KIEE5741.orf
With 182 enzymes: SgfI *
December 5, 2014 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 870 1869
Size: 870 999
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 151 415 671 1329 1869
Size: 31 120 264 256 658 540
Fragments arranged by size:
658 540 264 256 120 31
AflII C'TTAA_G
Cuts at: 0 504 1869
Size: 504 1365
AhdI GACnn_n'nnGTC
Cuts at: 0 714 1869
Size: 714 1155
AlwNI CAG_nnn'CTG
Cuts at: 0 420 1869
Size: 420 1449
ApoI r'AATT_y
Cuts at: 0 1210 1310 1869
Size: 1210 100 559
Fragments arranged by size:
1210 559 100
BanII G_rGCy'C
Cuts at: 0 971 1869
Size: 971 898
BbsI GAAGACnn'nnnn_
Cuts at: 0 363 569 677 919 1658 1778 1829 1869
Size: 363 206 108 242 739 120 51 40
Fragments arranged by size:
739 363 242 206 120 108 51 40
BbvCI CC'TCA_GC
Cuts at: 0 26 68 1715 1869
Size: 26 42 1647 154
Fragments arranged by size:
1647 154 42 26
BclI T'GATC_A
Cuts at: 0 17 1869
Size: 17 1852
BfrBI ATG'CAT
Cuts at: 0 1621 1869
Size: 1621 248
BglII A'GATC_T
Cuts at: 0 1860 1869
Size: 1860 9
BlpI GC'TnA_GC
Cuts at: 0 424 1869
Size: 424 1445
Bme1580I G_kGCm'C
Cuts at: 0 1133 1869
Size: 1133 736
BmgBI CAC'GTC
Cuts at: 0 1740 1869
Size: 1740 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 740 1075 1869
Size: 740 335 794
Fragments arranged by size:
794 740 335
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1070 1869
Size: 1070 799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 388 566 1869
Size: 373 15 178 1303
Fragments arranged by size:
1303 373 178 15
Bpu10I CC'TnA_GC
Cuts at: 0 26 68 1715 1869
Size: 26 42 1647 154
Fragments arranged by size:
1647 154 42 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 1869
Size: 479 1390
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 595 625 1869
Size: 595 30 1244
Fragments arranged by size:
1244 595 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 56 603 615 739 1869
Size: 56 547 12 124 1130
Fragments arranged by size:
1130 547 124 56 12
BseYI C'CCAG_C
Cuts at: 0 359 1119 1869
Size: 359 760 750
Fragments arranged by size:
760 750 359
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1381 1869
Size: 1381 488
BsmI GAATG_Cn'
Cuts at: 0 1377 1869
Size: 1377 492
Bsp1286I G_dGCh'C
Cuts at: 0 971 1133 1869
Size: 971 162 736
Fragments arranged by size:
971 736 162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 870 1869
Size: 870 999
BsrDI GCAATG_nn'
Cuts at: 0 941 1692 1869
Size: 941 751 177
Fragments arranged by size:
941 751 177
BstAPI GCAn_nnn'nTGC
Cuts at: 0 420 885 1869
Size: 420 465 984
Fragments arranged by size:
984 465 420
BstYI r'GATC_y
Cuts at: 0 41 1860 1869
Size: 41 1819 9
Fragments arranged by size:
1819 41 9
BtgI C'CryG_G
Cuts at: 0 756 1869
Size: 756 1113
BtsI GCAGTG_nn'
Cuts at: 0 854 1869
Size: 854 1015
DraI TTT'AAA
Cuts at: 0 815 1869
Size: 815 1054
EaeI y'GGCC_r
Cuts at: 0 462 753 772 1869
Size: 462 291 19 1097
Fragments arranged by size:
1097 462 291 19
EarI CTCTTCn'nnn_
Cuts at: 0 1742 1838 1869
Size: 1742 96 31
Fragments arranged by size:
1742 96 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 151 373 388 415 566 671 1329
Size: 31 120 222 15 27 151 105 658
Cuts at: 1329 1869
Size: 540
Fragments arranged by size:
658 540 222 151 120 105 31 27
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1636 1869
Size: 1636 233
EcoO109I rG'GnC_Cy
Cuts at: 0 734 1102 1869
Size: 734 368 767
Fragments arranged by size:
767 734 368
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 9 41 700 732 1837 1869
Size: 9 32 659 32 1105 32
Fragments arranged by size:
1105 659 32 32 32 9
HincII GTy'rAC
Cuts at: 0 1298 1869
Size: 1298 571
HpaI GTT'AAC
Cuts at: 0 1298 1869
Size: 1298 571
MfeI C'AATT_G
Cuts at: 0 349 1869
Size: 349 1520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 653 1010 1522 1869
Size: 114 539 357 512 347
Fragments arranged by size:
539 512 357 347 114
MscI TGG'CCA
Cuts at: 0 464 755 774 1869
Size: 464 291 19 1095
Fragments arranged by size:
1095 464 291 19
MslI CAynn'nnrTG
Cuts at: 0 1180 1869
Size: 1180 689
MspA1I CmG'CkG
Cuts at: 0 1319 1869
Size: 1319 550
NcoI C'CATG_G
Cuts at: 0 756 1869
Size: 756 1113
NsiI A_TGCA'T
Cuts at: 0 1623 1869
Size: 1623 246
NspI r_CATG'y
Cuts at: 0 957 1869
Size: 957 912
PasI CC'CwG_GG
Cuts at: 0 1098 1869
Size: 1098 771
PfoI T'CCnGG_A
Cuts at: 0 300 1602 1869
Size: 300 1302 267
Fragments arranged by size:
1302 300 267
PpuMI rG'GwC_Cy
Cuts at: 0 1102 1869
Size: 1102 767
PsiI TTA'TAA
Cuts at: 0 1217 1869
Size: 1217 652
PstI C_TGCA'G
Cuts at: 0 116 1640 1869
Size: 116 1524 229
Fragments arranged by size:
1524 229 116
PvuII CAG'CTG
Cuts at: 0 1319 1869
Size: 1319 550
SanDI GG'GwC_CC
Cuts at: 0 1102 1869
Size: 1102 767
SapI GCTCTTCn'nnn_
Cuts at: 0 1742 1838 1869
Size: 1742 96 31
Fragments arranged by size:
1742 96 31
SfcI C'TryA_G
Cuts at: 0 112 1636 1869
Size: 112 1524 233
Fragments arranged by size:
1524 233 112
SmlI C'TyrA_G
Cuts at: 0 494 504 1869
Size: 494 10 1365
Fragments arranged by size:
1365 494 10
StyI C'CwwG_G
Cuts at: 0 756 1568 1869
Size: 756 812 301
Fragments arranged by size:
812 756 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 863 1869
Size: 863 1006
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 464 496 1869
Size: 464 32 1373
Fragments arranged by size:
1373 464 32
XmnI GAAnn'nnTTC
Cuts at: 0 926 1869
Size: 926 943
Enzymes that do cut:
AarI AcuI AflII AhdI AlwNI ApoI BanII BbsI
BbvCI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI
BsmI Bsp1286I BspMI BsrDI BstAPI BstYI BtgI BtsI
DraI EaeI EarI Eco57MI EcoNI EcoO109I Hin4I HincII
HpaI MfeI MmeI MscI MslI MspA1I NcoI NsiI
NspI PasI PfoI PpuMI PsiI PstI PvuII SanDI
SapI SfcI SmlI StyI TaqII TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BglI
BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PciI PflMI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI ZraI