Restriction map for
pFN21AE5737
(Linear) (Six-base) MAPSORT of: KIEE5737.seq Check: 399 from: 1 to: 2280
KIEE5737.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AatII G_ACGT'C
Cuts at: 0 1796 2280
Size: 1796 484
Acc65I G'GTAC_C
Cuts at: 0 1739 2280
Size: 1739 541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 696 883 1482 2092 2280
Size: 313 383 187 599 610 188
Fragments arranged by size:
610 599 383 313 188 187
AleI CACnn'nnGTG
Cuts at: 0 631 2280
Size: 631 1649
AlwNI CAG_nnn'CTG
Cuts at: 0 781 1505 2175 2280
Size: 781 724 670 105
Fragments arranged by size:
781 724 670 105
ApaLI G'TGCA_C
Cuts at: 0 682 806 2280
Size: 682 124 1474
Fragments arranged by size:
1474 682 124
AvaI C'yCGr_G
Cuts at: 0 2041 2280
Size: 2041 239
BanI G'GyrC_C
Cuts at: 0 244 433 1739 2280
Size: 244 189 1306 541
Fragments arranged by size:
1306 541 244 189
BanII G_rGCy'C
Cuts at: 0 1190 1221 2280
Size: 1190 31 1059
Fragments arranged by size:
1190 1059 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 871 1011 1220 2280
Size: 871 140 209 1060
Fragments arranged by size:
1060 871 209 140
BbvCI CC'TCA_GC
Cuts at: 0 2095 2280
Size: 2095 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1836 1870 2280
Size: 1836 34 410
Fragments arranged by size:
1836 410 34
BfrBI ATG'CAT
Cuts at: 0 1693 2280
Size: 1693 587
BglI GCCn_nnn'nGGC
Cuts at: 0 898 2245 2280
Size: 898 1347 35
Fragments arranged by size:
1347 898 35
Bme1580I G_kGCm'C
Cuts at: 0 247 686 810 1652 2280
Size: 247 439 124 842 628
Fragments arranged by size:
842 628 439 247 124
BmgBI CAC'GTC
Cuts at: 0 1755 1863 2280
Size: 1755 108 417
Fragments arranged by size:
1755 417 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 2167 2280
Size: 2167 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 1132 1657 1899 2280
Size: 806 326 525 242 381
Fragments arranged by size:
806 525 381 326 242
Bpu10I CC'TnA_GC
Cuts at: 0 106 2095 2280
Size: 106 1989 185
Fragments arranged by size:
1989 185 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 2280
Size: 1243 1037
BsaI GGTCTCn'nnnn_
Cuts at: 0 352 1876 1917 2280
Size: 352 1524 41 363
Fragments arranged by size:
1524 363 352 41
BsaAI yAC'GTr
Cuts at: 0 687 2280
Size: 687 1593
BsaHI Gr'CG_yC
Cuts at: 0 1793 2280
Size: 1793 487
BsaWI w'CCGG_w
Cuts at: 0 649 2150 2280
Size: 649 1501 130
Fragments arranged by size:
1501 649 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 779 809 2280
Size: 779 30 1471
Fragments arranged by size:
1471 779 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1965 2280
Size: 1965 315
BseYI C'CCAG_C
Cuts at: 0 253 1092 1232 1411 2280
Size: 253 839 140 179 869
Fragments arranged by size:
869 839 253 179 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1089 1530 1541 2280
Size: 1089 441 11 739
Fragments arranged by size:
1089 739 441 11
BsiEI CG_ry'CG
Cuts at: 0 1297 2280
Size: 1297 983
BsiHKAI G_wGCw'C
Cuts at: 0 686 810 1180 1190 1835 2280
Size: 686 124 370 10 645 445
Fragments arranged by size:
686 645 445 370 124 10
BsmBI CGTCTCn'nnnn_
Cuts at: 0 949 1861 2280
Size: 949 912 419
Fragments arranged by size:
949 912 419
Bsp1286I G_dGCh'C
Cuts at: 0 247 686 810 1180 1190 1221 1652 1835
Size: 247 439 124 370 10 31 431 183
Cuts at: 1835 2280
Size: 445
Fragments arranged by size:
445 439 431 370 247 183 124 31
10
BspEI T'CCGG_A
Cuts at: 0 2150 2280
Size: 2150 130
BsrBI CCG'CTC
Cuts at: 0 572 2189 2280
Size: 572 1617 91
Fragments arranged by size:
1617 572 91
BsrDI GCAATG_nn'
Cuts at: 0 1145 1556 2280
Size: 1145 411 724
Fragments arranged by size:
1145 724 411
BsrFI r'CCGG_y
Cuts at: 0 1292 1296 2049 2280
Size: 1292 4 753 231
Fragments arranged by size:
1292 753 231 4
BsrGI T'GTAC_A
Cuts at: 0 464 2280
Size: 464 1816
BstAPI GCAn_nnn'nTGC
Cuts at: 0 529 781 2280
Size: 529 252 1499
Fragments arranged by size:
1499 529 252
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1092 1735 2280
Size: 1092 643 545
Fragments arranged by size:
1092 643 545
BstYI r'GATC_y
Cuts at: 0 916 1388 2280
Size: 916 472 892
Fragments arranged by size:
916 892 472
BtgI C'CryG_G
Cuts at: 0 272 1904 2280
Size: 272 1632 376
Fragments arranged by size:
1632 376 272
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 31 1044 2280
Size: 31 1013 1236
Fragments arranged by size:
1236 1013 31
BtsI GCAGTG_nn'
Cuts at: 0 343 1136 2176 2280
Size: 343 793 1040 104
Fragments arranged by size:
1040 793 343 104
ClaI AT'CG_AT
Cuts at: 0 263 2280
Size: 263 2017
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1194 1229 2280
Size: 1194 35 1051
Fragments arranged by size:
1194 1051 35
EaeI y'GGCC_r
Cuts at: 0 1294 1319 1784 2051 2280
Size: 1294 25 465 267 229
Fragments arranged by size:
1294 465 267 229 25
EagI C'GGCC_G
Cuts at: 0 1294 2280
Size: 1294 986
EarI CTCTTCn'nnn_
Cuts at: 0 833 1000 1048 1408 2280
Size: 833 167 48 360 872
Fragments arranged by size:
872 833 360 167 48
EcoICRI GAG'CTC
Cuts at: 0 1188 2280
Size: 1188 1092
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 696 806 883 1132 1482 1657 1899
Size: 313 383 110 77 249 350 175 242
Cuts at: 1899 2092 2280
Size: 193 188
Fragments arranged by size:
383 350 313 249 242 193 188 175
110 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 295 664 873 1068 2280
Size: 295 369 209 195 1212
Fragments arranged by size:
1212 369 295 209 195
EcoO109I rG'GnC_Cy
Cuts at: 0 2256 2280
Size: 2256 24
EcoRV GAT'ATC
Cuts at: 0 2145 2280
Size: 2145 135
HaeII r_GCGC'y
Cuts at: 0 481 1932 2280
Size: 481 1451 348
Fragments arranged by size:
1451 481 348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 2248 2280
Size: 187 32 2029 32
Fragments arranged by size:
2029 187 32 32
KpnI G_GTAC'C
Cuts at: 0 1743 2280
Size: 1743 537
MfeI C'AATT_G
Cuts at: 0 812 2280
Size: 812 1468
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 2280
Size: 386 1894
MscI TGG'CCA
Cuts at: 0 1786 2280
Size: 1786 494
MslI CAynn'nnrTG
Cuts at: 0 631 1123 1321 1420 1481 1733 2125 2280
Size: 631 492 198 99 61 252 392 155
Fragments arranged by size:
631 492 392 252 198 155 99 61
MspA1I CmG'CkG
Cuts at: 0 772 778 1052 1332 1467 2280
Size: 772 6 274 280 135 813
Fragments arranged by size:
813 772 280 274 135 6
NaeI GCC'GGC
Cuts at: 0 1298 2051 2280
Size: 1298 753 229
Fragments arranged by size:
1298 753 229
NdeI CA'TA_TG
Cuts at: 0 169 528 2234 2280
Size: 169 359 1706 46
Fragments arranged by size:
1706 359 169 46
NgoMIV G'CCGG_C
Cuts at: 0 1296 2049 2280
Size: 1296 753 231
Fragments arranged by size:
1296 753 231
NsiI A_TGCA'T
Cuts at: 0 1695 2280
Size: 1695 585
PflMI CCAn_nnn'nTGG
Cuts at: 0 101 1679 2280
Size: 101 1578 601
Fragments arranged by size:
1578 601 101
PmlI CAC'GTG
Cuts at: 0 687 2280
Size: 687 1593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1949 1981 2280
Size: 1949 32 299
Fragments arranged by size:
1949 299 32
PpuMI rG'GwC_Cy
Cuts at: 0 2256 2280
Size: 2256 24
PshAI GACnn'nnGTC
Cuts at: 0 383 2280
Size: 383 1897
PstI C_TGCA'G
Cuts at: 0 777 2280
Size: 777 1503
PvuII CAG'CTG
Cuts at: 0 772 1332 1467 2280
Size: 772 560 135 813
Fragments arranged by size:
813 772 560 135
SacI G_AGCT'C
Cuts at: 0 1190 2280
Size: 1190 1090
SfcI C'TryA_G
Cuts at: 0 773 2280
Size: 773 1507
SmlI C'TyrA_G
Cuts at: 0 1222 2280
Size: 1222 1058
StyI C'CwwG_G
Cuts at: 0 248 660 2280
Size: 248 412 1620
Fragments arranged by size:
1620 412 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 201 1852 1900 2280
Size: 201 1651 48 380
Fragments arranged by size:
1651 380 201 48
TatI w'GTAC_w
Cuts at: 0 464 731 1640 2021 2280
Size: 464 267 909 381 259
Fragments arranged by size:
909 464 381 267 259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 200 232 1442 1474 2280
Size: 200 32 1210 32 806
Fragments arranged by size:
1210 806 200 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1528 1596 2280
Size: 1528 68 684
Fragments arranged by size:
1528 684 68
XbaI T'CTAG_A
Cuts at: 0 834 2280
Size: 834 1446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1329 1419 2280
Size: 1329 90 861
Fragments arranged by size:
1329 861 90
XmnI GAAnn'nnTTC
Cuts at: 0 1961 2280
Size: 1961 319
ZraI GAC'GTC
Cuts at: 0 1794 2280
Size: 1794 486
Enzymes that do cut:
AatII Acc65I AcuI AleI AlwNI ApaLI AvaI BanI
BanII BbsI BbvCI BcgI BfrBI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KpnI
MfeI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV
NsiI PflMI PmlI PpiI PpuMI PshAI PstI PvuII
SacI SfcI SmlI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BclI BglII BlpI BmtI
BplI BsaBI BsiWI BsmI BspHI BspMI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I DraI DraIII DrdI EciI
EcoRI FalI FseI FspI FspAI HincII HindIII HpaI
KasI MluI NarI NcoI NheI NotI NruI NspI
PacI PasI PciI PfoI PmeI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI XhoI XmaI