Restriction map for
pFN21AE5734
(Linear) (Six-base) MAPSORT of: KIEE5734.seq Check: 4037 from: 1 to: 1980
KIEE5734.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AccI GT'mk_AC
Cuts at: 0 552 1980
Size: 552 1428
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 1108 1980
Size: 618 490 872
Fragments arranged by size:
872 618 490
AflIII A'CryG_T
Cuts at: 0 275 1980
Size: 275 1705
AhdI GACnn_n'nnGTC
Cuts at: 0 1869 1980
Size: 1869 111
AleI CACnn'nnGTG
Cuts at: 0 1660 1980
Size: 1660 320
AlwNI CAG_nnn'CTG
Cuts at: 0 624 1980
Size: 624 1356
ApoI r'AATT_y
Cuts at: 0 117 541 1631 1980
Size: 117 424 1090 349
Fragments arranged by size:
1090 424 349 117
AseI AT'TA_AT
Cuts at: 0 1105 1980
Size: 1105 875
BanI G'GyrC_C
Cuts at: 0 644 1980
Size: 644 1336
BanII G_rGCy'C
Cuts at: 0 1383 1685 1780 1980
Size: 1383 302 95 200
Fragments arranged by size:
1383 302 200 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 785 1980
Size: 785 1195
BclI T'GATC_A
Cuts at: 0 1431 1980
Size: 1431 549
BglII A'GATC_T
Cuts at: 0 1845 1980
Size: 1845 135
BlpI GC'TnA_GC
Cuts at: 0 442 1980
Size: 442 1538
Bme1580I G_kGCm'C
Cuts at: 0 642 1980
Size: 642 1338
BmgBI CAC'GTC
Cuts at: 0 276 1980
Size: 276 1704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 931 1172 1980
Size: 652 279 241 808
Fragments arranged by size:
808 652 279 241
Bpu10I CC'TnA_GC
Cuts at: 0 1037 1980
Size: 1037 943
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1333 1980
Size: 1333 647
BsaI GGTCTCn'nnnn_
Cuts at: 0 1904 1980
Size: 1904 76
BsaBI GATnn'nnATC
Cuts at: 0 1844 1980
Size: 1844 136
BsaWI w'CCGG_w
Cuts at: 0 196 545 1079 1980
Size: 196 349 534 901
Fragments arranged by size:
901 534 349 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 583 613 1980
Size: 583 30 1367
Fragments arranged by size:
1367 583 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 1980
Size: 334 1646
BseYI C'CCAG_C
Cuts at: 0 377 1022 1684 1980
Size: 377 645 662 296
Fragments arranged by size:
662 645 377 296
BsiHKAI G_wGCw'C
Cuts at: 0 8 1048 1980
Size: 8 1040 932
Fragments arranged by size:
1040 932 8
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1332 1980
Size: 1332 648
Bsp1286I G_dGCh'C
Cuts at: 0 8 642 1048 1383 1685 1780 1980
Size: 8 634 406 335 302 95 200
Fragments arranged by size:
634 406 335 302 200 95 8
BspEI T'CCGG_A
Cuts at: 0 545 1980
Size: 545 1435
BspHI T'CATG_A
Cuts at: 0 833 1980
Size: 833 1147
BsrBI CCG'CTC
Cuts at: 0 1036 1980
Size: 1036 944
BsrDI GCAATG_nn'
Cuts at: 0 746 965 1980
Size: 746 219 1015
Fragments arranged by size:
1015 746 219
BsrGI T'GTAC_A
Cuts at: 0 278 1487 1980
Size: 278 1209 493
Fragments arranged by size:
1209 493 278
BstAPI GCAn_nnn'nTGC
Cuts at: 0 751 855 1980
Size: 751 104 1125
Fragments arranged by size:
1125 751 104
BstEII G'GTnAC_C
Cuts at: 0 1168 1980
Size: 1168 812
BstYI r'GATC_y
Cuts at: 0 1839 1845 1980
Size: 1839 6 135
Fragments arranged by size:
1839 135 6
Bsu36I CC'TnA_GG
Cuts at: 0 254 1980
Size: 254 1726
BtgI C'CryG_G
Cuts at: 0 512 1980
Size: 512 1468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 461 1805 1980
Size: 461 1344 175
Fragments arranged by size:
1344 461 175
BtsI GCAGTG_nn'
Cuts at: 0 983 1574 1973 1980
Size: 983 591 399 7
Fragments arranged by size:
983 591 399 7
ClaI AT'CG_AT
Cuts at: 0 389 1980
Size: 389 1591
DraIII CAC_nnn'GTG
Cuts at: 0 276 1927 1980
Size: 276 1651 53
Fragments arranged by size:
1651 276 53
DrdI GACnn_nn'nnGTC
Cuts at: 0 466 1980
Size: 466 1514
EaeI y'GGCC_r
Cuts at: 0 509 1980
Size: 509 1471
EarI CTCTTCn'nnn_
Cuts at: 0 1660 1980
Size: 1660 320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 652 931 1108 1172 1980
Size: 618 34 279 177 64 808
Fragments arranged by size:
808 618 279 177 64 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1784 1980
Size: 1784 196
EcoO109I rG'GnC_Cy
Cuts at: 0 1608 1980
Size: 1608 372
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 551 583 828 860 1331 1363
Size: 37 32 482 32 245 32 471 32
Cuts at: 1363 1855 1887 1980
Size: 492 32 93
Fragments arranged by size:
492 482 471 245 93 37 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 717 1980
Size: 717 1263
MslI CAynn'nnrTG
Cuts at: 0 109 412 526 1151 1660 1980
Size: 109 303 114 625 509 320
Fragments arranged by size:
625 509 320 303 114 109
NspI r_CATG'y
Cuts at: 0 1921 1980
Size: 1921 59
PflMI CCAn_nnn'nTGG
Cuts at: 0 1909 1980
Size: 1909 71
PfoI T'CCnGG_A
Cuts at: 0 690 1771 1980
Size: 690 1081 209
Fragments arranged by size:
1081 690 209
PsiI TTA'TAA
Cuts at: 0 584 1980
Size: 584 1396
PstI C_TGCA'G
Cuts at: 0 759 851 1980
Size: 759 92 1129
Fragments arranged by size:
1129 759 92
SfcI C'TryA_G
Cuts at: 0 462 755 847 1980
Size: 462 293 92 1133
Fragments arranged by size:
1133 462 293 92
SmlI C'TyrA_G
Cuts at: 0 1348 1980
Size: 1348 632
SphI G_CATG'C
Cuts at: 0 1921 1980
Size: 1921 59
StyI C'CwwG_G
Cuts at: 0 20 1980
Size: 20 1960
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 369 1886 1980
Size: 369 1517 94
Fragments arranged by size:
1517 369 94
TatI w'GTAC_w
Cuts at: 0 278 1487 1980
Size: 278 1209 493
Fragments arranged by size:
1209 493 278
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1279 1311 1980
Size: 1279 32 669
Fragments arranged by size:
1279 669 32
XbaI T'CTAG_A
Cuts at: 0 1161 1980
Size: 1161 819
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1744 1980
Size: 1744 236
XmnI GAAnn'nnTTC
Cuts at: 0 1093 1235 1631 1980
Size: 1093 142 396 349
Fragments arranged by size:
1093 396 349 142
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlwNI ApoI AseI
BanI BanII BbsI BclI BglII BlpI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI
BsrGI BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I
Hin4I HincII MslI NspI PflMI PfoI PsiI PstI
SfcI SmlI SphI StyI TaqII TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AlfI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BfrBI BglI BmrI
BmtI BplI BsaAI BsaHI BsgI BsiEI BsiWI BsmI
BspMI BsrFI BssHII BssSI BstBI BstXI BstZ17I CspCI
DraI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XhoI
XmaI ZraI