Restriction map for
pFN21AE5733
(Linear) (Six-base) MAPSORT of: KIEE5733.seq Check: 6291 from: 1 to: 1926
KIEE5733.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1568 1926
Size: 1568 358
AatII G_ACGT'C
Cuts at: 0 1379 1926
Size: 1379 547
AccI GT'mk_AC
Cuts at: 0 1700 1926
Size: 1700 226
AflIII A'CryG_T
Cuts at: 0 221 1926
Size: 221 1705
AgeI A'CCGG_T
Cuts at: 0 143 1926
Size: 143 1783
AlwNI CAG_nnn'CTG
Cuts at: 0 652 676 897 1518 1926
Size: 652 24 221 621 408
Fragments arranged by size:
652 621 408 221 24
ApaI G_GGCC'C
Cuts at: 0 794 835 1926
Size: 794 41 1091
Fragments arranged by size:
1091 794 41
AvaI C'yCGr_G
Cuts at: 0 304 775 1417 1496 1777 1926
Size: 304 471 642 79 281 149
Fragments arranged by size:
642 471 304 281 149 79
BamHI G'GATC_C
Cuts at: 0 1748 1926
Size: 1748 178
BanI G'GyrC_C
Cuts at: 0 33 385 1436 1916 1926
Size: 33 352 1051 480 10
Fragments arranged by size:
1051 480 352 33 10
BanII G_rGCy'C
Cuts at: 0 100 335 659 749 794 835 1417 1427
Size: 100 235 324 90 45 41 582 10
Cuts at: 1427 1618 1926
Size: 191 308
Fragments arranged by size:
582 324 308 235 191 100 90 45
41 10
BbeI G_GCGC'C
Cuts at: 0 1920 1926
Size: 1920 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 865 908 971 980 1926
Size: 865 43 63 9 946
Fragments arranged by size:
946 865 63 43 9
BbvCI CC'TCA_GC
Cuts at: 0 599 1832 1926
Size: 599 1233 94
Fragments arranged by size:
1233 599 94
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 571 605 1926
Size: 571 34 1321
Fragments arranged by size:
1321 571 34
BglI GCCn_nnn'nGGC
Cuts at: 0 713 1926
Size: 713 1213
BlpI GC'TnA_GC
Cuts at: 0 1861 1926
Size: 1861 65
Bme1580I G_kGCm'C
Cuts at: 0 390 794 835 1441 1926
Size: 390 404 41 606 485
Fragments arranged by size:
606 485 404 390 41
BmgBI CAC'GTC
Cuts at: 0 222 1767 1926
Size: 222 1545 159
Fragments arranged by size:
1545 222 159
BmtI G_CTAG'C
Cuts at: 0 473 1926
Size: 473 1453
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 192 1926
Size: 192 1734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 698 763 919 979 1602 1672 1711
Size: 398 300 65 156 60 623 70 39
Cuts at: 1711 1926
Size: 215
Fragments arranged by size:
623 398 300 215 156 70 65 60
39
Bpu10I CC'TnA_GC
Cuts at: 0 334 599 653 1612 1832 1926
Size: 334 265 54 959 220 94
Fragments arranged by size:
959 334 265 220 94 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 152 1636 1926
Size: 152 1484 290
Fragments arranged by size:
1484 290 152
BsaAI yAC'GTr
Cuts at: 0 1629 1926
Size: 1629 297
BsaHI Gr'CG_yC
Cuts at: 0 1376 1697 1917 1926
Size: 1376 321 220 9
Fragments arranged by size:
1376 321 220 9
BsaWI w'CCGG_w
Cuts at: 0 143 1286 1745 1751 1926
Size: 143 1143 459 6 175
Fragments arranged by size:
1143 459 175 143 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 243 273 892 922 1926
Size: 243 30 619 30 1004
Fragments arranged by size:
1004 619 243 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 168 189 264 994 1435 1849 1926
Size: 168 21 75 730 441 414 77
Fragments arranged by size:
730 441 414 168 77 75 21
BseYI C'CCAG_C
Cuts at: 0 372 542 645 828 1926
Size: 372 170 103 183 1098
Fragments arranged by size:
1098 372 183 170 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1926
Size: 757 1169
BsiEI CG_ry'CG
Cuts at: 0 64 1482 1746 1926
Size: 64 1418 264 180
Fragments arranged by size:
1418 264 180 64
BsiHKAI G_wGCw'C
Cuts at: 0 1618 1727 1926
Size: 1618 109 199
Fragments arranged by size:
1618 199 109
BsmI GAATG_Cn'
Cuts at: 0 688 1037 1926
Size: 688 349 889
Fragments arranged by size:
889 688 349
BsmBI CGTCTCn'nnnn_
Cuts at: 0 212 1926
Size: 212 1714
Bsp1286I G_dGCh'C
Cuts at: 0 100 335 390 659 749 794 835 1417
Size: 100 235 55 269 90 45 41 582
Cuts at: 1417 1427 1441 1618 1727 1926
Size: 10 14 177 109 199
Fragments arranged by size:
582 269 235 199 177 109 100 90
55 45 41 14 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1568 1697 1926
Size: 1568 129 229
Fragments arranged by size:
1568 229 129
BsrBI CCG'CTC
Cuts at: 0 1484 1908 1926
Size: 1484 424 18
Fragments arranged by size:
1484 424 18
BsrFI r'CCGG_y
Cuts at: 0 143 1913 1926
Size: 143 1770 13
Fragments arranged by size:
1770 143 13
BstBI TT'CG_AA
Cuts at: 0 193 1926
Size: 193 1733
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1595 1926
Size: 1595 331
BstYI r'GATC_y
Cuts at: 0 487 1748 1926
Size: 487 1261 178
Fragments arranged by size:
1261 487 178
BtgI C'CryG_G
Cuts at: 0 164 213 749 929 1427 1926
Size: 164 49 536 180 498 499
Fragments arranged by size:
536 499 498 180 164 49
BtsI GCAGTG_nn'
Cuts at: 0 635 1082 1926
Size: 635 447 844
Fragments arranged by size:
844 635 447
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 406 441 1926
Size: 406 35 1485
Fragments arranged by size:
1485 406 35
DraI TTT'AAA
Cuts at: 0 1181 1926
Size: 1181 745
DrdI GACnn_nn'nnGTC
Cuts at: 0 1282 1926
Size: 1282 644
EaeI y'GGCC_r
Cuts at: 0 1069 1926
Size: 1069 857
EarI CTCTTCn'nnn_
Cuts at: 0 212 917 1898 1926
Size: 212 705 981 28
Fragments arranged by size:
981 705 212 28
EcoICRI GAG'CTC
Cuts at: 0 1616 1926
Size: 1616 310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 698 763 919 979 1602 1672 1711
Size: 398 300 65 156 60 623 70 39
Cuts at: 1711 1926
Size: 215
Fragments arranged by size:
623 398 300 215 156 70 65 60
39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 499 1926
Size: 499 1427
EcoO109I rG'GnC_Cy
Cuts at: 0 300 790 1852 1926
Size: 300 490 1062 74
Fragments arranged by size:
1062 490 300 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 489 1926
Size: 489 1437
HaeII r_GCGC'y
Cuts at: 0 1920 1926
Size: 1920 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 93 125 181 213 388 420 898 930
Size: 93 32 56 32 175 32 478 32
Cuts at: 930 1926
Size: 996
Fragments arranged by size:
996 478 175 93 56 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1390 1926
Size: 1390 536
KasI G'GCGC_C
Cuts at: 0 1916 1926
Size: 1916 10
MscI TGG'CCA
Cuts at: 0 1071 1926
Size: 1071 855
MspA1I CmG'CkG
Cuts at: 0 215 376 542 741 1668 1926
Size: 215 161 166 199 927 258
Fragments arranged by size:
927 258 215 199 166 161
NarI GG'CG_CC
Cuts at: 0 1917 1926
Size: 1917 9
NcoI C'CATG_G
Cuts at: 0 164 749 929 1926
Size: 164 585 180 997
Fragments arranged by size:
997 585 180 164
NheI G'CTAG_C
Cuts at: 0 469 1926
Size: 469 1457
NspI r_CATG'y
Cuts at: 0 175 1926
Size: 175 1751
PasI CC'CwG_GG
Cuts at: 0 1443 1926
Size: 1443 483
PflMI CCAn_nnn'nTGG
Cuts at: 0 897 1518 1710 1926
Size: 897 621 192 216
Fragments arranged by size:
897 621 216 192
PfoI T'CCnGG_A
Cuts at: 0 956 1319 1926
Size: 956 363 607
Fragments arranged by size:
956 607 363
PpuMI rG'GwC_Cy
Cuts at: 0 300 1926
Size: 300 1626
PshAI GACnn'nnGTC
Cuts at: 0 614 1926
Size: 614 1312
PspOMI G'GGCC_C
Cuts at: 0 790 831 1926
Size: 790 41 1095
Fragments arranged by size:
1095 790 41
PstI C_TGCA'G
Cuts at: 0 126 1604 1694 1926
Size: 126 1478 90 232
Fragments arranged by size:
1478 232 126 90
PvuII CAG'CTG
Cuts at: 0 376 542 741 1668 1926
Size: 376 166 199 927 258
Fragments arranged by size:
927 376 258 199 166
SacI G_AGCT'C
Cuts at: 0 1618 1926
Size: 1618 308
SacII CC_GC'GG
Cuts at: 0 216 1926
Size: 216 1710
SapI GCTCTTCn'nnn_
Cuts at: 0 1898 1926
Size: 1898 28
SbfI CC_TGCA'GG
Cuts at: 0 1694 1926
Size: 1694 232
SexAI A'CCwGG_T
Cuts at: 0 1673 1926
Size: 1673 253
SfcI C'TryA_G
Cuts at: 0 54 122 1278 1600 1690 1926
Size: 54 68 1156 322 90 236
Fragments arranged by size:
1156 322 236 90 68 54
SfoI GGC'GCC
Cuts at: 0 1918 1926
Size: 1918 8
SmaI CCC'GGG
Cuts at: 0 777 1498 1926
Size: 777 721 428
Fragments arranged by size:
777 721 428
SspI AAT'ATT
Cuts at: 0 1327 1883 1926
Size: 1327 556 43
Fragments arranged by size:
1327 556 43
StyI C'CwwG_G
Cuts at: 0 164 749 929 1541 1718 1926
Size: 164 585 180 612 177 208
Fragments arranged by size:
612 585 208 180 177 164
TatI w'GTAC_w
Cuts at: 0 1526 1841 1926
Size: 1526 315 85
Fragments arranged by size:
1526 315 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 243 275 292 324 892 924 977 1009
Size: 243 32 17 32 568 32 53 32
Cuts at: 1009 1926
Size: 917
Fragments arranged by size:
917 568 243 53 32 32 32 32
17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 397 1515 1926
Size: 397 1118 411
Fragments arranged by size:
1118 411 397
XmaI C'CCGG_G
Cuts at: 0 775 1496 1926
Size: 775 721 430
Fragments arranged by size:
775 721 430
XmnI GAAnn'nnTTC
Cuts at: 0 1156 1926
Size: 1156 770
ZraI GAC'GTC
Cuts at: 0 1377 1926
Size: 1377 549
Enzymes that do cut:
AarI AatII AccI AflIII AgeI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI
BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BstBI
BstXI BstYI BtgI BtsI CspCI DraI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HindIII KasI MscI MspA1I NarI NcoI NheI NspI
PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII
SacI SacII SapI SbfI SexAI SfcI SfoI SmaI
SspI StyI TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BciVI BclI BfrBI BglII BmrI BpuEI BsaBI BsiWI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstEII
BstZ17I Bsu36I BtgZI ClaI DraIII EagI EciI EcoRI
EcoRV FseI FspI FspAI HincII HpaI KpnI MfeI
MluI MmeI MslI NaeI NdeI NgoMIV NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SalI SanDI ScaI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI StuI SwaI
TaqII Tth111I XbaI XhoI