Restriction map for
pFN21AE5732
(Linear) (Six-base) MAPSORT of: KIEE5732.seq Check: 4099 from: 1 to: 1908
KIEE5732.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 559 1908
Size: 559 1349
AccI GT'mk_AC
Cuts at: 0 80 1109 1908
Size: 80 1029 799
Fragments arranged by size:
1029 799 80
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1476 1908
Size: 862 614 432
Fragments arranged by size:
862 614 432
AflIII A'CryG_T
Cuts at: 0 938 1040 1908
Size: 938 102 868
Fragments arranged by size:
938 868 102
AlwNI CAG_nnn'CTG
Cuts at: 0 1053 1090 1499 1908
Size: 1053 37 409 409
Fragments arranged by size:
1053 409 409 37
ApaI G_GGCC'C
Cuts at: 0 314 534 1908
Size: 314 220 1374
Fragments arranged by size:
1374 314 220
ApaLI G'TGCA_C
Cuts at: 0 1816 1908
Size: 1816 92
AvrII C'CTAG_G
Cuts at: 0 306 1908
Size: 306 1602
BanI G'GyrC_C
Cuts at: 0 217 349 1908
Size: 217 132 1559
Fragments arranged by size:
1559 217 132
BanII G_rGCy'C
Cuts at: 0 21 284 314 534 917 1286 1690 1908
Size: 21 263 30 220 383 369 404 218
Fragments arranged by size:
404 383 369 263 220 218 30 21
BbeI G_GCGC'C
Cuts at: 0 221 353 1908
Size: 221 132 1555
Fragments arranged by size:
1555 221 132
BbsI GAAGACnn'nnnn_
Cuts at: 0 704 842 1908
Size: 704 138 1066
Fragments arranged by size:
1066 704 138
BciVI GTATCCnnnnn_n'
Cuts at: 0 703 1908
Size: 703 1205
BclI T'GATC_A
Cuts at: 0 404 1908
Size: 404 1504
BglI GCCn_nnn'nGGC
Cuts at: 0 1783 1908
Size: 1783 125
BglII A'GATC_T
Cuts at: 0 881 1908
Size: 881 1027
BlpI GC'TnA_GC
Cuts at: 0 1604 1908
Size: 1604 304
Bme1580I G_kGCm'C
Cuts at: 0 233 314 534 1820 1908
Size: 233 81 220 1286 88
Fragments arranged by size:
1286 233 220 88 81
BmrI ACTGGGnnnn_n'
Cuts at: 0 56 825 1743 1908
Size: 56 769 918 165
Fragments arranged by size:
918 769 165 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 40 805 1908
Size: 40 765 1103
Fragments arranged by size:
1103 765 40
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 298 520 600 1231 1768 1908
Size: 285 13 222 80 631 537 140
Fragments arranged by size:
631 537 285 222 140 80 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 1908
Size: 682 1226
BsaI GGTCTCn'nnnn_
Cuts at: 0 1207 1908
Size: 1207 701
BsaAI yAC'GTr
Cuts at: 0 417 1406 1908
Size: 417 989 502
Fragments arranged by size:
989 502 417
BsaHI Gr'CG_yC
Cuts at: 0 218 350 1908
Size: 218 132 1558
Fragments arranged by size:
1558 218 132
BsaWI w'CCGG_w
Cuts at: 0 1757 1908
Size: 1757 151
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 496 526 1882 1908
Size: 496 30 1356 26
Fragments arranged by size:
1356 496 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 227 586 907 1765 1903 1906 1908
Size: 227 359 321 858 138 3 2
Fragments arranged by size:
858 359 321 227 138 3 2
BseYI C'CCAG_C
Cuts at: 0 565 1032 1053 1467 1518 1611 1650 1786
Size: 565 467 21 414 51 93 39 136
Cuts at: 1786 1908
Size: 122
Fragments arranged by size:
565 467 414 136 122 93 51 39
21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 537 1908
Size: 86 451 1371
Fragments arranged by size:
1371 451 86
BsiEI CG_ry'CG
Cuts at: 0 1723 1908
Size: 1723 185
BsiHKAI G_wGCw'C
Cuts at: 0 21 284 422 467 506 1820 1908
Size: 21 263 138 45 39 1314 88
Fragments arranged by size:
1314 263 138 88 45 39 21
BsmI GAATG_Cn'
Cuts at: 0 1433 1908
Size: 1433 475
Bsp1286I G_dGCh'C
Cuts at: 0 21 233 284 314 422 467 506 534
Size: 21 212 51 30 108 45 39 28
Cuts at: 534 917 1286 1690 1820 1908
Size: 383 369 404 130 88
Fragments arranged by size:
404 383 369 212 130 108 88 51
45 39 30 28 21
BspHI T'CATG_A
Cuts at: 0 1643 1908
Size: 1643 265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 56 559 1262 1908
Size: 56 503 703 646
Fragments arranged by size:
703 646 503 56
BsrBI CCG'CTC
Cuts at: 0 1701 1908
Size: 1701 207
BsrFI r'CCGG_y
Cuts at: 0 352 1718 1722 1908
Size: 352 1366 4 186
Fragments arranged by size:
1366 352 186 4
BssSI C'ACGA_G
Cuts at: 0 226 1908
Size: 226 1682
BstXI CCAn_nnnn'nTGG
Cuts at: 0 800 1005 1530 1869 1908
Size: 800 205 525 339 39
Fragments arranged by size:
800 525 339 205 39
BstYI r'GATC_y
Cuts at: 0 635 881 1908
Size: 635 246 1027
Fragments arranged by size:
1027 635 246
BtgI C'CryG_G
Cuts at: 0 777 1727 1908
Size: 777 950 181
Fragments arranged by size:
950 777 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 89 393 1908
Size: 89 304 1515
Fragments arranged by size:
1515 304 89
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1392 1427 1908
Size: 1392 35 481
Fragments arranged by size:
1392 481 35
DraIII CAC_nnn'GTG
Cuts at: 0 1406 1908
Size: 1406 502
EaeI y'GGCC_r
Cuts at: 0 248 386 737 1295 1720 1724 1775 1859
Size: 248 138 351 558 425 4 51 84
Cuts at: 1859 1908
Size: 49
Fragments arranged by size:
558 425 351 248 138 84 51 49
4
EagI C'GGCC_G
Cuts at: 0 1720 1908
Size: 1720 188
EarI CTCTTCn'nnn_
Cuts at: 0 274 346 427 436 881 1165 1676 1908
Size: 274 72 81 9 445 284 511 232
Fragments arranged by size:
511 445 284 274 232 81 72 9
EcoICRI GAG'CTC
Cuts at: 0 19 282 1908
Size: 19 263 1626
Fragments arranged by size:
1626 263 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 298 520 600 862 1231 1476 1768
Size: 285 13 222 80 262 369 245 292
Cuts at: 1768 1908
Size: 140
Fragments arranged by size:
369 292 285 262 245 222 140 80
13
EcoO109I rG'GnC_Cy
Cuts at: 0 310 311 530 531 797 1087 1496 1908
Size: 310 1 219 1 266 290 409 412
Fragments arranged by size:
412 409 310 290 266 219 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 150 892 1908
Size: 150 742 1016
Fragments arranged by size:
1016 742 150
FseI GG_CCGG'CC
Cuts at: 0 1726 1908
Size: 1726 182
FspI TGC'GCA
Cuts at: 0 1825 1908
Size: 1825 83
HaeII r_GCGC'y
Cuts at: 0 186 221 353 1373 1908
Size: 186 35 132 1020 535
Fragments arranged by size:
1020 535 186 132 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1882 1908
Size: 1882 26
HincII GTy'rAC
Cuts at: 0 81 1908
Size: 81 1827
KasI G'GCGC_C
Cuts at: 0 217 349 1908
Size: 217 132 1559
Fragments arranged by size:
1559 217 132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1731 1908
Size: 1731 177
MscI TGG'CCA
Cuts at: 0 250 388 1297 1777 1908
Size: 250 138 909 480 131
Fragments arranged by size:
909 480 250 138 131
MslI CAynn'nnrTG
Cuts at: 0 1126 1291 1459 1621 1663 1771 1867 1908
Size: 1126 165 168 162 42 108 96 41
Fragments arranged by size:
1126 168 165 162 108 96 42 41
MspA1I CmG'CkG
Cuts at: 0 641 779 1228 1654 1908
Size: 641 138 449 426 254
Fragments arranged by size:
641 449 426 254 138
NaeI GCC'GGC
Cuts at: 0 354 1720 1724 1908
Size: 354 1366 4 184
Fragments arranged by size:
1366 354 184 4
NarI GG'CG_CC
Cuts at: 0 218 350 1908
Size: 218 132 1558
Fragments arranged by size:
1558 218 132
NcoI C'CATG_G
Cuts at: 0 1727 1908
Size: 1727 181
NgoMIV G'CCGG_C
Cuts at: 0 352 1718 1722 1908
Size: 352 1366 4 186
Fragments arranged by size:
1366 352 186 4
NspI r_CATG'y
Cuts at: 0 1044 1341 1662 1770 1824 1908
Size: 1044 297 321 108 54 84
Fragments arranged by size:
1044 321 297 108 84 54
PasI CC'CwG_GG
Cuts at: 0 288 458 628 793 894 1598 1908
Size: 288 170 170 165 101 704 310
Fragments arranged by size:
704 310 288 170 170 165 101
PciI A'CATG_T
Cuts at: 0 1040 1908
Size: 1040 868
PflMI CCAn_nnn'nTGG
Cuts at: 0 860 1733 1908
Size: 860 873 175
Fragments arranged by size:
873 860 175
PfoI T'CCnGG_A
Cuts at: 0 275 1908
Size: 275 1633
PmlI CAC'GTG
Cuts at: 0 1406 1908
Size: 1406 502
PspOMI G'GGCC_C
Cuts at: 0 310 530 1908
Size: 310 220 1378
Fragments arranged by size:
1378 310 220
PvuII CAG'CTG
Cuts at: 0 1228 1654 1908
Size: 1228 426 254
Fragments arranged by size:
1228 426 254
SacI G_AGCT'C
Cuts at: 0 21 284 1908
Size: 21 263 1624
Fragments arranged by size:
1624 263 21
SacII CC_GC'GG
Cuts at: 0 780 1908
Size: 780 1128
SalI G'TCGA_C
Cuts at: 0 79 1908
Size: 79 1829
SapI GCTCTTCn'nnn_
Cuts at: 0 427 881 1908
Size: 427 454 1027
Fragments arranged by size:
1027 454 427
ScaI AGT'ACT
Cuts at: 0 577 1908
Size: 577 1331
SfcI C'TryA_G
Cuts at: 0 957 1365 1908
Size: 957 408 543
Fragments arranged by size:
957 543 408
SfoI GGC'GCC
Cuts at: 0 219 351 1908
Size: 219 132 1557
Fragments arranged by size:
1557 219 132
SgrAI Cr'CCGG_yG
Cuts at: 0 352 1908
Size: 352 1556
SmlI C'TyrA_G
Cuts at: 0 697 1908
Size: 697 1211
SphI G_CATG'C
Cuts at: 0 1662 1908
Size: 1662 246
StuI AGG'CCT
Cuts at: 0 167 1908
Size: 167 1741
StyI C'CwwG_G
Cuts at: 0 306 515 603 854 1727 1908
Size: 306 209 88 251 873 181
Fragments arranged by size:
873 306 251 209 181 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 886 1908
Size: 886 1022
TatI w'GTAC_w
Cuts at: 0 575 1340 1908
Size: 575 765 568
Fragments arranged by size:
765 575 568
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 496 528 1908
Size: 496 32 1380
Fragments arranged by size:
1380 496 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1305 1908
Size: 1305 603
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApaI ApaLI AvrII
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstXI
BstYI BtgI BtgZI CspCI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoO109I FalI FseI FspI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI
PspOMI PvuII SacI SacII SalI SapI ScaI SfcI
SfoI SgrAI SmlI SphI StuI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvaI BaeI
BamHI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaBI
BsiWI BsmBI BspEI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI DraI DrdI EciI
EcoNI EcoRI EcoRV FspAI HindIII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SanDI SbfI SexAI SfiI SgfI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI