Restriction map for
pFN21AE5731
(Linear) (Six-base) MAPSORT of: KIEE5731.seq Check: 8527 from: 1 to: 1779
KIEE5731.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AatII G_ACGT'C
Cuts at: 0 1766 1779
Size: 1766 13
AccI GT'mk_AC
Cuts at: 0 548 1522 1562 1766 1779
Size: 548 974 40 204 13
Fragments arranged by size:
974 548 204 40 13
AclI AA'CG_TT
Cuts at: 0 1580 1779
Size: 1580 199
AflIII A'CryG_T
Cuts at: 0 242 1034 1779
Size: 242 792 745
Fragments arranged by size:
792 745 242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 658 1779
Size: 658 1121
AlwNI CAG_nnn'CTG
Cuts at: 0 1504 1779
Size: 1504 275
ApaI G_GGCC'C
Cuts at: 0 193 1100 1779
Size: 193 907 679
Fragments arranged by size:
907 679 193
ApaLI G'TGCA_C
Cuts at: 0 856 1779
Size: 856 923
ApoI r'AATT_y
Cuts at: 0 1281 1589 1779
Size: 1281 308 190
Fragments arranged by size:
1281 308 190
AscI GG'CGCG_CC
Cuts at: 0 83 1779
Size: 83 1696
AvaI C'yCGr_G
Cuts at: 0 67 411 1192 1779
Size: 67 344 781 587
Fragments arranged by size:
781 587 344 67
AvrII C'CTAG_G
Cuts at: 0 1177 1779
Size: 1177 602
BamHI G'GATC_C
Cuts at: 0 470 1779
Size: 470 1309
BanI G'GyrC_C
Cuts at: 0 63 261 1275 1779
Size: 63 198 1014 504
Fragments arranged by size:
1014 504 198 63
BanII G_rGCy'C
Cuts at: 0 193 392 925 1100 1170 1353 1779
Size: 193 199 533 175 70 183 426
Fragments arranged by size:
533 426 199 193 183 175 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 210 1779
Size: 210 1569
BbvCI CC'TCA_GC
Cuts at: 0 1010 1779
Size: 1010 769
BciVI GTATCCnnnnn_n'
Cuts at: 0 1696 1779
Size: 1696 83
BclI T'GATC_A
Cuts at: 0 1427 1779
Size: 1427 352
BglI GCCn_nnn'nGGC
Cuts at: 0 425 559 1348 1779
Size: 425 134 789 431
Fragments arranged by size:
789 431 425 134
Bme1580I G_kGCm'C
Cuts at: 0 68 193 266 860 1100 1779
Size: 68 125 73 594 240 679
Fragments arranged by size:
679 594 240 125 73 68
BmgBI CAC'GTC
Cuts at: 0 243 540 708 720 1779
Size: 243 297 168 12 1059
Fragments arranged by size:
1059 297 243 168 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 696 1300 1779
Size: 696 604 479
Fragments arranged by size:
696 604 479
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 987 1779
Size: 987 792
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 937 952 1361 1541 1622 1779
Size: 523 414 15 409 180 81 157
Fragments arranged by size:
523 414 409 180 157 81 15
Bpu10I CC'TnA_GC
Cuts at: 0 1010 1779
Size: 1010 769
BsaI GGTCTCn'nnnn_
Cuts at: 0 721 760 1779
Size: 721 39 1019
Fragments arranged by size:
1019 721 39
BsaAI yAC'GTr
Cuts at: 0 747 1035 1623 1779
Size: 747 288 588 156
Fragments arranged by size:
747 588 288 156
BsaHI Gr'CG_yC
Cuts at: 0 1525 1763 1779
Size: 1525 238 16
Fragments arranged by size:
1525 238 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 910 940 1024 1054 1557 1587 1779
Size: 910 30 84 30 503 30 192
Fragments arranged by size:
910 503 192 84 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 520 1779
Size: 520 1259
BseYI C'CCAG_C
Cuts at: 0 182 1027 1455 1779
Size: 182 845 428 324
Fragments arranged by size:
845 428 324 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1172 1465 1779
Size: 1172 293 314
Fragments arranged by size:
1172 314 293
BsiEI CG_ry'CG
Cuts at: 0 44 563 1487 1655 1688 1779
Size: 44 519 924 168 33 91
Fragments arranged by size:
924 519 168 91 44 33
BsiHKAI G_wGCw'C
Cuts at: 0 392 860 1424 1779
Size: 392 468 564 355
Fragments arranged by size:
564 468 392 355
Bsp1286I G_dGCh'C
Cuts at: 0 68 193 266 392 860 925 1100 1170
Size: 68 125 73 126 468 65 175 70
Cuts at: 1170 1353 1424 1779
Size: 183 71 355
Fragments arranged by size:
468 355 183 175 126 125 73 71
70 68 65
BspHI T'CATG_A
Cuts at: 0 305 758 1233 1779
Size: 305 453 475 546
Fragments arranged by size:
546 475 453 305
BsrDI GCAATG_nn'
Cuts at: 0 1436 1779
Size: 1436 343
BssHII G'CGCG_C
Cuts at: 0 83 375 1779
Size: 83 292 1404
Fragments arranged by size:
1404 292 83
BssSI C'ACGA_G
Cuts at: 0 364 924 1779
Size: 364 560 855
Fragments arranged by size:
855 560 364
BstEII G'GTnAC_C
Cuts at: 0 519 1779
Size: 519 1260
BstXI CCAn_nnnn'nTGG
Cuts at: 0 954 1779
Size: 954 825
BstYI r'GATC_y
Cuts at: 0 230 404 470 737 836 1604 1779
Size: 230 174 66 267 99 768 175
Fragments arranged by size:
768 267 230 175 174 99 66
BstZ17I GTA'TAC
Cuts at: 0 1563 1779
Size: 1563 216
Bsu36I CC'TnA_GG
Cuts at: 0 1721 1779
Size: 1721 58
BtgI C'CryG_G
Cuts at: 0 278 330 579 623 680 953 1212 1481
Size: 278 52 249 44 57 273 259 269
Cuts at: 1481 1682 1779
Size: 201 97
Fragments arranged by size:
278 273 269 259 249 201 97 57
52 44
BtsI GCAGTG_nn'
Cuts at: 0 39 100 386 1779
Size: 39 61 286 1393
Fragments arranged by size:
1393 286 61 39
DraIII CAC_nnn'GTG
Cuts at: 0 1416 1779
Size: 1416 363
DrdI GACnn_nn'nnGTC
Cuts at: 0 544 1779
Size: 544 1235
EaeI y'GGCC_r
Cuts at: 0 41 56 560 648 798 944 1209 1484
Size: 41 15 504 88 150 146 265 275
Cuts at: 1484 1685 1779
Size: 201 94
Fragments arranged by size:
504 275 265 201 150 146 94 88
41 15
EagI C'GGCC_G
Cuts at: 0 41 560 1484 1685 1779
Size: 41 519 924 201 94
Fragments arranged by size:
924 519 201 94 41
EarI CTCTTCn'nnn_
Cuts at: 0 463 784 1779
Size: 463 321 995
Fragments arranged by size:
995 463 321
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 397 1779
Size: 397 1382
EcoICRI GAG'CTC
Cuts at: 0 390 1779
Size: 390 1389
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 937 952 1361 1541 1622 1779
Size: 523 414 15 409 180 81 157
Fragments arranged by size:
523 414 409 180 157 81 15
EcoO109I rG'GnC_Cy
Cuts at: 0 50 189 1174 1749 1779
Size: 50 139 985 575 30
Fragments arranged by size:
985 575 139 50 30
EcoRI G'AATT_C
Cuts at: 0 1589 1779
Size: 1589 190
EcoRV GAT'ATC
Cuts at: 0 1231 1779
Size: 1231 548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 810 1779
Size: 810 969
FspI TGC'GCA
Cuts at: 0 751 1779
Size: 751 1028
HaeII r_GCGC'y
Cuts at: 0 112 1779
Size: 112 1667
HincII GTy'rAC
Cuts at: 0 1302 1523 1779
Size: 1302 221 256
Fragments arranged by size:
1302 256 221
MscI TGG'CCA
Cuts at: 0 946 1779
Size: 946 833
MslI CAynn'nnrTG
Cuts at: 0 1441 1779
Size: 1441 338
MspA1I CmG'CkG
Cuts at: 0 332 581 660 1156 1483 1504 1684 1779
Size: 332 249 79 496 327 21 180 95
Fragments arranged by size:
496 332 327 249 180 95 79 21
NcoI C'CATG_G
Cuts at: 0 623 1779
Size: 623 1156
NotI GC'GGCC_GC
Cuts at: 0 1484 1685 1779
Size: 1484 201 94
Fragments arranged by size:
1484 201 94
NspI r_CATG'y
Cuts at: 0 889 1779
Size: 889 890
PasI CC'CwG_GG
Cuts at: 0 1171 1779
Size: 1171 608
PflMI CCAn_nnn'nTGG
Cuts at: 0 1416 1779
Size: 1416 363
PfoI T'CCnGG_A
Cuts at: 0 500 1779
Size: 500 1279
PpuMI rG'GwC_Cy
Cuts at: 0 50 1749 1779
Size: 50 1699 30
Fragments arranged by size:
1699 50 30
PspOMI G'GGCC_C
Cuts at: 0 189 1096 1779
Size: 189 907 683
Fragments arranged by size:
907 683 189
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 319 351 1779
Size: 319 32 1428
Fragments arranged by size:
1428 319 32
PstI C_TGCA'G
Cuts at: 0 439 1779
Size: 439 1340
PvuI CG_AT'CG
Cuts at: 0 1655 1779
Size: 1655 124
PvuII CAG'CTG
Cuts at: 0 660 1156 1779
Size: 660 496 623
Fragments arranged by size:
660 623 496
SacI G_AGCT'C
Cuts at: 0 392 1779
Size: 392 1387
SacII CC_GC'GG
Cuts at: 0 333 582 1484 1685 1779
Size: 333 249 902 201 94
Fragments arranged by size:
902 333 249 201 94
SalI G'TCGA_C
Cuts at: 0 1521 1779
Size: 1521 258
SapI GCTCTTCn'nnn_
Cuts at: 0 784 1779
Size: 784 995
ScaI AGT'ACT
Cuts at: 0 970 1779
Size: 970 809
SexAI A'CCwGG_T
Cuts at: 0 1320 1779
Size: 1320 459
SfcI C'TryA_G
Cuts at: 0 129 435 1643 1663 1779
Size: 129 306 1208 20 116
Fragments arranged by size:
1208 306 129 116 20
SmaI CCC'GGG
Cuts at: 0 413 1779
Size: 413 1366
SphI G_CATG'C
Cuts at: 0 889 1779
Size: 889 890
SrfI GCCC'GGGC
Cuts at: 0 413 1779
Size: 413 1366
StuI AGG'CCT
Cuts at: 0 1726 1779
Size: 1726 53
StyI C'CwwG_G
Cuts at: 0 59 515 623 1177 1779
Size: 59 456 108 554 602
Fragments arranged by size:
602 554 456 108 59
TatI w'GTAC_w
Cuts at: 0 350 968 1037 1779
Size: 350 618 69 742
Fragments arranged by size:
742 618 350 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 910 942 1520 1552 1557 1589 1779
Size: 910 32 578 32 5 32 190
Fragments arranged by size:
910 578 190 32 32 32 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 179 1779
Size: 179 1600
XmaI C'CCGG_G
Cuts at: 0 411 1779
Size: 411 1368
XmnI GAAnn'nnTTC
Cuts at: 0 793 1779
Size: 793 986
ZraI GAC'GTC
Cuts at: 0 1764 1779
Size: 1764 15
Enzymes that do cut:
AatII AccI AclI AflIII AlfI AlwNI ApaI ApaLI
ApoI AscI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BciVI BclI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrDI BssHII BssSI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII HincII MscI MslI
MspA1I NcoI NotI NspI PasI PflMI PfoI PpuMI
PspOMI PsrI PstI PvuI PvuII SacI SacII SalI
SapI ScaI SexAI SfcI SmaI SphI SrfI StuI
StyI TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AcuI AfeI AflII AgeI AhdI AleI
AloI AseI AsiSI BaeI BbeI BcgI BfrBI BglII
BlpI BmtI BpuEI BsaBI BsaWI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstBI BtgZI
ClaI CspCI DraI EcoNI FseI FspAI Hin4I HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI RsrII SanDI SbfI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SspI
SwaI TaqII Tth111I XbaI XhoI