Restriction map for
pFN21AE5730
(Linear) (Six-base) MAPSORT of: KIEE5730.seq Check: 886 from: 1 to: 1890
KIEE5730.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 958 1523 1890
Size: 958 565 367
Fragments arranged by size:
958 565 367
AatII G_ACGT'C
Cuts at: 0 709 1890
Size: 709 1181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1465 1890
Size: 1465 425
AfeI AGC'GCT
Cuts at: 0 793 1890
Size: 793 1097
AflIII A'CryG_T
Cuts at: 0 248 1341 1890
Size: 248 1093 549
Fragments arranged by size:
1093 549 248
AgeI A'CCGG_T
Cuts at: 0 1598 1890
Size: 1598 292
AleI CACnn'nnGTG
Cuts at: 0 514 1890
Size: 514 1376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 553 1573 1834 1890
Size: 553 1020 261 56
Fragments arranged by size:
1020 553 261 56
AlwNI CAG_nnn'CTG
Cuts at: 0 72 1426 1890
Size: 72 1354 464
Fragments arranged by size:
1354 464 72
ApaI G_GGCC'C
Cuts at: 0 159 1890
Size: 159 1731
ApaLI G'TGCA_C
Cuts at: 0 1002 1890
Size: 1002 888
ApoI r'AATT_y
Cuts at: 0 1115 1890
Size: 1115 775
AseI AT'TA_AT
Cuts at: 0 602 1890
Size: 602 1288
AvaI C'yCGr_G
Cuts at: 0 1538 1561 1890
Size: 1538 23 329
Fragments arranged by size:
1538 329 23
BanI G'GyrC_C
Cuts at: 0 325 946 1296 1623 1681 1890
Size: 325 621 350 327 58 209
Fragments arranged by size:
621 350 327 325 209 58
BanII G_rGCy'C
Cuts at: 0 159 473 839 1076 1890
Size: 159 314 366 237 814
Fragments arranged by size:
814 366 314 237 159
BbsI GAAGACnn'nnnn_
Cuts at: 0 217 1296 1890
Size: 217 1079 594
Fragments arranged by size:
1079 594 217
BbvCI CC'TCA_GC
Cuts at: 0 340 1890
Size: 340 1550
BglI GCCn_nnn'nGGC
Cuts at: 0 944 1138 1890
Size: 944 194 752
Fragments arranged by size:
944 752 194
BlpI GC'TnA_GC
Cuts at: 0 838 1890
Size: 838 1052
Bme1580I G_kGCm'C
Cuts at: 0 56 159 858 1006 1301 1464 1628 1890
Size: 56 103 699 148 295 163 164 262
Fragments arranged by size:
699 295 262 164 163 148 103 56
BmgBI CAC'GTC
Cuts at: 0 555 1890
Size: 555 1335
BmrI ACTGGGnnnn_n'
Cuts at: 0 231 1890
Size: 231 1659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 270 844 1246 1352 1890
Size: 217 53 574 402 106 538
Fragments arranged by size:
574 538 402 217 106 53
Bpu10I CC'TnA_GC
Cuts at: 0 340 548 1890
Size: 340 208 1342
Fragments arranged by size:
1342 340 208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1003 1890
Size: 1003 887
BsaI GGTCTCn'nnnn_
Cuts at: 0 169 1699 1796 1890
Size: 169 1530 97 94
Fragments arranged by size:
1530 169 97 94
BsaAI yAC'GTr
Cuts at: 0 80 1890
Size: 80 1810
BsaHI Gr'CG_yC
Cuts at: 0 706 1890
Size: 706 1184
BsaWI w'CCGG_w
Cuts at: 0 1598 1890
Size: 1598 292
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 385 415 848 878 1117 1147 1890
Size: 385 30 433 30 239 30 743
Fragments arranged by size:
743 433 385 239 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 127 468 732 1125 1890
Size: 40 87 341 264 393 765
Fragments arranged by size:
765 393 341 264 87 40
BseYI C'CCAG_C
Cuts at: 0 16 1697 1870 1890
Size: 16 1681 173 20
Fragments arranged by size:
1681 173 20 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1018 1890
Size: 163 855 872
Fragments arranged by size:
872 855 163
BsiHKAI G_wGCw'C
Cuts at: 0 554 839 1006 1076 1890
Size: 554 285 167 70 814
Fragments arranged by size:
814 554 285 167 70
BsmBI CGTCTCn'nnnn_
Cuts at: 0 619 935 1890
Size: 619 316 955
Fragments arranged by size:
955 619 316
Bsp1286I G_dGCh'C
Cuts at: 0 56 159 473 554 839 858 1006 1076
Size: 56 103 314 81 285 19 148 70
Cuts at: 1076 1301 1464 1628 1890
Size: 225 163 164 262
Fragments arranged by size:
314 285 262 225 164 163 148 103
81 70 56 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 92 958 1523 1890
Size: 92 866 565 367
Fragments arranged by size:
866 565 367 92
BsrBI CCG'CTC
Cuts at: 0 201 1890
Size: 201 1689
BsrFI r'CCGG_y
Cuts at: 0 485 1598 1890
Size: 485 1113 292
Fragments arranged by size:
1113 485 292
BssSI C'ACGA_G
Cuts at: 0 748 1863 1890
Size: 748 1115 27
Fragments arranged by size:
1115 748 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 93 1890
Size: 93 1797
BstYI r'GATC_y
Cuts at: 0 627 1890
Size: 627 1263
BtgI C'CryG_G
Cuts at: 0 1634 1890
Size: 1634 256
BtsI GCAGTG_nn'
Cuts at: 0 27 94 1006 1522 1890
Size: 27 67 912 516 368
Fragments arranged by size:
912 516 368 67 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1757 1792 1890
Size: 1757 35 98
Fragments arranged by size:
1757 98 35
DraIII CAC_nnn'GTG
Cuts at: 0 105 1890
Size: 105 1785
DrdI GACnn_nn'nnGTC
Cuts at: 0 1180 1890
Size: 1180 710
EaeI y'GGCC_r
Cuts at: 0 74 495 1361 1484 1499 1890
Size: 74 421 866 123 15 391
Fragments arranged by size:
866 421 391 123 74 15
EarI CTCTTCn'nnn_
Cuts at: 0 121 478 1890
Size: 121 357 1412
Fragments arranged by size:
1412 357 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 598 890 1312 1890
Size: 598 292 422 578
Fragments arranged by size:
598 578 422 292
EcoICRI GAG'CTC
Cuts at: 0 837 1074 1890
Size: 837 237 816
Fragments arranged by size:
837 816 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 270 844 1246 1352 1465 1890
Size: 217 53 574 402 106 113 425
Fragments arranged by size:
574 425 402 217 113 106 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1275 1890
Size: 1275 615
EcoO109I rG'GnC_Cy
Cuts at: 0 155 156 384 1890
Size: 155 1 228 1506
Fragments arranged by size:
1506 228 155 1
HaeII r_GCGC'y
Cuts at: 0 795 1890
Size: 795 1095
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 291 323 385 417 1300 1332 1890
Size: 291 32 62 32 883 32 558
Fragments arranged by size:
883 558 291 62 32 32 32
HincII GTy'rAC
Cuts at: 0 282 1890
Size: 282 1608
HindIII A'AGCT_T
Cuts at: 0 1432 1890
Size: 1432 458
MfeI C'AATT_G
Cuts at: 0 692 1890
Size: 692 1198
MluI A'CGCG_T
Cuts at: 0 1341 1890
Size: 1341 549
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 1890
Size: 692 1198
MscI TGG'CCA
Cuts at: 0 76 1486 1501 1890
Size: 76 1410 15 389
Fragments arranged by size:
1410 389 76 15
MslI CAynn'nnrTG
Cuts at: 0 514 1252 1480 1639 1795 1890
Size: 514 738 228 159 156 95
Fragments arranged by size:
738 514 228 159 156 95
MspA1I CmG'CkG
Cuts at: 0 60 339 432 1266 1739 1890
Size: 60 279 93 834 473 151
Fragments arranged by size:
834 473 279 151 93 60
NspI r_CATG'y
Cuts at: 0 96 252 1890
Size: 96 156 1638
Fragments arranged by size:
1638 156 96
PciI A'CATG_T
Cuts at: 0 248 1890
Size: 248 1642
PflMI CCAn_nnn'nTGG
Cuts at: 0 905 982 1518 1890
Size: 905 77 536 372
Fragments arranged by size:
905 536 372 77
PfoI T'CCnGG_A
Cuts at: 0 194 1331 1890
Size: 194 1137 559
Fragments arranged by size:
1137 559 194
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 1890
Size: 103 32 1755
Fragments arranged by size:
1755 103 32
PspOMI G'GGCC_C
Cuts at: 0 155 1890
Size: 155 1735
PstI C_TGCA'G
Cuts at: 0 65 89 185 926 1813 1890
Size: 65 24 96 741 887 77
Fragments arranged by size:
887 741 96 77 65 24
PvuII CAG'CTG
Cuts at: 0 339 432 1266 1739 1890
Size: 339 93 834 473 151
Fragments arranged by size:
834 473 339 151 93
SacI G_AGCT'C
Cuts at: 0 839 1076 1890
Size: 839 237 814
Fragments arranged by size:
839 814 237
SapI GCTCTTCn'nnn_
Cuts at: 0 121 478 1890
Size: 121 357 1412
Fragments arranged by size:
1412 357 121
ScaI AGT'ACT
Cuts at: 0 235 1890
Size: 235 1655
SfcI C'TryA_G
Cuts at: 0 61 85 181 882 922 1275 1809 1890
Size: 61 24 96 701 40 353 534 81
Fragments arranged by size:
701 534 353 96 81 61 40 24
SmaI CCC'GGG
Cuts at: 0 1540 1890
Size: 1540 350
SmlI C'TyrA_G
Cuts at: 0 1018 1890
Size: 1018 872
SphI G_CATG'C
Cuts at: 0 96 1890
Size: 96 1794
SrfI GCCC'GGGC
Cuts at: 0 1540 1890
Size: 1540 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 213 862 1890
Size: 213 649 1028
Fragments arranged by size:
1028 649 213
TatI w'GTAC_w
Cuts at: 0 233 369 1890
Size: 233 136 1521
Fragments arranged by size:
1521 233 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 848 880 1117 1149 1890
Size: 848 32 237 32 741
Fragments arranged by size:
848 741 237 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1063 1890
Size: 1063 827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1494 1890
Size: 1494 396
XmaI C'CCGG_G
Cuts at: 0 1538 1890
Size: 1538 352
XmnI GAAnn'nnTTC
Cuts at: 0 1148 1210 1890
Size: 1148 62 680
Fragments arranged by size:
1148 680 62
ZraI GAC'GTC
Cuts at: 0 707 1890
Size: 707 1183
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AvaI BanI BanII
BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssSI BstAPI BstYI BtgI BtsI CspCI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII HindIII MfeI MluI MmeI MscI MslI
MspA1I NspI PciI PflMI PfoI PpiI PspOMI PstI
PvuII SacI SapI ScaI SfcI SmaI SmlI SphI
SrfI TaqII TatI TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AhdI AloI AscI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglII BmtI BplI BsaBI BsiEI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI DraI EagI EcoRI EcoRV FalI
FseI FspI FspAI HpaI KasI KpnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SspI StuI StyI
SwaI XbaI XhoI