Restriction map for
pFN21AE5727
(Linear) (Six-base) MAPSORT of: KIEE5727.seq Check: 3256 from: 1 to: 2121
KIEE5727.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 473 1370 2121
Size: 473 897 751
Fragments arranged by size:
897 751 473
AatII G_ACGT'C
Cuts at: 0 302 2063 2121
Size: 302 1761 58
Fragments arranged by size:
1761 302 58
AccI GT'mk_AC
Cuts at: 0 1391 2121
Size: 1391 730
Acc65I G'GTAC_C
Cuts at: 0 85 2121
Size: 85 2036
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1479 1489 2121
Size: 1479 10 632
Fragments arranged by size:
1479 632 10
AfeI AGC'GCT
Cuts at: 0 406 1297 2121
Size: 406 891 824
Fragments arranged by size:
891 824 406
AflII C'TTAA_G
Cuts at: 0 454 2121
Size: 454 1667
AflIII A'CryG_T
Cuts at: 0 1941 2121
Size: 1941 180
AgeI A'CCGG_T
Cuts at: 0 75 2121
Size: 75 2046
AleI CACnn'nnGTG
Cuts at: 0 31 1803 2121
Size: 31 1772 318
Fragments arranged by size:
1772 318 31
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1378 2121
Size: 1378 743
AlwNI CAG_nnn'CTG
Cuts at: 0 243 553 1584 1971 2121
Size: 243 310 1031 387 150
Fragments arranged by size:
1031 387 310 243 150
ApaI G_GGCC'C
Cuts at: 0 643 1155 2121
Size: 643 512 966
Fragments arranged by size:
966 643 512
AvaI C'yCGr_G
Cuts at: 0 127 794 1015 1291 1320 1626 1995 2089
Size: 127 667 221 276 29 306 369 94
Cuts at: 2089 2121
Size: 32
Fragments arranged by size:
667 369 306 276 221 127 94 32
29
BamHI G'GATC_C
Cuts at: 0 2031 2121
Size: 2031 90
BanI G'GyrC_C
Cuts at: 0 78 85 502 538 585 1010 1663 1999
Size: 78 7 417 36 47 425 653 336
Cuts at: 1999 2121
Size: 122
Fragments arranged by size:
653 425 417 336 122 78 47 36
7
BanII G_rGCy'C
Cuts at: 0 128 187 643 1155 1763 2009 2121
Size: 128 59 456 512 608 246 112
Fragments arranged by size:
608 512 456 246 128 112 59
BbeI G_GCGC'C
Cuts at: 0 506 542 589 1014 2121
Size: 506 36 47 425 1107
Fragments arranged by size:
1107 506 425 47 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1579 1613 2121
Size: 1579 34 508
Fragments arranged by size:
1579 508 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1484 2121
Size: 1484 637
BglI GCCn_nnn'nGGC
Cuts at: 0 1651 1961 2121
Size: 1651 310 160
Fragments arranged by size:
1651 310 160
BglII A'GATC_T
Cuts at: 0 275 2121
Size: 275 1846
BlpI GC'TnA_GC
Cuts at: 0 876 2121
Size: 876 1245
Bme1580I G_kGCm'C
Cuts at: 0 643 774 809 1155 2121
Size: 643 131 35 346 966
Fragments arranged by size:
966 643 346 131 35
BmgBI CAC'GTC
Cuts at: 0 2055 2121
Size: 2055 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 985 1503 2121
Size: 985 518 618
Fragments arranged by size:
985 618 518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 447 1954 2121
Size: 214 233 1507 167
Fragments arranged by size:
1507 233 214 167
Bpu10I CC'TnA_GC
Cuts at: 0 1519 2121
Size: 1519 602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1747 1786 2121
Size: 1747 39 335
Fragments arranged by size:
1747 335 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 571 1333 2121
Size: 571 762 788
Fragments arranged by size:
788 762 571
BsaAI yAC'GTr
Cuts at: 0 1212 2121
Size: 1212 909
BsaHI Gr'CG_yC
Cuts at: 0 299 503 539 586 1011 1195 1617 2060
Size: 299 204 36 47 425 184 422 443
Cuts at: 2060 2121
Size: 61
Fragments arranged by size:
443 425 422 299 204 184 61 47
36
BsaWI w'CCGG_w
Cuts at: 0 75 1476 2121
Size: 75 1401 645
Fragments arranged by size:
1401 645 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 360 390 2121
Size: 360 30 1731
Fragments arranged by size:
1731 360 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 415 822 955 2121
Size: 328 87 407 133 1166
Fragments arranged by size:
1166 407 328 133 87
BseYI C'CCAG_C
Cuts at: 0 96 546 987 1105 1533 1837 2121
Size: 96 450 441 118 428 304 284
Fragments arranged by size:
450 441 428 304 284 118 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1374 1849 2121
Size: 496 878 475 272
Fragments arranged by size:
878 496 475 272
BsiEI CG_ry'CG
Cuts at: 0 59 917 1514 1557 1646 2121
Size: 59 858 597 43 89 475
Fragments arranged by size:
858 597 475 89 59 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 290 2121
Size: 290 1831
Bsp1286I G_dGCh'C
Cuts at: 0 128 187 643 774 809 1155 1763 2009
Size: 128 59 456 131 35 346 608 246
Cuts at: 2009 2121
Size: 112
Fragments arranged by size:
608 456 346 246 131 128 112 59
35
BspEI T'CCGG_A
Cuts at: 0 1476 2121
Size: 1476 645
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 473 1370 1687 2121
Size: 473 897 317 434
Fragments arranged by size:
897 473 434 317
BsrBI CCG'CTC
Cuts at: 0 785 919 1022 1443 2121
Size: 785 134 103 421 678
Fragments arranged by size:
785 678 421 134 103
BsrDI GCAATG_nn'
Cuts at: 0 289 2121
Size: 289 1832
BsrFI r'CCGG_y
Cuts at: 0 58 75 892 964 1377 1419 1641 1711
Size: 58 17 817 72 413 42 222 70
Cuts at: 1711 2121
Size: 410
Fragments arranged by size:
817 413 410 222 72 70 58 42
17
BssHII G'CGCG_C
Cuts at: 0 1218 1301 2121
Size: 1218 83 820
Fragments arranged by size:
1218 820 83
BstEII G'GTnAC_C
Cuts at: 0 1051 2121
Size: 1051 1070
BstXI CCAn_nnnn'nTGG
Cuts at: 0 731 2121
Size: 731 1390
BstYI r'GATC_y
Cuts at: 0 275 1766 2031 2121
Size: 275 1491 265 90
Fragments arranged by size:
1491 275 265 90
BtsI GCAGTG_nn'
Cuts at: 0 474 2121
Size: 474 1647
DraIII CAC_nnn'GTG
Cuts at: 0 480 2121
Size: 480 1641
DrdI GACnn_nn'nnGTC
Cuts at: 0 2059 2121
Size: 2059 62
EaeI y'GGCC_r
Cuts at: 0 56 707 914 1421 1511 1544 1643 1713
Size: 56 651 207 507 90 33 99 70
Cuts at: 1713 1962 2121
Size: 249 159
Fragments arranged by size:
651 507 249 207 159 99 90 70
56 33
EagI C'GGCC_G
Cuts at: 0 56 914 1511 1643 2121
Size: 56 858 597 132 478
Fragments arranged by size:
858 597 478 132 56
EarI CTCTTCn'nnn_
Cuts at: 0 699 1184 2121
Size: 699 485 937
Fragments arranged by size:
937 699 485
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1188 2121
Size: 1188 933
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 447 1479 1489 1954 2121
Size: 214 233 1032 10 465 167
Fragments arranged by size:
1032 465 233 214 167 10
EcoO109I rG'GnC_Cy
Cuts at: 0 1110 1151 1152 1929 2121
Size: 1110 41 1 777 192
Fragments arranged by size:
1110 777 192 41 1
FspI TGC'GCA
Cuts at: 0 148 1262 2121
Size: 148 1114 859
Fragments arranged by size:
1114 859 148
FspAI rTGC'GCAy
Cuts at: 0 148 2121
Size: 148 1973
HaeII r_GCGC'y
Cuts at: 0 22 408 506 542 589 1014 1299 2121
Size: 22 386 98 36 47 425 285 822
Fragments arranged by size:
822 425 386 285 98 47 36 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 260 292 366 398 1522 1554 2121
Size: 260 32 74 32 1124 32 567
Fragments arranged by size:
1124 567 260 74 32 32 32
KasI G'GCGC_C
Cuts at: 0 502 538 585 1010 2121
Size: 502 36 47 425 1111
Fragments arranged by size:
1111 502 425 47 36
KpnI G_GTAC'C
Cuts at: 0 89 2121
Size: 89 2032
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 2121
Size: 403 1718
MscI TGG'CCA
Cuts at: 0 1546 1964 2121
Size: 1546 418 157
Fragments arranged by size:
1546 418 157
MslI CAynn'nnrTG
Cuts at: 0 31 1803 2121
Size: 31 1772 318
Fragments arranged by size:
1772 318 31
MspA1I CmG'CkG
Cuts at: 0 488 550 762 1266 1499 1510 1584 1682
Size: 488 62 212 504 233 11 74 98
Cuts at: 1682 1691 1892 2121
Size: 9 201 229
Fragments arranged by size:
504 488 233 229 212 201 98 74
62 11 9
NaeI GCC'GGC
Cuts at: 0 60 894 966 1379 2121
Size: 60 834 72 413 742
Fragments arranged by size:
834 742 413 72 60
NarI GG'CG_CC
Cuts at: 0 503 539 586 1011 2121
Size: 503 36 47 425 1110
Fragments arranged by size:
1110 503 425 47 36
NgoMIV G'CCGG_C
Cuts at: 0 58 892 964 1377 2121
Size: 58 834 72 413 744
Fragments arranged by size:
834 744 413 72 58
NotI GC'GGCC_GC
Cuts at: 0 914 1511 2121
Size: 914 597 610
Fragments arranged by size:
914 610 597
NspI r_CATG'y
Cuts at: 0 1776 1945 2121
Size: 1776 169 176
Fragments arranged by size:
1776 176 169
PasI CC'CwG_GG
Cuts at: 0 636 2121
Size: 636 1485
PciI A'CATG_T
Cuts at: 0 1941 2121
Size: 1941 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 636 2121
Size: 636 1485
PmlI CAC'GTG
Cuts at: 0 1212 2121
Size: 1212 909
PpuMI rG'GwC_Cy
Cuts at: 0 1110 1929 2121
Size: 1110 819 192
Fragments arranged by size:
1110 819 192
PspOMI G'GGCC_C
Cuts at: 0 639 1151 2121
Size: 639 512 970
Fragments arranged by size:
970 639 512
PspXI vC'TCGA_Gb
Cuts at: 0 1291 2121
Size: 1291 830
PstI C_TGCA'G
Cuts at: 0 1509 2121
Size: 1509 612
PvuII CAG'CTG
Cuts at: 0 762 1266 1584 2121
Size: 762 504 318 537
Fragments arranged by size:
762 537 504 318
RsrII CG'GwC_CG
Cuts at: 0 73 2121
Size: 73 2048
SanDI GG'GwC_CC
Cuts at: 0 1110 2121
Size: 1110 1011
SfcI C'TryA_G
Cuts at: 0 1505 2121
Size: 1505 616
SfoI GGC'GCC
Cuts at: 0 504 540 587 1012 2121
Size: 504 36 47 425 1109
Fragments arranged by size:
1109 504 425 47 36
SmaI CCC'GGG
Cuts at: 0 129 1017 1322 1997 2121
Size: 129 888 305 675 124
Fragments arranged by size:
888 675 305 129 124
SmlI C'TyrA_G
Cuts at: 0 454 1291 1762 1801 2121
Size: 454 837 471 39 320
Fragments arranged by size:
837 471 454 320 39
SrfI GCCC'GGGC
Cuts at: 0 129 2121
Size: 129 1992
StuI AGG'CCT
Cuts at: 0 658 691 2121
Size: 658 33 1430
Fragments arranged by size:
1430 658 33
StyI C'CwwG_G
Cuts at: 0 341 534 2121
Size: 341 193 1587
Fragments arranged by size:
1587 341 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 553 1571 2121
Size: 553 1018 550
Fragments arranged by size:
1018 553 550
TatI w'GTAC_w
Cuts at: 0 1742 2121
Size: 1742 379
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 360 392 2121
Size: 360 32 1729
Fragments arranged by size:
1729 360 32
XhoI C'TCGA_G
Cuts at: 0 1291 2121
Size: 1291 830
XmaI C'CCGG_G
Cuts at: 0 127 1015 1320 1995 2121
Size: 127 888 305 675 126
Fragments arranged by size:
888 675 305 127 126
ZraI GAC'GTC
Cuts at: 0 300 2061 2121
Size: 300 1761 60
Fragments arranged by size:
1761 300 60
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AleI AlfI AlwNI ApaI AvaI BamHI BanI
BanII BbeI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssHII BstEII BstXI
BstYI BtsI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I FspI FspAI HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI
NspI PasI PciI PflMI PmlI PpuMI PspOMI PspXI
PstI PvuII RsrII SanDI SfcI SfoI SmaI SmlI
SrfI StuI StyI TaqII TatI TstI XhoI XmaI
ZraI
Enzymes that do not cut:
AclI AhdI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbsI BbvCI BclI BfrBI BmrI BmtI
BsaBI BsiHKAI BsiWI BsmI BspHI BsrGI BssSI BstAPI
BstBI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
EcoICRI EcoNI EcoRI EcoRV FalI FseI HincII HindIII
HpaI MfeI MluI NcoI NdeI NheI NruI NsiI
PacI PfoI PmeI PpiI PshAI PsiI PsrI PvuI
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I
XbaI XcmI XmnI