Restriction map for
pFN21AE5723
(Linear) (Six-base) MAPSORT of: KIEE5723.seq Check: 6693 from: 1 to: 2442
KIEE5723.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 1505 2442
Size: 68 1437 937
Fragments arranged by size:
1437 937 68
AflIII A'CryG_T
Cuts at: 0 117 2103 2442
Size: 117 1986 339
Fragments arranged by size:
1986 339 117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2157 2442
Size: 2157 285
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2165 2197 2442
Size: 2165 32 245
Fragments arranged by size:
2165 245 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1528 2442
Size: 1528 914
ApaLI G'TGCA_C
Cuts at: 0 2405 2442
Size: 2405 37
ApoI r'AATT_y
Cuts at: 0 555 579 1428 2442
Size: 555 24 849 1014
Fragments arranged by size:
1014 849 555 24
AseI AT'TA_AT
Cuts at: 0 353 2442
Size: 353 2089
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1933 1966 2442
Size: 1933 33 476
Fragments arranged by size:
1933 476 33
BamHI G'GATC_C
Cuts at: 0 1573 2442
Size: 1573 869
BanI G'GyrC_C
Cuts at: 0 846 2442
Size: 846 1596
BanII G_rGCy'C
Cuts at: 0 338 2442
Size: 338 2104
BbsI GAAGACnn'nnnn_
Cuts at: 0 83 2077 2442
Size: 83 1994 365
Fragments arranged by size:
1994 365 83
BbvCI CC'TCA_GC
Cuts at: 0 756 2442
Size: 756 1686
Bme1580I G_kGCm'C
Cuts at: 0 2409 2442
Size: 2409 33
BmgBI CAC'GTC
Cuts at: 0 1441 2442
Size: 1441 1001
BmrI ACTGGGnnnn_n'
Cuts at: 0 1559 2442
Size: 1559 883
BmtI G_CTAG'C
Cuts at: 0 947 2442
Size: 947 1495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 1373 1727 2253 2442
Size: 433 940 354 526 189
Fragments arranged by size:
940 526 433 354 189
Bpu10I CC'TnA_GC
Cuts at: 0 756 2442
Size: 756 1686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 667 2100 2442
Size: 241 426 1433 342
Fragments arranged by size:
1433 426 342 241
BsaI GGTCTCn'nnnn_
Cuts at: 0 2197 2442
Size: 2197 245
BsaBI GATnn'nnATC
Cuts at: 0 1226 2442
Size: 1226 1216
BsaWI w'CCGG_w
Cuts at: 0 1576 2442
Size: 1576 866
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1346 1376 2165 2195 2442
Size: 1346 30 789 30 247
Fragments arranged by size:
1346 789 247 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1603 2442
Size: 1603 839
BsiHKAI G_wGCw'C
Cuts at: 0 364 2400 2409 2442
Size: 364 2036 9 33
Fragments arranged by size:
2036 364 33 9
BsmI GAATG_Cn'
Cuts at: 0 947 1287 2166 2442
Size: 947 340 879 276
Fragments arranged by size:
947 879 340 276
Bsp1286I G_dGCh'C
Cuts at: 0 338 364 2400 2409 2442
Size: 338 26 2036 9 33
Fragments arranged by size:
2036 338 33 26 9
BspHI T'CATG_A
Cuts at: 0 69 494 1224 1320 1424 2442
Size: 69 425 730 96 104 1018
Fragments arranged by size:
1018 730 425 104 96 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1606 2442
Size: 1606 836
BsrDI GCAATG_nn'
Cuts at: 0 1248 1503 2442
Size: 1248 255 939
Fragments arranged by size:
1248 939 255
BsrGI T'GTAC_A
Cuts at: 0 1628 2442
Size: 1628 814
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2404 2442
Size: 2404 38
BstXI CCAn_nnnn'nTGG
Cuts at: 0 277 2442
Size: 277 2165
BstYI r'GATC_y
Cuts at: 0 12 1055 1122 1561 1573 2442
Size: 12 1043 67 439 12 869
Fragments arranged by size:
1043 869 439 67 12 12
BtgI C'CryG_G
Cuts at: 0 1112 2442
Size: 1112 1330
BtsI GCAGTG_nn'
Cuts at: 0 917 2240 2442
Size: 917 1323 202
Fragments arranged by size:
1323 917 202
ClaI AT'CG_AT
Cuts at: 0 1230 2442
Size: 1230 1212
DraI TTT'AAA
Cuts at: 0 197 858 2442
Size: 197 661 1584
Fragments arranged by size:
1584 661 197
DraIII CAC_nnn'GTG
Cuts at: 0 2404 2442
Size: 2404 38
EarI CTCTTCn'nnn_
Cuts at: 0 87 2442
Size: 87 2355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 433 1373 1505 1727 2253 2442
Size: 68 365 940 132 222 526 189
Fragments arranged by size:
940 526 365 222 189 132 68
EcoRI G'AATT_C
Cuts at: 0 1428 2442
Size: 1428 1014
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 282 823 2434 2442
Size: 282 541 1611 8
Fragments arranged by size:
1611 541 282 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 621 653 1346 1378 1806 1838
Size: 64 32 525 32 693 32 428 32
Cuts at: 1838 2320 2352 2442
Size: 482 32 90
Fragments arranged by size:
693 525 482 428 90 64 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1312 2442
Size: 1312 1130
HindIII A'AGCT_T
Cuts at: 0 23 1912 2442
Size: 23 1889 530
Fragments arranged by size:
1889 530 23
MfeI C'AATT_G
Cuts at: 0 2014 2442
Size: 2014 428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 2442
Size: 40 2402
MslI CAynn'nnrTG
Cuts at: 0 1084 2360 2442
Size: 1084 1276 82
Fragments arranged by size:
1276 1084 82
MspA1I CmG'CkG
Cuts at: 0 711 2038 2442
Size: 711 1327 404
Fragments arranged by size:
1327 711 404
NdeI CA'TA_TG
Cuts at: 0 1017 2442
Size: 1017 1425
NheI G'CTAG_C
Cuts at: 0 943 2442
Size: 943 1499
NspI r_CATG'y
Cuts at: 0 121 460 2107 2442
Size: 121 339 1647 335
Fragments arranged by size:
1647 339 335 121
PciI A'CATG_T
Cuts at: 0 117 2103 2442
Size: 117 1986 339
Fragments arranged by size:
1986 339 117
PfoI T'CCnGG_A
Cuts at: 0 2269 2442
Size: 2269 173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2165 2197 2442
Size: 2165 32 245
Fragments arranged by size:
2165 245 32
PsiI TTA'TAA
Cuts at: 0 349 2442
Size: 349 2093
PvuII CAG'CTG
Cuts at: 0 711 2038 2442
Size: 711 1327 404
Fragments arranged by size:
1327 711 404
ScaI AGT'ACT
Cuts at: 0 230 2442
Size: 230 2212
SfcI C'TryA_G
Cuts at: 0 98 1250 2442
Size: 98 1152 1192
Fragments arranged by size:
1192 1152 98
SmlI C'TyrA_G
Cuts at: 0 256 682 2079 2442
Size: 256 426 1397 363
Fragments arranged by size:
1397 426 363 256
SspI AAT'ATT
Cuts at: 0 1260 1746 2442
Size: 1260 486 696
Fragments arranged by size:
1260 696 486
StuI AGG'CCT
Cuts at: 0 57 741 2442
Size: 57 684 1701
Fragments arranged by size:
1701 684 57
StyI C'CwwG_G
Cuts at: 0 1634 2442
Size: 1634 808
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1340 2442
Size: 1340 1102
TatI w'GTAC_w
Cuts at: 0 228 673 1628 1930 2442
Size: 228 445 955 302 512
Fragments arranged by size:
955 512 445 302 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1565 1597 2442
Size: 1565 32 845
Fragments arranged by size:
1565 845 32
XbaI T'CTAG_A
Cuts at: 0 487 2442
Size: 487 1955
XmnI GAAnn'nnTTC
Cuts at: 0 1259 2442
Size: 1259 1183
Enzymes that do cut:
AcuI AflIII AlfI AloI AlwNI ApaLI ApoI AseI
BaeI BamHI BanI BanII BbsI BbvCI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI
BsaXI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrGI BstAPI BstXI BstYI BtgI BtsI ClaI DraI
DraIII EarI Eco57MI EcoRI FalI Hin4I HincII HindIII
MfeI MmeI MslI MspA1I NdeI NheI NspI PciI
PfoI PpiI PsiI PvuII ScaI SfcI SmlI SspI
StuI StyI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI ApaI AscI AsiSI AvaI AvrII BbeI
BcgI BciVI BclI BfrBI BglI BglII BlpI BplI
BsaAI BsaHI BseRI BseYI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI CspCI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FseI FspI FspAI HaeII HpaI KasI
KpnI MluI MscI NaeI NarI NcoI NgoMIV NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
Tth111I XcmI XhoI XmaI ZraI