Restriction map for
pFN21AE5718
(Linear) (Six-base) MAPSORT of: KIEE5718.seq Check: 9153 from: 1 to: 2913
KIEE5718.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AccI GT'mk_AC
Cuts at: 0 2311 2913
Size: 2311 602
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 355 748 903 1148 2746 2913
Size: 91 264 393 155 245 1598 167
Fragments arranged by size:
1598 393 264 245 167 155 91
AflII C'TTAA_G
Cuts at: 0 1551 2913
Size: 1551 1362
AhdI GACnn_n'nnGTC
Cuts at: 0 11 2913
Size: 11 2902
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1241 1273 2077 2109 2913
Size: 1241 32 804 32 804
Fragments arranged by size:
1241 804 804 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1773 1795 2913
Size: 1773 22 1118
Fragments arranged by size:
1773 1118 22
ApoI r'AATT_y
Cuts at: 0 109 488 869 878 1293 1576 2913
Size: 109 379 381 9 415 283 1337
Fragments arranged by size:
1337 415 381 379 283 109 9
BamHI G'GATC_C
Cuts at: 0 2074 2521 2913
Size: 2074 447 392
Fragments arranged by size:
2074 447 392
BanI G'GyrC_C
Cuts at: 0 1287 1690 2913
Size: 1287 403 1223
Fragments arranged by size:
1287 1223 403
BanII G_rGCy'C
Cuts at: 0 516 734 2321 2913
Size: 516 218 1587 592
Fragments arranged by size:
1587 592 516 218
BciVI GTATCCnnnnn_n'
Cuts at: 0 974 2913
Size: 974 1939
BfrBI ATG'CAT
Cuts at: 0 1802 2913
Size: 1802 1111
BglI GCCn_nnn'nGGC
Cuts at: 0 2041 2913
Size: 2041 872
BglII A'GATC_T
Cuts at: 0 1005 1835 2244 2913
Size: 1005 830 409 669
Fragments arranged by size:
1005 830 669 409
BlpI GC'TnA_GC
Cuts at: 0 2771 2913
Size: 2771 142
Bme1580I G_kGCm'C
Cuts at: 0 1695 2913
Size: 1695 1218
BmrI ACTGGGnnnn_n'
Cuts at: 0 1830 2913
Size: 1830 1083
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1868 2913
Size: 1868 1045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 1636 2913
Size: 895 741 1277
Fragments arranged by size:
1277 895 741
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1173 2269 2913
Size: 1173 1096 644
Fragments arranged by size:
1173 1096 644
BsaWI w'CCGG_w
Cuts at: 0 2071 2913
Size: 2071 842
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 278 308 614 644 1612 1642 2077 2107
Size: 278 30 306 30 968 30 435 30
Cuts at: 2107 2913
Size: 806
Fragments arranged by size:
968 806 435 306 278 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 158 1633 1871 2101 2104 2377 2878 2913
Size: 158 1475 238 230 3 273 501 35
Fragments arranged by size:
1475 501 273 238 230 158 35 3
BseYI C'CCAG_C
Cuts at: 0 457 2913
Size: 457 2456
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1403 2913
Size: 43 1360 1510
Fragments arranged by size:
1510 1360 43
BsiHKAI G_wGCw'C
Cuts at: 0 2321 2913
Size: 2321 592
BsmI GAATG_Cn'
Cuts at: 0 92 1804 2058 2295 2913
Size: 92 1712 254 237 618
Fragments arranged by size:
1712 618 254 237 92
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1673 2913
Size: 1673 1240
Bsp1286I G_dGCh'C
Cuts at: 0 516 734 1695 2321 2913
Size: 516 218 961 626 592
Fragments arranged by size:
961 626 592 516 218
BspHI T'CATG_A
Cuts at: 0 84 2913
Size: 84 2829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 2913
Size: 74 2839
BsrBI CCG'CTC
Cuts at: 0 611 2913
Size: 611 2302
BsrDI GCAATG_nn'
Cuts at: 0 124 2913
Size: 124 2789
BssSI C'ACGA_G
Cuts at: 0 349 1783 2913
Size: 349 1434 1130
Fragments arranged by size:
1434 1130 349
BstBI TT'CG_AA
Cuts at: 0 882 2913
Size: 882 2031
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1035 2913
Size: 1035 1878
BstYI r'GATC_y
Cuts at: 0 1005 1835 2074 2244 2521 2913
Size: 1005 830 239 170 277 392
Fragments arranged by size:
1005 830 392 277 239 170
BtgI C'CryG_G
Cuts at: 0 133 152 411 588 2030 2913
Size: 133 19 259 177 1442 883
Fragments arranged by size:
1442 883 259 177 133 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1248 2913
Size: 1248 1665
BtsI GCAGTG_nn'
Cuts at: 0 207 2913
Size: 207 2706
ClaI AT'CG_AT
Cuts at: 0 938 2913
Size: 938 1975
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 337 372 2913
Size: 337 35 2541
Fragments arranged by size:
2541 337 35
DraI TTT'AAA
Cuts at: 0 338 2913
Size: 338 2575
DraIII CAC_nnn'GTG
Cuts at: 0 1284 2913
Size: 1284 1629
DrdI GACnn_nn'nnGTC
Cuts at: 0 798 2913
Size: 798 2115
EaeI y'GGCC_r
Cuts at: 0 1811 2033 2913
Size: 1811 222 880
Fragments arranged by size:
1811 880 222
EarI CTCTTCn'nnn_
Cuts at: 0 245 803 1124 1591 2326 2722 2913
Size: 245 558 321 467 735 396 191
Fragments arranged by size:
735 558 467 396 321 245 191
EcoICRI GAG'CTC
Cuts at: 0 2319 2913
Size: 2319 594
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 355 748 895 903 1148 1636 2746
Size: 91 264 393 147 8 245 488 1110
Cuts at: 2746 2913
Size: 167
Fragments arranged by size:
1110 488 393 264 245 167 147 91
8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 70 2913
Size: 70 2843
EcoO109I rG'GnC_Cy
Cuts at: 0 2874 2913
Size: 2874 39
EcoRI G'AATT_C
Cuts at: 0 109 488 869 1576 2913
Size: 109 379 381 707 1337
Fragments arranged by size:
1337 707 381 379 109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2791 2913
Size: 2791 122
FspI TGC'GCA
Cuts at: 0 934 2913
Size: 934 1979
FspAI rTGC'GCAy
Cuts at: 0 934 2913
Size: 934 1979
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 464 496 585 617 802 834 940
Size: 29 435 32 89 32 185 32 106
Cuts at: 940 972 1201 1233 1438 1470 1936 1968 2557
Size: 32 229 32 205 32 466 32 589
Cuts at: 2557 2589 2913
Size: 32 324
Fragments arranged by size:
589 466 435 324 229 205 185 106
89 32 32 32 32 32 32 32
32 29
HincII GTy'rAC
Cuts at: 0 501 953 2913
Size: 501 452 1960
Fragments arranged by size:
1960 501 452
HindIII A'AGCT_T
Cuts at: 0 524 2913
Size: 524 2389
MfeI C'AATT_G
Cuts at: 0 227 1634 2913
Size: 227 1407 1279
Fragments arranged by size:
1407 1279 227
MscI TGG'CCA
Cuts at: 0 1813 2035 2913
Size: 1813 222 878
Fragments arranged by size:
1813 878 222
MslI CAynn'nnrTG
Cuts at: 0 623 1033 2913
Size: 623 410 1880
Fragments arranged by size:
1880 623 410
MspA1I CmG'CkG
Cuts at: 0 190 2913
Size: 190 2723
NcoI C'CATG_G
Cuts at: 0 152 411 2030 2913
Size: 152 259 1619 883
Fragments arranged by size:
1619 883 259 152
NsiI A_TGCA'T
Cuts at: 0 1804 2913
Size: 1804 1109
NspI r_CATG'y
Cuts at: 0 1365 2334 2913
Size: 1365 969 579
Fragments arranged by size:
1365 969 579
PflMI CCAn_nnn'nTGG
Cuts at: 0 1820 2434 2913
Size: 1820 614 479
Fragments arranged by size:
1820 614 479
PfoI T'CCnGG_A
Cuts at: 0 911 1910 2885 2913
Size: 911 999 975 28
Fragments arranged by size:
999 975 911 28
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2077 2109 2913
Size: 2077 32 804
Fragments arranged by size:
2077 804 32
PpuMI rG'GwC_Cy
Cuts at: 0 2874 2913
Size: 2874 39
PvuII CAG'CTG
Cuts at: 0 190 2913
Size: 190 2723
SacI G_AGCT'C
Cuts at: 0 2321 2913
Size: 2321 592
SapI GCTCTTCn'nnn_
Cuts at: 0 1591 2326 2913
Size: 1591 735 587
Fragments arranged by size:
1591 735 587
SfcI C'TryA_G
Cuts at: 0 740 2913
Size: 740 2173
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2041 2913
Size: 2041 872
SmlI C'TyrA_G
Cuts at: 0 1152 1551 2248 2913
Size: 1152 399 697 665
Fragments arranged by size:
1152 697 665 399
StuI AGG'CCT
Cuts at: 0 1861 2626 2913
Size: 1861 765 287
Fragments arranged by size:
1861 765 287
StyI C'CwwG_G
Cuts at: 0 152 411 856 2030 2428 2913
Size: 152 259 445 1174 398 485
Fragments arranged by size:
1174 485 445 398 259 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1663 2913
Size: 1663 1250
TatI w'GTAC_w
Cuts at: 0 2060 2169 2333 2913
Size: 2060 109 164 580
Fragments arranged by size:
2060 580 164 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 278 310 965 997 1612 1644 2430 2462
Size: 278 32 655 32 615 32 786 32
Cuts at: 2462 2913
Size: 451
Fragments arranged by size:
786 655 615 451 278 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 2859 2913
Size: 2859 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1109 1770 1962 2913
Size: 1109 661 192 951
Fragments arranged by size:
1109 951 661 192
XmnI GAAnn'nnTTC
Cuts at: 0 434 2154 2702 2913
Size: 434 1720 548 211
Fragments arranged by size:
1720 548 434 211
Enzymes that do cut:
AccI AcuI AflII AhdI AloI AlwNI ApoI BamHI
BanI BanII BciVI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BssSI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI Hin4I
HincII HindIII MfeI MscI MslI MspA1I NcoI NsiI
NspI PflMI PfoI PpiI PpuMI PvuII SacI SapI
SfcI SfiI SmlI StuI StyI TaqII TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbsI BbvCI BcgI BclI BmgBI BmtI
Bpu10I BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BspEI
BsrFI BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I EagI
EciI EcoRV FseI HaeII HpaI KasI KpnI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XhoI XmaI
ZraI