Restriction map for
pFN21AE5716
(Linear) (Six-base) MAPSORT of: KIEE5716.seq Check: 1862 from: 1 to: 1821
KIEE5716.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1382 1636 1821
Size: 1382 254 185
Fragments arranged by size:
1382 254 185
AatII G_ACGT'C
Cuts at: 0 396 1821
Size: 396 1425
AfeI AGC'GCT
Cuts at: 0 1063 1821
Size: 1063 758
AhdI GACnn_n'nnGTC
Cuts at: 0 159 1821
Size: 159 1662
AleI CACnn'nnGTG
Cuts at: 0 1327 1821
Size: 1327 494
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 437 469 1821
Size: 437 32 1352
Fragments arranged by size:
1352 437 32
AlwNI CAG_nnn'CTG
Cuts at: 0 210 870 1281 1365 1390 1688 1821
Size: 210 660 411 84 25 298 133
Fragments arranged by size:
660 411 298 210 133 84 25
ApaI G_GGCC'C
Cuts at: 0 852 1821
Size: 852 969
ApoI r'AATT_y
Cuts at: 0 606 1349 1821
Size: 606 743 472
Fragments arranged by size:
743 606 472
AvaI C'yCGr_G
Cuts at: 0 299 773 845 935 1173 1821
Size: 299 474 72 90 238 648
Fragments arranged by size:
648 474 299 238 90 72
AvrII C'CTAG_G
Cuts at: 0 1264 1821
Size: 1264 557
BamHI G'GATC_C
Cuts at: 0 795 1821
Size: 795 1026
BanI G'GyrC_C
Cuts at: 0 1582 1821
Size: 1582 239
BanII G_rGCy'C
Cuts at: 0 852 1319 1821
Size: 852 467 502
Fragments arranged by size:
852 502 467
BbvCI CC'TCA_GC
Cuts at: 0 1391 1401 1540 1821
Size: 1391 10 139 281
Fragments arranged by size:
1391 281 139 10
BclI T'GATC_A
Cuts at: 0 1127 1821
Size: 1127 694
BglI GCCn_nnn'nGGC
Cuts at: 0 1477 1781 1821
Size: 1477 304 40
Fragments arranged by size:
1477 304 40
BlpI GC'TnA_GC
Cuts at: 0 1678 1821
Size: 1678 143
Bme1580I G_kGCm'C
Cuts at: 0 852 1821
Size: 852 969
BmgBI CAC'GTC
Cuts at: 0 750 1821
Size: 750 1071
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1416 1821
Size: 1416 405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 544 659 1149 1748 1821
Size: 78 466 115 490 599 73
Fragments arranged by size:
599 490 466 115 78 73
Bpu10I CC'TnA_GC
Cuts at: 0 1391 1401 1540 1821
Size: 1391 10 139 281
Fragments arranged by size:
1391 281 139 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 930 1821
Size: 930 891
BsaI GGTCTCn'nnnn_
Cuts at: 0 1597 1741 1821
Size: 1597 144 80
Fragments arranged by size:
1597 144 80
BsaHI Gr'CG_yC
Cuts at: 0 393 1821
Size: 393 1428
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1327 1357 1746 1776 1821
Size: 1327 30 389 30 45
Fragments arranged by size:
1327 389 45 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 297 480 991 994 1003 1669 1672
Size: 286 11 183 511 3 9 666 3
Cuts at: 1672 1675 1708 1711 1720 1741 1759 1821
Size: 3 33 3 9 21 18 62
Fragments arranged by size:
666 511 286 183 62 33 21 18
11 9 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 210 740 946 999 1803 1821
Size: 210 530 206 53 804 18
Fragments arranged by size:
804 530 210 206 53 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1378 1821
Size: 1378 443
BsiEI CG_ry'CG
Cuts at: 0 1536 1821
Size: 1536 285
BsiHKAI G_wGCw'C
Cuts at: 0 518 1821
Size: 518 1303
Bsp1286I G_dGCh'C
Cuts at: 0 518 852 1319 1821
Size: 518 334 467 502
Fragments arranged by size:
518 502 467 334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1266 1382 1636 1821
Size: 1266 116 254 185
Fragments arranged by size:
1266 254 185 116
BsrBI CCG'CTC
Cuts at: 0 63 126 1821
Size: 63 63 1695
Fragments arranged by size:
1695 63 63
BsrDI GCAATG_nn'
Cuts at: 0 1040 1821
Size: 1040 781
BsrFI r'CCGG_y
Cuts at: 0 318 1821
Size: 318 1503
BsrGI T'GTAC_A
Cuts at: 0 1493 1821
Size: 1493 328
BssSI C'ACGA_G
Cuts at: 0 218 1821
Size: 218 1603
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1365 1821
Size: 1365 456
BstEII G'GTnAC_C
Cuts at: 0 1080 1821
Size: 1080 741
BstXI CCAn_nnnn'nTGG
Cuts at: 0 894 1614 1821
Size: 894 720 207
Fragments arranged by size:
894 720 207
BstYI r'GATC_y
Cuts at: 0 681 795 1289 1307 1821
Size: 681 114 494 18 514
Fragments arranged by size:
681 514 494 114 18
BtgI C'CryG_G
Cuts at: 0 1607 1821
Size: 1607 214
BtsI GCAGTG_nn'
Cuts at: 0 1322 1532 1821
Size: 1322 210 289
Fragments arranged by size:
1322 289 210
ClaI AT'CG_AT
Cuts at: 0 237 1821
Size: 237 1584
EaeI y'GGCC_r
Cuts at: 0 1533 1782 1821
Size: 1533 249 39
Fragments arranged by size:
1533 249 39
EagI C'GGCC_G
Cuts at: 0 1533 1821
Size: 1533 288
EarI CTCTTCn'nnn_
Cuts at: 0 61 811 980 1262 1661 1667 1821
Size: 61 750 169 282 399 6 154
Fragments arranged by size:
750 399 282 169 154 61 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1161 1821
Size: 1161 660
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 544 659 1149 1748 1821
Size: 78 466 115 490 599 73
Fragments arranged by size:
599 490 466 115 78 73
EcoO109I rG'GnC_Cy
Cuts at: 0 777 849 1362 1395 1821
Size: 777 72 513 33 426
Fragments arranged by size:
777 513 426 72 33
EcoRI G'AATT_C
Cuts at: 0 1349 1821
Size: 1349 472
EcoRV GAT'ATC
Cuts at: 0 588 1821
Size: 588 1233
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 481 1821
Size: 481 1340
HaeII r_GCGC'y
Cuts at: 0 1065 1821
Size: 1065 756
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 1821
Size: 145 32 1644
Fragments arranged by size:
1644 145 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 317 615 802 1821
Size: 125 192 298 187 1019
Fragments arranged by size:
1019 298 192 187 125
MslI CAynn'nnrTG
Cuts at: 0 892 1327 1821
Size: 892 435 494
Fragments arranged by size:
892 494 435
MspA1I CmG'CkG
Cuts at: 0 113 540 917 1405 1821
Size: 113 427 377 488 416
Fragments arranged by size:
488 427 416 377 113
PasI CC'CwG_GG
Cuts at: 0 852 1821
Size: 852 969
PfoI T'CCnGG_A
Cuts at: 0 350 1310 1821
Size: 350 960 511
Fragments arranged by size:
960 511 350
PpuMI rG'GwC_Cy
Cuts at: 0 777 1395 1821
Size: 777 618 426
Fragments arranged by size:
777 618 426
PspOMI G'GGCC_C
Cuts at: 0 848 1821
Size: 848 973
PvuII CAG'CTG
Cuts at: 0 540 917 1821
Size: 540 377 904
Fragments arranged by size:
904 540 377
SanDI GG'GwC_CC
Cuts at: 0 777 1821
Size: 777 1044
SapI GCTCTTCn'nnn_
Cuts at: 0 1667 1821
Size: 1667 154
SfcI C'TryA_G
Cuts at: 0 313 1566 1821
Size: 313 1253 255
Fragments arranged by size:
1253 313 255
SmaI CCC'GGG
Cuts at: 0 301 1175 1821
Size: 301 874 646
Fragments arranged by size:
874 646 301
SmlI C'TyrA_G
Cuts at: 0 909 1821
Size: 909 912
StuI AGG'CCT
Cuts at: 0 1241 1821
Size: 1241 580
StyI C'CwwG_G
Cuts at: 0 400 419 1264 1472 1821
Size: 400 19 845 208 349
Fragments arranged by size:
845 400 349 208 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 157 1397 1795 1821
Size: 157 1240 398 26
Fragments arranged by size:
1240 398 157 26
TatI w'GTAC_w
Cuts at: 0 1493 1821
Size: 1493 328
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 753 785 1821
Size: 753 32 1036
Fragments arranged by size:
1036 753 32
Tth111I GACn'n_nGTC
Cuts at: 0 568 1821
Size: 568 1253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 463 1092 1614 1821
Size: 463 629 522 207
Fragments arranged by size:
629 522 463 207
XmaI C'CCGG_G
Cuts at: 0 299 1173 1821
Size: 299 874 648
Fragments arranged by size:
874 648 299
XmnI GAAnn'nnTTC
Cuts at: 0 16 1821
Size: 16 1805
ZraI GAC'GTC
Cuts at: 0 394 1821
Size: 394 1427
Enzymes that do cut:
AarI AatII AfeI AhdI AleI AloI AlwNI ApaI
ApoI AvaI AvrII BamHI BanI BanII BbvCI BclI
BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI BtgI BtsI ClaI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I MmeI MslI MspA1I PasI PfoI PpuMI PspOMI
PvuII SanDI SapI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
ApaLI AscI AseI AsiSI BaeI BbeI BbsI BcgI
BciVI BfrBI BglII BmrI BmtI BsaAI BsaBI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BssHII BstBI BstZ17I
Bsu36I BtgZI CspCI DraI DraIII DrdI EcoICRI EcoNI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI