Restriction map for
pFN21AE5710
(Linear) (Six-base) MAPSORT of: KIEE5710.seq Check: 3256 from: 1 to: 2535
KIEE5710.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AclI AA'CG_TT
Cuts at: 0 118 2535
Size: 118 2417
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1174 1693 1702 1825 2535
Size: 1174 519 9 123 710
Fragments arranged by size:
1174 710 519 123 9
AflII C'TTAA_G
Cuts at: 0 301 2535
Size: 301 2234
AhdI GACnn_n'nnGTC
Cuts at: 0 2325 2535
Size: 2325 210
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2270 2302 2535
Size: 2270 32 233
Fragments arranged by size:
2270 233 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1225 2535
Size: 1225 1310
ApaI G_GGCC'C
Cuts at: 0 1975 2535
Size: 1975 560
ApoI r'AATT_y
Cuts at: 0 574 2535
Size: 574 1961
AvaI C'yCGr_G
Cuts at: 0 34 2528 2535
Size: 34 2494 7
Fragments arranged by size:
2494 34 7
BamHI G'GATC_C
Cuts at: 0 1298 2535
Size: 1298 1237
BanI G'GyrC_C
Cuts at: 0 9 2535
Size: 9 2526
BanII G_rGCy'C
Cuts at: 0 35 67 290 1059 1409 1433 1802 1975
Size: 35 32 223 769 350 24 369 173
Cuts at: 1975 2535
Size: 560
Fragments arranged by size:
769 560 369 350 223 173 35 32
24
BbeI G_GCGC'C
Cuts at: 0 13 2535
Size: 13 2522
BbsI GAAGACnn'nnnn_
Cuts at: 0 1501 2480 2535
Size: 1501 979 55
Fragments arranged by size:
1501 979 55
BfrBI ATG'CAT
Cuts at: 0 901 2535
Size: 901 1634
BglI GCCn_nnn'nGGC
Cuts at: 0 667 1267 2186 2535
Size: 667 600 919 349
Fragments arranged by size:
919 667 600 349
BglII A'GATC_T
Cuts at: 0 2216 2535
Size: 2216 319
BlpI GC'TnA_GC
Cuts at: 0 560 2261 2535
Size: 560 1701 274
Fragments arranged by size:
1701 560 274
Bme1580I G_kGCm'C
Cuts at: 0 1395 1975 2535
Size: 1395 580 560
Fragments arranged by size:
1395 580 560
BmrI ACTGGGnnnn_n'
Cuts at: 0 1274 2535
Size: 1274 1261
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 891 1085 2535
Size: 891 194 1450
Fragments arranged by size:
1450 891 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1465 1850 2212 2535
Size: 937 528 385 362 323
Fragments arranged by size:
937 528 385 362 323
Bpu10I CC'TnA_GC
Cuts at: 0 452 1060 1796 2535
Size: 452 608 736 739
Fragments arranged by size:
739 736 608 452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 502 1021 2535
Size: 160 342 519 1514
Fragments arranged by size:
1514 519 342 160
BsaHI Gr'CG_yC
Cuts at: 0 10 2116 2535
Size: 10 2106 419
Fragments arranged by size:
2106 419 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 2535
Size: 22 30 2483
Fragments arranged by size:
2483 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 442 445 613 724 727 737 1024
Size: 346 96 3 168 111 3 10 287
Cuts at: 1024 1192 2125 2452 2535
Size: 168 933 327 83
Fragments arranged by size:
933 346 327 287 168 168 111 96
83 10 3 3
BseYI C'CCAG_C
Cuts at: 0 154 1568 1872 2535
Size: 154 1414 304 663
Fragments arranged by size:
1414 663 304 154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1527 2535
Size: 497 1030 1008
Fragments arranged by size:
1030 1008 497
BsiEI CG_ry'CG
Cuts at: 0 987 2535
Size: 987 1548
BsiHKAI G_wGCw'C
Cuts at: 0 61 176 290 1802 2535
Size: 61 115 114 1512 733
Fragments arranged by size:
1512 733 115 114 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1515 2107 2535
Size: 1515 592 428
Fragments arranged by size:
1515 592 428
Bsp1286I G_dGCh'C
Cuts at: 0 35 61 67 176 290 1059 1395 1409
Size: 35 26 6 109 114 769 336 14
Cuts at: 1409 1433 1802 1975 2535
Size: 24 369 173 560
Fragments arranged by size:
769 560 369 336 173 114 109 35
26 24 14 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2138 2156 2535
Size: 2138 18 379
Fragments arranged by size:
2138 379 18
BsrBI CCG'CTC
Cuts at: 0 937 1182 2535
Size: 937 245 1353
Fragments arranged by size:
1353 937 245
BsrDI GCAATG_nn'
Cuts at: 0 1043 2535
Size: 1043 1492
BsrFI r'CCGG_y
Cuts at: 0 982 2057 2522 2535
Size: 982 1075 465 13
Fragments arranged by size:
1075 982 465 13
BsrGI T'GTAC_A
Cuts at: 0 971 2535
Size: 971 1564
BssSI C'ACGA_G
Cuts at: 0 60 2535
Size: 60 2475
BstAPI GCAn_nnn'nTGC
Cuts at: 0 832 2535
Size: 832 1703
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1443 2316 2461 2535
Size: 1443 873 145 74
Fragments arranged by size:
1443 873 145 74
BstYI r'GATC_y
Cuts at: 0 1298 2216 2535
Size: 1298 918 319
Fragments arranged by size:
1298 918 319
Bsu36I CC'TnA_GG
Cuts at: 0 497 1814 2535
Size: 497 1317 721
Fragments arranged by size:
1317 721 497
BtgI C'CryG_G
Cuts at: 0 360 2034 2535
Size: 360 1674 501
Fragments arranged by size:
1674 501 360
BtsI GCAGTG_nn'
Cuts at: 0 1411 2535
Size: 1411 1124
ClaI AT'CG_AT
Cuts at: 0 2459 2535
Size: 2459 76
EaeI y'GGCC_r
Cuts at: 0 984 1385 2535
Size: 984 401 1150
Fragments arranged by size:
1150 984 401
EagI C'GGCC_G
Cuts at: 0 984 2535
Size: 984 1551
EarI CTCTTCn'nnn_
Cuts at: 0 122 158 272 419 533 590 701 1169
Size: 122 36 114 147 114 57 111 468
Cuts at: 1169 1984 2535
Size: 815 551
Fragments arranged by size:
815 551 468 147 122 114 114 111
57 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 264 880 1311 1506 1726 2535
Size: 264 616 431 195 220 809
Fragments arranged by size:
809 616 431 264 220 195
EcoICRI GAG'CTC
Cuts at: 0 288 1800 2535
Size: 288 1512 735
Fragments arranged by size:
1512 735 288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1174 1465 1693 1702 1825 1850 2212
Size: 937 237 291 228 9 123 25 362
Cuts at: 2212 2535
Size: 323
Fragments arranged by size:
937 362 323 291 237 228 123 25
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 406 2535
Size: 406 2129
EcoO109I rG'GnC_Cy
Cuts at: 0 1006 1791 1972 2499 2535
Size: 1006 785 181 527 36
Fragments arranged by size:
1006 785 527 181 36
EcoRV GAT'ATC
Cuts at: 0 1551 2535
Size: 1551 984
FspI TGC'GCA
Cuts at: 0 2104 2535
Size: 2104 431
HaeII r_GCGC'y
Cuts at: 0 13 807 1812 2535
Size: 13 794 1005 723
Fragments arranged by size:
1005 794 723 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 396 428 1765 1797 2311 2343 2535
Size: 396 32 1337 32 514 32 192
Fragments arranged by size:
1337 514 396 192 32 32 32
KasI G'GCGC_C
Cuts at: 0 9 2535
Size: 9 2526
MfeI C'AATT_G
Cuts at: 0 2415 2535
Size: 2415 120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2474 2535
Size: 2474 61
MscI TGG'CCA
Cuts at: 0 1387 2535
Size: 1387 1148
MslI CAynn'nnrTG
Cuts at: 0 1081 1562 1945 2535
Size: 1081 481 383 590
Fragments arranged by size:
1081 590 481 383
MspA1I CmG'CkG
Cuts at: 0 362 838 1200 1222 1276 1572 2100 2136
Size: 362 476 362 22 54 296 528 36
Cuts at: 2136 2424 2535
Size: 288 111
Fragments arranged by size:
528 476 362 362 296 288 111 54
36 22
NaeI GCC'GGC
Cuts at: 0 984 2524 2535
Size: 984 1540 11
Fragments arranged by size:
1540 984 11
NarI GG'CG_CC
Cuts at: 0 10 2535
Size: 10 2525
NcoI C'CATG_G
Cuts at: 0 2034 2535
Size: 2034 501
NgoMIV G'CCGG_C
Cuts at: 0 982 2522 2535
Size: 982 1540 13
Fragments arranged by size:
1540 982 13
NsiI A_TGCA'T
Cuts at: 0 903 2535
Size: 903 1632
NspI r_CATG'y
Cuts at: 0 1374 2535
Size: 1374 1161
PasI CC'CwG_GG
Cuts at: 0 2502 2535
Size: 2502 33
PpuMI rG'GwC_Cy
Cuts at: 0 1006 2535
Size: 1006 1529
PsiI TTA'TAA
Cuts at: 0 622 2535
Size: 622 1913
PspOMI G'GGCC_C
Cuts at: 0 1971 2535
Size: 1971 564
PstI C_TGCA'G
Cuts at: 0 1281 2135 2535
Size: 1281 854 400
Fragments arranged by size:
1281 854 400
PvuII CAG'CTG
Cuts at: 0 838 1200 1222 1276 2100 2424 2535
Size: 838 362 22 54 824 324 111
Fragments arranged by size:
838 824 362 324 111 54 22
SacI G_AGCT'C
Cuts at: 0 290 1802 2535
Size: 290 1512 733
Fragments arranged by size:
1512 733 290
SacII CC_GC'GG
Cuts at: 0 363 2535
Size: 363 2172
SapI GCTCTTCn'nnn_
Cuts at: 0 122 2535
Size: 122 2413
SfcI C'TryA_G
Cuts at: 0 1277 1584 2131 2535
Size: 1277 307 547 404
Fragments arranged by size:
1277 547 404 307
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2186 2535
Size: 2186 349
SfoI GGC'GCC
Cuts at: 0 11 2535
Size: 11 2524
SmlI C'TyrA_G
Cuts at: 0 175 301 517 1036 2535
Size: 175 126 216 519 1499
Fragments arranged by size:
1499 519 216 175 126
SphI G_CATG'C
Cuts at: 0 1374 2535
Size: 1374 1161
StyI C'CwwG_G
Cuts at: 0 377 2034 2535
Size: 377 1657 501
Fragments arranged by size:
1657 501 377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2418 2494 2535
Size: 2418 76 41
Fragments arranged by size:
2418 76 41
TatI w'GTAC_w
Cuts at: 0 971 1820 2535
Size: 971 849 715
Fragments arranged by size:
971 849 715
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 22 54 1112 1144 1283 1315 2535
Size: 22 32 1058 32 139 32 1220
Fragments arranged by size:
1220 1058 139 32 32 32 22
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1440 2535
Size: 1440 1095
Enzymes that do cut:
AclI AcuI AflII AhdI AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtsI ClaI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI
HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PpuMI
PsiI PspOMI PstI PvuII SacI SacII SapI SfcI
SfiI SfoI SmlI SphI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflIII AgeI AleI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI
BcgI BciVI BclI BmgBI BmtI BsaI BsaAI BsaBI
BsaWI BsiWI BsmI BspEI BspHI BssHII BstBI BstEII
BstZ17I BtgZI CspCI DraI DraIII DrdI EcoRI FalI
FseI FspAI HincII HindIII HpaI KpnI MluI NdeI
NheI NotI NruI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PspXI PsrI PvuI RsrII SalI
SanDI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI