Restriction map for
pFN21AE5705
(Linear) (Six-base) MAPSORT of: KIEE5705.seq Check: 5924 from: 1 to: 1869
KIEE5705.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 758 1869
Size: 758 1111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 310 356 1869
Size: 2 308 46 1513
Fragments arranged by size:
1513 308 46 2
AhdI GACnn_n'nnGTC
Cuts at: 0 470 1869
Size: 470 1399
AleI CACnn'nnGTG
Cuts at: 0 1276 1869
Size: 1276 593
AlwNI CAG_nnn'CTG
Cuts at: 0 266 1087 1198 1869
Size: 266 821 111 671
Fragments arranged by size:
821 671 266 111
ApoI r'AATT_y
Cuts at: 0 966 1598 1869
Size: 966 632 271
Fragments arranged by size:
966 632 271
AvaI C'yCGr_G
Cuts at: 0 1245 1570 1825 1869
Size: 1245 325 255 44
Fragments arranged by size:
1245 325 255 44
BamHI G'GATC_C
Cuts at: 0 340 785 1869
Size: 340 445 1084
Fragments arranged by size:
1084 445 340
BanI G'GyrC_C
Cuts at: 0 1171 1267 1869
Size: 1171 96 602
Fragments arranged by size:
1171 602 96
BanII G_rGCy'C
Cuts at: 0 824 999 1538 1571 1869
Size: 824 175 539 33 298
Fragments arranged by size:
824 539 298 175 33
BbeI G_GCGC'C
Cuts at: 0 1175 1869
Size: 1175 694
BbsI GAAGACnn'nnnn_
Cuts at: 0 317 1642 1869
Size: 317 1325 227
Fragments arranged by size:
1325 317 227
BbvCI CC'TCA_GC
Cuts at: 0 1097 1869
Size: 1097 772
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 157 191 1869
Size: 157 34 1678
Fragments arranged by size:
1678 157 34
BclI T'GATC_A
Cuts at: 0 365 1869
Size: 365 1504
BfrBI ATG'CAT
Cuts at: 0 1293 1869
Size: 1293 576
BglII A'GATC_T
Cuts at: 0 1076 1869
Size: 1076 793
Bme1580I G_kGCm'C
Cuts at: 0 184 1869
Size: 184 1685
BmrI ACTGGGnnnn_n'
Cuts at: 0 514 728 1869
Size: 514 214 1141
Fragments arranged by size:
1141 514 214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1013 1869
Size: 1013 856
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1063 1869
Size: 223 840 806
Fragments arranged by size:
840 806 223
Bpu10I CC'TnA_GC
Cuts at: 0 476 814 1097 1135 1869
Size: 476 338 283 38 734
Fragments arranged by size:
734 476 338 283 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1327 1869
Size: 808 519 542
Fragments arranged by size:
808 542 519
BsaI GGTCTCn'nnnn_
Cuts at: 0 892 1869
Size: 892 977
BsaBI GATnn'nnATC
Cuts at: 0 110 1869
Size: 110 1759
BsaHI Gr'CG_yC
Cuts at: 0 728 1172 1869
Size: 728 444 697
Fragments arranged by size:
728 697 444
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 847 877 1869
Size: 847 30 992
Fragments arranged by size:
992 847 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 487 1114 1869
Size: 487 627 755
Fragments arranged by size:
755 627 487
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1385 1869
Size: 1385 484
BsiHKAI G_wGCw'C
Cuts at: 0 653 749 824 1254 1869
Size: 653 96 75 430 615
Fragments arranged by size:
653 615 430 96 75
BsmI GAATG_Cn'
Cuts at: 0 1330 1869
Size: 1330 539
BsmBI CGTCTCn'nnnn_
Cuts at: 0 685 1195 1837 1869
Size: 685 510 642 32
Fragments arranged by size:
685 642 510 32
Bsp1286I G_dGCh'C
Cuts at: 0 184 653 749 824 999 1254 1538 1571
Size: 184 469 96 75 175 255 284 33
Cuts at: 1571 1869
Size: 298
Fragments arranged by size:
469 298 284 255 184 175 96 75
33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 758 1780 1869
Size: 758 1022 89
Fragments arranged by size:
1022 758 89
BsrFI r'CCGG_y
Cuts at: 0 415 1869
Size: 415 1454
BstEII G'GTnAC_C
Cuts at: 0 715 1393 1869
Size: 715 678 476
Fragments arranged by size:
715 678 476
BstXI CCAn_nnnn'nTGG
Cuts at: 0 255 1869
Size: 255 1614
BstYI r'GATC_y
Cuts at: 0 340 785 1076 1869
Size: 340 445 291 793
Fragments arranged by size:
793 445 340 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 933 1869
Size: 933 936
BtsI GCAGTG_nn'
Cuts at: 0 596 1869
Size: 596 1273
EaeI y'GGCC_r
Cuts at: 0 395 417 1869
Size: 395 22 1452
Fragments arranged by size:
1452 395 22
EarI CTCTTCn'nnn_
Cuts at: 0 194 709 1869
Size: 194 515 1160
Fragments arranged by size:
1160 515 194
EcoICRI GAG'CTC
Cuts at: 0 822 1869
Size: 822 1047
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 223 310 356 1063 1869
Size: 2 221 87 46 707 806
Fragments arranged by size:
806 707 221 87 46 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1225 1869
Size: 1225 644
EcoO109I rG'GnC_Cy
Cuts at: 0 828 1162 1211 1301 1498 1869
Size: 828 334 49 90 197 371
Fragments arranged by size:
828 371 334 197 90 49
EcoRI G'AATT_C
Cuts at: 0 966 1598 1869
Size: 966 632 271
Fragments arranged by size:
966 632 271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1344 1414 1869
Size: 1344 70 455
Fragments arranged by size:
1344 455 70
HaeII r_GCGC'y
Cuts at: 0 231 483 1175 1869
Size: 231 252 692 694
Fragments arranged by size:
694 692 252 231
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 456 488 1869
Size: 456 32 1381
Fragments arranged by size:
1381 456 32
HincII GTy'rAC
Cuts at: 0 453 1869
Size: 453 1416
KasI G'GCGC_C
Cuts at: 0 1171 1869
Size: 1171 698
MscI TGG'CCA
Cuts at: 0 397 1869
Size: 397 1472
MslI CAynn'nnrTG
Cuts at: 0 116 1276 1357 1869
Size: 116 1160 81 512
Fragments arranged by size:
1160 512 116 81
MspA1I CmG'CkG
Cuts at: 0 36 920 973 1084 1869
Size: 36 884 53 111 785
Fragments arranged by size:
884 785 111 53 36
NaeI GCC'GGC
Cuts at: 0 417 1869
Size: 417 1452
NarI GG'CG_CC
Cuts at: 0 1172 1869
Size: 1172 697
NdeI CA'TA_TG
Cuts at: 0 1335 1869
Size: 1335 534
NgoMIV G'CCGG_C
Cuts at: 0 415 1869
Size: 415 1454
NsiI A_TGCA'T
Cuts at: 0 1295 1869
Size: 1295 574
NspI r_CATG'y
Cuts at: 0 208 1869
Size: 208 1661
PfoI T'CCnGG_A
Cuts at: 0 529 1869
Size: 529 1340
PpuMI rG'GwC_Cy
Cuts at: 0 1162 1211 1301 1498 1869
Size: 1162 49 90 197 371
Fragments arranged by size:
1162 371 197 90 49
PshAI GACnn'nnGTC
Cuts at: 0 764 1869
Size: 764 1105
PstI C_TGCA'G
Cuts at: 0 1777 1869
Size: 1777 92
PvuII CAG'CTG
Cuts at: 0 36 973 1084 1869
Size: 36 937 111 785
Fragments arranged by size:
937 785 111 36
SacI G_AGCT'C
Cuts at: 0 824 1869
Size: 824 1045
SanDI GG'GwC_CC
Cuts at: 0 1162 1301 1869
Size: 1162 139 568
Fragments arranged by size:
1162 568 139
SapI GCTCTTCn'nnn_
Cuts at: 0 194 709 1869
Size: 194 515 1160
Fragments arranged by size:
1160 515 194
SbfI CC_TGCA'GG
Cuts at: 0 1777 1869
Size: 1777 92
SfcI C'TryA_G
Cuts at: 0 374 1307 1773 1869
Size: 374 933 466 96
Fragments arranged by size:
933 466 374 96
SfoI GGC'GCC
Cuts at: 0 1173 1869
Size: 1173 696
SmaI CCC'GGG
Cuts at: 0 1572 1869
Size: 1572 297
SmlI C'TyrA_G
Cuts at: 0 823 1342 1825 1869
Size: 823 519 483 44
Fragments arranged by size:
823 519 483 44
SphI G_CATG'C
Cuts at: 0 208 1869
Size: 208 1661
StuI AGG'CCT
Cuts at: 0 85 1439 1869
Size: 85 1354 430
Fragments arranged by size:
1354 430 85
StyI C'CwwG_G
Cuts at: 0 602 850 908 1538 1869
Size: 602 248 58 630 331
Fragments arranged by size:
630 602 331 248 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 1869
Size: 85 1784
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 629 661 847 879 1869
Size: 629 32 186 32 990
Fragments arranged by size:
990 629 186 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1215 1869
Size: 1215 654
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 351 1869
Size: 351 1518
XhoI C'TCGA_G
Cuts at: 0 1825 1869
Size: 1825 44
XmaI C'CCGG_G
Cuts at: 0 1570 1869
Size: 1570 299
Enzymes that do cut:
AarI AcuI AhdI AleI AlwNI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI
BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrFI BstEII BstXI BstYI BtgZI BtsI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NsiI NspI PfoI PpuMI PshAI
PstI PvuII SacI SanDI SapI SbfI SfcI SfoI
SmaI SmlI SphI StuI StyI TaqII TstI Tth111I
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BciVI BglI BlpI BmgBI BmtI BsaAI BsaWI
BseYI BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoRV FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI
NcoI NheI NotI NruI PacI PasI PciI PflMI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TatI XbaI XmnI
ZraI