Restriction map for
pFN21AE5701
(Linear) (Six-base) MAPSORT of: KIEE5701.seq Check: 535 from: 1 to: 2244
KIEE5701.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AccI GT'mk_AC
Cuts at: 0 803 1925 2244
Size: 803 1122 319
Fragments arranged by size:
1122 803 319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 418 1684 1747 2191 2244
Size: 380 38 1266 63 444 53
Fragments arranged by size:
1266 444 380 63 53 38
AfeI AGC'GCT
Cuts at: 0 943 1355 2244
Size: 943 412 889
Fragments arranged by size:
943 889 412
AleI CACnn'nnGTG
Cuts at: 0 1091 1912 2244
Size: 1091 821 332
Fragments arranged by size:
1091 821 332
AlwNI CAG_nnn'CTG
Cuts at: 0 1626 2244
Size: 1626 618
ApaI G_GGCC'C
Cuts at: 0 318 2244
Size: 318 1926
ApoI r'AATT_y
Cuts at: 0 256 1777 2244
Size: 256 1521 467
Fragments arranged by size:
1521 467 256
AvaI C'yCGr_G
Cuts at: 0 716 1738 1805 1867 2244
Size: 716 1022 67 62 377
Fragments arranged by size:
1022 716 377 67 62
AvrII C'CTAG_G
Cuts at: 0 1065 2244
Size: 1065 1179
BamHI G'GATC_C
Cuts at: 0 432 2244
Size: 432 1812
BanI G'GyrC_C
Cuts at: 0 858 1057 2244
Size: 858 199 1187
Fragments arranged by size:
1187 858 199
BanII G_rGCy'C
Cuts at: 0 318 2147 2244
Size: 318 1829 97
Fragments arranged by size:
1829 318 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 1498 1895 2244
Size: 1498 397 349
Fragments arranged by size:
1498 397 349
BglII A'GATC_T
Cuts at: 0 1667 2244
Size: 1667 577
BlpI GC'TnA_GC
Cuts at: 0 1837 2146 2244
Size: 1837 309 98
Fragments arranged by size:
1837 309 98
Bme1580I G_kGCm'C
Cuts at: 0 200 318 1006 2244
Size: 200 118 688 1238
Fragments arranged by size:
1238 688 200 118
BmgBI CAC'GTC
Cuts at: 0 152 2244
Size: 152 2092
BmrI ACTGGGnnnn_n'
Cuts at: 0 42 2054 2244
Size: 42 2012 190
Fragments arranged by size:
2012 190 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 858 960 1710 2244
Size: 858 102 750 534
Fragments arranged by size:
858 750 534 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 1317 1651 2244
Size: 686 631 334 593
Fragments arranged by size:
686 631 593 334
Bpu10I CC'TnA_GC
Cuts at: 0 910 2189 2244
Size: 910 1279 55
Fragments arranged by size:
1279 910 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 249 517 2244
Size: 116 133 268 1727
Fragments arranged by size:
1727 268 133 116
BsaI GGTCTCn'nnnn_
Cuts at: 0 418 2244
Size: 418 1826
BsaWI w'CCGG_w
Cuts at: 0 1106 2244
Size: 1106 1138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 518 548 2077 2107 2244
Size: 518 30 1529 30 137
Fragments arranged by size:
1529 518 137 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1990 2011 2244
Size: 1990 21 233
Fragments arranged by size:
1990 233 21
BseYI C'CCAG_C
Cuts at: 0 64 71 1166 2244
Size: 64 7 1095 1078
Fragments arranged by size:
1095 1078 64 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 496 529 1168 2244
Size: 61 435 33 639 1076
Fragments arranged by size:
1076 639 435 61 33
BsiEI CG_ry'CG
Cuts at: 0 1712 2244
Size: 1712 532
BsiHKAI G_wGCw'C
Cuts at: 0 1045 2147 2244
Size: 1045 1102 97
Fragments arranged by size:
1102 1045 97
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1956 2244
Size: 1956 288
Bsp1286I G_dGCh'C
Cuts at: 0 200 318 1006 1045 2147 2244
Size: 200 118 688 39 1102 97
Fragments arranged by size:
1102 688 200 118 97 39
BsrDI GCAATG_nn'
Cuts at: 0 1202 2244
Size: 1202 1042
BsrFI r'CCGG_y
Cuts at: 0 32 2244
Size: 32 2212
BssSI C'ACGA_G
Cuts at: 0 1308 2244
Size: 1308 936
BstAPI GCAn_nnn'nTGC
Cuts at: 0 474 2244
Size: 474 1770
BstEII G'GTnAC_C
Cuts at: 0 904 2244
Size: 904 1340
BstYI r'GATC_y
Cuts at: 0 432 1667 2244
Size: 432 1235 577
Fragments arranged by size:
1235 577 432
Bsu36I CC'TnA_GG
Cuts at: 0 863 2244
Size: 863 1381
BtgI C'CryG_G
Cuts at: 0 428 1049 1343 2244
Size: 428 621 294 901
Fragments arranged by size:
901 621 428 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1623 1856 2244
Size: 1623 233 388
Fragments arranged by size:
1623 388 233
BtsI GCAGTG_nn'
Cuts at: 0 479 2244
Size: 479 1765
DraIII CAC_nnn'GTG
Cuts at: 0 474 2244
Size: 474 1770
DrdI GACnn_nn'nnGTC
Cuts at: 0 1168 2244
Size: 1168 1076
EaeI y'GGCC_r
Cuts at: 0 997 1046 2039 2244
Size: 997 49 993 205
Fragments arranged by size:
997 993 205 49
EarI CTCTTCn'nnn_
Cuts at: 0 228 346 1541 1603 1679 1967 1975 2185
Size: 228 118 1195 62 76 288 8 210
Cuts at: 2185 2244
Size: 59
Fragments arranged by size:
1195 288 228 210 118 76 62 59
8
EcoICRI GAG'CTC
Cuts at: 0 2145 2244
Size: 2145 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 418 686 1317 1651 1684 1747 2191
Size: 380 38 268 631 334 33 63 444
Cuts at: 2191 2244
Size: 53
Fragments arranged by size:
631 444 380 334 268 63 53 38
33
EcoO109I rG'GnC_Cy
Cuts at: 0 68 206 314 1717 2244
Size: 68 138 108 1403 527
Fragments arranged by size:
1403 527 138 108 68
EcoRI G'AATT_C
Cuts at: 0 1777 2244
Size: 1777 467
EcoRV GAT'ATC
Cuts at: 0 225 1404 2244
Size: 225 1179 840
Fragments arranged by size:
1179 840 225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 267 1423 2196 2244
Size: 267 1156 773 48
Fragments arranged by size:
1156 773 267 48
HaeII r_GCGC'y
Cuts at: 0 945 1357 2244
Size: 945 412 887
Fragments arranged by size:
945 887 412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 417 449 518 550 565 597 1694 1726
Size: 417 32 69 32 15 32 1097 32
Cuts at: 1726 2077 2109 2244
Size: 351 32 135
Fragments arranged by size:
1097 417 351 135 69 32 32 32
32 32 15
HincII GTy'rAC
Cuts at: 0 537 1422 1527 2244
Size: 537 885 105 717
Fragments arranged by size:
885 717 537 105
HindIII A'AGCT_T
Cuts at: 0 2235 2244
Size: 2235 9
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 852 1121 1692 2244
Size: 852 269 571 552
Fragments arranged by size:
852 571 552 269
MscI TGG'CCA
Cuts at: 0 2041 2244
Size: 2041 203
MslI CAynn'nnrTG
Cuts at: 0 845 1091 1124 1540 1657 1750 1912 2244
Size: 845 246 33 416 117 93 162 332
Fragments arranged by size:
845 416 332 246 162 117 93 33
MspA1I CmG'CkG
Cuts at: 0 542 2244
Size: 542 1702
NaeI GCC'GGC
Cuts at: 0 34 2244
Size: 34 2210
NcoI C'CATG_G
Cuts at: 0 1049 1343 2244
Size: 1049 294 901
Fragments arranged by size:
1049 901 294
NdeI CA'TA_TG
Cuts at: 0 1658 2244
Size: 1658 586
NgoMIV G'CCGG_C
Cuts at: 0 32 2244
Size: 32 2212
PasI CC'CwG_GG
Cuts at: 0 1698 2244
Size: 1698 546
PflMI CCAn_nnn'nTGG
Cuts at: 0 653 2244
Size: 653 1591
PpuMI rG'GwC_Cy
Cuts at: 0 68 206 2244
Size: 68 138 2038
Fragments arranged by size:
2038 138 68
PspOMI G'GGCC_C
Cuts at: 0 314 2244
Size: 314 1930
PstI C_TGCA'G
Cuts at: 0 924 1835 2219 2244
Size: 924 911 384 25
Fragments arranged by size:
924 911 384 25
PvuII CAG'CTG
Cuts at: 0 542 2244
Size: 542 1702
SacI G_AGCT'C
Cuts at: 0 2147 2244
Size: 2147 97
SanDI GG'GwC_CC
Cuts at: 0 68 2244
Size: 68 2176
SapI GCTCTTCn'nnn_
Cuts at: 0 2185 2244
Size: 2185 59
SfcI C'TryA_G
Cuts at: 0 920 1831 2215 2244
Size: 920 911 384 29
Fragments arranged by size:
920 911 384 29
SmaI CCC'GGG
Cuts at: 0 718 2244
Size: 718 1526
SmlI C'TyrA_G
Cuts at: 0 131 228 496 1738 2244
Size: 131 97 268 1242 506
Fragments arranged by size:
1242 506 268 131 97
StuI AGG'CCT
Cuts at: 0 357 973 1147 2244
Size: 357 616 174 1097
Fragments arranged by size:
1097 616 357 174
StyI C'CwwG_G
Cuts at: 0 569 1006 1049 1065 1343 1559 2244
Size: 569 437 43 16 278 216 685
Fragments arranged by size:
685 569 437 278 216 43 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1669 2244
Size: 1669 575
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 190 222 2244
Size: 190 32 2022
Fragments arranged by size:
2022 190 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1341 2127 2244
Size: 1341 786 117
Fragments arranged by size:
1341 786 117
XhoI C'TCGA_G
Cuts at: 0 1738 2244
Size: 1738 506
XmaI C'CCGG_G
Cuts at: 0 716 2244
Size: 716 1528
XmnI GAAnn'nnTTC
Cuts at: 0 461 2179 2244
Size: 461 1718 65
Fragments arranged by size:
1718 461 65
Enzymes that do cut:
AccI AcuI AfeI AleI AlwNI ApaI ApoI AvaI
AvrII BamHI BanI BanII BbsI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BsrDI BsrFI BssSI BstAPI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
PasI PflMI PpuMI PspOMI PstI PvuII SacI SanDI
SapI SfcI SmaI SmlI StuI StyI TaqII TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BmtI BsaAI
BsaBI BsaHI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrGI BssHII BstBI BstXI BstZ17I ClaI CspCI DraI
EagI EciI EcoNI FseI FspI FspAI HpaI KasI
KpnI MfeI MluI NarI NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI ZraI