Restriction map for
pFN21AE5694
(Linear) (Six-base) MAPSORT of: KIEE5694.seq Check: 4529 from: 1 to: 1620
KIEE5694.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AccI GT'mk_AC
Cuts at: 0 297 816 1620
Size: 297 519 804
Fragments arranged by size:
804 519 297
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 746 1135 1620
Size: 458 288 389 485
Fragments arranged by size:
485 458 389 288
AleI CACnn'nnGTG
Cuts at: 0 457 1393 1620
Size: 457 936 227
Fragments arranged by size:
936 457 227
AlwNI CAG_nnn'CTG
Cuts at: 0 1020 1045 1195 1620
Size: 1020 25 150 425
Fragments arranged by size:
1020 425 150 25
ApaLI G'TGCA_C
Cuts at: 0 1144 1620
Size: 1144 476
AscI GG'CGCG_CC
Cuts at: 0 238 1620
Size: 238 1382
AvaI C'yCGr_G
Cuts at: 0 168 611 635 1340 1373 1620
Size: 168 443 24 705 33 247
Fragments arranged by size:
705 443 247 168 33 24
BanI G'GyrC_C
Cuts at: 0 54 345 1482 1489 1620
Size: 54 291 1137 7 131
Fragments arranged by size:
1137 291 131 54 7
BanII G_rGCy'C
Cuts at: 0 404 795 1339 1620
Size: 404 391 544 281
Fragments arranged by size:
544 404 391 281
BbeI G_GCGC'C
Cuts at: 0 58 349 1620
Size: 58 291 1271
Fragments arranged by size:
1271 291 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 956 1564 1620
Size: 956 608 56
Fragments arranged by size:
956 608 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 159 193 1620
Size: 159 34 1427
Fragments arranged by size:
1427 159 34
BclI T'GATC_A
Cuts at: 0 821 1620
Size: 821 799
BglI GCCn_nnn'nGGC
Cuts at: 0 21 1620
Size: 21 1599
Bme1580I G_kGCm'C
Cuts at: 0 1148 1620
Size: 1148 472
BmrI ACTGGGnnnn_n'
Cuts at: 0 109 259 1620
Size: 109 150 1361
Fragments arranged by size:
1361 150 109
BmtI G_CTAG'C
Cuts at: 0 277 1620
Size: 277 1343
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 855 1620
Size: 855 765
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 730 770 850 1203 1620
Size: 418 312 40 80 353 417
Fragments arranged by size:
418 417 353 312 80 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 1138 1620
Size: 266 872 482
Fragments arranged by size:
872 482 266
BsaHI Gr'CG_yC
Cuts at: 0 55 346 1620
Size: 55 291 1274
Fragments arranged by size:
1274 291 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 304 334 1620
Size: 304 30 1286
Fragments arranged by size:
1286 304 30
BseYI C'CCAG_C
Cuts at: 0 936 1236 1620
Size: 936 300 384
Fragments arranged by size:
936 384 300
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 481 1341 1620
Size: 397 84 860 279
Fragments arranged by size:
860 397 279 84
BsiEI CG_ry'CG
Cuts at: 0 16 220 495 1620
Size: 16 204 275 1125
Fragments arranged by size:
1125 275 204 16
BsiHKAI G_wGCw'C
Cuts at: 0 1148 1339 1620
Size: 1148 191 281
Fragments arranged by size:
1148 281 191
Bsp1286I G_dGCh'C
Cuts at: 0 404 795 1148 1339 1620
Size: 404 391 353 191 281
Fragments arranged by size:
404 391 353 281 191
BsrBI CCG'CTC
Cuts at: 0 683 1620
Size: 683 937
BsrFI r'CCGG_y
Cuts at: 0 15 99 174 234 520 1620
Size: 15 84 75 60 286 1100
Fragments arranged by size:
1100 286 84 75 60 15
BssHII G'CGCG_C
Cuts at: 0 238 264 266 302 330 332 1620
Size: 238 26 2 36 28 2 1288
Fragments arranged by size:
1288 238 36 28 26 2 2
BssSI C'ACGA_G
Cuts at: 0 34 1620
Size: 34 1586
Bsu36I CC'TnA_GG
Cuts at: 0 1188 1273 1620
Size: 1188 85 347
Fragments arranged by size:
1188 347 85
BtgI C'CryG_G
Cuts at: 0 720 1620
Size: 720 900
BtsI GCAGTG_nn'
Cuts at: 0 531 868 1620
Size: 531 337 752
Fragments arranged by size:
752 531 337
DrdI GACnn_nn'nnGTC
Cuts at: 0 305 824 1620
Size: 305 519 796
Fragments arranged by size:
796 519 305
EaeI y'GGCC_r
Cuts at: 0 13 217 492 779 1133 1620
Size: 13 204 275 287 354 487
Fragments arranged by size:
487 354 287 275 204 13
EagI C'GGCC_G
Cuts at: 0 13 217 492 1620
Size: 13 204 275 1128
Fragments arranged by size:
1128 275 204 13
EarI CTCTTCn'nnn_
Cuts at: 0 286 910 1327 1620
Size: 286 624 417 293
Fragments arranged by size:
624 417 293 286
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 174 1620
Size: 174 1446
EcoICRI GAG'CTC
Cuts at: 0 1337 1620
Size: 1337 283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 458 730 746 770 850 1135 1203
Size: 418 40 272 16 24 80 285 68
Cuts at: 1203 1620
Size: 417
Fragments arranged by size:
418 417 285 272 80 68 40 24
16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1302 1620
Size: 1302 318
EcoO109I rG'GnC_Cy
Cuts at: 0 66 705 804 1377 1620
Size: 66 639 99 573 243
Fragments arranged by size:
639 573 243 99 66
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 396 1620
Size: 396 1224
FspI TGC'GCA
Cuts at: 0 109 1620
Size: 109 1511
FspAI rTGC'GCAy
Cuts at: 0 109 1620
Size: 109 1511
HaeII r_GCGC'y
Cuts at: 0 58 349 1620
Size: 58 291 1271
Fragments arranged by size:
1271 291 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 53 85 304 336 605 637 1354 1386
Size: 53 32 219 32 269 32 717 32
Cuts at: 1386 1620
Size: 234
Fragments arranged by size:
717 269 234 219 53 32 32 32
32
HincII GTy'rAC
Cuts at: 0 298 817 1620
Size: 298 519 803
Fragments arranged by size:
803 519 298
HindIII A'AGCT_T
Cuts at: 0 882 1620
Size: 882 738
KasI G'GCGC_C
Cuts at: 0 54 345 1620
Size: 54 291 1275
Fragments arranged by size:
1275 291 54
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 833 1290 1620
Size: 116 717 457 330
Fragments arranged by size:
717 457 330 116
MscI TGG'CCA
Cuts at: 0 781 1620
Size: 781 839
MslI CAynn'nnrTG
Cuts at: 0 115 457 1393 1620
Size: 115 342 936 227
Fragments arranged by size:
936 342 227 115
MspA1I CmG'CkG
Cuts at: 0 129 144 198 352 555 747 940 1620
Size: 129 15 54 154 203 192 193 680
Fragments arranged by size:
680 203 193 192 154 129 54 15
NaeI GCC'GGC
Cuts at: 0 236 1620
Size: 236 1384
NarI GG'CG_CC
Cuts at: 0 55 346 1620
Size: 55 291 1274
Fragments arranged by size:
1274 291 55
NgoMIV G'CCGG_C
Cuts at: 0 234 1620
Size: 234 1386
NheI G'CTAG_C
Cuts at: 0 273 1620
Size: 273 1347
NotI GC'GGCC_GC
Cuts at: 0 492 1620
Size: 492 1128
NspI r_CATG'y
Cuts at: 0 729 1620
Size: 729 891
PasI CC'CwG_GG
Cuts at: 0 1517 1620
Size: 1517 103
PflMI CCAn_nnn'nTGG
Cuts at: 0 675 1620
Size: 675 945
PfoI T'CCnGG_A
Cuts at: 0 887 1575 1620
Size: 887 688 45
Fragments arranged by size:
887 688 45
PpuMI rG'GwC_Cy
Cuts at: 0 705 1620
Size: 705 915
PstI C_TGCA'G
Cuts at: 0 746 1620
Size: 746 874
PvuII CAG'CTG
Cuts at: 0 747 940 1620
Size: 747 193 680
Fragments arranged by size:
747 680 193
RsrII CG'GwC_CG
Cuts at: 0 75 193 1620
Size: 75 118 1427
Fragments arranged by size:
1427 118 75
SacI G_AGCT'C
Cuts at: 0 1339 1620
Size: 1339 281
SalI G'TCGA_C
Cuts at: 0 296 815 1620
Size: 296 519 805
Fragments arranged by size:
805 519 296
SapI GCTCTTCn'nnn_
Cuts at: 0 910 1327 1620
Size: 910 417 293
Fragments arranged by size:
910 417 293
SfcI C'TryA_G
Cuts at: 0 124 421 742 1620
Size: 124 297 321 878
Fragments arranged by size:
878 321 297 124
SfoI GGC'GCC
Cuts at: 0 56 347 1620
Size: 56 291 1273
Fragments arranged by size:
1273 291 56
SmlI C'TyrA_G
Cuts at: 0 281 1153 1620
Size: 281 872 467
Fragments arranged by size:
872 467 281
SphI G_CATG'C
Cuts at: 0 729 1620
Size: 729 891
StuI AGG'CCT
Cuts at: 0 617 1620
Size: 617 1003
StyI C'CwwG_G
Cuts at: 0 674 686 1620
Size: 674 12 934
Fragments arranged by size:
934 674 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 794 1620
Size: 794 826
XmnI GAAnn'nnTTC
Cuts at: 0 443 1620
Size: 443 1177
Enzymes that do cut:
AccI AcuI AleI AlwNI ApaLI AscI AvaI BanI
BanII BbeI BbsI BcgI BclI BglI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaHI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssHII BssSI Bsu36I
BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NotI NspI PasI PflMI PfoI
PpuMI PstI PvuII RsrII SacI SalI SapI SfcI
SfoI SmlI SphI StuI StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApoI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BfrBI BglII BlpI BmgBI
Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrGI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII EcoRI EcoRV FseI HpaI KpnI MfeI MluI
NcoI NdeI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI SacII
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI ZraI