Restriction map for
pFN21AE5686
(Linear) (Six-base) MAPSORT of: KIEE5686.seq Check: 9245 from: 1 to: 1689
KIEE5686.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
Acc65I G'GTAC_C
Cuts at: 0 506 1689
Size: 506 1183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1162 1327 1689
Size: 1162 165 362
Fragments arranged by size:
1162 362 165
AflIII A'CryG_T
Cuts at: 0 302 1689
Size: 302 1387
AleI CACnn'nnGTG
Cuts at: 0 941 1689
Size: 941 748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1588 1689
Size: 1588 101
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1477 1509 1689
Size: 1477 32 180
Fragments arranged by size:
1477 180 32
AlwNI CAG_nnn'CTG
Cuts at: 0 177 1689
Size: 177 1512
AvaI C'yCGr_G
Cuts at: 0 4 323 1689
Size: 4 319 1366
Fragments arranged by size:
1366 319 4
BanI G'GyrC_C
Cuts at: 0 148 506 575 1689
Size: 148 358 69 1114
Fragments arranged by size:
1114 358 148 69
BanII G_rGCy'C
Cuts at: 0 901 1103 1381 1460 1689
Size: 901 202 278 79 229
Fragments arranged by size:
901 278 229 202 79
BbvCI CC'TCA_GC
Cuts at: 0 976 998 1689
Size: 976 22 691
Fragments arranged by size:
976 691 22
BciVI GTATCCnnnnn_n'
Cuts at: 0 559 1689
Size: 559 1130
BclI T'GATC_A
Cuts at: 0 360 1256 1526 1689
Size: 360 896 270 163
Fragments arranged by size:
896 360 270 163
BfrBI ATG'CAT
Cuts at: 0 1230 1689
Size: 1230 459
BglI GCCn_nnn'nGGC
Cuts at: 0 193 710 1385 1411 1689
Size: 193 517 675 26 278
Fragments arranged by size:
675 517 278 193 26
BmgBI CAC'GTC
Cuts at: 0 305 366 1223 1689
Size: 305 61 857 466
Fragments arranged by size:
857 466 305 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 671 1415 1689
Size: 671 744 274
Fragments arranged by size:
744 671 274
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 915 954 1689
Size: 915 39 735
Fragments arranged by size:
915 735 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 924 1600 1689
Size: 924 676 89
Fragments arranged by size:
924 676 89
Bpu10I CC'TnA_GC
Cuts at: 0 197 914 976 998 1689
Size: 197 717 62 22 691
Fragments arranged by size:
717 691 197 62 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 727 1689
Size: 148 579 962
Fragments arranged by size:
962 579 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 635 758 1123 1497 1689
Size: 635 123 365 374 192
Fragments arranged by size:
635 374 365 192 123
BsaBI GATnn'nnATC
Cuts at: 0 1669 1689
Size: 1669 20
BsaHI Gr'CG_yC
Cuts at: 0 590 613 1689
Size: 590 23 1076
Fragments arranged by size:
1076 590 23
BsaWI w'CCGG_w
Cuts at: 0 644 1082 1689
Size: 644 438 607
Fragments arranged by size:
644 607 438
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 490 520 1477 1507 1689
Size: 490 30 957 30 182
Fragments arranged by size:
957 490 182 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 511 1258 1689
Size: 124 387 747 431
Fragments arranged by size:
747 431 387 124
BseYI C'CCAG_C
Cuts at: 0 512 1176 1301 1316 1604 1689
Size: 512 664 125 15 288 85
Fragments arranged by size:
664 512 288 125 85 15
BsiEI CG_ry'CG
Cuts at: 0 906 1689
Size: 906 783
BsmI GAATG_Cn'
Cuts at: 0 347 1689
Size: 347 1342
BsmBI CGTCTCn'nnnn_
Cuts at: 0 280 1553 1689
Size: 280 1273 136
Fragments arranged by size:
1273 280 136
Bsp1286I G_dGCh'C
Cuts at: 0 901 1103 1381 1460 1689
Size: 901 202 278 79 229
Fragments arranged by size:
901 278 229 202 79
BsrBI CCG'CTC
Cuts at: 0 894 1689
Size: 894 795
BssSI C'ACGA_G
Cuts at: 0 1027 1126 1689
Size: 1027 99 563
Fragments arranged by size:
1027 563 99
BstEII G'GTnAC_C
Cuts at: 0 43 1689
Size: 43 1646
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1110 1285 1689
Size: 1110 175 404
Fragments arranged by size:
1110 404 175
BtgI C'CryG_G
Cuts at: 0 135 768 825 1047 1689
Size: 135 633 57 222 642
Fragments arranged by size:
642 633 222 135 57
BtsI GCAGTG_nn'
Cuts at: 0 882 1682 1689
Size: 882 800 7
Fragments arranged by size:
882 800 7
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 858 893 1689
Size: 858 35 796
Fragments arranged by size:
858 796 35
DraIII CAC_nnn'GTG
Cuts at: 0 1662 1689
Size: 1662 27
DrdI GACnn_nn'nnGTC
Cuts at: 0 957 1689
Size: 957 732
EaeI y'GGCC_r
Cuts at: 0 138 203 1689
Size: 138 65 1486
Fragments arranged by size:
1486 138 65
EarI CTCTTCn'nnn_
Cuts at: 0 40 785 1047 1109 1689
Size: 40 745 262 62 580
Fragments arranged by size:
745 580 262 62 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 924 1162 1327 1600 1689
Size: 924 238 165 273 89
Fragments arranged by size:
924 273 238 165 89
EcoO109I rG'GnC_Cy
Cuts at: 0 83 295 1689
Size: 83 212 1394
Fragments arranged by size:
1394 212 83
FspI TGC'GCA
Cuts at: 0 730 1689
Size: 730 959
HaeII r_GCGC'y
Cuts at: 0 1323 1689
Size: 1323 366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 345 377 1248 1280 1689
Size: 345 32 871 32 409
Fragments arranged by size:
871 409 345 32 32
HindIII A'AGCT_T
Cuts at: 0 1342 1689
Size: 1342 347
KpnI G_GTAC'C
Cuts at: 0 510 1689
Size: 510 1179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1162 1689
Size: 926 236 527
Fragments arranged by size:
926 527 236
MscI TGG'CCA
Cuts at: 0 140 205 1689
Size: 140 65 1484
Fragments arranged by size:
1484 140 65
MslI CAynn'nnrTG
Cuts at: 0 941 1225 1570 1689
Size: 941 284 345 119
Fragments arranged by size:
941 345 284 119
MspA1I CmG'CkG
Cuts at: 0 18 1176 1211 1305 1689
Size: 18 1158 35 94 384
Fragments arranged by size:
1158 384 94 35 18
NdeI CA'TA_TG
Cuts at: 0 849 1689
Size: 849 840
NsiI A_TGCA'T
Cuts at: 0 1232 1689
Size: 1232 457
NspI r_CATG'y
Cuts at: 0 1575 1689
Size: 1575 114
PasI CC'CwG_GG
Cuts at: 0 785 1689
Size: 785 904
PflMI CCAn_nnn'nTGG
Cuts at: 0 177 785 1689
Size: 177 608 904
Fragments arranged by size:
904 608 177
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1477 1509 1689
Size: 1477 32 180
Fragments arranged by size:
1477 180 32
PpuMI rG'GwC_Cy
Cuts at: 0 295 1689
Size: 295 1394
PstI C_TGCA'G
Cuts at: 0 1038 1469 1689
Size: 1038 431 220
Fragments arranged by size:
1038 431 220
PvuII CAG'CTG
Cuts at: 0 1176 1211 1305 1689
Size: 1176 35 94 384
Fragments arranged by size:
1176 384 94 35
SapI GCTCTTCn'nnn_
Cuts at: 0 1109 1689
Size: 1109 580
SexAI A'CCwGG_T
Cuts at: 0 596 1487 1689
Size: 596 891 202
Fragments arranged by size:
891 596 202
SfcI C'TryA_G
Cuts at: 0 714 1034 1171 1465 1689
Size: 714 320 137 294 224
Fragments arranged by size:
714 320 294 224 137
SmlI C'TyrA_G
Cuts at: 0 163 706 1689
Size: 163 543 983
Fragments arranged by size:
983 543 163
SphI G_CATG'C
Cuts at: 0 1575 1689
Size: 1575 114
StuI AGG'CCT
Cuts at: 0 129 1689
Size: 129 1560
StyI C'CwwG_G
Cuts at: 0 449 869 953 1550 1689
Size: 449 420 84 597 139
Fragments arranged by size:
597 449 420 139 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 270 274 442 653 1423 1689
Size: 270 4 168 211 770 266
Fragments arranged by size:
770 270 266 211 168 4
TatI w'GTAC_w
Cuts at: 0 629 1689
Size: 629 1060
Tth111I GACn'n_nGTC
Cuts at: 0 1351 1689
Size: 1351 338
XmnI GAAnn'nnTTC
Cuts at: 0 813 1689
Size: 813 876
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlfI AloI AlwNI AvaI
BanI BanII BbvCI BciVI BclI BfrBI BglI BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsmI BsmBI Bsp1286I
BsrBI BssSI BstEII BstXI BtgI BtsI CspCI DraIII
DrdI EaeI EarI Eco57MI EcoO109I FspI HaeII Hin4I
HindIII KpnI MmeI MscI MslI MspA1I NdeI NsiI
NspI PasI PflMI PpiI PpuMI PstI PvuII SapI
SexAI SfcI SmlI SphI StuI StyI TaqII TatI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BcgI BglII BlpI Bme1580I BmtI
BsaAI BsgI BsiHKAI BsiWI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstYI BstZ17I Bsu36I
BtgZI ClaI DraI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI HincII HpaI KasI MfeI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PciI PfoI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI TstI XbaI XcmI XhoI
XmaI ZraI