Restriction map for
pFN21AE5684
(Linear) (Six-base) MAPSORT of: KIEE5684.seq Check: 8998 from: 1 to: 1725
KIEE5684.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AatII G_ACGT'C
Cuts at: 0 1414 1725
Size: 1414 311
Acc65I G'GTAC_C
Cuts at: 0 671 1725
Size: 671 1054
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 404 572 1725
Size: 154 250 168 1153
Fragments arranged by size:
1153 250 168 154
AfeI AGC'GCT
Cuts at: 0 1300 1725
Size: 1300 425
AlwNI CAG_nnn'CTG
Cuts at: 0 177 1725
Size: 177 1548
ApaI G_GGCC'C
Cuts at: 0 62 220 1311 1725
Size: 62 158 1091 414
Fragments arranged by size:
1091 414 158 62
ApaLI G'TGCA_C
Cuts at: 0 1105 1183 1725
Size: 1105 78 542
Fragments arranged by size:
1105 542 78
AvaI C'yCGr_G
Cuts at: 0 580 737 1110 1189 1302 1725
Size: 580 157 373 79 113 423
Fragments arranged by size:
580 423 373 157 113 79
BanI G'GyrC_C
Cuts at: 0 534 568 615 671 948 1120 1725
Size: 534 34 47 56 277 172 605
Fragments arranged by size:
605 534 277 172 56 47 34
BanII G_rGCy'C
Cuts at: 0 62 220 237 305 502 580 1117 1246
Size: 62 158 17 68 197 78 537 129
Cuts at: 1246 1311 1725
Size: 65 414
Fragments arranged by size:
537 414 197 158 129 78 68 65
62 17
BbeI G_GCGC'C
Cuts at: 0 1124 1725
Size: 1124 601
BbsI GAAGACnn'nnnn_
Cuts at: 0 644 1725
Size: 644 1081
BbvCI CC'TCA_GC
Cuts at: 0 1312 1725
Size: 1312 413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1545 1579 1725
Size: 1545 34 146
Fragments arranged by size:
1545 146 34
BglI GCCn_nnn'nGGC
Cuts at: 0 742 1100 1725
Size: 742 358 625
Fragments arranged by size:
742 625 358
BlpI GC'TnA_GC
Cuts at: 0 479 1295 1725
Size: 479 816 430
Fragments arranged by size:
816 479 430
Bme1580I G_kGCm'C
Cuts at: 0 62 220 573 1109 1187 1311 1725
Size: 62 158 353 536 78 124 414
Fragments arranged by size:
536 414 353 158 124 78 62
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 489 1725
Size: 489 1236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 547 1725
Size: 23 524 1178
Fragments arranged by size:
1178 524 23
Bpu10I CC'TnA_GC
Cuts at: 0 1312 1725
Size: 1312 413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 1725
Size: 486 1239
BsaI GGTCTCn'nnnn_
Cuts at: 0 577 769 1725
Size: 577 192 956
Fragments arranged by size:
956 577 192
BsaAI yAC'GTr
Cuts at: 0 993 1725
Size: 993 732
BsaHI Gr'CG_yC
Cuts at: 0 1010 1121 1411 1725
Size: 1010 111 290 314
Fragments arranged by size:
1010 314 290 111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1141 1171 1725
Size: 1141 30 554
Fragments arranged by size:
1141 554 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1516 1725
Size: 1516 209
BseYI C'CCAG_C
Cuts at: 0 336 686 779 1013 1090 1238 1488 1725
Size: 336 350 93 234 77 148 250 237
Fragments arranged by size:
350 336 250 237 234 148 93 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1264 1725
Size: 202 1062 461
Fragments arranged by size:
1062 461 202
BsiEI CG_ry'CG
Cuts at: 0 966 1725
Size: 966 759
BsiHKAI G_wGCw'C
Cuts at: 0 502 1109 1187 1725
Size: 502 607 78 538
Fragments arranged by size:
607 538 502 78
BsmI GAATG_Cn'
Cuts at: 0 364 1725
Size: 364 1361
BsmBI CGTCTCn'nnnn_
Cuts at: 0 523 813 1390 1725
Size: 523 290 577 335
Fragments arranged by size:
577 523 335 290
Bsp1286I G_dGCh'C
Cuts at: 0 62 220 237 305 502 573 580 1109
Size: 62 158 17 68 197 71 7 529
Cuts at: 1109 1117 1187 1246 1311 1725
Size: 8 70 59 65 414
Fragments arranged by size:
529 414 197 158 71 70 68 65
62 59 17 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 1283 1725
Size: 341 942 442
Fragments arranged by size:
942 442 341
BsrDI GCAATG_nn'
Cuts at: 0 251 1725
Size: 251 1474
BsrFI r'CCGG_y
Cuts at: 0 945 1466 1725
Size: 945 521 259
Fragments arranged by size:
945 521 259
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1517 1725
Size: 1517 208
BstXI CCAn_nnnn'nTGG
Cuts at: 0 51 675 690 1033 1098 1356 1725
Size: 51 624 15 343 65 258 369
Fragments arranged by size:
624 369 343 258 65 51 15
Bsu36I CC'TnA_GG
Cuts at: 0 425 1725
Size: 425 1300
BtgI C'CryG_G
Cuts at: 0 44 264 297 746 960 1530 1725
Size: 44 220 33 449 214 570 195
Fragments arranged by size:
570 449 220 214 195 44 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1005 1251 1524 1725
Size: 1005 246 273 201
Fragments arranged by size:
1005 273 246 201
BtsI GCAGTG_nn'
Cuts at: 0 348 1434 1725
Size: 348 1086 291
Fragments arranged by size:
1086 348 291
DrdI GACnn_nn'nnGTC
Cuts at: 0 805 1725
Size: 805 920
EaeI y'GGCC_r
Cuts at: 0 321 963 1035 1566 1604 1725
Size: 321 642 72 531 38 121
Fragments arranged by size:
642 531 321 121 72 38
EagI C'GGCC_G
Cuts at: 0 963 1725
Size: 963 762
EarI CTCTTCn'nnn_
Cuts at: 0 380 548 1725
Size: 380 168 1177
Fragments arranged by size:
1177 380 168
EcoICRI GAG'CTC
Cuts at: 0 500 1725
Size: 500 1225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 154 404 547 572 1725
Size: 23 131 250 143 25 1153
Fragments arranged by size:
1153 250 143 131 25 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 119 1725
Size: 119 1606
EcoO109I rG'GnC_Cy
Cuts at: 0 34 58 87 111 127 429 470 762
Size: 34 24 29 24 16 302 41 292
Cuts at: 762 1143 1209 1288 1307 1308 1725
Size: 381 66 79 19 1 417
Fragments arranged by size:
417 381 302 292 79 66 41 34
29 24 24 19 16 1
HaeII r_GCGC'y
Cuts at: 0 1124 1302 1725
Size: 1124 178 423
Fragments arranged by size:
1124 423 178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 522 554 1141 1173 1387 1419
Size: 433 32 57 32 587 32 214 32
Cuts at: 1419 1725
Size: 306
Fragments arranged by size:
587 433 306 214 57 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 1120 1725
Size: 1120 605
KpnI G_GTAC'C
Cuts at: 0 675 1725
Size: 675 1050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 677 1184 1725
Size: 124 553 507 541
Fragments arranged by size:
553 541 507 124
MscI TGG'CCA
Cuts at: 0 1037 1606 1725
Size: 1037 569 119
Fragments arranged by size:
1037 569 119
MslI CAynn'nnrTG
Cuts at: 0 73 724 1725
Size: 73 651 1001
Fragments arranged by size:
1001 651 73
MspA1I CmG'CkG
Cuts at: 0 565 690 838 1387 1488 1678 1725
Size: 565 125 148 549 101 190 47
Fragments arranged by size:
565 549 190 148 125 101 47
NaeI GCC'GGC
Cuts at: 0 947 1725
Size: 947 778
NarI GG'CG_CC
Cuts at: 0 1121 1725
Size: 1121 604
NcoI C'CATG_G
Cuts at: 0 44 264 746 1725
Size: 44 220 482 979
Fragments arranged by size:
979 482 220 44
NgoMIV G'CCGG_C
Cuts at: 0 945 1725
Size: 945 780
PasI CC'CwG_GG
Cuts at: 0 62 1725
Size: 62 1663
PflMI CCAn_nnn'nTGG
Cuts at: 0 270 565 1725
Size: 270 295 1160
Fragments arranged by size:
1160 295 270
PfoI T'CCnGG_A
Cuts at: 0 644 1725
Size: 644 1081
PpuMI rG'GwC_Cy
Cuts at: 0 87 111 429 1143 1725
Size: 87 24 318 714 582
Fragments arranged by size:
714 582 318 87 24
PspOMI G'GGCC_C
Cuts at: 0 58 216 1307 1725
Size: 58 158 1091 418
Fragments arranged by size:
1091 418 158 58
PstI C_TGCA'G
Cuts at: 0 1522 1725
Size: 1522 203
PvuII CAG'CTG
Cuts at: 0 690 1488 1678 1725
Size: 690 798 190 47
Fragments arranged by size:
798 690 190 47
SacI G_AGCT'C
Cuts at: 0 502 1725
Size: 502 1223
SanDI GG'GwC_CC
Cuts at: 0 111 1725
Size: 111 1614
SapI GCTCTTCn'nnn_
Cuts at: 0 380 548 1725
Size: 380 168 1177
Fragments arranged by size:
1177 380 168
SfcI C'TryA_G
Cuts at: 0 1056 1518 1725
Size: 1056 462 207
Fragments arranged by size:
1056 462 207
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 742 1725
Size: 742 983
SfoI GGC'GCC
Cuts at: 0 1122 1725
Size: 1122 603
SmaI CCC'GGG
Cuts at: 0 739 1725
Size: 739 986
SmlI C'TyrA_G
Cuts at: 0 501 1189 1725
Size: 501 688 536
Fragments arranged by size:
688 536 501
StuI AGG'CCT
Cuts at: 0 1042 1645 1725
Size: 1042 603 80
Fragments arranged by size:
1042 603 80
StyI C'CwwG_G
Cuts at: 0 44 264 746 1725
Size: 44 220 482 979
Fragments arranged by size:
979 482 220 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 450 700 1725
Size: 450 250 1025
Fragments arranged by size:
1025 450 250
Tth111I GACn'n_nGTC
Cuts at: 0 652 754 1401 1725
Size: 652 102 647 324
Fragments arranged by size:
652 647 324 102
XbaI T'CTAG_A
Cuts at: 0 393 1725
Size: 393 1332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 616 723 792 1135 1725
Size: 616 107 69 343 590
Fragments arranged by size:
616 590 343 107 69
XhoI C'TCGA_G
Cuts at: 0 1189 1725
Size: 1189 536
XmaI C'CCGG_G
Cuts at: 0 737 1725
Size: 737 988
ZraI GAC'GTC
Cuts at: 0 1412 1725
Size: 1412 313
Enzymes that do cut:
AatII Acc65I AcuI AfeI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrDI BsrFI BstAPI BstXI Bsu36I BtgI
BtgZI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SanDI SapI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BfrBI BglII BmgBI BmrI BmtI
BsaBI BsaWI BsiWI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstYI BstZ17I ClaI CspCI DraI
DraIII EciI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI MfeI MluI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI XmnI