Restriction map for
pFN21AE5682
(Linear) (Six-base) MAPSORT of: KIEE5682.seq Check: 7561 from: 1 to: 1644
KIEE5682.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 612 1644
Size: 612 1032
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 362 981 1525 1644
Size: 29 333 619 544 119
Fragments arranged by size:
619 544 333 119 29
AlwNI CAG_nnn'CTG
Cuts at: 0 169 1644
Size: 169 1475
ApoI r'AATT_y
Cuts at: 0 581 1644
Size: 581 1063
BamHI G'GATC_C
Cuts at: 0 144 1644
Size: 144 1500
BanI G'GyrC_C
Cuts at: 0 1094 1563 1644
Size: 1094 469 81
Fragments arranged by size:
1094 469 81
BanII G_rGCy'C
Cuts at: 0 534 791 1158 1175 1358 1463 1644
Size: 534 257 367 17 183 105 181
Fragments arranged by size:
534 367 257 183 181 105 17
BbeI G_GCGC'C
Cuts at: 0 1567 1644
Size: 1567 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 1644
Size: 10 1634
BbvCI CC'TCA_GC
Cuts at: 0 708 1644
Size: 708 936
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 824 858 1236 1270 1644
Size: 824 34 378 34 374
Fragments arranged by size:
824 378 374 34 34
BclI T'GATC_A
Cuts at: 0 1587 1644
Size: 1587 57
BglI GCCn_nnn'nGGC
Cuts at: 0 974 1644
Size: 974 670
BlpI GC'TnA_GC
Cuts at: 0 180 1169 1644
Size: 180 989 475
Fragments arranged by size:
989 475 180
Bme1580I G_kGCm'C
Cuts at: 0 1097 1644
Size: 1097 547
BmgBI CAC'GTC
Cuts at: 0 1521 1644
Size: 1521 123
BmrI ACTGGGnnnn_n'
Cuts at: 0 208 1644
Size: 208 1436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 803 1557 1644
Size: 47 756 754 87
Fragments arranged by size:
756 754 87 47
Bpu10I CC'TnA_GC
Cuts at: 0 708 1457 1644
Size: 708 749 187
Fragments arranged by size:
749 708 187
BsaAI yAC'GTr
Cuts at: 0 732 1644
Size: 732 912
BsaBI GATnn'nnATC
Cuts at: 0 763 1644
Size: 763 881
BsaHI Gr'CG_yC
Cuts at: 0 1564 1644
Size: 1564 80
BsaWI w'CCGG_w
Cuts at: 0 30 890 1438 1644
Size: 30 860 548 206
Fragments arranged by size:
860 548 206 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 1024 1054 1345 1375 1644
Size: 433 30 561 30 291 30 269
Fragments arranged by size:
561 433 291 269 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 1501 1644
Size: 295 1206 143
Fragments arranged by size:
1206 295 143
BseYI C'CCAG_C
Cuts at: 0 57 452 713 869 1002 1007 1086 1181
Size: 57 395 261 156 133 5 79 95
Cuts at: 1181 1491 1644
Size: 310 153
Fragments arranged by size:
395 310 261 156 153 133 95 79
57 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1371 1390 1644
Size: 1371 19 254
Fragments arranged by size:
1371 254 19
BsiEI CG_ry'CG
Cuts at: 0 1038 1271 1644
Size: 1038 233 373
Fragments arranged by size:
1038 373 233
BsiHKAI G_wGCw'C
Cuts at: 0 791 1175 1644
Size: 791 384 469
Fragments arranged by size:
791 469 384
BsmI GAATG_Cn'
Cuts at: 0 775 1644
Size: 775 869
Bsp1286I G_dGCh'C
Cuts at: 0 534 791 1097 1158 1175 1358 1463 1644
Size: 534 257 306 61 17 183 105 181
Fragments arranged by size:
534 306 257 183 181 105 61 17
BspEI T'CCGG_A
Cuts at: 0 30 1644
Size: 30 1614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 599 612 1644
Size: 599 13 1032
Fragments arranged by size:
1032 599 13
BsrBI CCG'CTC
Cuts at: 0 974 1040 1301 1644
Size: 974 66 261 343
Fragments arranged by size:
974 343 261 66
BsrDI GCAATG_nn'
Cuts at: 0 1344 1644
Size: 1344 300
BssSI C'ACGA_G
Cuts at: 0 1296 1644
Size: 1296 348
BstBI TT'CG_AA
Cuts at: 0 654 1644
Size: 654 990
BstEII G'GTnAC_C
Cuts at: 0 1201 1230 1644
Size: 1201 29 414
Fragments arranged by size:
1201 414 29
BstXI CCAn_nnnn'nTGG
Cuts at: 0 234 1644
Size: 234 1410
BstYI r'GATC_y
Cuts at: 0 144 165 1185 1644
Size: 144 21 1020 459
Fragments arranged by size:
1020 459 144 21
BtgI C'CryG_G
Cuts at: 0 383 911 1644
Size: 383 528 733
Fragments arranged by size:
733 528 383
BtsI GCAGTG_nn'
Cuts at: 0 1430 1644
Size: 1430 214
DrdI GACnn_nn'nnGTC
Cuts at: 0 937 1644
Size: 937 707
EaeI y'GGCC_r
Cuts at: 0 734 924 1035 1427 1644
Size: 734 190 111 392 217
Fragments arranged by size:
734 392 217 190 111
EagI C'GGCC_G
Cuts at: 0 1035 1644
Size: 1035 609
EarI CTCTTCn'nnn_
Cuts at: 0 880 1644
Size: 880 764
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 137 1644
Size: 137 1507
EcoICRI GAG'CTC
Cuts at: 0 789 1173 1644
Size: 789 384 471
Fragments arranged by size:
789 471 384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 47 362 803 981 1525 1557 1644
Size: 29 18 315 441 178 544 32 87
Fragments arranged by size:
544 441 315 178 87 32 29 18
EcoO109I rG'GnC_Cy
Cuts at: 0 54 210 1644
Size: 54 156 1434
Fragments arranged by size:
1434 156 54
HaeII r_GCGC'y
Cuts at: 0 1567 1644
Size: 1567 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 1054 1086 1224 1256 1644
Size: 355 32 667 32 138 32 388
Fragments arranged by size:
667 388 355 138 32 32 32
KasI G'GCGC_C
Cuts at: 0 1563 1644
Size: 1563 81
MscI TGG'CCA
Cuts at: 0 736 1429 1644
Size: 736 693 215
Fragments arranged by size:
736 693 215
MslI CAynn'nnrTG
Cuts at: 0 821 1130 1644
Size: 821 309 514
Fragments arranged by size:
821 514 309
MspA1I CmG'CkG
Cuts at: 0 184 409 456 1644
Size: 184 225 47 1188
Fragments arranged by size:
1188 225 184 47
NarI GG'CG_CC
Cuts at: 0 1564 1644
Size: 1564 80
NcoI C'CATG_G
Cuts at: 0 383 1644
Size: 383 1261
PasI CC'CwG_GG
Cuts at: 0 1044 1218 1644
Size: 1044 174 426
Fragments arranged by size:
1044 426 174
PpuMI rG'GwC_Cy
Cuts at: 0 54 210 1644
Size: 54 156 1434
Fragments arranged by size:
1434 156 54
PshAI GACnn'nnGTC
Cuts at: 0 38 1644
Size: 38 1606
PsiI TTA'TAA
Cuts at: 0 587 1644
Size: 587 1057
PstI C_TGCA'G
Cuts at: 0 318 414 1644
Size: 318 96 1230
Fragments arranged by size:
1230 318 96
PvuII CAG'CTG
Cuts at: 0 184 456 1644
Size: 184 272 1188
Fragments arranged by size:
1188 272 184
SacI G_AGCT'C
Cuts at: 0 791 1175 1644
Size: 791 384 469
Fragments arranged by size:
791 469 384
SanDI GG'GwC_CC
Cuts at: 0 210 1644
Size: 210 1434
SapI GCTCTTCn'nnn_
Cuts at: 0 880 1644
Size: 880 764
SfcI C'TryA_G
Cuts at: 0 314 410 441 1644
Size: 314 96 31 1203
Fragments arranged by size:
1203 314 96 31
SfoI GGC'GCC
Cuts at: 0 1565 1644
Size: 1565 79
StyI C'CwwG_G
Cuts at: 0 383 1098 1644
Size: 383 715 546
Fragments arranged by size:
715 546 383
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 511 1126 1621 1644
Size: 511 615 495 23
Fragments arranged by size:
615 511 495 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 885 917 1644
Size: 885 32 727
Fragments arranged by size:
885 727 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 877 1644
Size: 877 767
Enzymes that do cut:
AarI AcuI AlwNI ApoI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrDI BssSI BstBI BstEII BstXI BstYI
BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I
NarI NcoI PasI PpuMI PshAI PsiI PstI PvuII
SacI SanDI SapI SfcI SfoI StyI TaqII TstI
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BciVI BfrBI BglII BmtI
BplI BpuEI BsaI BsiWI BsmBI BspHI BsrFI BsrGI
BssHII BstAPI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI
XmaI XmnI ZraI