Restriction map for
pFN21AE5677
(Linear) (Six-base) MAPSORT of: KIEE5677.seq Check: 4910 from: 1 to: 1737
KIEE5677.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AclI AA'CG_TT
Cuts at: 0 1388 1737
Size: 1388 349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 113 1537 1737
Size: 33 80 1424 200
Fragments arranged by size:
1424 200 80 33
AfeI AGC'GCT
Cuts at: 0 725 1737
Size: 725 1012
AflIII A'CryG_T
Cuts at: 0 215 1737
Size: 215 1522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 445 851 1438 1737
Size: 445 406 587 299
Fragments arranged by size:
587 445 406 299
AlwNI CAG_nnn'CTG
Cuts at: 0 1641 1737
Size: 1641 96
ApoI r'AATT_y
Cuts at: 0 1207 1737
Size: 1207 530
AseI AT'TA_AT
Cuts at: 0 656 1737
Size: 656 1081
BanII G_rGCy'C
Cuts at: 0 195 1737
Size: 195 1542
BclI T'GATC_A
Cuts at: 0 374 1737
Size: 374 1363
BfrBI ATG'CAT
Cuts at: 0 502 1737
Size: 502 1235
BglI GCCn_nnn'nGGC
Cuts at: 0 352 487 1737
Size: 352 135 1250
Fragments arranged by size:
1250 352 135
BlpI GC'TnA_GC
Cuts at: 0 388 1737
Size: 388 1349
Bme1580I G_kGCm'C
Cuts at: 0 1631 1737
Size: 1631 106
BmrI ACTGGGnnnn_n'
Cuts at: 0 543 1416 1737
Size: 543 873 321
Fragments arranged by size:
873 543 321
BsaAI yAC'GTr
Cuts at: 0 456 1737
Size: 456 1281
BsaBI GATnn'nnATC
Cuts at: 0 79 1568 1737
Size: 79 1489 169
Fragments arranged by size:
1489 169 79
BsaWI w'CCGG_w
Cuts at: 0 71 1737
Size: 71 1666
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 182 212 1235 1265 1737
Size: 182 30 1023 30 472
Fragments arranged by size:
1023 472 182 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 1013 1737
Size: 79 934 724
Fragments arranged by size:
934 724 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 386 514 913 1737
Size: 217 169 128 399 824
Fragments arranged by size:
824 399 217 169 128
BsiEI CG_ry'CG
Cuts at: 0 411 1737
Size: 411 1326
BsiHKAI G_wGCw'C
Cuts at: 0 11 195 528 1737
Size: 11 184 333 1209
Fragments arranged by size:
1209 333 184 11
Bsp1286I G_dGCh'C
Cuts at: 0 11 195 528 1631 1737
Size: 11 184 333 1103 106
Fragments arranged by size:
1103 333 184 106 11
BspEI T'CCGG_A
Cuts at: 0 71 1737
Size: 71 1666
BspHI T'CATG_A
Cuts at: 0 1050 1737
Size: 1050 687
BsrDI GCAATG_nn'
Cuts at: 0 568 1737
Size: 568 1169
BsrFI r'CCGG_y
Cuts at: 0 518 1737
Size: 518 1219
BssSI C'ACGA_G
Cuts at: 0 194 1737
Size: 194 1543
BstYI r'GATC_y
Cuts at: 0 68 1737
Size: 68 1669
BtgI C'CryG_G
Cuts at: 0 582 945 1737
Size: 582 363 792
Fragments arranged by size:
792 582 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 717 1737
Size: 717 1020
BtsI GCAGTG_nn'
Cuts at: 0 11 229 399 925 1642 1737
Size: 11 218 170 526 717 95
Fragments arranged by size:
717 526 218 170 95 11
EaeI y'GGCC_r
Cuts at: 0 140 450 479 1737
Size: 140 310 29 1258
Fragments arranged by size:
1258 310 140 29
EarI CTCTTCn'nnn_
Cuts at: 0 137 335 1737
Size: 137 198 1402
Fragments arranged by size:
1402 198 137
EcoICRI GAG'CTC
Cuts at: 0 193 1737
Size: 193 1544
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 113 1537 1737
Size: 33 80 1424 200
Fragments arranged by size:
1424 200 80 33
EcoO109I rG'GnC_Cy
Cuts at: 0 1316 1737
Size: 1316 421
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1482 1737
Size: 1482 255
HaeII r_GCGC'y
Cuts at: 0 727 1737
Size: 727 1010
HincII GTy'rAC
Cuts at: 0 765 897 1737
Size: 765 132 840
Fragments arranged by size:
840 765 132
HpaI GTT'AAC
Cuts at: 0 765 897 1737
Size: 765 132 840
Fragments arranged by size:
840 765 132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 1737
Size: 431 1306
MscI TGG'CCA
Cuts at: 0 481 1737
Size: 481 1256
MslI CAynn'nnrTG
Cuts at: 0 557 1737
Size: 557 1180
MspA1I CmG'CkG
Cuts at: 0 53 292 940 1082 1280 1336 1435 1737
Size: 53 239 648 142 198 56 99 302
Fragments arranged by size:
648 302 239 198 142 99 56 53
NcoI C'CATG_G
Cuts at: 0 582 945 1737
Size: 582 363 792
Fragments arranged by size:
792 582 363
NsiI A_TGCA'T
Cuts at: 0 504 1737
Size: 504 1233
NspI r_CATG'y
Cuts at: 0 219 634 1119 1737
Size: 219 415 485 618
Fragments arranged by size:
618 485 415 219
PciI A'CATG_T
Cuts at: 0 215 1737
Size: 215 1522
PfoI T'CCnGG_A
Cuts at: 0 1580 1737
Size: 1580 157
PmlI CAC'GTG
Cuts at: 0 456 1737
Size: 456 1281
PpuMI rG'GwC_Cy
Cuts at: 0 1316 1737
Size: 1316 421
PshAI GACnn'nnGTC
Cuts at: 0 940 1737
Size: 940 797
PstI C_TGCA'G
Cuts at: 0 639 1165 1242 1737
Size: 639 526 77 495
Fragments arranged by size:
639 526 495 77
PvuII CAG'CTG
Cuts at: 0 292 940 1336 1737
Size: 292 648 396 401
Fragments arranged by size:
648 401 396 292
SacI G_AGCT'C
Cuts at: 0 195 1737
Size: 195 1542
SfcI C'TryA_G
Cuts at: 0 635 1161 1238 1737
Size: 635 526 77 499
Fragments arranged by size:
635 526 499 77
SphI G_CATG'C
Cuts at: 0 634 1737
Size: 634 1103
StyI C'CwwG_G
Cuts at: 0 206 582 945 1737
Size: 206 376 363 792
Fragments arranged by size:
792 376 363 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 425 1737
Size: 425 1312
TatI w'GTAC_w
Cuts at: 0 267 1737
Size: 267 1470
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 202 234 1235 1267 1737
Size: 202 32 1001 32 470
Fragments arranged by size:
1001 470 202 32 32
XbaI T'CTAG_A
Cuts at: 0 1542 1737
Size: 1542 195
Enzymes that do cut:
AclI AcuI AfeI AflIII AlfI AlwNI ApoI AseI
BanII BclI BfrBI BglI BlpI Bme1580I BmrI BsaAI
BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BsrDI BsrFI BssSI BstYI BtgI BtgZI
BtsI EaeI EarI EcoICRI Eco57MI EcoO109I FalI HaeII
HincII HpaI MmeI MscI MslI MspA1I NcoI NsiI
NspI PciI PfoI PmlI PpuMI PshAI PstI PvuII
SacI SfcI SphI StyI TaqII TatI TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BciVI BglII
BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BseYI BsiWI BsmI BsmBI BspMI BsrBI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI Hin4I HindIII KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PmeI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XcmI
XhoI XmaI XmnI ZraI