Restriction map for
pFN21AE5676
(Linear) (Six-base) MAPSORT of: KIEE5676.seq Check: 582 from: 1 to: 1737
KIEE5676.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 926 1130 1737
Size: 380 546 204 607
Fragments arranged by size:
607 546 380 204
AflIII A'CryG_T
Cuts at: 0 328 411 874 1737
Size: 328 83 463 863
Fragments arranged by size:
863 463 328 83
AleI CACnn'nnGTG
Cuts at: 0 1436 1737
Size: 1436 301
AlwNI CAG_nnn'CTG
Cuts at: 0 622 1737
Size: 622 1115
ApoI r'AATT_y
Cuts at: 0 174 267 1258 1737
Size: 174 93 991 479
Fragments arranged by size:
991 479 174 93
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1166 1199 1737
Size: 1166 33 538
Fragments arranged by size:
1166 538 33
BanII G_rGCy'C
Cuts at: 0 32 1737
Size: 32 1705
BbsI GAAGACnn'nnnn_
Cuts at: 0 386 698 724 1737
Size: 386 312 26 1013
Fragments arranged by size:
1013 386 312 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 679 713 1737
Size: 679 34 1024
Fragments arranged by size:
1024 679 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1207 1737
Size: 1207 530
BclI T'GATC_A
Cuts at: 0 1269 1737
Size: 1269 468
BglII A'GATC_T
Cuts at: 0 483 668 1476 1737
Size: 483 185 808 261
Fragments arranged by size:
808 483 261 185
BlpI GC'TnA_GC
Cuts at: 0 22 1737
Size: 22 1715
Bme1580I G_kGCm'C
Cuts at: 0 843 1737
Size: 843 894
BmrI ACTGGGnnnn_n'
Cuts at: 0 720 1049 1737
Size: 720 329 688
Fragments arranged by size:
720 688 329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1439 1737
Size: 409 1030 298
Fragments arranged by size:
1030 409 298
Bpu10I CC'TnA_GC
Cuts at: 0 615 1737
Size: 615 1122
BsaBI GATnn'nnATC
Cuts at: 0 1238 1737
Size: 1238 499
BsaWI w'CCGG_w
Cuts at: 0 87 1737
Size: 87 1650
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 823 1737
Size: 823 914
BsiHKAI G_wGCw'C
Cuts at: 0 515 616 1737
Size: 515 101 1121
Fragments arranged by size:
1121 515 101
BsmI GAATG_Cn'
Cuts at: 0 979 1737
Size: 979 758
Bsp1286I G_dGCh'C
Cuts at: 0 32 515 616 843 1737
Size: 32 483 101 227 894
Fragments arranged by size:
894 483 227 101 32
BspEI T'CCGG_A
Cuts at: 0 87 1737
Size: 87 1650
BsrDI GCAATG_nn'
Cuts at: 0 1002 1247 1401 1737
Size: 1002 245 154 336
Fragments arranged by size:
1002 336 245 154
BsrFI r'CCGG_y
Cuts at: 0 889 1737
Size: 889 848
BsrGI T'GTAC_A
Cuts at: 0 1108 1737
Size: 1108 629
BstBI TT'CG_AA
Cuts at: 0 689 1737
Size: 689 1048
BstXI CCAn_nnnn'nTGG
Cuts at: 0 531 1589 1737
Size: 531 1058 148
Fragments arranged by size:
1058 531 148
BstYI r'GATC_y
Cuts at: 0 483 668 753 1067 1476 1737
Size: 483 185 85 314 409 261
Fragments arranged by size:
483 409 314 261 185 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1715 1737
Size: 1715 22
BtsI GCAGTG_nn'
Cuts at: 0 782 1737
Size: 782 955
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 293 328 1273 1308 1737
Size: 293 35 945 35 429
Fragments arranged by size:
945 429 293 35 35
DraI TTT'AAA
Cuts at: 0 495 885 1737
Size: 495 390 852
Fragments arranged by size:
852 495 390
DraIII CAC_nnn'GTG
Cuts at: 0 1223 1225 1737
Size: 1223 2 512
Fragments arranged by size:
1223 512 2
EaeI y'GGCC_r
Cuts at: 0 1591 1737
Size: 1591 146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 409 926 1130 1439 1737
Size: 380 29 517 204 309 298
Fragments arranged by size:
517 380 309 298 204 29
HaeII r_GCGC'y
Cuts at: 0 1554 1737
Size: 1554 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 460 492 1029 1061 1737
Size: 460 32 537 32 676
Fragments arranged by size:
676 537 460 32 32
MscI TGG'CCA
Cuts at: 0 1593 1737
Size: 1593 144
MslI CAynn'nnrTG
Cuts at: 0 656 1049 1252 1436 1737
Size: 656 393 203 184 301
Fragments arranged by size:
656 393 301 203 184
MspA1I CmG'CkG
Cuts at: 0 26 1737
Size: 26 1711
NspI r_CATG'y
Cuts at: 0 332 415 860 878 1737
Size: 332 83 445 18 859
Fragments arranged by size:
859 445 332 83 18
PciI A'CATG_T
Cuts at: 0 328 411 874 1737
Size: 328 83 463 863
Fragments arranged by size:
863 463 328 83
PstI C_TGCA'G
Cuts at: 0 685 1737
Size: 685 1052
SfcI C'TryA_G
Cuts at: 0 352 681 1434 1520 1737
Size: 352 329 753 86 217
Fragments arranged by size:
753 352 329 217 86
SspI AAT'ATT
Cuts at: 0 991 1737
Size: 991 746
StuI AGG'CCT
Cuts at: 0 960 1737
Size: 960 777
StyI C'CwwG_G
Cuts at: 0 282 1737
Size: 282 1455
TatI w'GTAC_w
Cuts at: 0 331 1108 1378 1632 1737
Size: 331 777 270 254 105
Fragments arranged by size:
777 331 270 254 105
XmnI GAAnn'nnTTC
Cuts at: 0 647 1737
Size: 647 1090
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApoI BaeI BanII BbsI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BsaBI BsaWI BseRI BsiHKAI BsmI Bsp1286I BspEI
BsrDI BsrFI BsrGI BstBI BstXI BstYI BtgZI BtsI
CspCI DraI DraIII EaeI Eco57MI HaeII Hin4I MscI
MslI MspA1I NspI PciI PstI SfcI SspI StuI
StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanI BbeI BbvCI BfrBI BglI
BmgBI BmtI BplI BpuEI BsaI BsaAI BsaHI BsaXI
BseYI BsgI BsiEI BsiWI BsmBI BspHI BspMI BsrBI
BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgI ClaI
DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
ZraI