Restriction map for
pFN21AE5675
(Linear) (Six-base) MAPSORT of: KIEE5675.seq Check: 6535 from: 1 to: 1674
KIEE5675.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AccI GT'mk_AC
Cuts at: 0 255 1077 1674
Size: 255 822 597
Fragments arranged by size:
822 597 255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1510 1674
Size: 673 837 164
Fragments arranged by size:
837 673 164
AflII C'TTAA_G
Cuts at: 0 424 1674
Size: 424 1250
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1076 1108 1674
Size: 1076 32 566
Fragments arranged by size:
1076 566 32
AlwNI CAG_nnn'CTG
Cuts at: 0 940 1674
Size: 940 734
ApaLI G'TGCA_C
Cuts at: 0 905 1674
Size: 905 769
ApoI r'AATT_y
Cuts at: 0 157 212 628 1086 1534 1674
Size: 157 55 416 458 448 140
Fragments arranged by size:
458 448 416 157 140 55
AvaI C'yCGr_G
Cuts at: 0 452 1331 1674
Size: 452 879 343
Fragments arranged by size:
879 452 343
BanII G_rGCy'C
Cuts at: 0 459 556 1011 1408 1674
Size: 459 97 455 397 266
Fragments arranged by size:
459 455 397 266 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 1352 1674
Size: 1352 322
BfrBI ATG'CAT
Cuts at: 0 757 1674
Size: 757 917
BglI GCCn_nnn'nGGC
Cuts at: 0 727 1674
Size: 727 947
BlpI GC'TnA_GC
Cuts at: 0 1241 1674
Size: 1241 433
Bme1580I G_kGCm'C
Cuts at: 0 909 1674
Size: 909 765
BmrI ACTGGGnnnn_n'
Cuts at: 0 697 1674
Size: 697 977
BmtI G_CTAG'C
Cuts at: 0 1249 1674
Size: 1249 425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1025 1674
Size: 533 492 649
Fragments arranged by size:
649 533 492
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 1674
Size: 956 718
BsaBI GATnn'nnATC
Cuts at: 0 1172 1232 1674
Size: 1172 60 442
Fragments arranged by size:
1172 442 60
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 362 1674
Size: 362 1312
BseYI C'CCAG_C
Cuts at: 0 954 1674
Size: 954 720
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 747 1674
Size: 449 298 927
Fragments arranged by size:
927 449 298
BsiHKAI G_wGCw'C
Cuts at: 0 909 1011 1674
Size: 909 102 663
Fragments arranged by size:
909 663 102
Bsp1286I G_dGCh'C
Cuts at: 0 459 556 909 1011 1408 1674
Size: 459 97 353 102 397 266
Fragments arranged by size:
459 397 353 266 102 97
BsrDI GCAATG_nn'
Cuts at: 0 1256 1674
Size: 1256 418
BsrGI T'GTAC_A
Cuts at: 0 1170 1674
Size: 1170 504
BstAPI GCAn_nnn'nTGC
Cuts at: 0 718 940 1674
Size: 718 222 734
Fragments arranged by size:
734 718 222
BstBI TT'CG_AA
Cuts at: 0 207 381 564 1674
Size: 207 174 183 1110
Fragments arranged by size:
1110 207 183 174
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1264 1627 1674
Size: 1264 363 47
Fragments arranged by size:
1264 363 47
BstYI r'GATC_y
Cuts at: 0 64 1576 1674
Size: 64 1512 98
Fragments arranged by size:
1512 98 64
BstZ17I GTA'TAC
Cuts at: 0 1078 1674
Size: 1078 596
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1372 1674
Size: 1372 302
BtsI GCAGTG_nn'
Cuts at: 0 466 1674
Size: 466 1208
DraI TTT'AAA
Cuts at: 0 162 1395 1533 1674
Size: 162 1233 138 141
Fragments arranged by size:
1233 162 141 138
EaeI y'GGCC_r
Cuts at: 0 738 864 1055 1674
Size: 738 126 191 619
Fragments arranged by size:
738 619 191 126
EarI CTCTTCn'nnn_
Cuts at: 0 1038 1674
Size: 1038 636
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1240 1674
Size: 1240 434
EcoICRI GAG'CTC
Cuts at: 0 1009 1674
Size: 1009 665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 673 1025 1510 1674
Size: 533 140 352 485 164
Fragments arranged by size:
533 485 352 164 140
EcoO109I rG'GnC_Cy
Cuts at: 0 517 1599 1674
Size: 517 1082 75
Fragments arranged by size:
1082 517 75
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 323 703 1674
Size: 323 380 971
Fragments arranged by size:
971 380 323
FspI TGC'GCA
Cuts at: 0 772 1674
Size: 772 902
HaeII r_GCGC'y
Cuts at: 0 1564 1674
Size: 1564 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 420 452 1674
Size: 229 32 159 32 1222
Fragments arranged by size:
1222 229 159 32 32
MfeI C'AATT_G
Cuts at: 0 1570 1674
Size: 1570 104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1481 1674
Size: 1481 193
MscI TGG'CCA
Cuts at: 0 740 866 1057 1674
Size: 740 126 191 617
Fragments arranged by size:
740 617 191 126
MslI CAynn'nnrTG
Cuts at: 0 1262 1619 1674
Size: 1262 357 55
Fragments arranged by size:
1262 357 55
MspA1I CmG'CkG
Cuts at: 0 997 1674
Size: 997 677
NdeI CA'TA_TG
Cuts at: 0 930 1674
Size: 930 744
NheI G'CTAG_C
Cuts at: 0 1245 1674
Size: 1245 429
NsiI A_TGCA'T
Cuts at: 0 759 1674
Size: 759 915
NspI r_CATG'y
Cuts at: 0 761 1674
Size: 761 913
PflMI CCAn_nnn'nTGG
Cuts at: 0 961 1674
Size: 961 713
PfoI T'CCnGG_A
Cuts at: 0 1002 1674
Size: 1002 672
PpuMI rG'GwC_Cy
Cuts at: 0 517 1674
Size: 517 1157
PshAI GACnn'nnGTC
Cuts at: 0 1352 1674
Size: 1352 322
PspXI vC'TCGA_Gb
Cuts at: 0 452 1674
Size: 452 1222
PstI C_TGCA'G
Cuts at: 0 473 1674
Size: 473 1201
PvuII CAG'CTG
Cuts at: 0 997 1674
Size: 997 677
SacI G_AGCT'C
Cuts at: 0 1011 1674
Size: 1011 663
SfcI C'TryA_G
Cuts at: 0 131 469 977 1674
Size: 131 338 508 697
Fragments arranged by size:
697 508 338 131
SmlI C'TyrA_G
Cuts at: 0 424 452 971 1331 1674
Size: 424 28 519 360 343
Fragments arranged by size:
519 424 360 343 28
SphI G_CATG'C
Cuts at: 0 761 1674
Size: 761 913
StyI C'CwwG_G
Cuts at: 0 1211 1350 1626 1674
Size: 1211 139 276 48
Fragments arranged by size:
1211 276 139 48
TatI w'GTAC_w
Cuts at: 0 1170 1320 1674
Size: 1170 150 354
Fragments arranged by size:
1170 354 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1505 1537 1674
Size: 1505 32 137
Fragments arranged by size:
1505 137 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 729 1674
Size: 729 945
XhoI C'TCGA_G
Cuts at: 0 452 1331 1674
Size: 452 879 343
Fragments arranged by size:
879 452 343
XmnI GAAnn'nnTTC
Cuts at: 0 608 1498 1592 1674
Size: 608 890 94 82
Fragments arranged by size:
890 608 94 82
Enzymes that do cut:
AccI AcuI AflII AloI AlwNI ApaLI ApoI AvaI
BanII BbsI BfrBI BglI BlpI Bme1580I BmrI BmtI
BpmI BpuEI BsaBI BseRI BseYI BsgI BsiHKAI Bsp1286I
BsrDI BsrGI BstAPI BstBI BstXI BstYI BstZ17I BtgZI
BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
FalI FspI HaeII Hin4I MfeI MmeI MscI MslI
MspA1I NdeI NheI NsiI NspI PflMI PfoI PpuMI
PshAI PspXI PstI PvuII SacI SfcI SmlI SphI
StyI TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BglII
BmgBI BplI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstEII Bsu36I BtgI ClaI CspCI
DraIII DrdI EagI EcoNI EcoRI EcoRV FseI FspAI
HincII HindIII HpaI KasI KpnI MluI NaeI NarI
NcoI NgoMIV NotI NruI PacI PasI PciI PmeI
PmlI PpiI PsiI PspOMI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XmaI ZraI