Restriction map for
pFN21AE5674
(Linear) (Six-base) MAPSORT of: KIEE5674.seq Check: 5508 from: 1 to: 1668
KIEE5674.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AccI GT'mk_AC
Cuts at: 0 1161 1668
Size: 1161 507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 685 1668
Size: 526 159 983
Fragments arranged by size:
983 526 159
AflII C'TTAA_G
Cuts at: 0 460 1668
Size: 460 1208
AflIII A'CryG_T
Cuts at: 0 1419 1668
Size: 1419 249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1479 1668
Size: 1479 189
AlwNI CAG_nnn'CTG
Cuts at: 0 960 1668
Size: 960 708
ApoI r'AATT_y
Cuts at: 0 76 435 445 916 977 1668
Size: 76 359 10 471 61 691
Fragments arranged by size:
691 471 359 76 61 10
AseI AT'TA_AT
Cuts at: 0 817 1668
Size: 817 851
AvaI C'yCGr_G
Cuts at: 0 1651 1668
Size: 1651 17
BanII G_rGCy'C
Cuts at: 0 274 1668
Size: 274 1394
BbsI GAAGACnn'nnnn_
Cuts at: 0 514 1604 1668
Size: 514 1090 64
Fragments arranged by size:
1090 514 64
BclI T'GATC_A
Cuts at: 0 1025 1229 1668
Size: 1025 204 439
Fragments arranged by size:
1025 439 204
BglII A'GATC_T
Cuts at: 0 546 1668
Size: 546 1122
BmgBI CAC'GTC
Cuts at: 0 311 1668
Size: 311 1357
BmrI ACTGGGnnnn_n'
Cuts at: 0 277 928 1668
Size: 277 651 740
Fragments arranged by size:
740 651 277
BmtI G_CTAG'C
Cuts at: 0 1631 1668
Size: 1631 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 176 1668
Size: 117 59 1492
Fragments arranged by size:
1492 117 59
Bpu10I CC'TnA_GC
Cuts at: 0 8 424 1668
Size: 8 416 1244
Fragments arranged by size:
1244 416 8
BsaWI w'CCGG_w
Cuts at: 0 405 1668
Size: 405 1263
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 633 663 696 726 1668
Size: 633 30 33 30 942
Fragments arranged by size:
942 633 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 855 1130 1668
Size: 37 818 275 538
Fragments arranged by size:
818 538 275 37
BseYI C'CCAG_C
Cuts at: 0 923 1566 1668
Size: 923 643 102
Fragments arranged by size:
923 643 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 954 1426 1449 1668
Size: 355 599 472 23 219
Fragments arranged by size:
599 472 355 219 23
Bsp1286I G_dGCh'C
Cuts at: 0 274 1668
Size: 274 1394
BspEI T'CCGG_A
Cuts at: 0 405 1668
Size: 405 1263
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1625 1668
Size: 1625 43
BsrDI GCAATG_nn'
Cuts at: 0 190 233 1478 1580 1668
Size: 190 43 1245 102 88
Fragments arranged by size:
1245 190 102 88 43
BsrGI T'GTAC_A
Cuts at: 0 414 794 1668
Size: 414 380 874
Fragments arranged by size:
874 414 380
BstAPI GCAn_nnn'nTGC
Cuts at: 0 586 1668
Size: 586 1082
BstYI r'GATC_y
Cuts at: 0 342 546 1660 1668
Size: 342 204 1114 8
Fragments arranged by size:
1114 342 204 8
BtgI C'CryG_G
Cuts at: 0 381 1668
Size: 381 1287
BtsI GCAGTG_nn'
Cuts at: 0 1113 1668
Size: 1113 555
DraIII CAC_nnn'GTG
Cuts at: 0 1242 1371 1668
Size: 1242 129 297
Fragments arranged by size:
1242 297 129
EaeI y'GGCC_r
Cuts at: 0 1152 1203 1668
Size: 1152 51 465
Fragments arranged by size:
1152 465 51
EarI CTCTTCn'nnn_
Cuts at: 0 130 1103 1148 1668
Size: 130 973 45 520
Fragments arranged by size:
973 520 130 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 176 526 685 1668
Size: 117 59 350 159 983
Fragments arranged by size:
983 350 159 117 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1129 1668
Size: 1129 539
EcoO109I rG'GnC_Cy
Cuts at: 0 465 1050 1668
Size: 465 585 618
Fragments arranged by size:
618 585 465
EcoRI G'AATT_C
Cuts at: 0 977 1668
Size: 977 691
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 36 68 633 665 696 728 1645 1668
Size: 36 32 565 32 31 32 917 23
Fragments arranged by size:
917 565 36 32 32 32 31 23
HincII GTy'rAC
Cuts at: 0 840 1668
Size: 840 828
HindIII A'AGCT_T
Cuts at: 0 112 1668
Size: 112 1556
MfeI C'AATT_G
Cuts at: 0 773 806 1668
Size: 773 33 862
Fragments arranged by size:
862 773 33
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 1668
Size: 1430 238
MslI CAynn'nnrTG
Cuts at: 0 281 617 1513 1668
Size: 281 336 896 155
Fragments arranged by size:
896 336 281 155
MspA1I CmG'CkG
Cuts at: 0 179 1668
Size: 179 1489
NcoI C'CATG_G
Cuts at: 0 381 1668
Size: 381 1287
NheI G'CTAG_C
Cuts at: 0 1627 1668
Size: 1627 41
NspI r_CATG'y
Cuts at: 0 1423 1668
Size: 1423 245
PciI A'CATG_T
Cuts at: 0 1419 1668
Size: 1419 249
PflMI CCAn_nnn'nTGG
Cuts at: 0 643 1668
Size: 643 1025
PfoI T'CCnGG_A
Cuts at: 0 56 1668
Size: 56 1612
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 935 967 1668
Size: 935 32 701
Fragments arranged by size:
935 701 32
PpuMI rG'GwC_Cy
Cuts at: 0 1050 1668
Size: 1050 618
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 33 65 652 684 1668
Size: 33 32 587 32 984
Fragments arranged by size:
984 587 33 32 32
PstI C_TGCA'G
Cuts at: 0 351 965 1668
Size: 351 614 703
Fragments arranged by size:
703 614 351
PvuII CAG'CTG
Cuts at: 0 179 1668
Size: 179 1489
SapI GCTCTTCn'nnn_
Cuts at: 0 1103 1668
Size: 1103 565
SbfI CC_TGCA'GG
Cuts at: 0 351 1668
Size: 351 1317
ScaI AGT'ACT
Cuts at: 0 670 1668
Size: 670 998
SfcI C'TryA_G
Cuts at: 0 347 961 1162 1668
Size: 347 614 201 506
Fragments arranged by size:
614 506 347 201
SmlI C'TyrA_G
Cuts at: 0 460 1668
Size: 460 1208
StyI C'CwwG_G
Cuts at: 0 381 596 637 1668
Size: 381 215 41 1031
Fragments arranged by size:
1031 381 215 41
TatI w'GTAC_w
Cuts at: 0 414 518 569 668 794 1668
Size: 414 104 51 99 126 874
Fragments arranged by size:
874 414 126 104 99 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 963 995 1668
Size: 963 32 673
Fragments arranged by size:
963 673 32
XbaI T'CTAG_A
Cuts at: 0 940 1668
Size: 940 728
XmnI GAAnn'nnTTC
Cuts at: 0 1550 1668
Size: 1550 118
Enzymes that do cut:
AccI AcuI AflII AflIII AlfI AlwNI ApoI AseI
AvaI BanII BbsI BclI BglII BmgBI BmrI BmtI
BpmI Bpu10I BsaWI BsaXI BseRI BseYI BsgI Bsp1286I
BspEI BspMI BsrDI BsrGI BstAPI BstYI BtgI BtsI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I
HincII HindIII MfeI MmeI MslI MspA1I NcoI NheI
NspI PciI PflMI PfoI PpiI PpuMI PsrI PstI
PvuII SapI SbfI ScaI SfcI SmlI StyI TatI
TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BfrBI BglI BlpI
Bme1580I BplI BpuEI BsaI BsaAI BsaBI BsaHI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
MscI NaeI NarI NdeI NgoMIV NotI NruI NsiI
PacI PasI PmeI PmlI PshAI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XcmI XhoI XmaI
ZraI