Restriction map for
pFN21AE5673
(Linear) (Six-base) MAPSORT of: KIEE5673.seq Check: 633 from: 1 to: 1629
KIEE5673.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
Acc65I G'GTAC_C
Cuts at: 0 962 1629
Size: 962 667
AclI AA'CG_TT
Cuts at: 0 884 1629
Size: 884 745
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1452 1629
Size: 1452 177
AleI CACnn'nnGTG
Cuts at: 0 457 1629
Size: 457 1172
AlwNI CAG_nnn'CTG
Cuts at: 0 471 1629
Size: 471 1158
ApoI r'AATT_y
Cuts at: 0 1088 1629
Size: 1088 541
BanI G'GyrC_C
Cuts at: 0 554 962 1629
Size: 554 408 667
Fragments arranged by size:
667 554 408
BanII G_rGCy'C
Cuts at: 0 126 254 1629
Size: 126 128 1375
Fragments arranged by size:
1375 128 126
BglI GCCn_nnn'nGGC
Cuts at: 0 1009 1629
Size: 1009 620
Bme1580I G_kGCm'C
Cuts at: 0 559 1377 1629
Size: 559 818 252
Fragments arranged by size:
818 559 252
BmgBI CAC'GTC
Cuts at: 0 1248 1629
Size: 1248 381
BmrI ACTGGGnnnn_n'
Cuts at: 0 904 1629
Size: 904 725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 448 451 970 1629
Size: 86 362 3 519 659
Fragments arranged by size:
659 519 362 86 3
Bpu10I CC'TnA_GC
Cuts at: 0 1124 1629
Size: 1124 505
BsaI GGTCTCn'nnnn_
Cuts at: 0 1033 1629
Size: 1033 596
BsaAI yAC'GTr
Cuts at: 0 108 1629
Size: 108 1521
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 1160 1629
Size: 151 1009 469
Fragments arranged by size:
1009 469 151
BseYI C'CCAG_C
Cuts at: 0 1538 1629
Size: 1538 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1058 1629
Size: 1058 571
BsiHKAI G_wGCw'C
Cuts at: 0 254 464 1629
Size: 254 210 1165
Fragments arranged by size:
1165 254 210
BsmI GAATG_Cn'
Cuts at: 0 341 658 1050 1170 1629
Size: 341 317 392 120 459
Fragments arranged by size:
459 392 341 317 120
BsmBI CGTCTCn'nnnn_
Cuts at: 0 404 1629
Size: 404 1225
Bsp1286I G_dGCh'C
Cuts at: 0 126 254 464 559 1377 1629
Size: 126 128 210 95 818 252
Fragments arranged by size:
818 252 210 128 126 95
BspHI T'CATG_A
Cuts at: 0 254 1629
Size: 254 1375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 704 1629
Size: 704 925
BsrGI T'GTAC_A
Cuts at: 0 312 1629
Size: 312 1317
BssHII G'CGCG_C
Cuts at: 0 30 1629
Size: 30 1599
BssSI C'ACGA_G
Cuts at: 0 157 1629
Size: 157 1472
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1045 1629
Size: 1045 584
BstXI CCAn_nnnn'nTGG
Cuts at: 0 812 1629
Size: 812 817
BstYI r'GATC_y
Cuts at: 0 681 1629
Size: 681 948
Bsu36I CC'TnA_GG
Cuts at: 0 1503 1561 1629
Size: 1503 58 68
Fragments arranged by size:
1503 68 58
BtgI C'CryG_G
Cuts at: 0 1433 1629
Size: 1433 196
BtsI GCAGTG_nn'
Cuts at: 0 549 1629
Size: 549 1080
DraI TTT'AAA
Cuts at: 0 1085 1629
Size: 1085 544
DraIII CAC_nnn'GTG
Cuts at: 0 1154 1629
Size: 1154 475
EaeI y'GGCC_r
Cuts at: 0 482 509 1010 1629
Size: 482 27 501 619
Fragments arranged by size:
619 501 482 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 38 1629
Size: 38 1591
EcoICRI GAG'CTC
Cuts at: 0 252 1629
Size: 252 1377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 448 451 970 1452 1629
Size: 86 362 3 519 482 177
Fragments arranged by size:
519 482 362 177 86 3
FspI TGC'GCA
Cuts at: 0 11 1629
Size: 11 1618
FspAI rTGC'GCAy
Cuts at: 0 11 1629
Size: 11 1618
HaeII r_GCGC'y
Cuts at: 0 471 1614 1629
Size: 471 1143 15
Fragments arranged by size:
1143 471 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 35 67 181 213 1406 1438 1584 1616
Size: 35 32 114 32 1193 32 146 32
Cuts at: 1616 1629
Size: 13
Fragments arranged by size:
1193 146 114 35 32 32 32 32
13
HincII GTy'rAC
Cuts at: 0 621 1629
Size: 621 1008
HindIII A'AGCT_T
Cuts at: 0 79 857 1629
Size: 79 778 772
Fragments arranged by size:
778 772 79
KpnI G_GTAC'C
Cuts at: 0 966 1629
Size: 966 663
MfeI C'AATT_G
Cuts at: 0 842 1629
Size: 842 787
MscI TGG'CCA
Cuts at: 0 484 511 1012 1629
Size: 484 27 501 617
Fragments arranged by size:
617 501 484 27
MslI CAynn'nnrTG
Cuts at: 0 457 542 1139 1366 1629
Size: 457 85 597 227 263
Fragments arranged by size:
597 457 263 227 85
NspI r_CATG'y
Cuts at: 0 10 734 796 1629
Size: 10 724 62 833
Fragments arranged by size:
833 724 62 10
PasI CC'CwG_GG
Cuts at: 0 117 1317 1629
Size: 117 1200 312
Fragments arranged by size:
1200 312 117
PflMI CCAn_nnn'nTGG
Cuts at: 0 337 565 972 1154 1629
Size: 337 228 407 182 475
Fragments arranged by size:
475 407 337 228 182
PsiI TTA'TAA
Cuts at: 0 997 1629
Size: 997 632
PstI C_TGCA'G
Cuts at: 0 300 1629
Size: 300 1329
SacI G_AGCT'C
Cuts at: 0 254 1629
Size: 254 1375
ScaI AGT'ACT
Cuts at: 0 1473 1629
Size: 1473 156
SfcI C'TryA_G
Cuts at: 0 296 1629
Size: 296 1333
SphI G_CATG'C
Cuts at: 0 10 1629
Size: 10 1619
SspI AAT'ATT
Cuts at: 0 169 1629
Size: 169 1460
StuI AGG'CCT
Cuts at: 0 244 1629
Size: 244 1385
StyI C'CwwG_G
Cuts at: 0 245 550 1629
Size: 245 305 1079
Fragments arranged by size:
1079 305 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 923 1629
Size: 923 706
TatI w'GTAC_w
Cuts at: 0 312 1344 1471 1629
Size: 312 1032 127 158
Fragments arranged by size:
1032 312 158 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1237 1269 1629
Size: 1237 32 360
Fragments arranged by size:
1237 360 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1020 1629
Size: 1020 609
Enzymes that do cut:
Acc65I AclI AcuI AleI AlwNI ApoI BanI BanII
BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrGI BssHII BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DraI DraIII EaeI EciI EcoICRI Eco57MI
FspI FspAI HaeII Hin4I HincII HindIII KpnI MfeI
MscI MslI NspI PasI PflMI PsiI PstI SacI
ScaI SfcI SphI SspI StuI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmtI BplI BpuEI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BspEI BsrBI BsrDI
BsrFI BstBI BstEII BstZ17I BtgZI ClaI CspCI DrdI
EagI EarI EcoNI EcoO109I EcoRI EcoRV FalI FseI
HpaI KasI MluI MmeI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI