Restriction map for
pFN21AE5672
(Linear) (Six-base) MAPSORT of: KIEE5672.seq Check: 8284 from: 1 to: 1635
KIEE5672.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1218 1635
Size: 1218 417
AccI GT'mk_AC
Cuts at: 0 482 1286 1522 1635
Size: 482 804 236 113
Fragments arranged by size:
804 482 236 113
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1528 1635
Size: 1027 501 107
Fragments arranged by size:
1027 501 107
AleI CACnn'nnGTG
Cuts at: 0 805 1635
Size: 805 830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 823 1635
Size: 823 812
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 742 774 1635
Size: 742 32 861
Fragments arranged by size:
861 742 32
AlwNI CAG_nnn'CTG
Cuts at: 0 44 1050 1635
Size: 44 1006 585
Fragments arranged by size:
1006 585 44
ApaI G_GGCC'C
Cuts at: 0 265 1635
Size: 265 1370
AscI GG'CGCG_CC
Cuts at: 0 1171 1635
Size: 1171 464
AvaI C'yCGr_G
Cuts at: 0 256 864 943 1054 1635
Size: 256 608 79 111 581
Fragments arranged by size:
608 581 256 111 79
AvrII C'CTAG_G
Cuts at: 0 366 1635
Size: 366 1269
BanI G'GyrC_C
Cuts at: 0 100 728 1635
Size: 100 628 907
Fragments arranged by size:
907 628 100
BanII G_rGCy'C
Cuts at: 0 265 1635
Size: 265 1370
BbeI G_GCGC'C
Cuts at: 0 732 1635
Size: 732 903
BbsI GAAGACnn'nnnn_
Cuts at: 0 176 182 462 1635
Size: 176 6 280 1173
Fragments arranged by size:
1173 280 176 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 588 622 1635
Size: 588 34 1013
Fragments arranged by size:
1013 588 34
BglI GCCn_nnn'nGGC
Cuts at: 0 778 1635
Size: 778 857
BlpI GC'TnA_GC
Cuts at: 0 406 628 1635
Size: 406 222 1007
Fragments arranged by size:
1007 406 222
Bme1580I G_kGCm'C
Cuts at: 0 105 265 1310 1635
Size: 105 160 1045 325
Fragments arranged by size:
1045 325 160 105
BmrI ACTGGGnnnn_n'
Cuts at: 0 570 1635
Size: 570 1065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 565 780 925 1635
Size: 51 514 215 145 710
Fragments arranged by size:
710 514 215 145 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 625 1147 1635
Size: 559 66 522 488
Fragments arranged by size:
559 522 488 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 1390 1635
Size: 1390 245
BsaAI yAC'GTr
Cuts at: 0 912 1635
Size: 912 723
BsaHI Gr'CG_yC
Cuts at: 0 729 1635
Size: 729 906
BsaWI w'CCGG_w
Cuts at: 0 1183 1635
Size: 1183 452
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 181 211 742 772 907 937 1635
Size: 181 30 531 30 135 30 698
Fragments arranged by size:
698 531 181 135 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 33 199 202 442 562 625 694 753
Size: 33 166 3 240 120 63 69 59
Cuts at: 753 862 1635
Size: 109 773
Fragments arranged by size:
773 240 166 120 109 69 63 59
33 3
BseYI C'CCAG_C
Cuts at: 0 265 552 1309 1635
Size: 265 287 757 326
Fragments arranged by size:
757 326 287 265
BsiEI CG_ry'CG
Cuts at: 0 456 467 1635
Size: 456 11 1168
Fragments arranged by size:
1168 456 11
BsiHKAI G_wGCw'C
Cuts at: 0 518 812 911 1301 1635
Size: 518 294 99 390 334
Fragments arranged by size:
518 390 334 294 99
BsmBI CGTCTCn'nnnn_
Cuts at: 0 15 819 1635
Size: 15 804 816
Fragments arranged by size:
816 804 15
Bsp1286I G_dGCh'C
Cuts at: 0 105 265 518 812 911 1301 1310 1635
Size: 105 160 253 294 99 390 9 325
Fragments arranged by size:
390 325 294 253 160 105 99 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 663 899 959 1218 1400 1635
Size: 663 236 60 259 182 235
Fragments arranged by size:
663 259 236 235 182 60
BsrBI CCG'CTC
Cuts at: 0 615 1035 1635
Size: 615 420 600
Fragments arranged by size:
615 600 420
BsrDI GCAATG_nn'
Cuts at: 0 1430 1635
Size: 1430 205
BsrFI r'CCGG_y
Cuts at: 0 346 1635
Size: 346 1289
BssHII G'CGCG_C
Cuts at: 0 660 662 991 1171 1635
Size: 660 2 329 180 464
Fragments arranged by size:
660 464 329 180 2
BssSI C'ACGA_G
Cuts at: 0 652 1635
Size: 652 983
BstAPI GCAn_nnn'nTGC
Cuts at: 0 657 1305 1635
Size: 657 648 330
Fragments arranged by size:
657 648 330
BstEII G'GTnAC_C
Cuts at: 0 240 1635
Size: 240 1395
BstXI CCAn_nnnn'nTGG
Cuts at: 0 789 1635
Size: 789 846
BstYI r'GATC_y
Cuts at: 0 644 1635
Size: 644 991
BstZ17I GTA'TAC
Cuts at: 0 1523 1635
Size: 1523 112
Bsu36I CC'TnA_GG
Cuts at: 0 979 1635
Size: 979 656
BtgI C'CryG_G
Cuts at: 0 129 818 842 1256 1352 1370 1635
Size: 129 689 24 414 96 18 265
Fragments arranged by size:
689 414 265 129 96 24 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1236 1635
Size: 1236 399
DraIII CAC_nnn'GTG
Cuts at: 0 1305 1635
Size: 1305 330
DrdI GACnn_nn'nnGTC
Cuts at: 0 466 1635
Size: 466 1169
EaeI y'GGCC_r
Cuts at: 0 312 344 377 464 770 1022 1086 1635
Size: 312 32 33 87 306 252 64 549
Fragments arranged by size:
549 312 306 252 87 64 33 32
EagI C'GGCC_G
Cuts at: 0 464 1635
Size: 464 1171
EarI CTCTTCn'nnn_
Cuts at: 0 212 700 1252 1635
Size: 212 488 552 383
Fragments arranged by size:
552 488 383 212
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 700 1635
Size: 700 935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 565 780 925 1027 1528 1635
Size: 51 514 215 145 102 501 107
Fragments arranged by size:
514 501 215 145 107 102 51
EcoO109I rG'GnC_Cy
Cuts at: 0 41 261 262 1635
Size: 41 220 1 1373
Fragments arranged by size:
1373 220 41 1
FspI TGC'GCA
Cuts at: 0 1147 1635
Size: 1147 488
FspAI rTGC'GCAy
Cuts at: 0 1147 1635
Size: 1147 488
HaeII r_GCGC'y
Cuts at: 0 732 829 835 987 1001 1635
Size: 732 97 6 152 14 634
Fragments arranged by size:
732 634 152 97 14 6
KasI G'GCGC_C
Cuts at: 0 728 1635
Size: 728 907
MscI TGG'CCA
Cuts at: 0 314 379 1024 1635
Size: 314 65 645 611
Fragments arranged by size:
645 611 314 65
MslI CAynn'nnrTG
Cuts at: 0 787 805 1255 1635
Size: 787 18 450 380
Fragments arranged by size:
787 450 380 18
MspA1I CmG'CkG
Cuts at: 0 327 477 726 1047 1354 1635
Size: 327 150 249 321 307 281
Fragments arranged by size:
327 321 307 281 249 150
NaeI GCC'GGC
Cuts at: 0 348 1635
Size: 348 1287
NarI GG'CG_CC
Cuts at: 0 729 1635
Size: 729 906
NcoI C'CATG_G
Cuts at: 0 1256 1635
Size: 1256 379
NgoMIV G'CCGG_C
Cuts at: 0 346 1635
Size: 346 1289
NspI r_CATG'y
Cuts at: 0 708 1635
Size: 708 927
PasI CC'CwG_GG
Cuts at: 0 96 1635
Size: 96 1539
PflMI CCAn_nnn'nTGG
Cuts at: 0 1095 1230 1635
Size: 1095 135 405
Fragments arranged by size:
1095 405 135
PfoI T'CCnGG_A
Cuts at: 0 28 542 902 1635
Size: 28 514 360 733
Fragments arranged by size:
733 514 360 28
PmlI CAC'GTG
Cuts at: 0 912 1635
Size: 912 723
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 742 774 1635
Size: 742 32 861
Fragments arranged by size:
861 742 32
PspOMI G'GGCC_C
Cuts at: 0 261 1635
Size: 261 1374
PspXI vC'TCGA_Gb
Cuts at: 0 943 1054 1635
Size: 943 111 581
Fragments arranged by size:
943 581 111
PvuII CAG'CTG
Cuts at: 0 327 477 1047 1635
Size: 327 150 570 588
Fragments arranged by size:
588 570 327 150
SacII CC_GC'GG
Cuts at: 0 1355 1635
Size: 1355 280
SapI GCTCTTCn'nnn_
Cuts at: 0 1252 1635
Size: 1252 383
SexAI A'CCwGG_T
Cuts at: 0 1007 1016 1635
Size: 1007 9 619
Fragments arranged by size:
1007 619 9
SfcI C'TryA_G
Cuts at: 0 36 1497 1635
Size: 36 1461 138
Fragments arranged by size:
1461 138 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 778 1635
Size: 778 857
SfoI GGC'GCC
Cuts at: 0 730 1635
Size: 730 905
SmlI C'TyrA_G
Cuts at: 0 574 640 943 1054 1162 1635
Size: 574 66 303 111 108 473
Fragments arranged by size:
574 473 303 111 108 66
StyI C'CwwG_G
Cuts at: 0 366 776 1256 1635
Size: 366 410 480 379
Fragments arranged by size:
480 410 379 366
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 88 298 1635
Size: 88 210 1337
Fragments arranged by size:
1337 210 88
TatI w'GTAC_w
Cuts at: 0 578 1635
Size: 578 1057
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 181 213 907 939 1635
Size: 181 32 694 32 696
Fragments arranged by size:
696 694 181 32 32
XhoI C'TCGA_G
Cuts at: 0 943 1054 1635
Size: 943 111 581
Fragments arranged by size:
943 581 111
XmnI GAAnn'nnTTC
Cuts at: 0 757 1635
Size: 757 878
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AloI AlwNI ApaI
AscI AvaI AvrII BanI BanII BbeI BbsI BcgI
BglI BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I FspI
FspAI HaeII KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpiI
PspOMI PspXI PvuII SacII SapI SexAI SfcI SfiI
SfoI SmlI StyI TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
ApaLI ApoI AseI AsiSI BaeI BamHI BbvCI BciVI
BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BsaBI
BsgI BsiWI BsmI BspEI BspHI BsrGI BstBI BtsI
ClaI CspCI DraI EcoICRI EcoNI EcoRI EcoRV FalI
FseI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PpuMI PshAI PsiI PsrI PstI PvuI RsrII
SacI SalI SanDI SbfI ScaI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XmaI ZraI