Restriction map for
pFN21AE5668
(Linear) (Six-base) MAPSORT of: KIEE5668.seq Check: 1982 from: 1 to: 1584
KIEE5668.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 874 1584
Size: 874 710
AatII G_ACGT'C
Cuts at: 0 718 1472 1584
Size: 718 754 112
Fragments arranged by size:
754 718 112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 142 563 640 1584
Size: 28 114 421 77 944
Fragments arranged by size:
944 421 114 77 28
AfeI AGC'GCT
Cuts at: 0 540 1584
Size: 540 1044
AflIII A'CryG_T
Cuts at: 0 584 1584
Size: 584 1000
AhdI GACnn_n'nnGTC
Cuts at: 0 714 1584
Size: 714 870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 754 1584
Size: 754 830
AlwNI CAG_nnn'CTG
Cuts at: 0 1042 1584
Size: 1042 542
ApaI G_GGCC'C
Cuts at: 0 141 1584
Size: 141 1443
ApaLI G'TGCA_C
Cuts at: 0 180 982 1584
Size: 180 802 602
Fragments arranged by size:
802 602 180
AvaI C'yCGr_G
Cuts at: 0 594 1556 1584
Size: 594 962 28
Fragments arranged by size:
962 594 28
BanI G'GyrC_C
Cuts at: 0 862 1584
Size: 862 722
BanII G_rGCy'C
Cuts at: 0 141 157 1292 1358 1584
Size: 141 16 1135 66 226
Fragments arranged by size:
1135 226 141 66 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 551 552 628 1584
Size: 551 1 76 956
Fragments arranged by size:
956 551 76 1
BbvCI CC'TCA_GC
Cuts at: 0 1155 1456 1584
Size: 1155 301 128
Fragments arranged by size:
1155 301 128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 223 257 475 509 1391 1425 1584
Size: 223 34 218 34 882 34 159
Fragments arranged by size:
882 223 218 159 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1502 1584
Size: 1502 82
Bme1580I G_kGCm'C
Cuts at: 0 132 141 184 488 865 986 1584
Size: 132 9 43 304 377 121 598
Fragments arranged by size:
598 377 304 132 121 43 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 998 1372 1584
Size: 424 574 374 212
Fragments arranged by size:
574 424 374 212
Bpu10I CC'TnA_GC
Cuts at: 0 1155 1456 1584
Size: 1155 301 128
Fragments arranged by size:
1155 301 128
BsaI GGTCTCn'nnnn_
Cuts at: 0 1246 1584
Size: 1246 338
BsaHI Gr'CG_yC
Cuts at: 0 35 715 1469 1584
Size: 35 680 754 115
Fragments arranged by size:
754 680 115 35
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 1584
Size: 85 30 1469
Fragments arranged by size:
1469 85 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 34 37 1584
Size: 31 3 3 1547
Fragments arranged by size:
1547 31 3 3
BseYI C'CCAG_C
Cuts at: 0 331 481 780 1144 1584
Size: 331 150 299 364 440
Fragments arranged by size:
440 364 331 299 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 214 760 1055 1584
Size: 64 150 546 295 529
Fragments arranged by size:
546 529 295 150 64
BsiEI CG_ry'CG
Cuts at: 0 477 613 1584
Size: 477 136 971
Fragments arranged by size:
971 477 136
BsiHKAI G_wGCw'C
Cuts at: 0 184 986 1358 1584
Size: 184 802 372 226
Fragments arranged by size:
802 372 226 184
BsmBI CGTCTCn'nnnn_
Cuts at: 0 706 937 1584
Size: 706 231 647
Fragments arranged by size:
706 647 231
Bsp1286I G_dGCh'C
Cuts at: 0 132 141 157 184 488 865 986 1292
Size: 132 9 16 27 304 377 121 306
Cuts at: 1292 1358 1584
Size: 66 226
Fragments arranged by size:
377 306 304 226 132 121 66 27
16 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 695 854 874 1584
Size: 695 159 20 710
Fragments arranged by size:
710 695 159 20
BsrBI CCG'CTC
Cuts at: 0 111 1584
Size: 111 1473
BsrDI GCAATG_nn'
Cuts at: 0 651 1584
Size: 651 933
BsrFI r'CCGG_y
Cuts at: 0 42 1584
Size: 42 1542
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1042 1584
Size: 1042 542
BstBI TT'CG_AA
Cuts at: 0 722 1584
Size: 722 862
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1167 1584
Size: 1167 417
BstYI r'GATC_y
Cuts at: 0 743 1038 1139 1584
Size: 743 295 101 445
Fragments arranged by size:
743 445 295 101
BtgI C'CryG_G
Cuts at: 0 613 881 1130 1584
Size: 613 268 249 454
Fragments arranged by size:
613 454 268 249
BtsI GCAGTG_nn'
Cuts at: 0 76 920 1440 1584
Size: 76 844 520 144
Fragments arranged by size:
844 520 144 76
DraIII CAC_nnn'GTG
Cuts at: 0 275 1584
Size: 275 1309
EaeI y'GGCC_r
Cuts at: 0 610 1169 1584
Size: 610 559 415
Fragments arranged by size:
610 559 415
EagI C'GGCC_G
Cuts at: 0 610 1584
Size: 610 974
EcoICRI GAG'CTC
Cuts at: 0 1356 1584
Size: 1356 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 142 424 563 640 998 1372 1584
Size: 28 114 282 139 77 358 374 212
Fragments arranged by size:
374 358 282 212 139 114 77 28
EcoO109I rG'GnC_Cy
Cuts at: 0 138 1584
Size: 138 1446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 576 1584
Size: 576 1008
HaeII r_GCGC'y
Cuts at: 0 542 1584
Size: 542 1042
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 100 132 700 732 735 767 1584
Size: 100 32 568 32 3 32 817
Fragments arranged by size:
817 568 100 32 32 32 3
MscI TGG'CCA
Cuts at: 0 1171 1584
Size: 1171 413
MslI CAynn'nnrTG
Cuts at: 0 382 736 1216 1584
Size: 382 354 480 368
Fragments arranged by size:
480 382 368 354
MspA1I CmG'CkG
Cuts at: 0 402 615 780 834 1584
Size: 402 213 165 54 750
Fragments arranged by size:
750 402 213 165 54
NcoI C'CATG_G
Cuts at: 0 881 1130 1584
Size: 881 249 454
Fragments arranged by size:
881 454 249
NotI GC'GGCC_GC
Cuts at: 0 610 1584
Size: 610 974
NspI r_CATG'y
Cuts at: 0 462 504 990 1584
Size: 462 42 486 594
Fragments arranged by size:
594 486 462 42
PasI CC'CwG_GG
Cuts at: 0 857 1584
Size: 857 727
PspOMI G'GGCC_C
Cuts at: 0 137 1584
Size: 137 1447
PstI C_TGCA'G
Cuts at: 0 416 538 1584
Size: 416 122 1046
Fragments arranged by size:
1046 416 122
PvuII CAG'CTG
Cuts at: 0 402 780 1584
Size: 402 378 804
Fragments arranged by size:
804 402 378
SacI G_AGCT'C
Cuts at: 0 1358 1584
Size: 1358 226
SacII CC_GC'GG
Cuts at: 0 616 1584
Size: 616 968
ScaI AGT'ACT
Cuts at: 0 199 1078 1584
Size: 199 879 506
Fragments arranged by size:
879 506 199
SexAI A'CCwGG_T
Cuts at: 0 953 1584
Size: 953 631
SfcI C'TryA_G
Cuts at: 0 412 534 912 1584
Size: 412 122 378 672
Fragments arranged by size:
672 412 378 122
StuI AGG'CCT
Cuts at: 0 58 1273 1584
Size: 58 1215 311
Fragments arranged by size:
1215 311 58
StyI C'CwwG_G
Cuts at: 0 881 1130 1584
Size: 881 249 454
Fragments arranged by size:
881 454 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 491 1285 1584
Size: 19 472 794 299
Fragments arranged by size:
794 472 299 19
TatI w'GTAC_w
Cuts at: 0 197 1009 1022 1076 1584
Size: 197 812 13 54 508
Fragments arranged by size:
812 508 197 54 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 85 117 1584
Size: 85 32 1467
Fragments arranged by size:
1467 85 32
Tth111I GACn'n_nGTC
Cuts at: 0 1468 1584
Size: 1468 116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 784 1584
Size: 784 800
ZraI GAC'GTC
Cuts at: 0 716 1470 1584
Size: 716 754 114
Fragments arranged by size:
754 716 114
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AlfI AlwNI
ApaI ApaLI AvaI BanI BanII BbsI BbvCI BcgI
BglI Bme1580I BpmI Bpu10I BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstAPI BstBI BstXI BstYI BtgI BtsI
DraIII EaeI EagI EcoICRI Eco57MI EcoO109I FalI HaeII
Hin4I MscI MslI MspA1I NcoI NotI NspI PasI
PspOMI PstI PvuII SacI SacII ScaI SexAI SfcI
StuI StyI TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AleI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI
BsrGI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EarI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NruI NsiI PacI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XhoI XmaI XmnI