Restriction map for
pFN21AE5666
(Linear) (Six-base) MAPSORT of: KIEE5666.seq Check: 1137 from: 1 to: 1560
KIEE5666.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 1019 1560
Size: 279 740 541
Fragments arranged by size:
740 541 279
AflIII A'CryG_T
Cuts at: 0 977 1560
Size: 977 583
AleI CACnn'nnGTG
Cuts at: 0 862 1560
Size: 862 698
AlwNI CAG_nnn'CTG
Cuts at: 0 1405 1560
Size: 1405 155
ApoI r'AATT_y
Cuts at: 0 625 1560
Size: 625 935
AvaI C'yCGr_G
Cuts at: 0 104 1340 1560
Size: 104 1236 220
Fragments arranged by size:
1236 220 104
BamHI G'GATC_C
Cuts at: 0 172 1214 1560
Size: 172 1042 346
Fragments arranged by size:
1042 346 172
BanI G'GyrC_C
Cuts at: 0 1273 1560
Size: 1273 287
BclI T'GATC_A
Cuts at: 0 854 1560
Size: 854 706
BmgBI CAC'GTC
Cuts at: 0 18 1060 1560
Size: 18 1042 500
Fragments arranged by size:
1042 500 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1194 1560
Size: 1194 366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 936 950 1153 1314 1560
Size: 712 224 14 203 161 246
Fragments arranged by size:
712 246 224 203 161 14
Bpu10I CC'TnA_GC
Cuts at: 0 1477 1560
Size: 1477 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 1560
Size: 225 1335
BsaI GGTCTCn'nnnn_
Cuts at: 0 226 520 543 671 736 1560
Size: 226 294 23 128 65 824
Fragments arranged by size:
824 294 226 128 65 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 668 698 1560
Size: 668 30 862
Fragments arranged by size:
862 668 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1183 1560
Size: 1183 377
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1403 1560
Size: 1403 157
BsrBI CCG'CTC
Cuts at: 0 657 1298 1560
Size: 657 641 262
Fragments arranged by size:
657 641 262
BssSI C'ACGA_G
Cuts at: 0 860 1560
Size: 860 700
BstAPI GCAn_nnn'nTGC
Cuts at: 0 11 1560
Size: 11 1549
BstXI CCAn_nnnn'nTGG
Cuts at: 0 317 1053 1113 1443 1560
Size: 317 736 60 330 117
Fragments arranged by size:
736 330 317 117 60
BstYI r'GATC_y
Cuts at: 0 172 637 1214 1346 1560
Size: 172 465 577 132 214
Fragments arranged by size:
577 465 214 172 132
BtgI C'CryG_G
Cuts at: 0 400 1358 1560
Size: 400 958 202
Fragments arranged by size:
958 400 202
BtsI GCAGTG_nn'
Cuts at: 0 518 577 1560
Size: 518 59 983
Fragments arranged by size:
983 518 59
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 483 518 1560
Size: 483 35 1042
Fragments arranged by size:
1042 483 35
EarI CTCTTCn'nnn_
Cuts at: 0 416 1560
Size: 416 1144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 650 1560
Size: 650 910
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 712 936 950 1019 1153 1314 1560
Size: 279 433 224 14 69 134 161 246
Fragments arranged by size:
433 279 246 224 161 134 69 14
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 834 1560
Size: 834 726
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 117 149 716 748 843 875 1206 1238
Size: 117 32 567 32 95 32 331 32
Cuts at: 1238 1560
Size: 322
Fragments arranged by size:
567 331 322 117 95 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 962 1560
Size: 162 800 598
Fragments arranged by size:
800 598 162
MslI CAynn'nnrTG
Cuts at: 0 315 439 862 1560
Size: 315 124 423 698
Fragments arranged by size:
698 423 315 124
NcoI C'CATG_G
Cuts at: 0 400 1358 1560
Size: 400 958 202
Fragments arranged by size:
958 400 202
NspI r_CATG'y
Cuts at: 0 1357 1560
Size: 1357 203
PasI CC'CwG_GG
Cuts at: 0 1210 1560
Size: 1210 350
PflMI CCAn_nnn'nTGG
Cuts at: 0 1251 1292 1560
Size: 1251 41 268
Fragments arranged by size:
1251 268 41
PsiI TTA'TAA
Cuts at: 0 902 1560
Size: 902 658
PstI C_TGCA'G
Cuts at: 0 525 1560
Size: 525 1035
ScaI AGT'ACT
Cuts at: 0 1177 1560
Size: 1177 383
SfcI C'TryA_G
Cuts at: 0 521 1316 1560
Size: 521 795 244
Fragments arranged by size:
795 521 244
SmaI CCC'GGG
Cuts at: 0 106 1560
Size: 106 1454
SmlI C'TyrA_G
Cuts at: 0 204 1560
Size: 204 1356
SphI G_CATG'C
Cuts at: 0 1357 1560
Size: 1357 203
StuI AGG'CCT
Cuts at: 0 699 1472 1560
Size: 699 773 88
Fragments arranged by size:
773 699 88
StyI C'CwwG_G
Cuts at: 0 165 215 400 672 1106 1358 1560
Size: 165 50 185 272 434 252 202
Fragments arranged by size:
434 272 252 202 185 165 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 695 1560
Size: 695 865
TatI w'GTAC_w
Cuts at: 0 1175 1560
Size: 1175 385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 71 103 668 700 1560
Size: 71 32 565 32 860
Fragments arranged by size:
860 565 71 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 19 1560
Size: 19 1541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1440 1560
Size: 1440 120
XmaI C'CCGG_G
Cuts at: 0 104 1560
Size: 104 1456
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApoI AvaI BamHI BanI
BclI BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaXI
BseRI BsgI BsrBI BssSI BstAPI BstXI BstYI BtgI
BtsI CspCI EarI EciI Eco57MI FalI Hin4I MmeI
MslI NcoI NspI PasI PflMI PsiI PstI ScaI
SfcI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EaeI EagI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MscI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI XbaI XhoI XmnI ZraI