(Linear) (Six-base) MAPSORT of: KIEE5664.seq  Check: 7303  from: 1  to: 1572



KIEE5664.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:05  ..

AleI CACnn'nnGTG

Cuts at:      0    1107    1572 
   Size:       1107     465
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      44    1125    1572 
   Size:         44    1081     447

  Fragments arranged by size: 

                1081     447      44
 
AlwNI CAG_nnn'CTG

Cuts at:      0     156    1572 
   Size:        156    1416
 
ApoI r'AATT_y

Cuts at:      0     266     402     739    1572 
   Size:        266     136     337     833

  Fragments arranged by size: 

                 833     337     266     136
 
AseI AT'TA_AT

Cuts at:      0     884    1572 
   Size:        884     688
 
AvaI C'yCGr_G

Cuts at:      0    1088    1572 
   Size:       1088     484
 
AvrII C'CTAG_G

Cuts at:      0    1047    1572 
   Size:       1047     525
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1478    1572 
   Size:       1478      94
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1112    1497    1572 
   Size:       1112     385      75

  Fragments arranged by size: 

                1112     385      75
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1280    1572 
   Size:       1280     292
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     166    1572 
   Size:        166    1406
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93    1572 
   Size:         93    1479
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231    1572 
   Size:        231    1341
 
BsaBI GATnn'nnATC

Cuts at:      0     953    1572 
   Size:        953     619
 
BsaWI w'CCGG_w

Cuts at:      0     435    1572 
   Size:        435    1137
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      66      96    1129    1159    1572 
   Size:         66      30    1033      30     413

  Fragments arranged by size: 

                1033     413      66      30      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1241    1572 
   Size:       1241     331
 
BsmI GAATG_Cn'

Cuts at:      0     820    1572 
   Size:        820     752
 
BstYI r'GATC_y

Cuts at:      0     836    1572 
   Size:        836     736
 
Bsu36I CC'TnA_GG

Cuts at:      0    1361    1572 
   Size:       1361     211
 
BtgI C'CryG_G

Cuts at:      0     418    1572 
   Size:        418    1154
 
DraI TTT'AAA

Cuts at:      0     744     935    1572 
   Size:        744     191     637

  Fragments arranged by size: 

                 744     637     191
 
EaeI y'GGCC_r

Cuts at:      0    1281    1572 
   Size:       1281     291
 
EarI CTCTTCn'nnn_

Cuts at:      0    1514    1572 
   Size:       1514      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93    1572 
   Size:         93    1479
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     398     814    1572 
   Size:        398     416     758

  Fragments arranged by size: 

                 758     416     398
 
HincII GTy'rAC

Cuts at:      0     129    1572 
   Size:        129    1443
 
HpaI GTT'AAC

Cuts at:      0     129    1572 
   Size:        129    1443
 
MfeI C'AATT_G

Cuts at:      0     849    1572 
   Size:        849     723
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1019    1572 
   Size:       1019     553
 
MscI TGG'CCA

Cuts at:      0    1283    1572 
   Size:       1283     289
 
MslI CAynn'nnrTG

Cuts at:      0    1107    1572 
   Size:       1107     465
 
MspA1I CmG'CkG

Cuts at:      0     321    1572 
   Size:        321    1251
 
NspI r_CATG'y

Cuts at:      0     994    1009    1572 
   Size:        994      15     563

  Fragments arranged by size: 

                 994     563      15
 
PstI C_TGCA'G

Cuts at:      0    1014    1572 
   Size:       1014     558
 
PvuII CAG'CTG

Cuts at:      0     321    1572 
   Size:        321    1251
 
ScaI AGT'ACT

Cuts at:      0     216     998    1342    1572 
   Size:        216     782     344     230

  Fragments arranged by size: 

                 782     344     230     216
 
SfcI C'TryA_G

Cuts at:      0    1010    1572 
   Size:       1010     562
 
SmlI C'TyrA_G

Cuts at:      0     246    1088    1572 
   Size:        246     842     484

  Fragments arranged by size: 

                 842     484     246
 
SphI G_CATG'C

Cuts at:      0    1009    1572 
   Size:       1009     563
 
StuI AGG'CCT

Cuts at:      0     242    1572 
   Size:        242    1330
 
StyI C'CwwG_G

Cuts at:      0     588    1047    1572 
   Size:        588     459     525

  Fragments arranged by size: 

                 588     525     459
 
TatI w'GTAC_w

Cuts at:      0     120     214     289     468     996    1340    1572 
   Size:        120      94      75     179     528     344     232

  Fragments arranged by size: 

                 528     344     232     179     120      94      75
 
XhoI C'TCGA_G

Cuts at:      0    1088    1572 
   Size:       1088     484
 
XmnI GAAnn'nnTTC

Cuts at:      0     583    1572 
   Size:        583     989
 

 Enzymes that do cut:

     AleI     AlfI    AlwNI     ApoI     AseI     AvaI    AvrII     BbsI
    BciVI     BglI     BmrI     BpmI    BpuEI    BsaBI    BsaWI    BsaXI
     BsgI     BsmI    BstYI   Bsu36I     BtgI     DraI     EaeI     EarI
  Eco57MI     FalI   HincII     HpaI     MfeI     MmeI     MscI     MslI
   MspA1I     NspI     PstI    PvuII     ScaI     SfcI     SmlI     SphI
     StuI     StyI     TatI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AloI     ApaI    ApaLI     AscI    AsiSI
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI     BclI
    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaHI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     KasI     KpnI     MluI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI