Restriction map for
pFN21AE5663
(Linear) (Six-base) MAPSORT of: KIEE5663.seq Check: 6824 from: 1 to: 1557
KIEE5663.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AatII G_ACGT'C
Cuts at: 0 48 1557
Size: 48 1509
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1557
Size: 253 1304
AflIII A'CryG_T
Cuts at: 0 1136 1557
Size: 1136 421
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 399 951 1557
Size: 399 552 606
Fragments arranged by size:
606 552 399
ApoI r'AATT_y
Cuts at: 0 171 788 1408 1557
Size: 171 617 620 149
Fragments arranged by size:
620 617 171 149
AseI AT'TA_AT
Cuts at: 0 977 1557
Size: 977 580
BamHI G'GATC_C
Cuts at: 0 426 1557
Size: 426 1131
BanI G'GyrC_C
Cuts at: 0 1180 1207 1557
Size: 1180 27 350
Fragments arranged by size:
1180 350 27
BanII G_rGCy'C
Cuts at: 0 410 773 1557
Size: 410 363 784
Fragments arranged by size:
784 410 363
BbsI GAAGACnn'nnnn_
Cuts at: 0 250 771 1072 1557
Size: 250 521 301 485
Fragments arranged by size:
521 485 301 250
BbvCI CC'TCA_GC
Cuts at: 0 1094 1557
Size: 1094 463
BglII A'GATC_T
Cuts at: 0 1369 1557
Size: 1369 188
BlpI GC'TnA_GC
Cuts at: 0 939 1557
Size: 939 618
Bme1580I G_kGCm'C
Cuts at: 0 1434 1557
Size: 1434 123
BmrI ACTGGGnnnn_n'
Cuts at: 0 769 1223 1508 1557
Size: 769 454 285 49
Fragments arranged by size:
769 454 285 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1313 1557
Size: 1313 244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 1308 1557
Size: 424 884 249
Fragments arranged by size:
884 424 249
Bpu10I CC'TnA_GC
Cuts at: 0 443 1094 1557
Size: 443 651 463
Fragments arranged by size:
651 463 443
BsaI GGTCTCn'nnnn_
Cuts at: 0 478 1557
Size: 478 1079
BsaHI Gr'CG_yC
Cuts at: 0 45 613 631 1557
Size: 45 568 18 926
Fragments arranged by size:
926 568 45 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 60 90 397 427 1557
Size: 60 30 307 30 1130
Fragments arranged by size:
1130 307 60 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 935 1557
Size: 935 622
BseYI C'CCAG_C
Cuts at: 0 280 1015 1557
Size: 280 735 542
Fragments arranged by size:
735 542 280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 405 1557
Size: 182 223 1152
Fragments arranged by size:
1152 223 182
BsiHKAI G_wGCw'C
Cuts at: 0 410 1557
Size: 410 1147
BsmI GAATG_Cn'
Cuts at: 0 1396 1557
Size: 1396 161
BsmBI CGTCTCn'nnnn_
Cuts at: 0 51 431 604 1557
Size: 51 380 173 953
Fragments arranged by size:
953 380 173 51
Bsp1286I G_dGCh'C
Cuts at: 0 410 773 1434 1557
Size: 410 363 661 123
Fragments arranged by size:
661 410 363 123
BspHI T'CATG_A
Cuts at: 0 902 1557
Size: 902 655
BsrBI CCG'CTC
Cuts at: 0 463 1557
Size: 463 1094
BsrDI GCAATG_nn'
Cuts at: 0 737 1557
Size: 737 820
BsrGI T'GTAC_A
Cuts at: 0 1384 1557
Size: 1384 173
BstBI TT'CG_AA
Cuts at: 0 1304 1557
Size: 1304 253
BstYI r'GATC_y
Cuts at: 0 426 760 1369 1557
Size: 426 334 609 188
Fragments arranged by size:
609 426 334 188
BtgI C'CryG_G
Cuts at: 0 1184 1557
Size: 1184 373
EaeI y'GGCC_r
Cuts at: 0 50 752 1557
Size: 50 702 805
Fragments arranged by size:
805 702 50
EarI CTCTTCn'nnn_
Cuts at: 0 20 1258 1306 1557
Size: 20 1238 48 251
Fragments arranged by size:
1238 251 48 20
EcoICRI GAG'CTC
Cuts at: 0 408 1557
Size: 408 1149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 424 1308 1557
Size: 253 171 884 249
Fragments arranged by size:
884 253 249 171
EcoO109I rG'GnC_Cy
Cuts at: 0 102 1557
Size: 102 1455
EcoRI G'AATT_C
Cuts at: 0 1408 1557
Size: 1408 149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 23 55 60 92 397 429 728 760
Size: 23 32 5 32 305 32 299 32
Cuts at: 760 1557
Size: 797
Fragments arranged by size:
797 305 299 32 32 32 32 23
5
HincII GTy'rAC
Cuts at: 0 1350 1557
Size: 1350 207
HindIII A'AGCT_T
Cuts at: 0 961 1557
Size: 961 596
HpaI GTT'AAC
Cuts at: 0 1350 1557
Size: 1350 207
MfeI C'AATT_G
Cuts at: 0 601 1557
Size: 601 956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 1557
Size: 66 1491
MscI TGG'CCA
Cuts at: 0 754 1557
Size: 754 803
MspA1I CmG'CkG
Cuts at: 0 316 379 1019 1390 1557
Size: 316 63 640 371 167
Fragments arranged by size:
640 371 316 167 63
NcoI C'CATG_G
Cuts at: 0 1184 1557
Size: 1184 373
NspI r_CATG'y
Cuts at: 0 1385 1557
Size: 1385 172
PflMI CCAn_nnn'nTGG
Cuts at: 0 606 713 1557
Size: 606 107 844
Fragments arranged by size:
844 606 107
PshAI GACnn'nnGTC
Cuts at: 0 708 1557
Size: 708 849
PstI C_TGCA'G
Cuts at: 0 1377 1395 1557
Size: 1377 18 162
Fragments arranged by size:
1377 162 18
PvuII CAG'CTG
Cuts at: 0 316 1390 1557
Size: 316 1074 167
Fragments arranged by size:
1074 316 167
SacI G_AGCT'C
Cuts at: 0 410 1557
Size: 410 1147
SfcI C'TryA_G
Cuts at: 0 1373 1391 1557
Size: 1373 18 166
Fragments arranged by size:
1373 166 18
SspI AAT'ATT
Cuts at: 0 981 1557
Size: 981 576
StuI AGG'CCT
Cuts at: 0 553 1557
Size: 553 1004
StyI C'CwwG_G
Cuts at: 0 1184 1557
Size: 1184 373
TatI w'GTAC_w
Cuts at: 0 1384 1557
Size: 1384 173
Tth111I GACn'n_nGTC
Cuts at: 0 1282 1557
Size: 1282 275
XmnI GAAnn'nnTTC
Cuts at: 0 120 1408 1557
Size: 120 1288 149
Fragments arranged by size:
1288 149 120
ZraI GAC'GTC
Cuts at: 0 46 1557
Size: 46 1511
Enzymes that do cut:
AatII AcuI AflIII AlfI ApoI AseI BamHI BanI
BanII BbsI BbvCI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrGI
BstBI BstYI BtgI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI Hin4I HincII HindIII HpaI MfeI MmeI MscI
MspA1I NcoI NspI PflMI PshAI PstI PvuII SacI
SfcI SspI StuI StyI TatI Tth111I XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglI
BmgBI BmtI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI
BspEI BspMI BsrFI BssHII BssSI BstAPI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRV FalI FseI FspI
FspAI HaeII KasI KpnI MluI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SwaI TaqII TstI
XbaI XcmI XhoI XmaI