Restriction map for
pFN21AE5662
(Linear) (Six-base) MAPSORT of: KIEE5662.seq Check: 7351 from: 1 to: 1584
KIEE5662.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 361 693 1584
Size: 361 332 891
Fragments arranged by size:
891 361 332
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 925 1171 1523 1584
Size: 499 426 246 352 61
Fragments arranged by size:
499 426 352 246 61
AfeI AGC'GCT
Cuts at: 0 739 1584
Size: 739 845
AflIII A'CryG_T
Cuts at: 0 910 1584
Size: 910 674
AhdI GACnn_n'nnGTC
Cuts at: 0 1499 1584
Size: 1499 85
AleI CACnn'nnGTG
Cuts at: 0 1132 1584
Size: 1132 452
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 247 279 1584
Size: 247 32 1305
Fragments arranged by size:
1305 247 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1017 1371 1509 1584
Size: 1017 354 138 75
Fragments arranged by size:
1017 354 138 75
ApaI G_GGCC'C
Cuts at: 0 322 338 1185 1310 1584
Size: 322 16 847 125 274
Fragments arranged by size:
847 322 274 125 16
AvaI C'yCGr_G
Cuts at: 0 895 1057 1584
Size: 895 162 527
Fragments arranged by size:
895 527 162
BanI G'GyrC_C
Cuts at: 0 621 873 1584
Size: 621 252 711
Fragments arranged by size:
711 621 252
BanII G_rGCy'C
Cuts at: 0 322 338 989 1107 1185 1310 1583 1584
Size: 322 16 651 118 78 125 273 1
Fragments arranged by size:
651 322 273 125 118 78 16 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 304 457 913 1584
Size: 304 153 456 671
Fragments arranged by size:
671 456 304 153
BbvCI CC'TCA_GC
Cuts at: 0 710 1584
Size: 710 874
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 847 881 1584
Size: 847 34 703
Fragments arranged by size:
847 703 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1004 1584
Size: 1004 580
Bme1580I G_kGCm'C
Cuts at: 0 19 322 338 878 894 1185 1310 1584
Size: 19 303 16 540 16 291 125 274
Fragments arranged by size:
540 303 291 274 125 19 16 16
BmgBI CAC'GTC
Cuts at: 0 1496 1584
Size: 1496 88
BmrI ACTGGGnnnn_n'
Cuts at: 0 1526 1584
Size: 1526 58
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 1584
Size: 103 1481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 157 274 406 748 1177 1457 1551
Size: 52 105 117 132 342 429 280 94
Cuts at: 1551 1584
Size: 33
Fragments arranged by size:
429 342 280 132 117 105 94 52
33
Bpu10I CC'TnA_GC
Cuts at: 0 493 710 1584
Size: 493 217 874
Fragments arranged by size:
874 493 217
BsaAI yAC'GTr
Cuts at: 0 652 913 1584
Size: 652 261 671
Fragments arranged by size:
671 652 261
BsaWI w'CCGG_w
Cuts at: 0 293 1203 1584
Size: 293 910 381
Fragments arranged by size:
910 381 293
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 55 247 277 1584
Size: 25 30 192 30 1307
Fragments arranged by size:
1307 192 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 207 1584
Size: 25 182 1377
Fragments arranged by size:
1377 182 25
BseYI C'CCAG_C
Cuts at: 0 321 393 1100 1163 1424 1485 1584
Size: 321 72 707 63 261 61 99
Fragments arranged by size:
707 321 261 99 72 63 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 721 1198 1584
Size: 70 651 477 386
Fragments arranged by size:
651 477 386 70
BsiHKAI G_wGCw'C
Cuts at: 0 289 590 828 1584
Size: 289 301 238 756
Fragments arranged by size:
756 301 289 238
BsmBI CGTCTCn'nnnn_
Cuts at: 0 74 1486 1584
Size: 74 1412 98
Fragments arranged by size:
1412 98 74
Bsp1286I G_dGCh'C
Cuts at: 0 19 289 322 338 590 828 878 894
Size: 19 270 33 16 252 238 50 16
Cuts at: 894 989 1107 1185 1310 1583 1584
Size: 95 118 78 125 273 1
Fragments arranged by size:
273 270 252 238 125 118 95 78
50 33 19 16 16 1
BspEI T'CCGG_A
Cuts at: 0 293 1584
Size: 293 1291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 361 383 693 1584
Size: 361 22 310 891
Fragments arranged by size:
891 361 310 22
BsrBI CCG'CTC
Cuts at: 0 1044 1584
Size: 1044 540
BsrFI r'CCGG_y
Cuts at: 0 644 1339 1584
Size: 644 695 245
Fragments arranged by size:
695 644 245
BstEII G'GTnAC_C
Cuts at: 0 1326 1584
Size: 1326 258
BstXI CCAn_nnnn'nTGG
Cuts at: 0 606 1134 1584
Size: 606 528 450
Fragments arranged by size:
606 528 450
BstYI r'GATC_y
Cuts at: 0 1013 1584
Size: 1013 571
Bsu36I CC'TnA_GG
Cuts at: 0 444 1584
Size: 444 1140
BtgI C'CryG_G
Cuts at: 0 509 1334 1584
Size: 509 825 250
Fragments arranged by size:
825 509 250
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 850 1584
Size: 850 734
BtsI GCAGTG_nn'
Cuts at: 0 1419 1584
Size: 1419 165
DrdI GACnn_nn'nnGTC
Cuts at: 0 91 850 1584
Size: 91 759 734
Fragments arranged by size:
759 734 91
EaeI y'GGCC_r
Cuts at: 0 162 978 1278 1337 1584
Size: 162 816 300 59 247
Fragments arranged by size:
816 300 247 162 59
EarI CTCTTCn'nnn_
Cuts at: 0 184 1584
Size: 184 1400
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 559 1584
Size: 559 1025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 157 274 406 499 748 925 1171
Size: 52 105 117 132 93 249 177 246
Cuts at: 1171 1177 1457 1523 1551 1584
Size: 6 280 66 28 33
Fragments arranged by size:
280 249 246 177 132 117 105 93
66 52 33 28 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1156 1584
Size: 1156 428
EcoO109I rG'GnC_Cy
Cuts at: 0 132 318 334 335 372 1182 1306 1307
Size: 132 186 16 1 37 810 124 1
Cuts at: 1307 1584
Size: 277
Fragments arranged by size:
810 277 186 132 124 37 16 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 894 1584
Size: 894 690
FseI GG_CCGG'CC
Cuts at: 0 1343 1584
Size: 1343 241
FspI TGC'GCA
Cuts at: 0 421 1584
Size: 421 1163
HaeII r_GCGC'y
Cuts at: 0 741 1584
Size: 741 843
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 427 459 1485 1517 1584
Size: 25 32 370 32 1026 32 67
Fragments arranged by size:
1026 370 67 32 32 32 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 1584
Size: 620 964
MscI TGG'CCA
Cuts at: 0 164 1280 1584
Size: 164 1116 304
Fragments arranged by size:
1116 304 164
MslI CAynn'nnrTG
Cuts at: 0 604 940 1132 1174 1222 1584
Size: 604 336 192 42 48 362
Fragments arranged by size:
604 362 336 192 48 42
MspA1I CmG'CkG
Cuts at: 0 54 168 1489 1584
Size: 54 114 1321 95
Fragments arranged by size:
1321 114 95 54
NaeI GCC'GGC
Cuts at: 0 1341 1584
Size: 1341 243
NcoI C'CATG_G
Cuts at: 0 509 1584
Size: 509 1075
NgoMIV G'CCGG_C
Cuts at: 0 1339 1584
Size: 1339 245
NspI r_CATG'y
Cuts at: 0 1179 1584
Size: 1179 405
PasI CC'CwG_GG
Cuts at: 0 313 1584
Size: 313 1271
PfoI T'CCnGG_A
Cuts at: 0 383 524 1433 1473 1567 1584
Size: 383 141 909 40 94 17
Fragments arranged by size:
909 383 141 94 40 17
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 1584
Size: 247 32 1305
Fragments arranged by size:
1305 247 32
PpuMI rG'GwC_Cy
Cuts at: 0 132 372 1584
Size: 132 240 1212
Fragments arranged by size:
1212 240 132
PshAI GACnn'nnGTC
Cuts at: 0 1451 1584
Size: 1451 133
PspOMI G'GGCC_C
Cuts at: 0 318 334 1181 1306 1584
Size: 318 16 847 125 278
Fragments arranged by size:
847 318 278 125 16
PstI C_TGCA'G
Cuts at: 0 344 1376 1584
Size: 344 1032 208
Fragments arranged by size:
1032 344 208
PvuII CAG'CTG
Cuts at: 0 54 1489 1584
Size: 54 1435 95
Fragments arranged by size:
1435 95 54
ScaI AGT'ACT
Cuts at: 0 64 1584
Size: 64 1520
SexAI A'CCwGG_T
Cuts at: 0 791 1584
Size: 791 793
SfcI C'TryA_G
Cuts at: 0 340 1372 1584
Size: 340 1032 212
Fragments arranged by size:
1032 340 212
SnaBI TAC'GTA
Cuts at: 0 652 1584
Size: 652 932
StuI AGG'CCT
Cuts at: 0 202 1531 1584
Size: 202 1329 53
Fragments arranged by size:
1329 202 53
StyI C'CwwG_G
Cuts at: 0 509 1584
Size: 509 1075
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 385 1584
Size: 385 1199
TatI w'GTAC_w
Cuts at: 0 62 1584
Size: 62 1522
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1284 1316 1546 1578 1584
Size: 1284 32 230 32 6
Fragments arranged by size:
1284 230 32 32 6
Tth111I GACn'n_nGTC
Cuts at: 0 793 1584
Size: 793 791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1396 1584
Size: 1396 188
XmnI GAAnn'nnTTC
Cuts at: 0 1381 1584
Size: 1381 203
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AloI AlwNI
ApaI AvaI BanI BanII BbsI BbvCI BcgI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrFI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DrdI EaeI EarI EciI Eco57MI EcoNI
EcoO109I FalI FseI FspI HaeII Hin4I MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PfoI
PpiI PpuMI PshAI PspOMI PstI PvuII ScaI SexAI
SfcI SnaBI StuI StyI TaqII TatI TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AlfI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BciVI BclI BfrBI BglII BlpI BmtI BpuEI BsaI
BsaBI BsaHI BsiEI BsiWI BsmI BspHI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I ClaI CspCI DraI
DraIII EagI EcoICRI EcoRI EcoRV FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI SspI SwaI XbaI XhoI
XmaI ZraI