Restriction map for
pFN21AE5660
(Linear) (Six-base) MAPSORT of: KIEE5660.seq Check: 1399 from: 1 to: 1524
KIEE5660.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 157 1524
Size: 157 1367
AccI GT'mk_AC
Cuts at: 0 425 1524
Size: 425 1099
Acc65I G'GTAC_C
Cuts at: 0 688 728 1524
Size: 688 40 796
Fragments arranged by size:
796 688 40
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 1137 1524
Size: 144 993 387
Fragments arranged by size:
993 387 144
AlwNI CAG_nnn'CTG
Cuts at: 0 208 1524
Size: 208 1316
ApoI r'AATT_y
Cuts at: 0 188 1524
Size: 188 1336
AvaI C'yCGr_G
Cuts at: 0 698 1524
Size: 698 826
BanI G'GyrC_C
Cuts at: 0 500 688 728 1064 1308 1524
Size: 500 188 40 336 244 216
Fragments arranged by size:
500 336 244 216 188 40
BanII G_rGCy'C
Cuts at: 0 764 1044 1524
Size: 764 280 480
Fragments arranged by size:
764 480 280
BbsI GAAGACnn'nnnn_
Cuts at: 0 1242 1446 1524
Size: 1242 204 78
Fragments arranged by size:
1242 204 78
BglI GCCn_nnn'nGGC
Cuts at: 0 278 1524
Size: 278 1246
BglII A'GATC_T
Cuts at: 0 1191 1524
Size: 1191 333
Bme1580I G_kGCm'C
Cuts at: 0 339 791 1311 1524
Size: 339 452 520 213
Fragments arranged by size:
520 452 339 213
BmrI ACTGGGnnnn_n'
Cuts at: 0 1390 1408 1524
Size: 1390 18 116
Fragments arranged by size:
1390 116 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 230 934 1524
Size: 214 16 704 590
Fragments arranged by size:
704 590 214 16
Bpu10I CC'TnA_GC
Cuts at: 0 1043 1524
Size: 1043 481
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 465 922 1524
Size: 465 457 602
Fragments arranged by size:
602 465 457
BsaI GGTCTCn'nnnn_
Cuts at: 0 224 1524
Size: 224 1300
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 672 702 938 968 1524
Size: 672 30 236 30 556
Fragments arranged by size:
672 556 236 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 604 673 946 1524
Size: 604 69 273 578
Fragments arranged by size:
604 578 273 69
BseYI C'CCAG_C
Cuts at: 0 15 1445 1524
Size: 15 1430 79
Fragments arranged by size:
1430 79 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 798 1498 1524
Size: 798 700 26
Fragments arranged by size:
798 700 26
BsiHKAI G_wGCw'C
Cuts at: 0 412 1409 1524
Size: 412 997 115
Fragments arranged by size:
997 412 115
BsmI GAATG_Cn'
Cuts at: 0 1439 1524
Size: 1439 85
Bsp1286I G_dGCh'C
Cuts at: 0 339 412 764 791 1044 1311 1409 1524
Size: 339 73 352 27 253 267 98 115
Fragments arranged by size:
352 339 267 253 115 98 73 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 157 1440 1524
Size: 157 1283 84
Fragments arranged by size:
1283 157 84
BsrBI CCG'CTC
Cuts at: 0 906 1524
Size: 906 618
BstXI CCAn_nnnn'nTGG
Cuts at: 0 226 942 1524
Size: 226 716 582
Fragments arranged by size:
716 582 226
BstYI r'GATC_y
Cuts at: 0 593 1191 1524
Size: 593 598 333
Fragments arranged by size:
598 593 333
BtgI C'CryG_G
Cuts at: 0 875 1524
Size: 875 649
BtsI GCAGTG_nn'
Cuts at: 0 339 1510 1524
Size: 339 1171 14
Fragments arranged by size:
1171 339 14
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 510 545 1524
Size: 510 35 979
Fragments arranged by size:
979 510 35
DraI TTT'AAA
Cuts at: 0 536 1524
Size: 536 988
DraIII CAC_nnn'GTG
Cuts at: 0 798 1524
Size: 798 726
EaeI y'GGCC_r
Cuts at: 0 543 1524
Size: 543 981
EarI CTCTTCn'nnn_
Cuts at: 0 63 330 364 1156 1524
Size: 63 267 34 792 368
Fragments arranged by size:
792 368 267 63 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 214 230 934 1137 1524
Size: 144 70 16 704 203 387
Fragments arranged by size:
704 387 203 144 70 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 675 1524
Size: 675 849
EcoO109I rG'GnC_Cy
Cuts at: 0 205 253 964 1524
Size: 205 48 711 560
Fragments arranged by size:
711 560 205 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 90 122 1524
Size: 90 32 1402
Fragments arranged by size:
1402 90 32
HincII GTy'rAC
Cuts at: 0 426 1524
Size: 426 1098
KpnI G_GTAC'C
Cuts at: 0 692 732 1524
Size: 692 40 792
Fragments arranged by size:
792 692 40
MfeI C'AATT_G
Cuts at: 0 36 1524
Size: 36 1488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1103 1524
Size: 1103 421
MscI TGG'CCA
Cuts at: 0 545 1524
Size: 545 979
MspA1I CmG'CkG
Cuts at: 0 79 649 1055 1282 1524
Size: 79 570 406 227 242
Fragments arranged by size:
570 406 242 227 79
PflMI CCAn_nnn'nTGG
Cuts at: 0 208 1464 1524
Size: 208 1256 60
Fragments arranged by size:
1256 208 60
PfoI T'CCnGG_A
Cuts at: 0 1457 1524
Size: 1457 67
PpuMI rG'GwC_Cy
Cuts at: 0 205 964 1524
Size: 205 759 560
Fragments arranged by size:
759 560 205
PvuII CAG'CTG
Cuts at: 0 79 649 1055 1282 1524
Size: 79 570 406 227 242
Fragments arranged by size:
570 406 242 227 79
SalI G'TCGA_C
Cuts at: 0 424 1524
Size: 424 1100
SapI GCTCTTCn'nnn_
Cuts at: 0 364 1156 1524
Size: 364 792 368
Fragments arranged by size:
792 368 364
ScaI AGT'ACT
Cuts at: 0 1316 1524
Size: 1316 208
SexAI A'CCwGG_T
Cuts at: 0 1214 1524
Size: 1214 310
SfcI C'TryA_G
Cuts at: 0 5 1004 1524
Size: 5 999 520
Fragments arranged by size:
999 520 5
SmaI CCC'GGG
Cuts at: 0 700 1524
Size: 700 824
SmlI C'TyrA_G
Cuts at: 0 444 901 1524
Size: 444 457 623
Fragments arranged by size:
623 457 444
StuI AGG'CCT
Cuts at: 0 1002 1524
Size: 1002 522
StyI C'CwwG_G
Cuts at: 0 1164 1524
Size: 1164 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 43 518 1524
Size: 43 475 1006
Fragments arranged by size:
1006 475 43
TatI w'GTAC_w
Cuts at: 0 1314 1524
Size: 1314 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 11 43 424 456 938 970 1016 1048
Size: 11 32 381 32 482 32 46 32
Cuts at: 1048 1524
Size: 476
Fragments arranged by size:
482 476 381 46 32 32 32 32
11
Tth111I GACn'n_nGTC
Cuts at: 0 1216 1524
Size: 1216 308
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1206 1371 1486 1524
Size: 1206 165 115 38
Fragments arranged by size:
1206 165 115 38
XmaI C'CCGG_G
Cuts at: 0 698 1524
Size: 698 826
XmnI GAAnn'nnTTC
Cuts at: 0 1249 1524
Size: 1249 275
Enzymes that do cut:
AarI AccI Acc65I AcuI AlwNI ApoI AvaI BanI
BanII BbsI BglI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BstXI BstYI BtgI BtsI CspCI
DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I Hin4I
HincII KpnI MfeI MmeI MscI MspA1I PflMI PfoI
PpuMI PvuII SalI SapI ScaI SexAI SfcI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI MluI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SanDI SbfI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI ZraI