Restriction map for
pFN21AE5658
(Linear) (Six-base) MAPSORT of: KIEE5658.seq Check: 2974 from: 1 to: 1467
KIEE5658.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 583 1467
Size: 583 884
AccI GT'mk_AC
Cuts at: 0 890 1467
Size: 890 577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1405 1467
Size: 1405 62
AhdI GACnn_n'nnGTC
Cuts at: 0 939 1277 1467
Size: 939 338 190
Fragments arranged by size:
939 338 190
AlwNI CAG_nnn'CTG
Cuts at: 0 250 1428 1467
Size: 250 1178 39
Fragments arranged by size:
1178 250 39
AvaI C'yCGr_G
Cuts at: 0 7 1343 1459 1467
Size: 7 1336 116 8
Fragments arranged by size:
1336 116 8 7
BamHI G'GATC_C
Cuts at: 0 911 1467
Size: 911 556
BanI G'GyrC_C
Cuts at: 0 538 1467
Size: 538 929
BanII G_rGCy'C
Cuts at: 0 8 1467
Size: 8 1459
BbeI G_GCGC'C
Cuts at: 0 542 1467
Size: 542 925
BbvCI CC'TCA_GC
Cuts at: 0 369 1467
Size: 369 1098
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1321 1355 1467
Size: 1321 34 112
Fragments arranged by size:
1321 112 34
BglI GCCn_nnn'nGGC
Cuts at: 0 427 1445 1462 1467
Size: 427 1018 17 5
Fragments arranged by size:
1018 427 17 5
BglII A'GATC_T
Cuts at: 0 545 779 821 1467
Size: 545 234 42 646
Fragments arranged by size:
646 545 234 42
BlpI GC'TnA_GC
Cuts at: 0 1435 1467
Size: 1435 32
Bme1580I G_kGCm'C
Cuts at: 0 325 1467
Size: 325 1142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 63 286 366 649 834 1108 1303
Size: 34 29 223 80 283 185 274 195
Cuts at: 1303 1467
Size: 164
Fragments arranged by size:
283 274 223 195 185 164 80 34
29
Bpu10I CC'TnA_GC
Cuts at: 0 369 1467
Size: 369 1098
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 284 967 1467
Size: 192 92 683 500
Fragments arranged by size:
683 500 192 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 802 858 1372 1407 1467
Size: 802 56 514 35 60
Fragments arranged by size:
802 514 60 56 35
BsaHI Gr'CG_yC
Cuts at: 0 539 1467
Size: 539 928
BsaWI w'CCGG_w
Cuts at: 0 908 1467
Size: 908 559
BseYI C'CCAG_C
Cuts at: 0 417 507 512 1217 1382 1467
Size: 417 90 5 705 165 85
Fragments arranged by size:
705 417 165 90 85 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 976 1094 1127 1467
Size: 976 118 33 340
Fragments arranged by size:
976 340 118 33
BsiHKAI G_wGCw'C
Cuts at: 0 20 572 1436 1467
Size: 20 552 864 31
Fragments arranged by size:
864 552 31 20
Bsp1286I G_dGCh'C
Cuts at: 0 8 20 325 572 1436 1467
Size: 8 12 305 247 864 31
Fragments arranged by size:
864 305 247 31 12 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 583 836 1341 1467
Size: 583 253 505 126
Fragments arranged by size:
583 505 253 126
BsrFI r'CCGG_y
Cuts at: 0 1130 1467
Size: 1130 337
BsrGI T'GTAC_A
Cuts at: 0 361 1467
Size: 361 1106
BssSI C'ACGA_G
Cuts at: 0 1309 1467
Size: 1309 158
BstAPI GCAn_nnn'nTGC
Cuts at: 0 24 1467
Size: 24 1443
BstXI CCAn_nnnn'nTGG
Cuts at: 0 264 294 338 1467
Size: 264 30 44 1129
Fragments arranged by size:
1129 264 44 30
BstYI r'GATC_y
Cuts at: 0 545 779 821 911 1467
Size: 545 234 42 90 556
Fragments arranged by size:
556 545 234 90 42
Bsu36I CC'TnA_GG
Cuts at: 0 1261 1467
Size: 1261 206
BtgI C'CryG_G
Cuts at: 0 147 407 587 1467
Size: 147 260 180 880
Fragments arranged by size:
880 260 180 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 736 901 1467
Size: 736 165 566
Fragments arranged by size:
736 566 165
BtsI GCAGTG_nn'
Cuts at: 0 22 1144 1467
Size: 22 1122 323
Fragments arranged by size:
1122 323 22
ClaI AT'CG_AT
Cuts at: 0 1451 1467
Size: 1451 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1146 1181 1467
Size: 1146 35 286
Fragments arranged by size:
1146 286 35
EaeI y'GGCC_r
Cuts at: 0 109 404 659 1467
Size: 109 295 255 808
Fragments arranged by size:
808 295 255 109
EarI CTCTTCn'nnn_
Cuts at: 0 455 1467
Size: 455 1012
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 63 286 366 649 834 1108 1303
Size: 34 29 223 80 283 185 274 195
Cuts at: 1303 1405 1467
Size: 102 62
Fragments arranged by size:
283 274 223 195 185 102 80 62
34 29
EcoO109I rG'GnC_Cy
Cuts at: 0 32 229 492 764 1467
Size: 32 197 263 272 703
Fragments arranged by size:
703 272 263 197 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 660 663 723 1467
Size: 660 3 60 744
Fragments arranged by size:
744 660 60 3
FspI TGC'GCA
Cuts at: 0 28 1467
Size: 28 1439
HaeII r_GCGC'y
Cuts at: 0 542 1467
Size: 542 925
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 925 957 1263 1295 1371 1403 1467
Size: 925 32 306 32 76 32 64
Fragments arranged by size:
925 306 76 64 32 32 32
HincII GTy'rAC
Cuts at: 0 1158 1467
Size: 1158 309
KasI G'GCGC_C
Cuts at: 0 538 1467
Size: 538 929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1436 1467
Size: 1436 31
MscI TGG'CCA
Cuts at: 0 111 406 661 1467
Size: 111 295 255 806
Fragments arranged by size:
806 295 255 111
MslI CAynn'nnrTG
Cuts at: 0 742 1123 1246 1467
Size: 742 381 123 221
Fragments arranged by size:
742 381 221 123
MspA1I CmG'CkG
Cuts at: 0 121 247 373 1467
Size: 121 126 126 1094
Fragments arranged by size:
1094 126 126 121
NarI GG'CG_CC
Cuts at: 0 539 1467
Size: 539 928
NcoI C'CATG_G
Cuts at: 0 587 1467
Size: 587 880
NspI r_CATG'y
Cuts at: 0 555 1467
Size: 555 912
PflMI CCAn_nnn'nTGG
Cuts at: 0 1050 1467
Size: 1050 417
PfoI T'CCnGG_A
Cuts at: 0 40 1467
Size: 40 1427
PpuMI rG'GwC_Cy
Cuts at: 0 32 229 764 1467
Size: 32 197 535 703
Fragments arranged by size:
703 535 197 32
PstI C_TGCA'G
Cuts at: 0 378 1467
Size: 378 1089
PvuII CAG'CTG
Cuts at: 0 121 247 373 1467
Size: 121 126 126 1094
Fragments arranged by size:
1094 126 126 121
SexAI A'CCwGG_T
Cuts at: 0 213 1467
Size: 213 1254
SfcI C'TryA_G
Cuts at: 0 374 759 1467
Size: 374 385 708
Fragments arranged by size:
708 385 374
SfoI GGC'GCC
Cuts at: 0 540 1467
Size: 540 927
SmaI CCC'GGG
Cuts at: 0 9 1467
Size: 9 1458
SmlI C'TyrA_G
Cuts at: 0 171 299 982 1467
Size: 171 128 683 485
Fragments arranged by size:
683 485 171 128
SrfI GCCC'GGGC
Cuts at: 0 9 1467
Size: 9 1458
StuI AGG'CCT
Cuts at: 0 430 452 468 643 1467
Size: 430 22 16 175 824
Fragments arranged by size:
824 430 175 22 16
StyI C'CwwG_G
Cuts at: 0 51 293 587 1467
Size: 51 242 294 880
Fragments arranged by size:
880 294 242 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1113 1467
Size: 1113 354
TatI w'GTAC_w
Cuts at: 0 361 1467
Size: 361 1106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 335 1467
Size: 335 1132
XmaI C'CCGG_G
Cuts at: 0 7 1467
Size: 7 1460
XmnI GAAnn'nnTTC
Cuts at: 0 643 1467
Size: 643 824
Enzymes that do cut:
AarI AccI AcuI AhdI AlwNI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BglI BglII BlpI Bme1580I
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrFI BsrGI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI EaeI
EarI Eco57MI EcoO109I FalI FspI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NcoI NspI
PflMI PfoI PpuMI PstI PvuII SexAI SfcI SfoI
SmaI SmlI SrfI StuI StyI TaqII TatI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbsI BciVI BclI BfrBI BmgBI BmrI
BmtI BplI BsaAI BsaBI BsaXI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BssHII BstBI
BstEII BstZ17I DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI TstI Tth111I XbaI
XhoI ZraI