Restriction map for
pFN21AE5657
(Linear) (Six-base) MAPSORT of: KIEE5657.seq Check: 9733 from: 1 to: 1449
KIEE5657.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AclI AA'CG_TT
Cuts at: 0 963 1449
Size: 963 486
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1449
Size: 583 866
AfeI AGC'GCT
Cuts at: 0 129 1449
Size: 129 1320
AlwNI CAG_nnn'CTG
Cuts at: 0 282 561 1449
Size: 282 279 888
Fragments arranged by size:
888 282 279
ApaI G_GGCC'C
Cuts at: 0 43 56 1449
Size: 43 13 1393
Fragments arranged by size:
1393 43 13
AvaI C'yCGr_G
Cuts at: 0 123 1449
Size: 123 1326
AvrII C'CTAG_G
Cuts at: 0 165 1111 1449
Size: 165 946 338
Fragments arranged by size:
946 338 165
BanI G'GyrC_C
Cuts at: 0 91 375 1449
Size: 91 284 1074
Fragments arranged by size:
1074 284 91
BanII G_rGCy'C
Cuts at: 0 43 56 480 1449
Size: 43 13 424 969
Fragments arranged by size:
969 424 43 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 391 953 1436 1449
Size: 391 562 483 13
Fragments arranged by size:
562 483 391 13
BciVI GTATCCnnnnn_n'
Cuts at: 0 630 1449
Size: 630 819
BclI T'GATC_A
Cuts at: 0 1416 1449
Size: 1416 33
BglI GCCn_nnn'nGGC
Cuts at: 0 47 1449
Size: 47 1402
BglII A'GATC_T
Cuts at: 0 880 1057 1449
Size: 880 177 392
Fragments arranged by size:
880 392 177
Bme1580I G_kGCm'C
Cuts at: 0 43 56 1449
Size: 43 13 1393
Fragments arranged by size:
1393 43 13
BmtI G_CTAG'C
Cuts at: 0 134 1449
Size: 134 1315
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 794 899 1449
Size: 794 105 550
Fragments arranged by size:
794 550 105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1449
Size: 539 910
BsaI GGTCTCn'nnnn_
Cuts at: 0 616 1082 1449
Size: 616 466 367
Fragments arranged by size:
616 466 367
BsaBI GATnn'nnATC
Cuts at: 0 753 1418 1449
Size: 753 665 31
Fragments arranged by size:
753 665 31
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1044 1074 1449
Size: 1044 30 375
Fragments arranged by size:
1044 375 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 783 786 1449
Size: 783 3 663
Fragments arranged by size:
783 663 3
BseYI C'CCAG_C
Cuts at: 0 22 43 1449
Size: 22 21 1406
Fragments arranged by size:
1406 22 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 1449
Size: 488 961
BsiHKAI G_wGCw'C
Cuts at: 0 919 1449
Size: 919 530
BsmI GAATG_Cn'
Cuts at: 0 741 1449
Size: 741 708
BsmBI CGTCTCn'nnnn_
Cuts at: 0 813 1020 1449
Size: 813 207 429
Fragments arranged by size:
813 429 207
Bsp1286I G_dGCh'C
Cuts at: 0 43 56 480 919 1449
Size: 43 13 424 439 530
Fragments arranged by size:
530 439 424 43 13
BspHI T'CATG_A
Cuts at: 0 1422 1449
Size: 1422 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 22 385 1449
Size: 22 363 1064
Fragments arranged by size:
1064 363 22
BsrBI CCG'CTC
Cuts at: 0 1100 1449
Size: 1100 349
BssSI C'ACGA_G
Cuts at: 0 227 1449
Size: 227 1222
BstYI r'GATC_y
Cuts at: 0 880 1057 1449
Size: 880 177 392
Fragments arranged by size:
880 392 177
BtsI GCAGTG_nn'
Cuts at: 0 26 395 1449
Size: 26 369 1054
Fragments arranged by size:
1054 369 26
EarI CTCTTCn'nnn_
Cuts at: 0 160 196 1192 1271 1310 1449
Size: 160 36 996 79 39 139
Fragments arranged by size:
996 160 139 79 39 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 77 95 888 1449
Size: 77 18 793 561
Fragments arranged by size:
793 561 77 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 583 1449
Size: 539 44 866
Fragments arranged by size:
866 539 44
EcoO109I rG'GnC_Cy
Cuts at: 0 40 1449
Size: 40 1409
HaeII r_GCGC'y
Cuts at: 0 131 455 1449
Size: 131 324 994
Fragments arranged by size:
994 324 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 135 167 244 276 1449
Size: 135 32 77 32 1173
Fragments arranged by size:
1173 135 77 32 32
HincII GTy'rAC
Cuts at: 0 303 1449
Size: 303 1146
HindIII A'AGCT_T
Cuts at: 0 359 565 1449
Size: 359 206 884
Fragments arranged by size:
884 359 206
MslI CAynn'nnrTG
Cuts at: 0 1438 1449
Size: 1438 11
MspA1I CmG'CkG
Cuts at: 0 47 1449
Size: 47 1402
NdeI CA'TA_TG
Cuts at: 0 327 1449
Size: 327 1122
NheI G'CTAG_C
Cuts at: 0 130 1449
Size: 130 1319
PfoI T'CCnGG_A
Cuts at: 0 516 1449
Size: 516 933
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 173 205 1449
Size: 173 32 1244
Fragments arranged by size:
1244 173 32
PshAI GACnn'nnGTC
Cuts at: 0 115 825 1449
Size: 115 710 624
Fragments arranged by size:
710 624 115
PspOMI G'GGCC_C
Cuts at: 0 39 52 1449
Size: 39 13 1397
Fragments arranged by size:
1397 39 13
PstI C_TGCA'G
Cuts at: 0 33 552 1449
Size: 33 519 897
Fragments arranged by size:
897 519 33
ScaI AGT'ACT
Cuts at: 0 576 1449
Size: 576 873
SfcI C'TryA_G
Cuts at: 0 29 548 707 1449
Size: 29 519 159 742
Fragments arranged by size:
742 519 159 29
StyI C'CwwG_G
Cuts at: 0 165 868 1111 1449
Size: 165 703 243 338
Fragments arranged by size:
703 338 243 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1064 1449
Size: 1064 385
TatI w'GTAC_w
Cuts at: 0 574 1449
Size: 574 875
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 18 50 1044 1076 1449
Size: 18 32 994 32 373
Fragments arranged by size:
994 373 32 32 18
XbaI T'CTAG_A
Cuts at: 0 790 1006 1449
Size: 790 216 443
Fragments arranged by size:
790 443 216
XmnI GAAnn'nnTTC
Cuts at: 0 741 1449
Size: 741 708
Enzymes that do cut:
AclI AcuI AfeI AlwNI ApaI AvaI AvrII BanI
BanII BbsI BciVI BclI BglI BglII Bme1580I BmtI
BplI BpmI BsaI BsaBI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BssSI
BstYI BtsI EarI EciI Eco57MI EcoO109I HaeII Hin4I
HincII HindIII MslI MspA1I NdeI NheI PfoI PpiI
PshAI PspOMI PstI ScaI SfcI StyI TaqII TatI
TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
BaeI BamHI BbeI BbvCI BcgI BfrBI BlpI BmgBI
BmrI Bpu10I BpuEI BsaAI BsaHI BsaWI BsiEI BsiWI
BspEI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NgoMIV NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpuMI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XcmI XhoI XmaI
ZraI