Restriction map for
pFN21AE5656
(Linear) (Six-base) MAPSORT of: KIEE5656.seq Check: 9194 from: 1 to: 1434
KIEE5656.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 907 1434
Size: 592 315 527
Fragments arranged by size:
592 527 315
AfeI AGC'GCT
Cuts at: 0 1129 1434
Size: 1129 305
AflIII A'CryG_T
Cuts at: 0 1169 1434
Size: 1169 265
AleI CACnn'nnGTG
Cuts at: 0 1168 1434
Size: 1168 266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1130 1434
Size: 1130 304
AlwNI CAG_nnn'CTG
Cuts at: 0 396 492 797 807 1434
Size: 396 96 305 10 627
Fragments arranged by size:
627 396 305 96 10
ApaI G_GGCC'C
Cuts at: 0 21 1434
Size: 21 1413
ApoI r'AATT_y
Cuts at: 0 556 1434
Size: 556 878
BamHI G'GATC_C
Cuts at: 0 243 1434
Size: 243 1191
BanI G'GyrC_C
Cuts at: 0 643 1434
Size: 643 791
BanII G_rGCy'C
Cuts at: 0 21 1055 1434
Size: 21 1034 379
Fragments arranged by size:
1034 379 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 836 1434
Size: 836 598
BciVI GTATCCnnnnn_n'
Cuts at: 0 903 1434
Size: 903 531
BclI T'GATC_A
Cuts at: 0 191 1434
Size: 191 1243
BglI GCCn_nnn'nGGC
Cuts at: 0 1295 1434
Size: 1295 139
BlpI GC'TnA_GC
Cuts at: 0 454 1434
Size: 454 980
Bme1580I G_kGCm'C
Cuts at: 0 21 1179 1434
Size: 21 1158 255
Fragments arranged by size:
1158 255 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 165 399 480 1434
Size: 165 234 81 954
Fragments arranged by size:
954 234 165 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1434
Size: 292 1142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 983 1434
Size: 664 319 451
Fragments arranged by size:
664 451 319
BsaI GGTCTCn'nnnn_
Cuts at: 0 895 1252 1434
Size: 895 357 182
Fragments arranged by size:
895 357 182
BsaWI w'CCGG_w
Cuts at: 0 1271 1434
Size: 1271 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 290 320 655 685 1434
Size: 290 30 335 30 749
Fragments arranged by size:
749 335 290 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 1434
Size: 163 1271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 957 1252 1434
Size: 957 295 182
Fragments arranged by size:
957 295 182
BsiHKAI G_wGCw'C
Cuts at: 0 673 1055 1434
Size: 673 382 379
Fragments arranged by size:
673 382 379
BsmBI CGTCTCn'nnnn_
Cuts at: 0 941 1434
Size: 941 493
Bsp1286I G_dGCh'C
Cuts at: 0 21 673 1055 1179 1434
Size: 21 652 382 124 255
Fragments arranged by size:
652 382 255 124 21
BspHI T'CATG_A
Cuts at: 0 734 1434
Size: 734 700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1274 1434
Size: 1274 160
BsrDI GCAATG_nn'
Cuts at: 0 992 1434
Size: 992 442
BssSI C'ACGA_G
Cuts at: 0 12 1434
Size: 12 1422
BstEII G'GTnAC_C
Cuts at: 0 1158 1434
Size: 1158 276
BstYI r'GATC_y
Cuts at: 0 243 1434
Size: 243 1191
BtgI C'CryG_G
Cuts at: 0 323 1179 1229 1434
Size: 323 856 50 205
Fragments arranged by size:
856 323 205 50
BtsI GCAGTG_nn'
Cuts at: 0 125 1319 1434
Size: 125 1194 115
Fragments arranged by size:
1194 125 115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 206 241 1434
Size: 206 35 1193
Fragments arranged by size:
1193 206 35
DraIII CAC_nnn'GTG
Cuts at: 0 1074 1434
Size: 1074 360
EaeI y'GGCC_r
Cuts at: 0 2 1434
Size: 2 1432
EarI CTCTTCn'nnn_
Cuts at: 0 74 140 275 893 1434
Size: 74 66 135 618 541
Fragments arranged by size:
618 541 135 74 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 807 1434
Size: 807 627
EcoICRI GAG'CTC
Cuts at: 0 1053 1434
Size: 1053 381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 592 907 1434
Size: 292 300 315 527
Fragments arranged by size:
527 315 300 292
EcoO109I rG'GnC_Cy
Cuts at: 0 18 1305 1434
Size: 18 1287 129
Fragments arranged by size:
1287 129 18
HaeII r_GCGC'y
Cuts at: 0 523 1131 1434
Size: 523 608 303
Fragments arranged by size:
608 523 303
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 655 687 729 761 1434
Size: 235 32 388 32 42 32 673
Fragments arranged by size:
673 388 235 42 32 32 32
HindIII A'AGCT_T
Cuts at: 0 574 1434
Size: 574 860
MscI TGG'CCA
Cuts at: 0 4 1434
Size: 4 1430
MslI CAynn'nnrTG
Cuts at: 0 1168 1434
Size: 1168 266
MspA1I CmG'CkG
Cuts at: 0 433 1181 1434
Size: 433 748 253
Fragments arranged by size:
748 433 253
NcoI C'CATG_G
Cuts at: 0 323 1434
Size: 323 1111
NdeI CA'TA_TG
Cuts at: 0 510 1434
Size: 510 924
PasI CC'CwG_GG
Cuts at: 0 649 1434
Size: 649 785
PflMI CCAn_nnn'nTGG
Cuts at: 0 858 1074 1434
Size: 858 216 360
Fragments arranged by size:
858 360 216
PfoI T'CCnGG_A
Cuts at: 0 1361 1434
Size: 1361 73
PspOMI G'GGCC_C
Cuts at: 0 17 1434
Size: 17 1417
PvuII CAG'CTG
Cuts at: 0 433 1434
Size: 433 1001
SacI G_AGCT'C
Cuts at: 0 1055 1434
Size: 1055 379
SacII CC_GC'GG
Cuts at: 0 1182 1434
Size: 1182 252
SapI GCTCTTCn'nnn_
Cuts at: 0 275 1434
Size: 275 1159
ScaI AGT'ACT
Cuts at: 0 925 1434
Size: 925 509
SexAI A'CCwGG_T
Cuts at: 0 1070 1434
Size: 1070 364
SmlI C'TyrA_G
Cuts at: 0 679 998 1434
Size: 679 319 436
Fragments arranged by size:
679 436 319
StuI AGG'CCT
Cuts at: 0 964 1434
Size: 964 470
StyI C'CwwG_G
Cuts at: 0 323 1434
Size: 323 1111
TatI w'GTAC_w
Cuts at: 0 260 923 1434
Size: 260 663 511
Fragments arranged by size:
663 511 260
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 290 322 1434
Size: 290 32 1112
Fragments arranged by size:
1112 290 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 412 1434
Size: 412 1022
XmnI GAAnn'nnTTC
Cuts at: 0 284 1434
Size: 284 1150
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApoI
BamHI BanI BanII BbsI BciVI BclI BglI BlpI
Bme1580I BmrI BpmI BpuEI BsaI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BssSI
BstEII BstYI BtgI BtsI CspCI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HindIII MscI
MslI MspA1I NcoI NdeI PasI PflMI PfoI PspOMI
PvuII SacI SacII SapI ScaI SexAI SmlI StuI
StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BbvCI BcgI BfrBI BglII BmgBI BmtI BplI
Bpu10I BsaAI BsaBI BsaHI BseYI BsiEI BsiWI BsmI
BspEI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstXI
BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SalI SanDI SbfI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII Tth111I XbaI XhoI XmaI ZraI