Restriction map for
pFN21AE5653
(Linear) (Six-base) MAPSORT of: KIEE5653.seq Check: 9382 from: 1 to: 1518
KIEE5653.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
Acc65I G'GTAC_C
Cuts at: 0 231 1518
Size: 231 1287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1518
Size: 817 701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 315 545 1482 1518
Size: 315 230 937 36
Fragments arranged by size:
937 315 230 36
AlwNI CAG_nnn'CTG
Cuts at: 0 1096 1518
Size: 1096 422
ApaLI G'TGCA_C
Cuts at: 0 73 371 1518
Size: 73 298 1147
Fragments arranged by size:
1147 298 73
ApoI r'AATT_y
Cuts at: 0 28 58 805 1518
Size: 28 30 747 713
Fragments arranged by size:
747 713 30 28
AvaI C'yCGr_G
Cuts at: 0 557 1518
Size: 557 961
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 721 754 1518
Size: 721 33 764
Fragments arranged by size:
764 721 33
BanI G'GyrC_C
Cuts at: 0 231 1518
Size: 231 1287
BanII G_rGCy'C
Cuts at: 0 228 558 1403 1518
Size: 228 330 845 115
Fragments arranged by size:
845 330 228 115
BbsI GAAGACnn'nnnn_
Cuts at: 0 782 1518
Size: 782 736
BbvCI CC'TCA_GC
Cuts at: 0 518 1208 1214 1518
Size: 518 690 6 304
Fragments arranged by size:
690 518 304 6
Bme1580I G_kGCm'C
Cuts at: 0 77 375 1387 1518
Size: 77 298 1012 131
Fragments arranged by size:
1012 298 131 77
BmtI G_CTAG'C
Cuts at: 0 359 1379 1518
Size: 359 1020 139
Fragments arranged by size:
1020 359 139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 953 1518
Size: 196 757 565
Fragments arranged by size:
757 565 196
Bpu10I CC'TnA_GC
Cuts at: 0 518 1208 1214 1518
Size: 518 690 6 304
Fragments arranged by size:
690 518 304 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1518
Size: 627 891
BsaI GGTCTCn'nnnn_
Cuts at: 0 1195 1518
Size: 1195 323
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 215 245 1518
Size: 215 30 1273
Fragments arranged by size:
1273 215 30
BseYI C'CCAG_C
Cuts at: 0 1029 1518
Size: 1029 489
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 691 1466 1518
Size: 28 663 775 52
Fragments arranged by size:
775 663 52 28
BsiHKAI G_wGCw'C
Cuts at: 0 77 375 1403 1518
Size: 77 298 1028 115
Fragments arranged by size:
1028 298 115 77
Bsp1286I G_dGCh'C
Cuts at: 0 77 228 375 558 1387 1403 1518
Size: 77 151 147 183 829 16 115
Fragments arranged by size:
829 183 151 147 115 77 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 127 1518
Size: 127 1391
BsrDI GCAATG_nn'
Cuts at: 0 1136 1518
Size: 1136 382
BsrGI T'GTAC_A
Cuts at: 0 1291 1389 1518
Size: 1291 98 129
Fragments arranged by size:
1291 129 98
BssSI C'ACGA_G
Cuts at: 0 1277 1518
Size: 1277 241
BstAPI GCAn_nnn'nTGC
Cuts at: 0 119 1518
Size: 119 1399
BstEII G'GTnAC_C
Cuts at: 0 759 1024 1518
Size: 759 265 494
Fragments arranged by size:
759 494 265
BstXI CCAn_nnnn'nTGG
Cuts at: 0 220 1518
Size: 220 1298
Bsu36I CC'TnA_GG
Cuts at: 0 695 1518
Size: 695 823
BtgI C'CryG_G
Cuts at: 0 863 1335 1518
Size: 863 472 183
Fragments arranged by size:
863 472 183
BtsI GCAGTG_nn'
Cuts at: 0 1454 1518
Size: 1454 64
DraIII CAC_nnn'GTG
Cuts at: 0 337 1518
Size: 337 1181
EarI CTCTTCn'nnn_
Cuts at: 0 587 786 1307 1479 1505 1518
Size: 587 199 521 172 26 13
Fragments arranged by size:
587 521 199 172 26 13
EcoICRI GAG'CTC
Cuts at: 0 1401 1518
Size: 1401 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 817 953 1518
Size: 196 621 136 565
Fragments arranged by size:
621 565 196 136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 138 1518
Size: 138 1380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 763 795 1518
Size: 229 32 502 32 723
Fragments arranged by size:
723 502 229 32 32
HincII GTy'rAC
Cuts at: 0 198 421 1518
Size: 198 223 1097
Fragments arranged by size:
1097 223 198
HindIII A'AGCT_T
Cuts at: 0 143 1518
Size: 143 1375
KpnI G_GTAC'C
Cuts at: 0 235 1518
Size: 235 1283
MfeI C'AATT_G
Cuts at: 0 1004 1518
Size: 1004 514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 1518
Size: 201 1317
MspA1I CmG'CkG
Cuts at: 0 547 795 1119 1518
Size: 547 248 324 399
Fragments arranged by size:
547 399 324 248
NcoI C'CATG_G
Cuts at: 0 863 1518
Size: 863 655
NheI G'CTAG_C
Cuts at: 0 355 1375 1518
Size: 355 1020 143
Fragments arranged by size:
1020 355 143
NspI r_CATG'y
Cuts at: 0 942 1245 1469 1518
Size: 942 303 224 49
Fragments arranged by size:
942 303 224 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 337 539 1518
Size: 337 202 979
Fragments arranged by size:
979 337 202
PfoI T'CCnGG_A
Cuts at: 0 992 1518
Size: 992 526
PspXI vC'TCGA_Gb
Cuts at: 0 557 1518
Size: 557 961
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 485 517 1275 1307 1518
Size: 485 32 758 32 211
Fragments arranged by size:
758 485 211 32 32
PvuII CAG'CTG
Cuts at: 0 795 1119 1518
Size: 795 324 399
Fragments arranged by size:
795 399 324
SacI G_AGCT'C
Cuts at: 0 1403 1518
Size: 1403 115
SapI GCTCTTCn'nnn_
Cuts at: 0 1307 1518
Size: 1307 211
SfcI C'TryA_G
Cuts at: 0 961 1518
Size: 961 557
SmlI C'TyrA_G
Cuts at: 0 557 642 1518
Size: 557 85 876
Fragments arranged by size:
876 557 85
StuI AGG'CCT
Cuts at: 0 598 1053 1518
Size: 598 455 465
Fragments arranged by size:
598 465 455
StyI C'CwwG_G
Cuts at: 0 471 863 1518
Size: 471 392 655
Fragments arranged by size:
655 471 392
TatI w'GTAC_w
Cuts at: 0 186 1291 1389 1518
Size: 186 1105 98 129
Fragments arranged by size:
1105 186 129 98
XhoI C'TCGA_G
Cuts at: 0 557 1518
Size: 557 961
Enzymes that do cut:
Acc65I AcuI AlfI AlwNI ApaLI ApoI AvaI BaeI
BanI BanII BbsI BbvCI Bme1580I BmtI BpmI Bpu10I
BpuEI BsaI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BsrGI BssSI BstAPI BstEII BstXI Bsu36I BtgI
BtsI DraIII EarI EcoICRI Eco57MI FalI Hin4I HincII
HindIII KpnI MfeI MmeI MspA1I NcoI NheI NspI
PflMI PfoI PspXI PsrI PvuII SacI SapI SfcI
SmlI StuI StyI TatI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BamHI BbeI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BplI BsaAI BsaBI BsaHI BsaWI
BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BssHII BstBI BstYI BstZ17I BtgZI ClaI CspCI
DraI DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII HpaI KasI MluI
MscI MslI NaeI NarI NdeI NgoMIV NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TstI Tth111I XbaI XcmI XmaI XmnI ZraI