Restriction map for
pFN21AE5650
(Linear) (Six-base) MAPSORT of: KIEE5650.seq Check: 3924 from: 1 to: 1494
KIEE5650.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AccI GT'mk_AC
Cuts at: 0 260 526 1494
Size: 260 266 968
Fragments arranged by size:
968 266 260
AclI AA'CG_TT
Cuts at: 0 1043 1494
Size: 1043 451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1494
Size: 703 791
AfeI AGC'GCT
Cuts at: 0 268 1494
Size: 268 1226
AflII C'TTAA_G
Cuts at: 0 1078 1494
Size: 1078 416
AflIII A'CryG_T
Cuts at: 0 356 743 1494
Size: 356 387 751
Fragments arranged by size:
751 387 356
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 844 876 1494
Size: 844 32 618
Fragments arranged by size:
844 618 32
AlwNI CAG_nnn'CTG
Cuts at: 0 288 447 591 723 1494
Size: 288 159 144 132 771
Fragments arranged by size:
771 288 159 144 132
AvaI C'yCGr_G
Cuts at: 0 760 1494
Size: 760 734
AvrII C'CTAG_G
Cuts at: 0 918 1494
Size: 918 576
BamHI G'GATC_C
Cuts at: 0 1452 1494
Size: 1452 42
BanI G'GyrC_C
Cuts at: 0 366 1494
Size: 366 1128
BanII G_rGCy'C
Cuts at: 0 823 1494
Size: 823 671
BbsI GAAGACnn'nnnn_
Cuts at: 0 636 1064 1494
Size: 636 428 430
Fragments arranged by size:
636 430 428
BbvCI CC'TCA_GC
Cuts at: 0 1153 1494
Size: 1153 341
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 871 905 1494
Size: 871 34 589
Fragments arranged by size:
871 589 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 523 1494
Size: 523 971
BclI T'GATC_A
Cuts at: 0 936 1494
Size: 936 558
Bme1580I G_kGCm'C
Cuts at: 0 371 641 1140 1494
Size: 371 270 499 354
Fragments arranged by size:
499 371 354 270
BmrI ACTGGGnnnn_n'
Cuts at: 0 789 1494
Size: 789 705
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 1494
Size: 424 1070
Bpu10I CC'TnA_GC
Cuts at: 0 1153 1494
Size: 1153 341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 1313 1494
Size: 945 368 181
Fragments arranged by size:
945 368 181
BsaAI yAC'GTr
Cuts at: 0 75 1494
Size: 75 1419
BsaBI GATnn'nnATC
Cuts at: 0 511 941 1494
Size: 511 430 553
Fragments arranged by size:
553 511 430
BsaHI Gr'CG_yC
Cuts at: 0 1091 1494
Size: 1091 403
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 844 874 1494
Size: 844 30 620
Fragments arranged by size:
844 620 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 865 898 1350 1494
Size: 865 33 452 144
Fragments arranged by size:
865 452 144 33
BseYI C'CCAG_C
Cuts at: 0 63 274 281 597 1494
Size: 63 211 7 316 897
Fragments arranged by size:
897 316 211 63 7
BsiEI CG_ry'CG
Cuts at: 0 132 1494
Size: 132 1362
BsiHKAI G_wGCw'C
Cuts at: 0 233 1316 1494
Size: 233 1083 178
Fragments arranged by size:
1083 233 178
Bsp1286I G_dGCh'C
Cuts at: 0 233 371 641 823 1140 1316 1494
Size: 233 138 270 182 317 176 178
Fragments arranged by size:
317 270 233 182 178 176 138
BspHI T'CATG_A
Cuts at: 0 137 1494
Size: 137 1357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 125 731 1494
Size: 125 606 763
Fragments arranged by size:
763 606 125
BsrFI r'CCGG_y
Cuts at: 0 182 1494
Size: 182 1312
BsrGI T'GTAC_A
Cuts at: 0 551 1494
Size: 551 943
BssSI C'ACGA_G
Cuts at: 0 1003 1494
Size: 1003 491
BstAPI GCAn_nnn'nTGC
Cuts at: 0 291 1494
Size: 291 1203
BstYI r'GATC_y
Cuts at: 0 1376 1452 1494
Size: 1376 76 42
Fragments arranged by size:
1376 76 42
BstZ17I GTA'TAC
Cuts at: 0 527 1494
Size: 527 967
BtgI C'CryG_G
Cuts at: 0 121 1494
Size: 121 1373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1310 1494
Size: 1310 184
DraIII CAC_nnn'GTG
Cuts at: 0 291 1494
Size: 291 1203
EaeI y'GGCC_r
Cuts at: 0 615 909 1494
Size: 615 294 585
Fragments arranged by size:
615 585 294
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 780 1494
Size: 780 714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 703 1494
Size: 424 279 791
Fragments arranged by size:
791 424 279
EcoO109I rG'GnC_Cy
Cuts at: 0 426 720 1008 1301 1494
Size: 426 294 288 293 193
Fragments arranged by size:
426 294 293 288 193
FspI TGC'GCA
Cuts at: 0 241 1494
Size: 241 1253
HaeII r_GCGC'y
Cuts at: 0 270 281 1195 1494
Size: 270 11 914 299
Fragments arranged by size:
914 299 270 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 404 436 1494
Size: 404 32 1058
Fragments arranged by size:
1058 404 32
HindIII A'AGCT_T
Cuts at: 0 1075 1201 1239 1494
Size: 1075 126 38 255
Fragments arranged by size:
1075 255 126 38
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 1494
Size: 311 1183
MscI TGG'CCA
Cuts at: 0 617 1494
Size: 617 877
MslI CAynn'nnrTG
Cuts at: 0 491 1165 1494
Size: 491 674 329
Fragments arranged by size:
674 491 329
MspA1I CmG'CkG
Cuts at: 0 95 784 1494
Size: 95 689 710
Fragments arranged by size:
710 689 95
NaeI GCC'GGC
Cuts at: 0 184 1494
Size: 184 1310
NgoMIV G'CCGG_C
Cuts at: 0 182 1494
Size: 182 1312
NspI r_CATG'y
Cuts at: 0 360 747 1494
Size: 360 387 747
Fragments arranged by size:
747 387 360
PasI CC'CwG_GG
Cuts at: 0 371 950 1494
Size: 371 579 544
Fragments arranged by size:
579 544 371
PciI A'CATG_T
Cuts at: 0 356 743 1494
Size: 356 387 751
Fragments arranged by size:
751 387 356
PflMI CCAn_nnn'nTGG
Cuts at: 0 288 624 1494
Size: 288 336 870
Fragments arranged by size:
870 336 288
PfoI T'CCnGG_A
Cuts at: 0 422 1494
Size: 422 1072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 844 876 1494
Size: 844 32 618
Fragments arranged by size:
844 618 32
PpuMI rG'GwC_Cy
Cuts at: 0 426 720 1494
Size: 426 294 774
Fragments arranged by size:
774 426 294
PshAI GACnn'nnGTC
Cuts at: 0 82 1494
Size: 82 1412
PstI C_TGCA'G
Cuts at: 0 659 929 1494
Size: 659 270 565
Fragments arranged by size:
659 565 270
PvuII CAG'CTG
Cuts at: 0 784 1494
Size: 784 710
SanDI GG'GwC_CC
Cuts at: 0 426 1494
Size: 426 1068
SexAI A'CCwGG_T
Cuts at: 0 287 1494
Size: 287 1207
SfcI C'TryA_G
Cuts at: 0 655 925 1131 1494
Size: 655 270 206 363
Fragments arranged by size:
655 363 270 206
SmlI C'TyrA_G
Cuts at: 0 960 1078 1328 1494
Size: 960 118 250 166
Fragments arranged by size:
960 250 166 118
StuI AGG'CCT
Cuts at: 0 607 923 1069 1494
Size: 607 316 146 425
Fragments arranged by size:
607 425 316 146
StyI C'CwwG_G
Cuts at: 0 602 918 1466 1494
Size: 602 316 548 28
Fragments arranged by size:
602 548 316 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 354 363 1331 1494
Size: 354 9 968 163
Fragments arranged by size:
968 354 163 9
TatI w'GTAC_w
Cuts at: 0 551 746 1494
Size: 551 195 748
Fragments arranged by size:
748 551 195
XmnI GAAnn'nnTTC
Cuts at: 0 995 1494
Size: 995 499
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AflIII AloI AlwNI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrFI BsrGI BssSI BstAPI BstYI BstZ17I
BtgI BtgZI DraIII EaeI EciI Eco57MI EcoO109I FspI
HaeII Hin4I HindIII MmeI MscI MslI MspA1I NaeI
NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI
PshAI PstI PvuII SanDI SexAI SfcI SmlI StuI
StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AgeI AhdI AleI AlfI ApaI
ApaLI ApoI AscI AseI AsiSI BaeI BbeI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BsaI BsaWI
BsgI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BssHII
BstBI BstEII BstXI Bsu36I BtsI ClaI CspCI DraI
DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspAI HincII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI