Restriction map for
pFN21AE5649
(Linear) (Six-base) MAPSORT of: KIEE5649.seq Check: 7005 from: 1 to: 1482
KIEE5649.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 695 1482
Size: 695 787
AfeI AGC'GCT
Cuts at: 0 190 1482
Size: 190 1292
AflIII A'CryG_T
Cuts at: 0 1415 1428 1482
Size: 1415 13 54
Fragments arranged by size:
1415 54 13
AleI CACnn'nnGTG
Cuts at: 0 1168 1482
Size: 1168 314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 943 1482
Size: 943 539
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 574 606 1482
Size: 574 32 876
Fragments arranged by size:
876 574 32
AlwNI CAG_nnn'CTG
Cuts at: 0 720 1482
Size: 720 762
ApaI G_GGCC'C
Cuts at: 0 224 1482
Size: 224 1258
BamHI G'GATC_C
Cuts at: 0 777 1482
Size: 777 705
BanI G'GyrC_C
Cuts at: 0 493 904 1162 1482
Size: 493 411 258 320
Fragments arranged by size:
493 411 320 258
BanII G_rGCy'C
Cuts at: 0 224 1189 1346 1482
Size: 224 965 157 136
Fragments arranged by size:
965 224 157 136
BbeI G_GCGC'C
Cuts at: 0 497 1482
Size: 497 985
BbsI GAAGACnn'nnnn_
Cuts at: 0 321 1482
Size: 321 1161
BbvCI CC'TCA_GC
Cuts at: 0 284 1482
Size: 284 1198
Bme1580I G_kGCm'C
Cuts at: 0 224 487 907 1165 1292 1482
Size: 224 263 420 258 127 190
Fragments arranged by size:
420 263 258 224 190 127
BmgBI CAC'GTC
Cuts at: 0 635 1092 1416 1482
Size: 635 457 324 66
Fragments arranged by size:
635 457 324 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 333 1199 1389 1482
Size: 333 866 190 93
Fragments arranged by size:
866 333 190 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 684 1482
Size: 684 798
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1482
Size: 751 731
Bpu10I CC'TnA_GC
Cuts at: 0 284 520 1482
Size: 284 236 962
Fragments arranged by size:
962 284 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1369 1482
Size: 1369 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 904 1193 1482
Size: 904 289 289
Fragments arranged by size:
904 289 289
BsaAI yAC'GTr
Cuts at: 0 1431 1482
Size: 1431 51
BsaHI Gr'CG_yC
Cuts at: 0 473 494 1482
Size: 473 21 988
Fragments arranged by size:
988 473 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 301 451 466 844 847 1000 1482
Size: 301 150 15 378 3 153 482
Fragments arranged by size:
482 378 301 153 150 15 3
BseYI C'CCAG_C
Cuts at: 0 1182 1238 1482
Size: 1182 56 244
Fragments arranged by size:
1182 244 56
BsiHKAI G_wGCw'C
Cuts at: 0 761 1346 1482
Size: 761 585 136
Fragments arranged by size:
761 585 136
BsiWI C'GTAC_G
Cuts at: 0 769 1482
Size: 769 713
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1082 1482
Size: 1082 400
Bsp1286I G_dGCh'C
Cuts at: 0 224 487 761 907 1165 1189 1292 1346
Size: 224 263 274 146 258 24 103 54
Cuts at: 1346 1482
Size: 136
Fragments arranged by size:
274 263 258 224 146 136 103 54
24
BspHI T'CATG_A
Cuts at: 0 173 950 1482
Size: 173 777 532
Fragments arranged by size:
777 532 173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 146 695 1482
Size: 146 549 787
Fragments arranged by size:
787 549 146
BsrDI GCAATG_nn'
Cuts at: 0 115 1482
Size: 115 1367
BsrFI r'CCGG_y
Cuts at: 0 1013 1482
Size: 1013 469
BsrGI T'GTAC_A
Cuts at: 0 161 1482
Size: 161 1321
BssHII G'CGCG_C
Cuts at: 0 366 1482
Size: 366 1116
BssSI C'ACGA_G
Cuts at: 0 1069 1482
Size: 1069 413
BstXI CCAn_nnnn'nTGG
Cuts at: 0 216 753 1084 1107 1431 1482
Size: 216 537 331 23 324 51
Fragments arranged by size:
537 331 324 216 51 23
BstYI r'GATC_y
Cuts at: 0 777 1073 1482
Size: 777 296 409
Fragments arranged by size:
777 409 296
Bsu36I CC'TnA_GG
Cuts at: 0 9 1157 1403 1482
Size: 9 1148 246 79
Fragments arranged by size:
1148 246 79 9
BtgI C'CryG_G
Cuts at: 0 644 790 1482
Size: 644 146 692
Fragments arranged by size:
692 644 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 378 1482
Size: 378 1104
BtsI GCAGTG_nn'
Cuts at: 0 1067 1482
Size: 1067 415
ClaI AT'CG_AT
Cuts at: 0 503 1482
Size: 503 979
EaeI y'GGCC_r
Cuts at: 0 1331 1482
Size: 1331 151
EarI CTCTTCn'nnn_
Cuts at: 0 76 675 898 1267 1357 1482
Size: 76 599 223 369 90 125
Fragments arranged by size:
599 369 223 125 90 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1369 1482
Size: 1369 113
EcoICRI GAG'CTC
Cuts at: 0 1344 1482
Size: 1344 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1482
Size: 751 731
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1156 1482
Size: 1156 326
EcoO109I rG'GnC_Cy
Cuts at: 0 106 220 221 1482
Size: 106 114 1 1261
Fragments arranged by size:
1261 114 106 1
FspI TGC'GCA
Cuts at: 0 607 1482
Size: 607 875
HaeII r_GCGC'y
Cuts at: 0 192 387 497 1482
Size: 192 195 110 985
Fragments arranged by size:
985 195 192 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 1079 1111 1482
Size: 580 32 467 32 371
Fragments arranged by size:
580 467 371 32 32
HincII GTy'rAC
Cuts at: 0 638 1482
Size: 638 844
KasI G'GCGC_C
Cuts at: 0 493 1482
Size: 493 989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1241 1482
Size: 1241 241
MscI TGG'CCA
Cuts at: 0 1333 1482
Size: 1333 149
MslI CAynn'nnrTG
Cuts at: 0 820 1168 1366 1414 1482
Size: 820 348 198 48 68
Fragments arranged by size:
820 348 198 68 48
MspA1I CmG'CkG
Cuts at: 0 296 1482
Size: 296 1186
NarI GG'CG_CC
Cuts at: 0 494 1482
Size: 494 988
NspI r_CATG'y
Cuts at: 0 825 1482
Size: 825 657
PfoI T'CCnGG_A
Cuts at: 0 102 446 1482
Size: 102 344 1036
Fragments arranged by size:
1036 344 102
PmlI CAC'GTG
Cuts at: 0 1431 1482
Size: 1431 51
PpuMI rG'GwC_Cy
Cuts at: 0 106 1482
Size: 106 1376
PspOMI G'GGCC_C
Cuts at: 0 220 1482
Size: 220 1262
SacI G_AGCT'C
Cuts at: 0 1346 1482
Size: 1346 136
SanDI GG'GwC_CC
Cuts at: 0 106 1482
Size: 106 1376
SapI GCTCTTCn'nnn_
Cuts at: 0 76 1482
Size: 76 1406
SexAI A'CCwGG_T
Cuts at: 0 869 1482
Size: 869 613
SfoI GGC'GCC
Cuts at: 0 495 1482
Size: 495 987
SmlI C'TyrA_G
Cuts at: 0 1384 1482
Size: 1384 98
SphI G_CATG'C
Cuts at: 0 825 1482
Size: 825 657
StuI AGG'CCT
Cuts at: 0 709 1328 1482
Size: 709 619 154
Fragments arranged by size:
709 619 154
StyI C'CwwG_G
Cuts at: 0 974 1482
Size: 974 508
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 974 1482
Size: 974 508
TatI w'GTAC_w
Cuts at: 0 161 1482
Size: 161 1321
Tth111I GACn'n_nGTC
Cuts at: 0 325 1482
Size: 325 1157
XmnI GAAnn'nnTTC
Cuts at: 0 892 1315 1482
Size: 892 423 167
Fragments arranged by size:
892 423 167
Enzymes that do cut:
AarI AfeI AflIII AleI AlfI AloI AlwNI ApaI
BamHI BanI BanII BbeI BbsI BbvCI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BseRI BseYI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NarI NspI PfoI PmlI
PpuMI PspOMI SacI SanDI SapI SexAI SfoI SmlI
SphI StuI StyI TaqII TatI Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI
BsaBI BsaWI BsaXI BsgI BsiEI BsmI BspEI BsrBI
BstAPI BstBI BstEII BstZ17I CspCI DraI DraIII DrdI
EagI EcoRI EcoRV FalI FseI FspAI HindIII HpaI
KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TstI
XbaI XcmI XhoI XmaI ZraI