Restriction map for
pFN21AE5647
(Linear) (Six-base) MAPSORT of: KIEE5647.seq Check: 4103 from: 1 to: 1470
KIEE5647.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
AclI AA'CG_TT
Cuts at: 0 555 707 1470
Size: 555 152 763
Fragments arranged by size:
763 555 152
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1470
Size: 961 509
AflIII A'CryG_T
Cuts at: 0 984 1470
Size: 984 486
AlwNI CAG_nnn'CTG
Cuts at: 0 632 1048 1470
Size: 632 416 422
Fragments arranged by size:
632 422 416
ApaLI G'TGCA_C
Cuts at: 0 1054 1470
Size: 1054 416
ApoI r'AATT_y
Cuts at: 0 396 841 1123 1198 1470
Size: 396 445 282 75 272
Fragments arranged by size:
445 396 282 272 75
AseI AT'TA_AT
Cuts at: 0 1162 1470
Size: 1162 308
AvaI C'yCGr_G
Cuts at: 0 678 1470
Size: 678 792
BamHI G'GATC_C
Cuts at: 0 635 1470
Size: 635 835
BanII G_rGCy'C
Cuts at: 0 70 1009 1470
Size: 70 939 461
Fragments arranged by size:
939 461 70
BglII A'GATC_T
Cuts at: 0 641 1470
Size: 641 829
Bme1580I G_kGCm'C
Cuts at: 0 1058 1153 1470
Size: 1058 95 317
Fragments arranged by size:
1058 317 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 103 1470
Size: 103 1367
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 100 941 1470
Size: 100 841 529
Fragments arranged by size:
841 529 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1394 1470
Size: 78 1316 76
Fragments arranged by size:
1316 78 76
BsaBI GATnn'nnATC
Cuts at: 0 640 1470
Size: 640 830
BsaWI w'CCGG_w
Cuts at: 0 1001 1470
Size: 1001 469
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 92 289 429 475 1470
Size: 92 197 140 46 995
Fragments arranged by size:
995 197 140 92 46
BseYI C'CCAG_C
Cuts at: 0 889 1470
Size: 889 581
BsiHKAI G_wGCw'C
Cuts at: 0 26 70 519 1058 1470
Size: 26 44 449 539 412
Fragments arranged by size:
539 449 412 44 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 892 1470
Size: 892 578
Bsp1286I G_dGCh'C
Cuts at: 0 26 70 519 1009 1058 1153 1470
Size: 26 44 449 490 49 95 317
Fragments arranged by size:
490 449 317 95 49 44 26
BspHI T'CATG_A
Cuts at: 0 383 1470
Size: 383 1087
BsrDI GCAATG_nn'
Cuts at: 0 351 531 1470
Size: 351 180 939
Fragments arranged by size:
939 351 180
BsrFI r'CCGG_y
Cuts at: 0 1321 1470
Size: 1321 149
BssSI C'ACGA_G
Cuts at: 0 1057 1470
Size: 1057 413
BstYI r'GATC_y
Cuts at: 0 365 635 641 1470
Size: 365 270 6 829
Fragments arranged by size:
829 365 270 6
Bsu36I CC'TnA_GG
Cuts at: 0 779 1470
Size: 779 691
BtsI GCAGTG_nn'
Cuts at: 0 251 866 1112 1470
Size: 251 615 246 358
Fragments arranged by size:
615 358 251 246
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1395 1430 1470
Size: 1395 35 40
Fragments arranged by size:
1395 40 35
DraIII CAC_nnn'GTG
Cuts at: 0 490 1470
Size: 490 980
EaeI y'GGCC_r
Cuts at: 0 1319 1470
Size: 1319 151
EarI CTCTTCn'nnn_
Cuts at: 0 282 406 968 1470
Size: 282 124 562 502
Fragments arranged by size:
562 502 282 124
EcoICRI GAG'CTC
Cuts at: 0 68 1470
Size: 68 1402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 961 1394 1470
Size: 78 883 433 76
Fragments arranged by size:
883 433 78 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 457 1470
Size: 457 1013
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 911 943 1470
Size: 911 32 527
Fragments arranged by size:
911 527 32
HincII GTy'rAC
Cuts at: 0 876 1386 1470
Size: 876 510 84
Fragments arranged by size:
876 510 84
HindIII A'AGCT_T
Cuts at: 0 855 1470
Size: 855 615
MslI CAynn'nnrTG
Cuts at: 0 223 236 1470
Size: 223 13 1234
Fragments arranged by size:
1234 223 13
MspA1I CmG'CkG
Cuts at: 0 449 1452 1470
Size: 449 1003 18
Fragments arranged by size:
1003 449 18
NaeI GCC'GGC
Cuts at: 0 1323 1470
Size: 1323 147
NgoMIV G'CCGG_C
Cuts at: 0 1321 1470
Size: 1321 149
NspI r_CATG'y
Cuts at: 0 1017 1035 1470
Size: 1017 18 435
Fragments arranged by size:
1017 435 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 115 1470
Size: 115 1355
PfoI T'CCnGG_A
Cuts at: 0 968 1470
Size: 968 502
PsiI TTA'TAA
Cuts at: 0 566 1470
Size: 566 904
PstI C_TGCA'G
Cuts at: 0 873 1470
Size: 873 597
PvuII CAG'CTG
Cuts at: 0 1452 1470
Size: 1452 18
SacI G_AGCT'C
Cuts at: 0 70 1470
Size: 70 1400
SfcI C'TryA_G
Cuts at: 0 127 869 1470
Size: 127 742 601
Fragments arranged by size:
742 601 127
SmaI CCC'GGG
Cuts at: 0 680 1470
Size: 680 790
SphI G_CATG'C
Cuts at: 0 1017 1470
Size: 1017 453
StuI AGG'CCT
Cuts at: 0 481 1470
Size: 481 989
StyI C'CwwG_G
Cuts at: 0 476 1145 1470
Size: 476 669 325
Fragments arranged by size:
669 476 325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 962 1470
Size: 962 508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 504 1470
Size: 504 966
XmaI C'CCGG_G
Cuts at: 0 678 1470
Size: 678 792
Enzymes that do cut:
AclI AcuI AflIII AlwNI ApaLI ApoI AseI AvaI
BamHI BanII BglII Bme1580I BmrI BplI BpmI BsaBI
BsaWI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BsrDI
BsrFI BssSI BstYI Bsu36I BtsI CspCI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI Hin4I HincII HindIII MslI
MspA1I NaeI NgoMIV NspI PflMI PfoI PsiI PstI
PvuII SacI SfcI SmaI SphI StuI StyI TaqII
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AsiSI AvrII BaeI
BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BsgI BsiEI BsiWI BsmI BspEI BspMI
BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
BtgI BtgZI ClaI DraI DrdI EagI EciI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI MmeI MscI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI
XmnI ZraI