Restriction map for
pFN21AE5643
(Linear) (Six-base) MAPSORT of: KIEE5643.seq Check: 7388 from: 1 to: 1416
KIEE5643.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 846 1416
Size: 846 570
AflIII A'CryG_T
Cuts at: 0 763 1416
Size: 763 653
AlwNI CAG_nnn'CTG
Cuts at: 0 72 1416
Size: 72 1344
ApoI r'AATT_y
Cuts at: 0 481 812 1064 1403 1416
Size: 481 331 252 339 13
Fragments arranged by size:
481 339 331 252 13
AseI AT'TA_AT
Cuts at: 0 800 1416
Size: 800 616
BanI G'GyrC_C
Cuts at: 0 1391 1416
Size: 1391 25
BciVI GTATCCnnnnn_n'
Cuts at: 0 442 1416
Size: 442 974
BfrBI ATG'CAT
Cuts at: 0 732 1416
Size: 732 684
BglII A'GATC_T
Cuts at: 0 960 1416
Size: 960 456
BmrI ACTGGGnnnn_n'
Cuts at: 0 394 1082 1166 1416
Size: 394 688 84 250
Fragments arranged by size:
688 394 250 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1395 1416
Size: 133 1262 21
Fragments arranged by size:
1262 133 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 1416
Size: 195 1221
BsaBI GATnn'nnATC
Cuts at: 0 635 1416
Size: 635 781
BsaWI w'CCGG_w
Cuts at: 0 1286 1416
Size: 1286 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 1416
Size: 187 30 1199
Fragments arranged by size:
1199 187 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 573 1416
Size: 573 843
BseYI C'CCAG_C
Cuts at: 0 222 1416
Size: 222 1194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1416
Size: 257 1159
BsmI GAATG_Cn'
Cuts at: 0 607 1357 1416
Size: 607 750 59
Fragments arranged by size:
750 607 59
BspEI T'CCGG_A
Cuts at: 0 1286 1416
Size: 1286 130
BspHI T'CATG_A
Cuts at: 0 804 1056 1416
Size: 804 252 360
Fragments arranged by size:
804 360 252
BsrGI T'GTAC_A
Cuts at: 0 95 420 1416
Size: 95 325 996
Fragments arranged by size:
996 325 95
BstYI r'GATC_y
Cuts at: 0 364 960 1416
Size: 364 596 456
Fragments arranged by size:
596 456 364
Bsu36I CC'TnA_GG
Cuts at: 0 556 1416
Size: 556 860
BtgI C'CryG_G
Cuts at: 0 199 1416
Size: 199 1217
ClaI AT'CG_AT
Cuts at: 0 1052 1416
Size: 1052 364
EaeI y'GGCC_r
Cuts at: 0 231 1416
Size: 231 1185
EarI CTCTTCn'nnn_
Cuts at: 0 12 206 597 1072 1416
Size: 12 194 391 475 344
Fragments arranged by size:
475 391 344 194 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 846 1395 1416
Size: 133 713 549 21
Fragments arranged by size:
713 549 133 21
EcoO109I rG'GnC_Cy
Cuts at: 0 662 1250 1416
Size: 662 588 166
Fragments arranged by size:
662 588 166
EcoRI G'AATT_C
Cuts at: 0 1403 1416
Size: 1403 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 385 417 1416
Size: 385 32 999
Fragments arranged by size:
999 385 32
HindIII A'AGCT_T
Cuts at: 0 625 1416
Size: 625 791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 185 1416
Size: 161 24 1231
Fragments arranged by size:
1231 161 24
MslI CAynn'nnrTG
Cuts at: 0 596 1416
Size: 596 820
MspA1I CmG'CkG
Cuts at: 0 69 1416
Size: 69 1347
NcoI C'CATG_G
Cuts at: 0 199 1416
Size: 199 1217
NdeI CA'TA_TG
Cuts at: 0 1015 1416
Size: 1015 401
NsiI A_TGCA'T
Cuts at: 0 734 1416
Size: 734 682
NspI r_CATG'y
Cuts at: 0 568 767 1416
Size: 568 199 649
Fragments arranged by size:
649 568 199
PasI CC'CwG_GG
Cuts at: 0 12 54 1416
Size: 12 42 1362
Fragments arranged by size:
1362 42 12
PciI A'CATG_T
Cuts at: 0 763 1416
Size: 763 653
PfoI T'CCnGG_A
Cuts at: 0 781 1416
Size: 781 635
PpuMI rG'GwC_Cy
Cuts at: 0 1250 1416
Size: 1250 166
SapI GCTCTTCn'nnn_
Cuts at: 0 597 1416
Size: 597 819
SfcI C'TryA_G
Cuts at: 0 513 1254 1416
Size: 513 741 162
Fragments arranged by size:
741 513 162
SmlI C'TyrA_G
Cuts at: 0 174 1416
Size: 174 1242
SphI G_CATG'C
Cuts at: 0 568 1416
Size: 568 848
StuI AGG'CCT
Cuts at: 0 1027 1416
Size: 1027 389
StyI C'CwwG_G
Cuts at: 0 199 1416
Size: 199 1217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 1416
Size: 67 1349
TatI w'GTAC_w
Cuts at: 0 95 420 1416
Size: 95 325 996
Fragments arranged by size:
996 325 95
XmnI GAAnn'nnTTC
Cuts at: 0 859 1416
Size: 859 557
Enzymes that do cut:
AcuI AflIII AlwNI ApoI AseI BanI BciVI BfrBI
BglII BmrI BpmI BpuEI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsmI BspEI BspHI BsrGI BstYI Bsu36I
BtgI ClaI EaeI EarI Eco57MI EcoO109I EcoRI Hin4I
HindIII MmeI MslI MspA1I NcoI NdeI NsiI NspI
PasI PciI PfoI PpuMI SapI SfcI SmlI SphI
StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbsI BbvCI
BcgI BclI BglI BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaHI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgZI BtsI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NgoMIV NheI NotI
NruI PacI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI