Restriction map for
pFN21AE5642
(Linear) (Six-base) MAPSORT of: KIEE5642.seq Check: 1852 from: 1 to: 1509
KIEE5642.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
AatII G_ACGT'C
Cuts at: 0 524 1509
Size: 524 985
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1363 1509
Size: 799 564 146
Fragments arranged by size:
799 564 146
AlwNI CAG_nnn'CTG
Cuts at: 0 259 990 1231 1322 1509
Size: 259 731 241 91 187
Fragments arranged by size:
731 259 241 187 91
ApaI G_GGCC'C
Cuts at: 0 122 1509
Size: 122 1387
ApoI r'AATT_y
Cuts at: 0 110 738 837 1509
Size: 110 628 99 672
Fragments arranged by size:
672 628 110 99
BamHI G'GATC_C
Cuts at: 0 648 1509
Size: 648 861
BanI G'GyrC_C
Cuts at: 0 1026 1492 1509
Size: 1026 466 17
Fragments arranged by size:
1026 466 17
BanII G_rGCy'C
Cuts at: 0 122 884 1509
Size: 122 762 625
Fragments arranged by size:
762 625 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 294 1509
Size: 294 1215
BbvCI CC'TCA_GC
Cuts at: 0 362 1509
Size: 362 1147
BclI T'GATC_A
Cuts at: 0 266 548 1509
Size: 266 282 961
Fragments arranged by size:
961 282 266
Bme1580I G_kGCm'C
Cuts at: 0 122 1509
Size: 122 1387
BmrI ACTGGGnnnn_n'
Cuts at: 0 444 1509
Size: 444 1065
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1358 1509
Size: 1358 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1509
Size: 898 611
Bpu10I CC'TnA_GC
Cuts at: 0 362 1434 1509
Size: 362 1072 75
Fragments arranged by size:
1072 362 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1138 1509
Size: 409 729 371
Fragments arranged by size:
729 409 371
BsaBI GATnn'nnATC
Cuts at: 0 387 1509
Size: 387 1122
BsaHI Gr'CG_yC
Cuts at: 0 521 1509
Size: 521 988
BsaWI w'CCGG_w
Cuts at: 0 311 1509
Size: 311 1198
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 1509
Size: 52 1457
BsiHKAI G_wGCw'C
Cuts at: 0 62 884 1509
Size: 62 822 625
Fragments arranged by size:
822 625 62
Bsp1286I G_dGCh'C
Cuts at: 0 62 122 884 1509
Size: 62 60 762 625
Fragments arranged by size:
762 625 62 60
BspHI T'CATG_A
Cuts at: 0 1346 1509
Size: 1346 163
BsrDI GCAATG_nn'
Cuts at: 0 391 443 1509
Size: 391 52 1066
Fragments arranged by size:
1066 391 52
BssSI C'ACGA_G
Cuts at: 0 883 927 1509
Size: 883 44 582
Fragments arranged by size:
883 582 44
BstAPI GCAn_nnn'nTGC
Cuts at: 0 990 1509
Size: 990 519
BstXI CCAn_nnnn'nTGG
Cuts at: 0 88 1173 1220 1509
Size: 88 1085 47 289
Fragments arranged by size:
1085 289 88 47
BstYI r'GATC_y
Cuts at: 0 255 648 1281 1415 1509
Size: 255 393 633 134 94
Fragments arranged by size:
633 393 255 134 94
BtgI C'CryG_G
Cuts at: 0 23 1509
Size: 23 1486
BtsI GCAGTG_nn'
Cuts at: 0 939 1509
Size: 939 570
DraIII CAC_nnn'GTG
Cuts at: 0 804 1509
Size: 804 705
EaeI y'GGCC_r
Cuts at: 0 20 962 1509
Size: 20 942 547
Fragments arranged by size:
942 547 20
EarI CTCTTCn'nnn_
Cuts at: 0 373 1205 1509
Size: 373 832 304
Fragments arranged by size:
832 373 304
EcoICRI GAG'CTC
Cuts at: 0 882 1509
Size: 882 627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 898 1363 1509
Size: 799 99 465 146
Fragments arranged by size:
799 465 146 99
EcoO109I rG'GnC_Cy
Cuts at: 0 118 530 1509
Size: 118 412 979
Fragments arranged by size:
979 412 118
EcoRI G'AATT_C
Cuts at: 0 110 837 1509
Size: 110 727 672
Fragments arranged by size:
727 672 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 560 592 816 848 1047 1079 1509
Size: 560 32 224 32 199 32 430
Fragments arranged by size:
560 430 224 199 32 32 32
HincII GTy'rAC
Cuts at: 0 232 1509
Size: 232 1277
HindIII A'AGCT_T
Cuts at: 0 103 625 1431 1509
Size: 103 522 806 78
Fragments arranged by size:
806 522 103 78
MfeI C'AATT_G
Cuts at: 0 1103 1509
Size: 1103 406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1509
Size: 427 1082
MscI TGG'CCA
Cuts at: 0 22 964 1509
Size: 22 942 545
Fragments arranged by size:
942 545 22
MslI CAynn'nnrTG
Cuts at: 0 86 544 974 1075 1330 1351 1509
Size: 86 458 430 101 255 21 158
Fragments arranged by size:
458 430 255 158 101 86 21
MspA1I CmG'CkG
Cuts at: 0 819 1509
Size: 819 690
NcoI C'CATG_G
Cuts at: 0 23 1509
Size: 23 1486
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 565 597 1509
Size: 565 32 912
Fragments arranged by size:
912 565 32
PspOMI G'GGCC_C
Cuts at: 0 118 1509
Size: 118 1391
PstI C_TGCA'G
Cuts at: 0 995 1509
Size: 995 514
PvuII CAG'CTG
Cuts at: 0 819 1509
Size: 819 690
SacI G_AGCT'C
Cuts at: 0 884 1509
Size: 884 625
SapI GCTCTTCn'nnn_
Cuts at: 0 1205 1509
Size: 1205 304
ScaI AGT'ACT
Cuts at: 0 1219 1509
Size: 1219 290
SfcI C'TryA_G
Cuts at: 0 356 534 991 1051 1509
Size: 356 178 457 60 458
Fragments arranged by size:
458 457 356 178 60
SmlI C'TyrA_G
Cuts at: 0 424 1117 1509
Size: 424 693 392
Fragments arranged by size:
693 424 392
SspI AAT'ATT
Cuts at: 0 147 1509
Size: 147 1362
StuI AGG'CCT
Cuts at: 0 318 1509
Size: 318 1191
StyI C'CwwG_G
Cuts at: 0 23 1509
Size: 23 1486
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1047 1509
Size: 1047 462
TatI w'GTAC_w
Cuts at: 0 1217 1509
Size: 1217 292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1173 1509
Size: 1173 336
XmnI GAAnn'nnTTC
Cuts at: 0 81 1509
Size: 81 1428
ZraI GAC'GTC
Cuts at: 0 522 1509
Size: 522 987
Enzymes that do cut:
AatII AcuI AlwNI ApaI ApoI BamHI BanI BanII
BbsI BbvCI BclI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaBI BsaHI BsaWI BseRI BsiHKAI Bsp1286I BspHI
BsrDI BssSI BstAPI BstXI BstYI BtgI BtsI DraIII
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NcoI PpiI
PspOMI PstI PvuII SacI SapI ScaI SfcI SmlI
SspI StuI StyI TaqII TatI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmtI BsaI BsaAI BsaXI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TstI
Tth111I XbaI XhoI XmaI