Restriction map for
pFN21AE5641
(Linear) (Six-base) MAPSORT of: KIEE5641.seq Check: 1959 from: 1 to: 1749
KIEE5641.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
Acc65I G'GTAC_C
Cuts at: 0 1537 1749
Size: 1537 212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 514 607 1620 1749
Size: 337 177 93 1013 129
Fragments arranged by size:
1013 337 177 129 93
AflIII A'CryG_T
Cuts at: 0 342 431 804 1749
Size: 342 89 373 945
Fragments arranged by size:
945 373 342 89
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1094 1749
Size: 1094 655
AlwNI CAG_nnn'CTG
Cuts at: 0 360 645 1446 1749
Size: 360 285 801 303
Fragments arranged by size:
801 360 303 285
ApoI r'AATT_y
Cuts at: 0 253 708 1313 1749
Size: 253 455 605 436
Fragments arranged by size:
605 455 436 253
BamHI G'GATC_C
Cuts at: 0 1238 1749
Size: 1238 511
BanI G'GyrC_C
Cuts at: 0 3 540 1537 1585 1749
Size: 3 537 997 48 164
Fragments arranged by size:
997 537 164 48 3
BbeI G_GCGC'C
Cuts at: 0 7 1749
Size: 7 1742
BbsI GAAGACnn'nnnn_
Cuts at: 0 652 899 1749
Size: 652 247 850
Fragments arranged by size:
850 652 247
BbvCI CC'TCA_GC
Cuts at: 0 1395 1749
Size: 1395 354
BciVI GTATCCnnnnn_n'
Cuts at: 0 1722 1749
Size: 1722 27
Bme1580I G_kGCm'C
Cuts at: 0 543 563 1489 1749
Size: 543 20 926 260
Fragments arranged by size:
926 543 260 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 801 1749
Size: 801 948
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1594 1705 1749
Size: 926 668 111 44
Fragments arranged by size:
926 668 111 44
Bpu10I CC'TnA_GC
Cuts at: 0 1395 1490 1652 1749
Size: 1395 95 162 97
Fragments arranged by size:
1395 162 97 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1386 1440 1749
Size: 1386 54 309
Fragments arranged by size:
1386 309 54
BsaHI Gr'CG_yC
Cuts at: 0 4 1749
Size: 4 1745
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1188 1218 1749
Size: 1188 30 531
Fragments arranged by size:
1188 531 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 40 571 1591 1749
Size: 37 3 531 1020 158
Fragments arranged by size:
1020 531 158 37 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1538 1749
Size: 1538 211
BsiHKAI G_wGCw'C
Cuts at: 0 146 1470 1749
Size: 146 1324 279
Fragments arranged by size:
1324 279 146
BsmI GAATG_Cn'
Cuts at: 0 1083 1749
Size: 1083 666
Bsp1286I G_dGCh'C
Cuts at: 0 146 543 563 1470 1489 1749
Size: 146 397 20 907 19 260
Fragments arranged by size:
907 397 260 146 20 19
BspHI T'CATG_A
Cuts at: 0 216 1166 1749
Size: 216 950 583
Fragments arranged by size:
950 583 216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 497 1736 1749
Size: 497 1239 13
Fragments arranged by size:
1239 497 13
BsrDI GCAATG_nn'
Cuts at: 0 293 1509 1749
Size: 293 1216 240
Fragments arranged by size:
1216 293 240
BstAPI GCAn_nnn'nTGC
Cuts at: 0 645 1749
Size: 645 1104
BstEII G'GTnAC_C
Cuts at: 0 1347 1749
Size: 1347 402
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1056 1749
Size: 1056 693
BstYI r'GATC_y
Cuts at: 0 239 465 1238 1749
Size: 239 226 773 511
Fragments arranged by size:
773 511 239 226
BtgI C'CryG_G
Cuts at: 0 1736 1749
Size: 1736 13
BtsI GCAGTG_nn'
Cuts at: 0 1489 1521 1749
Size: 1489 32 228
Fragments arranged by size:
1489 228 32
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1161 1196 1749
Size: 1161 35 553
Fragments arranged by size:
1161 553 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1384 1749
Size: 1384 365
EaeI y'GGCC_r
Cuts at: 0 735 1020 1206 1372 1719 1733 1749
Size: 735 285 186 166 347 14 16
Fragments arranged by size:
735 347 285 186 166 16 14
EarI CTCTTCn'nnn_
Cuts at: 0 47 233 1749
Size: 47 186 1516
Fragments arranged by size:
1516 186 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 514 607 926 1594 1620 1705 1749
Size: 337 177 93 319 668 26 85 44
Fragments arranged by size:
668 337 319 177 93 85 44 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 493 586 724 1749
Size: 493 93 138 1025
Fragments arranged by size:
1025 493 138 93
EcoO109I rG'GnC_Cy
Cuts at: 0 276 814 1099 1622 1631 1749
Size: 276 538 285 523 9 118
Fragments arranged by size:
538 523 285 276 118 9
HaeII r_GCGC'y
Cuts at: 0 7 1749
Size: 7 1742
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 781 813 1749
Size: 781 32 936
Fragments arranged by size:
936 781 32
KasI G'GCGC_C
Cuts at: 0 3 1749
Size: 3 1746
KpnI G_GTAC'C
Cuts at: 0 1541 1749
Size: 1541 208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 1749
Size: 363 1386
MscI TGG'CCA
Cuts at: 0 1022 1208 1374 1721 1749
Size: 1022 186 166 347 28
Fragments arranged by size:
1022 347 186 166 28
MslI CAynn'nnrTG
Cuts at: 0 546 1514 1749
Size: 546 968 235
Fragments arranged by size:
968 546 235
MspA1I CmG'CkG
Cuts at: 0 93 1572 1749
Size: 93 1479 177
Fragments arranged by size:
1479 177 93
NarI GG'CG_CC
Cuts at: 0 4 1749
Size: 4 1745
NdeI CA'TA_TG
Cuts at: 0 547 1749
Size: 547 1202
NspI r_CATG'y
Cuts at: 0 346 435 808 1749
Size: 346 89 373 941
Fragments arranged by size:
941 373 346 89
PasI CC'CwG_GG
Cuts at: 0 650 1749
Size: 650 1099
PciI A'CATG_T
Cuts at: 0 342 431 804 1749
Size: 342 89 373 945
Fragments arranged by size:
945 373 342 89
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 840 1381 1749
Size: 345 495 541 368
Fragments arranged by size:
541 495 368 345
PfoI T'CCnGG_A
Cuts at: 0 599 942 1682 1749
Size: 599 343 740 67
Fragments arranged by size:
740 599 343 67
PpuMI rG'GwC_Cy
Cuts at: 0 276 814 1099 1622 1749
Size: 276 538 285 523 127
Fragments arranged by size:
538 523 285 276 127
PshAI GACnn'nnGTC
Cuts at: 0 899 1749
Size: 899 850
PsiI TTA'TAA
Cuts at: 0 1295 1749
Size: 1295 454
PvuII CAG'CTG
Cuts at: 0 1572 1749
Size: 1572 177
SanDI GG'GwC_CC
Cuts at: 0 276 1749
Size: 276 1473
ScaI AGT'ACT
Cuts at: 0 1213 1660 1749
Size: 1213 447 89
Fragments arranged by size:
1213 447 89
SfcI C'TryA_G
Cuts at: 0 171 1749
Size: 171 1578
SfoI GGC'GCC
Cuts at: 0 5 1749
Size: 5 1744
SmlI C'TyrA_G
Cuts at: 0 1365 1419 1749
Size: 1365 54 330
Fragments arranged by size:
1365 330 54
SspI AAT'ATT
Cuts at: 0 617 1749
Size: 617 1132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 558 664 755 1512 1749
Size: 558 106 91 757 237
Fragments arranged by size:
757 558 237 106 91
TatI w'GTAC_w
Cuts at: 0 77 801 1211 1658 1749
Size: 77 724 410 447 91
Fragments arranged by size:
724 447 410 91 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1083 1115 1188 1220 1749
Size: 1083 32 73 32 529
Fragments arranged by size:
1083 529 73 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 595 1608 1749
Size: 595 1013 141
Fragments arranged by size:
1013 595 141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 837 1272 1749
Size: 837 435 477
Fragments arranged by size:
837 477 435
XmnI GAAnn'nnTTC
Cuts at: 0 227 896 1749
Size: 227 669 853
Fragments arranged by size:
853 669 227
Enzymes that do cut:
Acc65I AcuI AflIII AlfI AlwNI ApoI BamHI BanI
BbeI BbsI BbvCI BciVI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BstAPI BstEII BstXI BstYI BtgI
BtsI CspCI DrdI EaeI EarI Eco57MI EcoNI EcoO109I
HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I
NarI NdeI NspI PasI PciI PflMI PfoI PpuMI
PshAI PsiI PvuII SanDI ScaI SfcI SfoI SmlI
SspI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanII BcgI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI
BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI NaeI
NcoI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SapI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI StyI
SwaI XbaI XhoI XmaI ZraI