Restriction map for
pFN21AE5640
(Linear) (Six-base) MAPSORT of: KIEE5640.seq Check: 6546 from: 1 to: 1695
KIEE5640.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
Acc65I G'GTAC_C
Cuts at: 0 649 1695
Size: 649 1046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 883 1333 1475 1695
Size: 703 180 450 142 220
Fragments arranged by size:
703 450 220 180 142
AfeI AGC'GCT
Cuts at: 0 1318 1695
Size: 1318 377
AflIII A'CryG_T
Cuts at: 0 876 1695
Size: 876 819
AhdI GACnn_n'nnGTC
Cuts at: 0 966 1695
Size: 966 729
AleI CACnn'nnGTG
Cuts at: 0 571 1695
Size: 571 1124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 157 620 1695
Size: 157 463 1075
Fragments arranged by size:
1075 463 157
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 793 825 1695
Size: 793 32 870
Fragments arranged by size:
870 793 32
ApoI r'AATT_y
Cuts at: 0 864 1695
Size: 864 831
AvaI C'yCGr_G
Cuts at: 0 128 635 1695
Size: 128 507 1060
Fragments arranged by size:
1060 507 128
BamHI G'GATC_C
Cuts at: 0 1544 1695
Size: 1544 151
BanI G'GyrC_C
Cuts at: 0 649 1695
Size: 649 1046
BanII G_rGCy'C
Cuts at: 0 127 1537 1695
Size: 127 1410 158
Fragments arranged by size:
1410 158 127
BbsI GAAGACnn'nnnn_
Cuts at: 0 773 931 1030 1695
Size: 773 158 99 665
Fragments arranged by size:
773 665 158 99
BclI T'GATC_A
Cuts at: 0 1622 1695
Size: 1622 73
Bme1580I G_kGCm'C
Cuts at: 0 1009 1695
Size: 1009 686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 1579 1695
Size: 779 800 116
Fragments arranged by size:
800 779 116
Bpu10I CC'TnA_GC
Cuts at: 0 251 1695
Size: 251 1444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1695
Size: 493 1202
BsaAI yAC'GTr
Cuts at: 0 569 1551 1695
Size: 569 982 144
Fragments arranged by size:
982 569 144
BsaWI w'CCGG_w
Cuts at: 0 73 296 746 1541 1695
Size: 73 223 450 795 154
Fragments arranged by size:
795 450 223 154 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 941 971 1577 1607 1695
Size: 941 30 606 30 88
Fragments arranged by size:
941 606 88 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 315 731 1695
Size: 315 416 964
Fragments arranged by size:
964 416 315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 973 1695
Size: 973 722
BsiEI CG_ry'CG
Cuts at: 0 9 78 1695
Size: 9 69 1617
Fragments arranged by size:
1617 69 9
BsiHKAI G_wGCw'C
Cuts at: 0 127 1695
Size: 127 1568
BsmI GAATG_Cn'
Cuts at: 0 987 1695
Size: 987 708
Bsp1286I G_dGCh'C
Cuts at: 0 127 1009 1537 1695
Size: 127 882 528 158
Fragments arranged by size:
882 528 158 127
BspEI T'CCGG_A
Cuts at: 0 746 1695
Size: 746 949
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 668 1695
Size: 668 1027
BsrBI CCG'CTC
Cuts at: 0 105 255 1695
Size: 105 150 1440
Fragments arranged by size:
1440 150 105
BsrDI GCAATG_nn'
Cuts at: 0 1496 1695
Size: 1496 199
BsrFI r'CCGG_y
Cuts at: 0 8 165 1695
Size: 8 157 1530
Fragments arranged by size:
1530 157 8
BssHII G'CGCG_C
Cuts at: 0 173 175 1695
Size: 173 2 1520
Fragments arranged by size:
1520 173 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 829 1695
Size: 829 866
BstYI r'GATC_y
Cuts at: 0 1061 1434 1544 1695
Size: 1061 373 110 151
Fragments arranged by size:
1061 373 151 110
Bsu36I CC'TnA_GG
Cuts at: 0 113 1695
Size: 113 1582
BtsI GCAGTG_nn'
Cuts at: 0 309 1695
Size: 309 1386
ClaI AT'CG_AT
Cuts at: 0 545 1695
Size: 545 1150
EaeI y'GGCC_r
Cuts at: 0 6 276 1695
Size: 6 270 1419
Fragments arranged by size:
1419 270 6
EagI C'GGCC_G
Cuts at: 0 6 1695
Size: 6 1689
EarI CTCTTCn'nnn_
Cuts at: 0 1249 1651 1695
Size: 1249 402 44
Fragments arranged by size:
1249 402 44
EcoICRI GAG'CTC
Cuts at: 0 125 1695
Size: 125 1570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 779 883 1333 1475 1579 1695
Size: 703 76 104 450 142 104 116
Fragments arranged by size:
703 450 142 116 104 104 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1201 1695
Size: 1201 494
EcoO109I rG'GnC_Cy
Cuts at: 0 183 1695
Size: 183 1512
EcoRV GAT'ATC
Cuts at: 0 564 804 1695
Size: 564 240 891
Fragments arranged by size:
891 564 240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1323 1398 1695
Size: 1323 75 297
Fragments arranged by size:
1323 297 75
HaeII r_GCGC'y
Cuts at: 0 1320 1695
Size: 1320 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 226 258 952 984 1695
Size: 43 32 151 32 694 32 711
Fragments arranged by size:
711 694 151 43 32 32 32
HincII GTy'rAC
Cuts at: 0 960 1695
Size: 960 735
HindIII A'AGCT_T
Cuts at: 0 1075 1695
Size: 1075 620
KpnI G_GTAC'C
Cuts at: 0 653 1695
Size: 653 1042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1426 1695
Size: 1426 269
MslI CAynn'nnrTG
Cuts at: 0 392 571 1162 1695
Size: 392 179 591 533
Fragments arranged by size:
591 533 392 179
NaeI GCC'GGC
Cuts at: 0 167 1695
Size: 167 1528
NdeI CA'TA_TG
Cuts at: 0 642 1695
Size: 642 1053
NgoMIV G'CCGG_C
Cuts at: 0 165 1695
Size: 165 1530
NspI r_CATG'y
Cuts at: 0 880 1695
Size: 880 815
PciI A'CATG_T
Cuts at: 0 876 1695
Size: 876 819
PmlI CAC'GTG
Cuts at: 0 1551 1695
Size: 1551 144
PsiI TTA'TAA
Cuts at: 0 776 1695
Size: 776 919
SacI G_AGCT'C
Cuts at: 0 127 1695
Size: 127 1568
ScaI AGT'ACT
Cuts at: 0 424 1695
Size: 424 1271
SmaI CCC'GGG
Cuts at: 0 130 637 1695
Size: 130 507 1058
Fragments arranged by size:
1058 507 130
SmlI C'TyrA_G
Cuts at: 0 508 1695
Size: 508 1187
TatI w'GTAC_w
Cuts at: 0 422 909 1461 1470 1695
Size: 422 487 552 9 225
Fragments arranged by size:
552 487 422 225 9
XmaI C'CCGG_G
Cuts at: 0 128 635 1695
Size: 128 507 1060
Fragments arranged by size:
1060 507 128
XmnI GAAnn'nnTTC
Cuts at: 0 1318 1381 1695
Size: 1318 63 314
Fragments arranged by size:
1318 314 63
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AhdI AleI AlfI AloI
ApoI AvaI BamHI BanI BanII BbsI BclI Bme1580I
BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssHII BstXI BstYI Bsu36I BtsI ClaI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HincII HindIII KpnI MmeI MslI NaeI
NdeI NgoMIV NspI PciI PmlI PsiI SacI ScaI
SmaI SmlI TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AlwNI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI BsaI BsaBI BsaHI BseYI BsiWI BsmBI
BspHI BsrGI BssSI BstAPI BstBI BstEII BstZ17I BtgI
BtgZI CspCI DraI DraIII DrdI EciI EcoRI FseI
FspI FspAI HpaI KasI MfeI MluI MscI MspA1I
NarI NcoI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI ZraI