Restriction map for
pFN21AE5637
(Linear) (Six-base) MAPSORT of: KIEE5637.seq Check: 400 from: 1 to: 1512
KIEE5637.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
AccI GT'mk_AC
Cuts at: 0 260 1512
Size: 260 1252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1017 1423 1512
Size: 539 478 406 89
Fragments arranged by size:
539 478 406 89
AflIII A'CryG_T
Cuts at: 0 349 992 1334 1512
Size: 349 643 342 178
Fragments arranged by size:
643 349 342 178
AleI CACnn'nnGTG
Cuts at: 0 1421 1512
Size: 1421 91
AlwNI CAG_nnn'CTG
Cuts at: 0 790 1512
Size: 790 722
ApaI G_GGCC'C
Cuts at: 0 1030 1512
Size: 1030 482
ApaLI G'TGCA_C
Cuts at: 0 773 1512
Size: 773 739
ApoI r'AATT_y
Cuts at: 0 467 532 546 1512
Size: 467 65 14 966
Fragments arranged by size:
966 467 65 14
AseI AT'TA_AT
Cuts at: 0 1123 1512
Size: 1123 389
AvrII C'CTAG_G
Cuts at: 0 1402 1512
Size: 1402 110
BamHI G'GATC_C
Cuts at: 0 498 1398 1512
Size: 498 900 114
Fragments arranged by size:
900 498 114
BanI G'GyrC_C
Cuts at: 0 1174 1512
Size: 1174 338
BanII G_rGCy'C
Cuts at: 0 1030 1512
Size: 1030 482
BbsI GAAGACnn'nnnn_
Cuts at: 0 397 599 1512
Size: 397 202 913
Fragments arranged by size:
913 397 202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1208 1242 1512
Size: 1208 34 270
Fragments arranged by size:
1208 270 34
BclI T'GATC_A
Cuts at: 0 1149 1512
Size: 1149 363
BglII A'GATC_T
Cuts at: 0 1040 1512
Size: 1040 472
Bme1580I G_kGCm'C
Cuts at: 0 777 1030 1512
Size: 777 253 482
Fragments arranged by size:
777 482 253
BmtI G_CTAG'C
Cuts at: 0 364 1512
Size: 364 1148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 1512
Size: 287 1225
Bpu10I CC'TnA_GC
Cuts at: 0 698 1512
Size: 698 814
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 803 1512
Size: 428 375 709
Fragments arranged by size:
709 428 375
BsiHKAI G_wGCw'C
Cuts at: 0 777 1512
Size: 777 735
BsmI GAATG_Cn'
Cuts at: 0 90 456 1512
Size: 90 366 1056
Fragments arranged by size:
1056 366 90
Bsp1286I G_dGCh'C
Cuts at: 0 777 1030 1512
Size: 777 253 482
Fragments arranged by size:
777 482 253
BspHI T'CATG_A
Cuts at: 0 1016 1512
Size: 1016 496
BsrDI GCAATG_nn'
Cuts at: 0 60 509 1512
Size: 60 449 1003
Fragments arranged by size:
1003 449 60
BstAPI GCAn_nnn'nTGC
Cuts at: 0 781 790 1512
Size: 781 9 722
Fragments arranged by size:
781 722 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1395 1512
Size: 1395 117
BstYI r'GATC_y
Cuts at: 0 498 1040 1398 1512
Size: 498 542 358 114
Fragments arranged by size:
542 498 358 114
DraI TTT'AAA
Cuts at: 0 240 537 708 1512
Size: 240 297 171 804
Fragments arranged by size:
804 297 240 171
DraIII CAC_nnn'GTG
Cuts at: 0 781 1512
Size: 781 731
EaeI y'GGCC_r
Cuts at: 0 576 753 1385 1512
Size: 576 177 632 127
Fragments arranged by size:
632 576 177 127
EarI CTCTTCn'nnn_
Cuts at: 0 68 182 1512
Size: 68 114 1330
Fragments arranged by size:
1330 114 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 539 1017 1423 1512
Size: 287 252 478 406 89
Fragments arranged by size:
478 406 287 252 89
EcoO109I rG'GnC_Cy
Cuts at: 0 1027 1512
Size: 1027 485
EcoRI G'AATT_C
Cuts at: 0 467 1512
Size: 467 1045
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 631 1512
Size: 631 881
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 712 744 1512
Size: 181 32 499 32 768
Fragments arranged by size:
768 499 181 32 32
HincII GTy'rAC
Cuts at: 0 921 1512
Size: 921 591
HindIII A'AGCT_T
Cuts at: 0 253 1512
Size: 253 1259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 300 367 914 1512
Size: 98 202 67 547 598
Fragments arranged by size:
598 547 202 98 67
MscI TGG'CCA
Cuts at: 0 755 1387 1512
Size: 755 632 125
Fragments arranged by size:
755 632 125
MslI CAynn'nnrTG
Cuts at: 0 836 1021 1421 1512
Size: 836 185 400 91
Fragments arranged by size:
836 400 185 91
NheI G'CTAG_C
Cuts at: 0 360 1512
Size: 360 1152
NspI r_CATG'y
Cuts at: 0 232 353 996 1338 1512
Size: 232 121 643 342 174
Fragments arranged by size:
643 342 232 174 121
PciI A'CATG_T
Cuts at: 0 349 992 1334 1512
Size: 349 643 342 178
Fragments arranged by size:
643 349 342 178
PflMI CCAn_nnn'nTGG
Cuts at: 0 478 1383 1512
Size: 478 905 129
Fragments arranged by size:
905 478 129
PshAI GACnn'nnGTC
Cuts at: 0 1440 1512
Size: 1440 72
PspOMI G'GGCC_C
Cuts at: 0 1026 1512
Size: 1026 486
SpeI A'CTAG_T
Cuts at: 0 141 1512
Size: 141 1371
SphI G_CATG'C
Cuts at: 0 232 1512
Size: 232 1280
SspI AAT'ATT
Cuts at: 0 135 1512
Size: 135 1377
StyI C'CwwG_G
Cuts at: 0 15 1402 1512
Size: 15 1387 110
Fragments arranged by size:
1387 110 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 300 1411 1512
Size: 300 1111 101
Fragments arranged by size:
1111 300 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 786 818 1512
Size: 786 32 694
Fragments arranged by size:
786 694 32
XmnI GAAnn'nnTTC
Cuts at: 0 614 1512
Size: 614 898
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApaI ApaLI ApoI
AseI AvrII BamHI BanI BanII BbsI BcgI BclI
BglII Bme1580I BmtI BpmI Bpu10I BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BstAPI BstXI BstYI DraI DraIII
EaeI EarI Eco57MI EcoO109I EcoRI FalI Hin4I HincII
HindIII MmeI MscI MslI NheI NspI PciI PflMI
PshAI PspOMI SpeI SphI SspI StyI TaqII TstI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AscI AsiSI AvaI BaeI BbeI BbvCI
BciVI BfrBI BglI BlpI BmgBI BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI
EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PpuMI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SrfI StuI SwaI TatI Tth111I XbaI XcmI
XhoI XmaI ZraI