(Linear) (Six-base) MAPSORT of: KIEE5636.seq  Check: 4127  from: 1  to: 1446



KIEE5636.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:04  ..

Acc65I G'GTAC_C

Cuts at:      0     448    1446 
   Size:        448     998
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85    1446 
   Size:         85    1361
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     116    1446 
   Size:        116    1330
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1353    1385    1446 
   Size:       1353      32      61

  Fragments arranged by size: 

                1353      61      32
 
ApoI r'AATT_y

Cuts at:      0     253     898    1012    1446 
   Size:        253     645     114     434

  Fragments arranged by size: 

                 645     434     253     114
 
AvaI C'yCGr_G

Cuts at:      0      10      28     733    1446 
   Size:         10      18     705     713

  Fragments arranged by size: 

                 713     705      18      10
 
BanI G'GyrC_C

Cuts at:      0     448    1446 
   Size:        448     998
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     694    1031    1446 
   Size:        694     337     415

  Fragments arranged by size: 

                 694     415     337
 
BsaAI yAC'GTr

Cuts at:      0     565    1446 
   Size:        565     881
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1353    1383    1392    1422    1446 
   Size:       1353      30       9      30      24

  Fragments arranged by size: 

                1353      30      30      24       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     157     232     376     379    1053    1413    1446 
   Size:        157      75     144       3     674     360      33

  Fragments arranged by size: 

                 674     360     157     144      75      33       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     660    1446 
   Size:        660     786
 
BsmI GAATG_Cn'

Cuts at:      0     841    1446 
   Size:        841     605
 
BsrDI GCAATG_nn'

Cuts at:      0     810    1446 
   Size:        810     636
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     414    1446 
   Size:        414    1032
 
Bsu36I CC'TnA_GG

Cuts at:      0     796    1446 
   Size:        796     650
 
BtgI C'CryG_G

Cuts at:      0     476    1446 
   Size:        476     970
 
BtsI GCAGTG_nn'

Cuts at:      0     643     898     972    1446 
   Size:        643     255      74     474

  Fragments arranged by size: 

                 643     474     255      74
 
EarI CTCTTCn'nnn_

Cuts at:      0     134     173     272     353    1446 
   Size:        134      39      99      81    1093

  Fragments arranged by size: 

                1093     134      99      81      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     694    1031    1446 
   Size:         85     609     337     415

  Fragments arranged by size: 

                 609     415     337      85
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      67    1446 
   Size:         67    1379
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1379    1446 
   Size:       1379      67
 
EcoRI G'AATT_C

Cuts at:      0     253     898    1012    1446 
   Size:        253     645     114     434

  Fragments arranged by size: 

                 645     434     253     114
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     865    1446 
   Size:        865     581
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     405     437    1446 
   Size:        405      32    1009

  Fragments arranged by size: 

                1009     405      32
 
HindIII A'AGCT_T

Cuts at:      0     401    1446 
   Size:        401    1045
 
KpnI G_GTAC'C

Cuts at:      0     452    1446 
   Size:        452     994
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     419     806    1446 
   Size:        419     387     640

  Fragments arranged by size: 

                 640     419     387
 
MspA1I CmG'CkG

Cuts at:      0     776     888    1446 
   Size:        776     112     558

  Fragments arranged by size: 

                 776     558     112
 
NspI r_CATG'y

Cuts at:      0     615    1446 
   Size:        615     831
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1353    1385    1446 
   Size:       1353      32      61

  Fragments arranged by size: 

                1353      61      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1379    1446 
   Size:       1379      67
 
PstI C_TGCA'G

Cuts at:      0     893    1446 
   Size:        893     553
 
PvuII CAG'CTG

Cuts at:      0     776     888    1446 
   Size:        776     112     558

  Fragments arranged by size: 

                 776     558     112
 
SfcI C'TryA_G

Cuts at:      0     522     889    1119    1446 
   Size:        522     367     230     327

  Fragments arranged by size: 

                 522     367     327     230
 
TatI w'GTAC_w

Cuts at:      0      50     677    1446 
   Size:         50     627     769

  Fragments arranged by size: 

                 769     627      50
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI     AloI     ApoI     AvaI     BanI     BpmI
    BsaAI    BsaXI    BseRI     BsgI     BsmI    BsrDI   BstAPI   Bsu36I
     BtgI     BtsI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
    Hin4I  HindIII     KpnI     MmeI   MspA1I     NspI     PpiI    PpuMI
     PstI    PvuII     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI