Restriction map for
pFN21AE5633
(Linear) (Six-base) MAPSORT of: KIEE5633.seq Check: 2578 from: 1 to: 1476
KIEE5633.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 172 1365 1476
Size: 172 1193 111
Fragments arranged by size:
1193 172 111
AclI AA'CG_TT
Cuts at: 0 571 1476
Size: 571 905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 571 1476
Size: 334 237 905
Fragments arranged by size:
905 334 237
AfeI AGC'GCT
Cuts at: 0 1444 1476
Size: 1444 32
AleI CACnn'nnGTG
Cuts at: 0 958 1476
Size: 958 518
AlwNI CAG_nnn'CTG
Cuts at: 0 301 1476
Size: 301 1175
ApoI r'AATT_y
Cuts at: 0 885 1476
Size: 885 591
AvaI C'yCGr_G
Cuts at: 0 944 1476
Size: 944 532
BanI G'GyrC_C
Cuts at: 0 139 160 508 724 829 1476
Size: 139 21 348 216 105 647
Fragments arranged by size:
647 348 216 139 105 21
BanII G_rGCy'C
Cuts at: 0 1277 1476
Size: 1277 199
BbsI GAAGACnn'nnnn_
Cuts at: 0 1098 1476
Size: 1098 378
BciVI GTATCCnnnnn_n'
Cuts at: 0 1405 1476
Size: 1405 71
BfrBI ATG'CAT
Cuts at: 0 1237 1476
Size: 1237 239
BglI GCCn_nnn'nGGC
Cuts at: 0 79 158 1476
Size: 79 79 1318
Fragments arranged by size:
1318 79 79
BglII A'GATC_T
Cuts at: 0 1071 1476
Size: 1071 405
Bme1580I G_kGCm'C
Cuts at: 0 750 832 965 1476
Size: 750 82 133 511
Fragments arranged by size:
750 511 133 82
BmgBI CAC'GTC
Cuts at: 0 987 1476
Size: 987 489
BmrI ACTGGGnnnn_n'
Cuts at: 0 226 339 1236 1476
Size: 226 113 897 240
Fragments arranged by size:
897 240 226 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 494 622 1138 1231 1476
Size: 303 191 128 516 93 245
Fragments arranged by size:
516 303 245 191 128 93
Bpu10I CC'TnA_GC
Cuts at: 0 1004 1446 1476
Size: 1004 442 30
Fragments arranged by size:
1004 442 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1316 1476
Size: 1316 160
BsaAI yAC'GTr
Cuts at: 0 120 315 1476
Size: 120 195 1161
Fragments arranged by size:
1161 195 120
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1159 1162 1429 1476
Size: 1159 3 267 47
Fragments arranged by size:
1159 267 47 3
BseYI C'CCAG_C
Cuts at: 0 86 90 251 263 898 1290 1476
Size: 86 4 161 12 635 392 186
Fragments arranged by size:
635 392 186 161 86 12 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1476
Size: 1066 410
BsiEI CG_ry'CG
Cuts at: 0 484 1103 1476
Size: 484 619 373
Fragments arranged by size:
619 484 373
BsiHKAI G_wGCw'C
Cuts at: 0 404 710 1476
Size: 404 306 766
Fragments arranged by size:
766 404 306
Bsp1286I G_dGCh'C
Cuts at: 0 404 710 750 832 965 1277 1476
Size: 404 306 40 82 133 312 199
Fragments arranged by size:
404 312 306 199 133 82 40
BspHI T'CATG_A
Cuts at: 0 647 1476
Size: 647 829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4 172 777 1365 1476
Size: 4 168 605 588 111
Fragments arranged by size:
605 588 168 111 4
BsrDI GCAATG_nn'
Cuts at: 0 464 1476
Size: 464 1012
BsrFI r'CCGG_y
Cuts at: 0 811 1476
Size: 811 665
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1380 1476
Size: 1380 96
BstEII G'GTnAC_C
Cuts at: 0 789 1476
Size: 789 687
BstXI CCAn_nnnn'nTGG
Cuts at: 0 594 1476
Size: 594 882
BstYI r'GATC_y
Cuts at: 0 52 929 1071 1476
Size: 52 877 142 405
Fragments arranged by size:
877 405 142 52
Bsu36I CC'TnA_GG
Cuts at: 0 736 1267 1476
Size: 736 531 209
Fragments arranged by size:
736 531 209
BtgI C'CryG_G
Cuts at: 0 107 669 695 1164 1388 1460 1476
Size: 107 562 26 469 224 72 16
Fragments arranged by size:
562 469 224 107 72 26 16
BtsI GCAGTG_nn'
Cuts at: 0 1160 1476
Size: 1160 316
DraIII CAC_nnn'GTG
Cuts at: 0 146 1476
Size: 146 1330
DrdI GACnn_nn'nnGTC
Cuts at: 0 361 1476
Size: 361 1115
EaeI y'GGCC_r
Cuts at: 0 104 481 777 1028 1476
Size: 104 377 296 251 448
Fragments arranged by size:
448 377 296 251 104
EagI C'GGCC_G
Cuts at: 0 481 1476
Size: 481 995
EarI CTCTTCn'nnn_
Cuts at: 0 1060 1476
Size: 1060 416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 334 494 571 622 1138 1231 1476
Size: 303 31 160 77 51 516 93 245
Fragments arranged by size:
516 303 245 160 93 77 51 31
EcoO109I rG'GnC_Cy
Cuts at: 0 150 1064 1476
Size: 150 914 412
Fragments arranged by size:
914 412 150
FspI TGC'GCA
Cuts at: 0 655 1373 1432 1476
Size: 655 718 59 44
Fragments arranged by size:
718 655 59 44
HaeII r_GCGC'y
Cuts at: 0 1446 1476
Size: 1446 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 212 244 1476
Size: 212 32 1232
Fragments arranged by size:
1232 212 32
HincII GTy'rAC
Cuts at: 0 171 396 1476
Size: 171 225 1080
Fragments arranged by size:
1080 225 171
MscI TGG'CCA
Cuts at: 0 779 1476
Size: 779 697
MslI CAynn'nnrTG
Cuts at: 0 646 649 958 1242 1476
Size: 646 3 309 284 234
Fragments arranged by size:
646 309 284 234 3
MspA1I CmG'CkG
Cuts at: 0 21 90 671 783 1050 1284 1476
Size: 21 69 581 112 267 234 192
Fragments arranged by size:
581 267 234 192 112 69 21
NcoI C'CATG_G
Cuts at: 0 695 1476
Size: 695 781
NsiI A_TGCA'T
Cuts at: 0 1239 1476
Size: 1239 237
NspI r_CATG'y
Cuts at: 0 1180 1409 1476
Size: 1180 229 67
Fragments arranged by size:
1180 229 67
PmlI CAC'GTG
Cuts at: 0 315 1476
Size: 315 1161
PpuMI rG'GwC_Cy
Cuts at: 0 1064 1476
Size: 1064 412
PsiI TTA'TAA
Cuts at: 0 1202 1476
Size: 1202 274
PstI C_TGCA'G
Cuts at: 0 788 1476
Size: 788 688
PvuII CAG'CTG
Cuts at: 0 90 783 1284 1476
Size: 90 693 501 192
Fragments arranged by size:
693 501 192 90
RsrII CG'GwC_CG
Cuts at: 0 1385 1476
Size: 1385 91
SacII CC_GC'GG
Cuts at: 0 672 1476
Size: 672 804
SapI GCTCTTCn'nnn_
Cuts at: 0 1060 1476
Size: 1060 416
ScaI AGT'ACT
Cuts at: 0 370 1476
Size: 370 1106
SfcI C'TryA_G
Cuts at: 0 784 1476
Size: 784 692
SmaI CCC'GGG
Cuts at: 0 946 1476
Size: 946 530
SmlI C'TyrA_G
Cuts at: 0 1331 1476
Size: 1331 145
StuI AGG'CCT
Cuts at: 0 224 1451 1476
Size: 224 1227 25
Fragments arranged by size:
1227 224 25
StyI C'CwwG_G
Cuts at: 0 74 135 512 695 1305 1476
Size: 74 61 377 183 610 171
Fragments arranged by size:
610 377 183 171 74 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1029 1476
Size: 1029 447
TatI w'GTAC_w
Cuts at: 0 368 1476
Size: 368 1108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 86 118 304 336 1341 1373 1476
Size: 86 32 186 32 1005 32 103
Fragments arranged by size:
1005 186 103 86 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 841 1476
Size: 841 635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 984 1023 1476
Size: 984 39 453
Fragments arranged by size:
984 453 39
XmaI C'CCGG_G
Cuts at: 0 944 1476
Size: 944 532
XmnI GAAnn'nnTTC
Cuts at: 0 302 1476
Size: 302 1174
Enzymes that do cut:
AarI AclI AcuI AfeI AleI AlwNI ApoI AvaI
BanI BanII BbsI BciVI BfrBI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EagI EarI Eco57MI EcoO109I FspI HaeII
Hin4I HincII MscI MslI MspA1I NcoI NsiI NspI
PmlI PpuMI PsiI PstI PvuII RsrII SacII SapI
ScaI SfcI SmaI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AflII AflIII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BclI BlpI BmtI BplI
BsaI BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BsmBI
BspEI BsrBI BsrGI BssHII BssSI BstBI BstZ17I BtgZI
ClaI CspCI DraI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PpiI
PshAI PspOMI PspXI PsrI PvuI SacI SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XhoI ZraI