Restriction map for
pFN21AE5632
(Linear) (Six-base) MAPSORT of: KIEE5632.seq Check: 5644 from: 1 to: 1470
KIEE5632.orf
With 182 enzymes: SgfI *
December 5, 2014 13:04 ..
AccI GT'mk_AC
Cuts at: 0 1465 1470
Size: 1465 5
Acc65I G'GTAC_C
Cuts at: 0 1104 1470
Size: 1104 366
AlwNI CAG_nnn'CTG
Cuts at: 0 544 1237 1261 1470
Size: 544 693 24 209
Fragments arranged by size:
693 544 209 24
ApoI r'AATT_y
Cuts at: 0 215 564 882 1470
Size: 215 349 318 588
Fragments arranged by size:
588 349 318 215
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 971 1004 1470
Size: 971 33 466
Fragments arranged by size:
971 466 33
BanI G'GyrC_C
Cuts at: 0 963 1104 1470
Size: 963 141 366
Fragments arranged by size:
963 366 141
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 937 1091 1126 1470
Size: 20 917 154 35 344
Fragments arranged by size:
917 344 154 35 20
BglII A'GATC_T
Cuts at: 0 829 1470
Size: 829 641
BmrI ACTGGGnnnn_n'
Cuts at: 0 1455 1470
Size: 1455 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1376 1470
Size: 1376 94
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 1109 1160 1470
Size: 47 1062 51 310
Fragments arranged by size:
1062 310 51 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 1234 1258 1470
Size: 1234 24 212
Fragments arranged by size:
1234 212 24
BsaAI yAC'GTr
Cuts at: 0 643 1470
Size: 643 827
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 45 75 1470
Size: 45 30 1395
Fragments arranged by size:
1395 45 30
BseYI C'CCAG_C
Cuts at: 0 1195 1335 1470
Size: 1195 140 135
Fragments arranged by size:
1195 140 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 1470
Size: 761 709
BsiHKAI G_wGCw'C
Cuts at: 0 674 1470
Size: 674 796
BsmI GAATG_Cn'
Cuts at: 0 665 1470
Size: 665 805
Bsp1286I G_dGCh'C
Cuts at: 0 674 1470
Size: 674 796
BsrDI GCAATG_nn'
Cuts at: 0 135 1470
Size: 135 1335
BssSI C'ACGA_G
Cuts at: 0 1205 1470
Size: 1205 265
BstXI CCAn_nnnn'nTGG
Cuts at: 0 731 1470
Size: 731 739
BstYI r'GATC_y
Cuts at: 0 829 1470
Size: 829 641
BtsI GCAGTG_nn'
Cuts at: 0 122 639 1319 1470
Size: 122 517 680 151
Fragments arranged by size:
680 517 151 122
DraI TTT'AAA
Cuts at: 0 353 1470
Size: 353 1117
DraIII CAC_nnn'GTG
Cuts at: 0 1207 1470
Size: 1207 263
EarI CTCTTCn'nnn_
Cuts at: 0 867 907 1301 1470
Size: 867 40 394 169
Fragments arranged by size:
867 394 169 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 427 1470
Size: 427 1043
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 1109 1160 1470
Size: 47 1062 51 310
Fragments arranged by size:
1062 310 51 47
EcoRV GAT'ATC
Cuts at: 0 282 1470
Size: 282 1188
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 644 1241 1470
Size: 644 597 229
Fragments arranged by size:
644 597 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 237 269 1176 1208 1470
Size: 30 207 32 907 32 262
Fragments arranged by size:
907 262 207 32 32 30
HincII GTy'rAC
Cuts at: 0 1466 1470
Size: 1466 4
HindIII A'AGCT_T
Cuts at: 0 346 1415 1470
Size: 346 1069 55
Fragments arranged by size:
1069 346 55
KpnI G_GTAC'C
Cuts at: 0 1108 1470
Size: 1108 362
MfeI C'AATT_G
Cuts at: 0 807 1470
Size: 807 663
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 1470
Size: 636 834
MspA1I CmG'CkG
Cuts at: 0 67 90 1101 1470
Size: 67 23 1011 369
Fragments arranged by size:
1011 369 67 23
NspI r_CATG'y
Cuts at: 0 1159 1470
Size: 1159 311
PasI CC'CwG_GG
Cuts at: 0 1270 1470
Size: 1270 200
PflMI CCAn_nnn'nTGG
Cuts at: 0 1027 1436 1470
Size: 1027 409 34
Fragments arranged by size:
1027 409 34
PfoI T'CCnGG_A
Cuts at: 0 145 1470
Size: 145 1325
PstI C_TGCA'G
Cuts at: 0 36 1257 1470
Size: 36 1221 213
Fragments arranged by size:
1221 213 36
PvuII CAG'CTG
Cuts at: 0 90 1101 1470
Size: 90 1011 369
Fragments arranged by size:
1011 369 90
SalI G'TCGA_C
Cuts at: 0 1464 1470
Size: 1464 6
SfcI C'TryA_G
Cuts at: 0 32 983 1253 1470
Size: 32 951 270 217
Fragments arranged by size:
951 270 217 32
SnaBI TAC'GTA
Cuts at: 0 643 1470
Size: 643 827
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 45 77 1470
Size: 45 32 1393
Fragments arranged by size:
1393 45 32
XmnI GAAnn'nnTTC
Cuts at: 0 1416 1470
Size: 1416 54
Enzymes that do cut:
AccI Acc65I AlwNI ApoI BaeI BanI BbsI BglII
BmrI BplI BpmI BsaI BsaAI BsaXI BseYI BsgI
BsiHKAI BsmI Bsp1286I BsrDI BssSI BstXI BstYI BtsI
DraI DraIII EarI EciI Eco57MI EcoRV FalI Hin4I
HincII HindIII KpnI MfeI MmeI MspA1I NspI PasI
PflMI PfoI PstI PvuII SalI SfcI SnaBI TstI
XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI
Bpu10I BpuEI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DrdI EaeI EagI EcoICRI EcoNI EcoO109I EcoRI
FseI FspI FspAI HaeII HpaI KasI MluI MscI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmaI ZraI